Complicated bilateral fungal emphysematous pyelonephritis in a 55-year-old diabetic female: A case report and review of literature

Background and Purpose: Emphysematous pyelonephritis (EPN) is a rare and serious disease causing acute renal failure. Diabetes is a major risk factor for this infection.Case report: Herein, we present the case of a 55-year-old female patient with diabetes and EPN caused by Candida albicans. The infection was complicated with endophthalmitis and endocarditis. The results of antifungal susceptibility analysis showed that C. albicans was resistant to fluconazole and susceptible to amphotericin-B and itraconazole. Infection could be controlled by amphotericin-B followed by itraconazole therapy, and the patient was discharged in good condition while receiving antifungal therapy. Conclusion: Complicated pyelonephritis with unusual microorganisms should be considered in patients with diabetes and urinary symptoms. Keywords: Candida albicans, Emphysematous pyelonephritis

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Flow Cytometry Antifungal Susceptibility Testing of Pathogenic Yeasts other than Candida albicans and Comparison with the NCCLS Broth Microdilution Test

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.44.10.2752-2758.2000 ◽

2000 ◽

Vol 44 (10) ◽

pp. 2752-2758 ◽

Cited By ~ 44

Author(s):

Rama Ramani ◽

Vishnu Chaturvedi

Keyword(s):

Flow Cytometry ◽

Candida Albicans ◽

Amphotericin B ◽

Susceptibility Testing ◽

Antifungal Susceptibility ◽

Antifungal Susceptibility Testing ◽

Broth Microdilution ◽

Nitrogen Base ◽

Microdilution Method ◽

Broth Microdilution Method

ABSTRACT Candida species other than Candida albicansfrequently cause nosocomial infections in immunocompromised patients. Some of these pathogens have either variable susceptibility patterns or intrinsic resistance against common azoles. The availability of a rapid and reproducible susceptibility-testing method is likely to help in the selection of an appropriate regimen for therapy. A flow cytometry (FC) method was used in the present study for susceptibility testing ofCandida glabrata, Candida guilliermondii,Candida krusei, Candida lusitaniae,Candida parapsilosis, Candida tropicalis, andCryptococcus neoformans based on accumulation of the DNA binding dye propidium iodide (PI). The results were compared with MIC results obtained for amphotericin B and fluconazole using the NCCLS broth microdilution method (M27-A). For FC, the yeast inoculum was prepared spectrophotometrically, the drugs were diluted in either RPMI 1640 or yeast nitrogen base containing 1% dextrose, and yeast samples and drug dilutions were incubated with amphotericin B and fluconazole, respectively, for 4 to 6 h. Sodium deoxycholate and PI were added at the end of incubation, and fluorescence was measured with a FACScan flow cytometer (Becton Dickinson). The lowest drug concentration that showed a 50% increase in mean channel fluorescence compared to that of the growth control was designated the MIC. All tests were repeated once. The MICs obtained by FC for all yeast isolates except C. lusitaniae were in very good agreement (within 1 dilution) of the results of the NCCLS broth microdilution method. Paired ttest values were not statistically significant (P = 0.377 for amphotericin B; P = 0.383 for fluconazole). Exceptionally, C. lusitaniae isolates showed higher MICs (2 dilutions or more) than in the corresponding NCCLS broth microdilution method for amphotericin B. Overall, FC antifungal susceptibility testing provided rapid, reproducible results that were statistically comparable to those obtained with the NCCLS method.

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Candida albicans isolated from post-operative penetrating keratoplasty patient: Case report

Asian Journal of Medical Sciences ◽

10.3126/ajms.v5i3.8669 ◽

2014 ◽

Vol 5 (3) ◽

pp. 116-119

Author(s):

Abhishek Chandra ◽

Munesh Kumar Gupta ◽

Ragini Tilak

Keyword(s):

Candida Albicans ◽

Case Report ◽

Amphotericin B ◽

Penetrating Keratoplasty ◽

Antifungal Susceptibility ◽

Medical Science ◽

Eye Drops ◽

Gram Staining ◽

Chlamydospore Formation ◽

Culture Characteristics

We report a case report of Candida albicans suture infiltrate on 3rd post-op day in a 53 year female operated for penetrating keratoplasty. Candida albicans was identified by KOH mount, Gram Staining, germ tube, growth at 450C, chlamydospore formation and light green color on CHROMagar with sugar assimilation and culture characteristics. Despite being susceptible to Fluconazole by broth microdilution, patient did not respond to 0.3% fluconazole eye drops. On antifungal susceptibility testing by CLSI44A, it was susceptible to only Amphotericin B (100units). Patient was then started on 0.15% fortified amphotericin B eye drops resulting in complete resolution of infiltrates. Asian Journal of Medical Science, Volume-5(3) 2014: 116-119 http://dx.doi.org/10.3126/ajms.v5i3.8669

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Rhabdomyolysis associated acute renal failure – Report of two fatal cases and a brief review of literature

Journal of Forensic and Legal Medicine ◽

10.1016/j.jflm.2020.101941 ◽

2020 ◽

Vol 71 ◽

pp. 101941

Author(s):

Siddhartha Das ◽

Srinivas Bheemanathi Hanuman ◽

Jhansi Lakshmi Mylapalli

Keyword(s):

Renal Failure ◽

Acute Renal Failure ◽

Review Of Literature

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Dose-related effects of metformin on acid–base balance and renal function in patients with diabetes who develop acute renal failure: a cross-sectional study

Acta Diabetologica ◽

10.1007/s00592-016-0836-2 ◽

2016 ◽

Vol 53 (4) ◽

pp. 551-558 ◽

Cited By ~ 10

Author(s):

David Cucchiari ◽

Manuel Alfredo Podestà ◽

Elisa Merizzoli ◽

Albania Calvetta ◽

Emanuela Morenghi ◽

...

