scholarly journals Luminescence-Based Acute Toxicity Bioassay for Heavy Metals Using Insoluble-Fibroin-Film-Immobilized Vibrio fischeri

2012 ◽  
pp. 261
2014 ◽  
Vol 6 (18) ◽  
pp. 7367-7373 ◽  
Author(s):  
Andressa Adame ◽  
Fábio R. P. Rocha

Fast determination of acute toxicity using the V. fischeri bacteria, including in-line sample dilutions and conditioning.


2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Francisco Martín ◽  
Marlon Escoto ◽  
Juan Fernández ◽  
Emilia Fernández ◽  
Elena Arco ◽  
...  

The potential toxicity in riverbed sediments was assessed with a bioassay using the bioluminescent bacteriaVibrio fischeri. The selected area was characterized by the presence of ultramafic rocks (peridotites), and the sediments had high values in Ni, Cr, and Co. For the toxicity bioassay withVibrio fischeri, water-soluble forms were used. The results indicated that most of the samples had a very low degree of toxicity, with 10% of reduction in luminescence in relation to the control; meanwhile 25% of the samples had a moderate degree of toxicity with a reduction in luminescence between 13 and 21% in relation to the control. The toxicity index correlated significantly with the concentrations of Ni and Cr in the water extracts. This toxicity bioassay was proved to be a sensitive and useful tool to detect potential toxicity in solutions, even with anomalous concentrations in heavy metals of natural origin.


2014 ◽  
Vol 6 (1) ◽  
pp. 117-120 ◽  
Author(s):  
S.S. Gandhewar ◽  
S.B. Zade ◽  
S.R. Sitre

Acute toxicity bioassay of three different heavy metals copper (Cu), lead (Pb) and cadmium (Cd) for the freshwater catfish, Clarias batrachus were conducted for evaluation of the toxicity. The 96h LC50 values recorded for Cu, Pb and Cd were 15 ppm, 29 ppm and 35 ppm respectively. The results of this study indicate that the order of toxicity of these heavy metals is Cu>Pb>Cd in short duration acute toxicity experiments. The toxicity was found to be dependent on dose and duration of experiment for all these heavy metals. Cu was found to be much more toxic than Pb and Cd to C. batrachus.


2015 ◽  
Vol 212 ◽  
pp. 352-359 ◽  
Author(s):  
Ouahid Ben Ghanem ◽  
Nicolas Papaiconomou ◽  
M.I. Abdul Mutalib ◽  
Sylvie Viboud ◽  
Mohanad El-Harbawi ◽  
...  

2016 ◽  
Vol 81 (6) ◽  
pp. 697-705 ◽  
Author(s):  
Xuepeng Yang ◽  
Yan Ji ◽  
Fangfang Wang ◽  
Jia Xu ◽  
Xiangzhen Liu ◽  
...  

Vibrio fischeri bioluminescence inhibition has been widely used to test acute toxicities of metals and organics contaminants. However, the differences of metals and organics acute toxicities to V. fischeri have not been compared. Here, four heavy metals (Zn2+, Cu2+, Cd2+, Cr6+) and five organics (phenol, benzoic acid, p-hydroxy benzoic acid, nitro-benzene and benzene) acute toxicities to V. fischeri were investigated. Heavy metals toxicities to V. fischeri were increased along with the reaction time, while the organics toxicities kept the same level in different reaction times. In order to explain the difference, the relative cell death rate of V. fischeri was detected. In metals toxicities tests, the bioluminescence inhibition rate of V. fischeri was found to be significantly higher than the relative cell death rate (P<0.05), while for the organics toxicities tests, the cell death rate was similar to the bioluminescence inhibition rate. These results indicated that organics acute toxicities to V. fischeri could reflect the death of cell, but metals acute toxicities to V. fischeri may not lead to the death of cell, just represent the bioluminescence inhibition.


2011 ◽  
Vol 88 (2) ◽  
pp. 290-295 ◽  
Author(s):  
E. Jurado ◽  
M. Fernández-Serrano ◽  
J. Núñez Olea ◽  
M. Lechuga ◽  
J. L. Jiménez ◽  
...  

Chemosphere ◽  
2019 ◽  
Vol 221 ◽  
pp. 45-54 ◽  
Author(s):  
Patrick Morkus ◽  
Mehdi Zolfaghari ◽  
Damien Parrello ◽  
Matthew Csordas ◽  
Mikayil Malikov ◽  
...  

2018 ◽  
Vol 7 (3) ◽  
pp. 82
Author(s):  
Lina Maria Caceres Diaz ◽  
Claudia Campos ◽  
Gideon Oron

This study examines the prospects for using Hydra viridissima toxicity test 96-h LC50, served as a model invertebrate, Lactuca sativa L. was applied for seeds toxicity test (120-h IC50) and a model plant for an acute toxicity assessment of heavy metals content in water. The heavy metals used to assess the acute toxicity of the water utilized for agricultural irrigation in arid regions includes cadmium (CdCl2.2H2O), chromium (K2Cr2O7), zinc (ZnSO4. 7H2O), and boron (H3BO3). A grading of the substances was conducted, and it was found that the toxicity levels for H. viridissima and L. sativa were, with the least harmful first: B < Cr < Zn < Cd and Zn < B < Cr < Cd, respectively. Results indicate that H. viridissima was a more sensitive indicator of toxicity for all of the evaluated substances. However, L. sativa could also be used successfully to rank toxicants in order of their potential hazards.


Sign in / Sign up

Export Citation Format

Share Document