scholarly journals Candida albicans isolated from post-operative penetrating keratoplasty patient: Case report

2014 ◽  
Vol 5 (3) ◽  
pp. 116-119
Author(s):  
Abhishek Chandra ◽  
Munesh Kumar Gupta ◽  
Ragini Tilak
Keyword(s):  
Candida Albicans ◽  
Case Report ◽  
Amphotericin B ◽  
Penetrating Keratoplasty ◽  
Antifungal Susceptibility ◽  
Medical Science ◽  
Eye Drops ◽  
Gram Staining ◽  
Chlamydospore Formation ◽  
Culture Characteristics

We report a case report of Candida albicans suture infiltrate on 3rd post-op day in a 53 year female operated for penetrating keratoplasty. Candida albicans was identified by KOH mount, Gram Staining, germ tube, growth at 450C, chlamydospore formation and light green color on CHROMagar with sugar assimilation and culture characteristics. Despite being susceptible to Fluconazole by broth microdilution, patient did not respond to 0.3% fluconazole eye drops. On antifungal susceptibility testing by CLSI44A, it was susceptible to only Amphotericin B (100units). Patient was then started on 0.15% fortified amphotericin B eye drops resulting in complete resolution of infiltrates. Asian Journal of Medical Science, Volume-5(3) 2014: 116-119 http://dx.doi.org/10.3126/ajms.v5i3.8669 

scholarly journals Flow Cytometry Antifungal Susceptibility Testing of Pathogenic Yeasts other than Candida albicans and Comparison with the NCCLS Broth Microdilution Test

2000 ◽  
Vol 44 (10) ◽  
pp. 2752-2758 ◽  
Author(s):  
Rama Ramani ◽  
Vishnu Chaturvedi
Keyword(s):  
Flow Cytometry ◽  
Candida Albicans ◽  
Amphotericin B ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Broth Microdilution ◽  
Nitrogen Base ◽  
Microdilution Method ◽  
Broth Microdilution Method

ABSTRACT Candida species other than Candida albicansfrequently cause nosocomial infections in immunocompromised patients. Some of these pathogens have either variable susceptibility patterns or intrinsic resistance against common azoles. The availability of a rapid and reproducible susceptibility-testing method is likely to help in the selection of an appropriate regimen for therapy. A flow cytometry (FC) method was used in the present study for susceptibility testing ofCandida glabrata, Candida guilliermondii,Candida krusei, Candida lusitaniae,Candida parapsilosis, Candida tropicalis, andCryptococcus neoformans based on accumulation of the DNA binding dye propidium iodide (PI). The results were compared with MIC results obtained for amphotericin B and fluconazole using the NCCLS broth microdilution method (M27-A). For FC, the yeast inoculum was prepared spectrophotometrically, the drugs were diluted in either RPMI 1640 or yeast nitrogen base containing 1% dextrose, and yeast samples and drug dilutions were incubated with amphotericin B and fluconazole, respectively, for 4 to 6 h. Sodium deoxycholate and PI were added at the end of incubation, and fluorescence was measured with a FACScan flow cytometer (Becton Dickinson). The lowest drug concentration that showed a 50% increase in mean channel fluorescence compared to that of the growth control was designated the MIC. All tests were repeated once. The MICs obtained by FC for all yeast isolates except C. lusitaniae were in very good agreement (within 1 dilution) of the results of the NCCLS broth microdilution method. Paired ttest values were not statistically significant (P = 0.377 for amphotericin B; P = 0.383 for fluconazole). Exceptionally, C. lusitaniae isolates showed higher MICs (2 dilutions or more) than in the corresponding NCCLS broth microdilution method for amphotericin B. Overall, FC antifungal susceptibility testing provided rapid, reproducible results that were statistically comparable to those obtained with the NCCLS method.

scholarly journals Candida albicans isolated from denture-related stomatitis in elderly patients: Antifungal susceptibility and production of virulence attributes

2020 ◽  
Vol 1 ◽  
Author(s):  
Lourimar Viana Nascimento Franco de Sousa ◽  
Carlos Davi de Oliveira Maia ◽  
Isadora Sousa Carvalho ◽  
Juliano Meireles Prata ◽  
Larissa Carla Rodrigues Arcanjo ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Elderly Patients ◽  
Amphotericin B ◽  
Antifungal Susceptibility ◽  
Burning Sensation ◽  
Aspartic Protease ◽  
Elderly Individuals ◽  
Polystyrene Surface ◽  
Low Prevalence ◽  
Effective Drugs

AbstractDenture-related stomatitis caused by Candida spp. affects elderly individuals using partial/total prosthesis, provoking several discomforts including burning sensation and altered taste. Herein, we have studied 52 denture-wearing individuals (>60 years-old), attended at the dentistry clinic of UNIVALE, aiming to isolate Candida spp. directly from the stomatitis lesions and to evaluate their potential to produce virulence attributes. A low prevalence of denture-related stomatitis was reported in these patients (4/52; 7.7%). Candida albicans was isolated in the 4 selected patients, with the ability to form biofilm over a polystyrene surface and to produce aspartic protease, esterase and hemolysin. However, neither phospholipase nor caseinase activities were detected. Planktonic-growing yeasts were susceptible to amphotericin B and caspofungin, while the susceptibility to azoles (fluconazol, itraconazole and voriconazole) varied depending on either the isolate or antifungal. Relevantly, biofilm-forming C. albicans cells exhibited resistance to all studied antifungals. So, new effective drugs against resistant C. albicans isolates causing denture-related stomatitis are urgently required.