Keyword(s):

Renal Failure ◽

Renal Function ◽

Acute Renal Failure ◽

Cross Sectional Study ◽

Sectional Study ◽

Acid Base Balance ◽

Acid Base ◽

Cross Sectional ◽

Patients With Diabetes ◽

Base Balance

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Candida albicans isolated from denture-related stomatitis in elderly patients: Antifungal susceptibility and production of virulence attributes

Experimental Results ◽

10.1017/exp.2020.49 ◽

2020 ◽

Vol 1 ◽

Author(s):

Lourimar Viana Nascimento Franco de Sousa ◽

Carlos Davi de Oliveira Maia ◽

Isadora Sousa Carvalho ◽

Juliano Meireles Prata ◽

Larissa Carla Rodrigues Arcanjo ◽

...

Keyword(s):

Candida Albicans ◽

Elderly Patients ◽

Amphotericin B ◽

Antifungal Susceptibility ◽

Burning Sensation ◽

Aspartic Protease ◽

Elderly Individuals ◽

Polystyrene Surface ◽

Low Prevalence ◽

Effective Drugs

AbstractDenture-related stomatitis caused by Candida spp. affects elderly individuals using partial/total prosthesis, provoking several discomforts including burning sensation and altered taste. Herein, we have studied 52 denture-wearing individuals (>60 years-old), attended at the dentistry clinic of UNIVALE, aiming to isolate Candida spp. directly from the stomatitis lesions and to evaluate their potential to produce virulence attributes. A low prevalence of denture-related stomatitis was reported in these patients (4/52; 7.7%). Candida albicans was isolated in the 4 selected patients, with the ability to form biofilm over a polystyrene surface and to produce aspartic protease, esterase and hemolysin. However, neither phospholipase nor caseinase activities were detected. Planktonic-growing yeasts were susceptible to amphotericin B and caspofungin, while the susceptibility to azoles (fluconazol, itraconazole and voriconazole) varied depending on either the isolate or antifungal. Relevantly, biofilm-forming C. albicans cells exhibited resistance to all studied antifungals. So, new effective drugs against resistant C. albicans isolates causing denture-related stomatitis are urgently required.

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Emphysematous Pyelonephritis with Acute Renal Failure

Urology ◽

10.1016/j.urology.2008.02.076 ◽

2008 ◽

Vol 72 (3) ◽

pp. 521-522 ◽

Cited By ~ 3

Author(s):

Shiu-Dong Chung ◽

Chun-Hou Liao ◽

Hsu-Dong Sun ◽

Wen-Ching Wen

Keyword(s):

Renal Failure ◽

Acute Renal Failure ◽

Emphysematous Pyelonephritis

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Antifungal susceptibility of Candida albicans isolated from oral mucosa of patients with cancer

Revista de Odontologia da Universidade de São Paulo ◽

10.1590/s0103-06631999000300003 ◽

1999 ◽

Vol 13 (3) ◽

pp. 219-223 ◽

Cited By ~ 1

Author(s):

Lino João da COSTA ◽

Esther Goldenberg BIRMAN ◽

Sidney Hartz ALVES ◽

Arlete Emily CURY

Keyword(s):

Candida Albicans ◽

Cancer Patients ◽

Amphotericin B ◽

Oral Mucosa ◽

Antifungal Susceptibility ◽

New Drugs ◽

Local Conditions ◽

Opportunistic Fungi ◽

Sabouraud Agar

The increasing number of oral infections due to opportunistic fungi in immunocompromised patients, needs a new evaluation of the drugs in use. The susceptibility in vitro of Candida albicans strains from the oral mucosa of cancer patients to amphotericin B, ketoconazole, miconazole, fluconazole and itraconazole were evaluated. A dilution technique in YNB agar or subculture on Sabouraud agar was utilised for MIC or MFC determinations. With this methodology, the best fungicidal drug for C.albicans collected from the oral mucosa of 40 cancer patients, divided in two groups: one treated by radiotherapy and the other not, the best results were obtained with amphotericin B, presenting low values of MIC compared to azoles and MFC values. However it is important to take into consideration the utilisation in vivo of this polyenic antibiotic and the possible toxic levels necessary to achieve good results. The coexistence of other fungi and the local conditions must also be pondered with these patients, who are mostly undergoing radiotherapy. In order to achieve better results without undesirable consequences, higher levels of MIC are expected with the daily clinical use of new drugs.

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