scholarly journals Hyaluronic Acid and Wound Healing

2015 ◽  
Vol 18 (1) ◽  
pp. 53 ◽  
Author(s):  
Manuela G Neuman ◽  
Radu M Nanau ◽  
Loida Oruña-Sanchez ◽  
Gabriel Coto
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Wound Repair ◽  
Original Work ◽  
Human Keratinocytes ◽  
Review Of The Literature ◽  
Skin Repair ◽  
Normal Human Keratinocytes ◽  
Normal Human

Background. We developed an experimental model of ethanol-induced dermatotoxicity and hepatocytoxicity using normal human keratinocytes and normal human hepatocytes that preserve inducible cytochrome p450 activities. The original work was described in several articles. The objective of this study was to determine whether hyaluronic acid attenuates skin necrosis, and to further clarify its uses in wound repair in humans, animal models and in vitro studies. Methods. We performed a systematic review of the literature using the terms “hyaluronic acid” and “wound healing”. PubMed was searched for studies published during the period 2010-2014. Results. Hyaluronic acid is used in tissue regeneration alone or in combination with herbal or Western medicine. Scaffolds made up of hyaluronic acid were used to embed basic fibroblast growth factor. Conclusion. Hyaluronic acid extracts are safe and efficacious products to be used in skin repair. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

Electrospinning of collagen nanofibers: Effects on the behavior of normal human keratinocytes and early-stage wound healing

Biomaterials ◽  
2006 ◽  
Vol 27 (8) ◽  
pp. 1452-1461 ◽  
Author(s):  
Kyong Su Rho ◽  
Lim Jeong ◽  
Gene Lee ◽  
Byoung-Moo Seo ◽  
Yoon Jeong Park ◽  
...  
Keyword(s):  
Wound Healing ◽  
Early Stage ◽  
Human Keratinocytes ◽  
Normal Human Keratinocytes ◽  
Normal Human

scholarly journals Transforming growth factor-beta 1 modulates beta 1 and beta 5 integrin receptors and induces the de novo expression of the alpha v beta 6 heterodimer in normal human keratinocytes: implications for wound healing.

1995 ◽  
Vol 129 (3) ◽  
pp. 853-865 ◽  
Author(s):  
G Zambruno ◽  
P C Marchisio ◽  
A Marconi ◽  
C Vaschieri ◽  
A Melchiori ◽  
...  
Keyword(s):  
Wound Healing ◽  
De Novo ◽  
Human Keratinocytes ◽  
General Mechanism ◽  
Integrin Expression ◽  
Tgf Beta ◽  
Fibronectin Receptor ◽  
Receptor Alpha ◽  
Normal Human Keratinocytes ◽  
Normal Human

The molecular mechanism underlying the promotion of wound healing by TGF-beta 1 is incompletely understood. We report that TGF-beta 1 regulates the regenerative/migratory phenotype of normal human keratinocytes by modulating their integrin receptor repertoire. In growing keratinocyte colonies but not in fully stratified cultured epidermis, TGF-beta 1: (a) strongly upregulates the expression of the fibronectin receptor alpha 5 beta 1, the vitronectin receptor alpha v beta 5, and the collagen receptor alpha 2 beta 1 by differentially modulating the synthesis of their alpha and beta subunits; (b) downregulates the multifunctional alpha 3 beta 1 heterodimer; (c) induces the de novo expression and surface exposure of the alpha v beta 6 fibronectin receptor; (d) stimulates keratinocyte migration toward fibronectin and vitronectin; (e) induces a marked perturbation of the general mechanism of polarized domain sorting of both beta 1 and beta 4 dimers; and (f) causes a pericellular redistribution of alpha v beta 5. These data suggest that alpha 5 beta 1, alpha v beta 6, and alpha v beta 5, not routinely used by keratinocytes resting on an intact basement membrane, act as "emergency" receptors, and uncover at least one of the molecular mechanisms responsible for the peculiar integrin expression in healing human wounds. Indeed, TGF-beta 1 reproduces the integrin expression pattern of keratinocytes located at the injury site, particularly of cells in the migrating epithelial tongue at the leading edge of the wound. Since these keratinocytes are inhibited in their proliferative capacity, these data might account for the apparent paradox of a TGF-beta 1-dependent stimulation of epidermal wound healing associated with a growth inhibitory effect on epithelial cells.

scholarly journals Expression of C4.4A in an In Vitro Human Tissue-Engineered Skin Model

2017 ◽  
Vol 2017 ◽  
pp. 1-9
Author(s):  
Benedikte Jacobsen ◽  
Danielle Larouche ◽  
Olivier Rochette-Drouin ◽  
Michael Ploug ◽  
Lucie Germain
Keyword(s):  
Self Assembly ◽  
Human Keratinocytes ◽  
Skin Substitutes ◽  
Pathological Conditions ◽  
Monolayer Cultures ◽  
Normal Human Keratinocytes ◽  
Stratum Spinosum ◽  
Normal Human ◽  
Suprabasal Keratinocytes

A multi-LU-domain-containing protein denoted C4.4A exhibits a tightly regulated membrane-associated expression in the suprabasal layers of stratified squamous epithelia such as skin and the esophagus, and the expression of C4.4A is dysregulated in various pathological conditions. However, the biological function of C4.4A remains unknown. To enable further studies, we evaluated the expression of C4.4A in monolayer cultures of normal human keratinocytes and in tissue-engineered skin substitutes (TESs) produced by the self-assembly approach, which allow the formation of a fully differentiated epidermis tissue. Results showed that, in monolayer, C4.4A was highly expressed in the centre of keratinocyte colonies at cell-cell contacts areas, while some cells located at the periphery presented little C4.4A expression. In TES, emergence of C4.4A expression coincided with the formation of the stratum spinosum. After the creation of a wound within the TES, C4.4A expression was observed in the suprabasal keratinocytes of the migrating epithelium, with the exception of the foremost leading keratinocytes, which were negative for C4.4A. Our results are consistent with previous data in mouse embryogenesis and wound healing. Based on these findings, we conclude that this human TES model provides an excellent surrogate for studies of C4.4A and Haldisin expressions in human stratified epithelia.

scholarly journals Ascorbic Acid, Ultraviolet C Rays, and Glucose but not Hyperthermia Are Elicitors of Humanβ-Defensin 1 mRNA in Normal Keratinocytes

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Luis Antonio Cruz Díaz ◽  
María Guadalupe Flores Miramontes ◽  
Paulina Chávez Hurtado ◽  
Kirk Allen ◽  
Marisela Gonzalez Ávila ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ascorbic Acid ◽  
Human Keratinocytes ◽  
Independent Manner ◽  
Innate Defense ◽  
Normal Keratinocytes ◽  
Normal Human Keratinocytes ◽  
Ultraviolet C ◽  
Normal Human

Hosts’ innate defense systems are upregulated by antimicrobial peptide elicitors (APEs). Our aim was to investigate the effects of hyperthermia, ultraviolet A rays (UVA), and ultraviolet C rays (UVC) as well as glucose and ascorbic acid (AA) on the regulation of humanβ-defensin 1 (DEFB1), cathelicidin (CAMP), and interferon-γ(IFNG) genes in normal human keratinocytes (NHK). The indirectin vitroantimicrobial activity againstStaphylococcus aureusandListeria monocytogenesof these potential APEs was tested. We found that AA is a more potent APE forDEFB1than glucose in NHK. Glucose but not AA is an APE forCAMP. Mild hypo- (35°C) and hyperthermia (39°C) are not APEs in NHK. AA-dependentDEFB1upregulation below 20 mM predictsin vitroantimicrobial activity as well as glucose- and AA-dependentCAMPandIFNGupregulation. UVC upregulatesCAMPandDEFB1genes but UVA only upregulates theDEFB1gene. UVC is a previously unrecognized APE in human cells. Our results suggest that glucose upregulatesCAMPin an IFN-γ-independent manner. AA is an elicitor of innate immunity that will challenge the current concept of late activation of adaptive immunity of this vitamin. These results could be useful in designing new potential drugs and devices to combat skin infections.

scholarly journals A Novel Three-Polysaccharide Blend In Situ Gelling Powder for Wound Healing Applications

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1680
Author(s):  
Chiara Amante ◽  
Tiziana Esposito ◽  
Pasquale Del Del Gaudio ◽  
Veronica Di Di Sarno ◽  
Amalia Porta ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Healing Process ◽  
Antimicrobial Properties ◽  
Human Keratinocytes ◽  
Wound Healing Process ◽  
Host Matrix ◽  
In Situ Gelling

In this paper, alginate/pectin and alginate/pectin/chitosan blend particles, in the form of an in situ forming hydrogel, intended for wound repair applications, have been successfully developed. Particles have been used to encapsulate doxycycline in order to control the delivery of the drug, enhance its antimicrobial properties, and the ability to inhibit host matrix metalloproteinases. The presence of chitosan in the particles strongly influenced their size, morphology, and fluid uptake properties, as well as drug encapsulation efficiency and release, due to both chemical interactions between the polymers in the blend and interactions with the drug demonstrated by FTIR studies. In vitro antimicrobial studies highlighted an increase in antibacterial activity related to the chitosan amount in the powders. Moreover, in situ gelling powders are able to induce a higher release of IL-8 from the human keratinocytes that could stimulate the wound healing process in difficult-healing. Interestingly, doxycycline-loaded particles are able to increase drug activity against MMPs, with good activity against MMP-9 even at 0.5 μg/mL over 72 h. Such results suggest that such powders rich in chitosan could be a promising dressing for exudating wounds.

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