Antibacterial Efficacy of the Compound Preparation of Traditional Chinese Drug on System Fungi Reside in Faecal Specimen of Pigeon

2012 ◽  
Vol 518-523 ◽  
pp. 5514-5517 ◽  
Author(s):  
Lian Lan Ma ◽  
Zhi Chun Liu ◽  
Xiao Yuan Wang ◽  
Xiao Yun Wu
Keyword(s):  
Candida Albicans ◽  
Cryptococcus Neoformans ◽  
Drug Concentration ◽  
Chinese Drug ◽  
Faecal Specimen ◽  
Sterile Saline ◽  
Gram Staining ◽  
Chlamydospore Formation ◽  
Positive Rate ◽  
Acid Test

To investigate the efficacy of the compound preparation of traditional Chinese drug which is named KZY-2) on system fungi reside in faecal specimen of pigeon, the 66 faecal specimen of pigeon were collected from columbary of local park and residents.Each specimen which weigh 2.0g was mixed with 10ml sterile saline and the supernatant which volume was 1ml was incubated with the same volume drug which concentration were 200mɡ/ml,100mɡ/ml and 50mɡ/ml respectively at 37°C.After 24h,48h and 72h, the isolated positive rates of Cryptococcus neoformans and Candida albicans were calculated. Candida albicans was identified by gram staining, budding development, chlamydospore formation, sugar fermented test,sugar assimilated test and Cryptococcus neoformans was identified by gram staining,sugar fermented test,sugar assimilated test,urease tests,caffeic acid test, Hiss capsule staining.Results showed that the isolated positive rates of Cryptococcus neoformans and Candida albicans were 27.3%（18/66） and 16.7%（11/66） without drug action, KZY-2 could reduce the isolated positive rates of Cryptococcus neoformans and Candida albicans(P<0.05 and P<0.05).The drug concentration and action time could significantly affect the isolated positive rate which was negative correlation between them, higher drug concentration and longer incubation time,more lower the positive rate, especially the isolated positive rates of Cryptococcus neoformans are reduced 77.7%（6.1/27.3）which incubated with 200mɡ/ml drug after 72h compared with the control group.These results indicate The KZY-2 have good germicidal efficacy on Cryptococcus neoformans and Candida albicans isolated from faecal specimen of pigeon.

scholarly journals Antifungal susceptibility of Candida albicans isolated from oral mucosa of patients with cancer

1999 ◽  
Vol 13 (3) ◽  
pp. 219-223 ◽  
Author(s):  
Lino João da COSTA ◽  
Esther Goldenberg BIRMAN ◽  
Sidney Hartz ALVES ◽  
Arlete Emily CURY
Keyword(s):  
Candida Albicans ◽  
Cancer Patients ◽  
Amphotericin B ◽  
Oral Mucosa ◽  
Antifungal Susceptibility ◽  
New Drugs ◽  
Local Conditions ◽  
Opportunistic Fungi ◽  
Sabouraud Agar

The increasing number of oral infections due to opportunistic fungi in immunocompromised patients, needs a new evaluation of the drugs in use. The susceptibility in vitro of Candida albicans strains from the oral mucosa of cancer patients to amphotericin B, ketoconazole, miconazole, fluconazole and itraconazole were evaluated. A dilution technique in YNB agar or subculture on Sabouraud agar was utilised for MIC or MFC determinations. With this methodology, the best fungicidal drug for C.albicans collected from the oral mucosa of 40 cancer patients, divided in two groups: one treated by radiotherapy and the other not, the best results were obtained with amphotericin B, presenting low values of MIC compared to azoles and MFC values. However it is important to take into consideration the utilisation in vivo of this polyenic antibiotic and the possible toxic levels necessary to achieve good results. The coexistence of other fungi and the local conditions must also be pondered with these patients, who are mostly undergoing radiotherapy. In order to achieve better results without undesirable consequences, higher levels of MIC are expected with the daily clinical use of new drugs.

scholarly journals 618. Fluconazole-Resistant Candida albicans Vaginitis with Cross-Resistance to Azoles: A Case Report

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S287-S288
Author(s):  
Damla Akdağ ◽  
Hüsnü Pullukçu ◽  
Tansu Yamazhan ◽  
Dilek Yesim Metin ◽  
Oğuz Reşat Sipahi ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Case Report ◽  
Clinical Response ◽  
Antifungal Susceptibility ◽  
Vaginal Swab ◽  
Vulvovaginal Candidiasis ◽  
Vaginal Candidiasis ◽  
Cross Resistance ◽  
High Dose

Abstract Background Local and systemic use of azole derivatives are common in the treatment of vulvovaginal candidiasis. However, there are cases unresponsive to these agents. Herein, we present treatment and follow-up of a patient with fluconazole–itraconazole and voriconazole-resistant recurrent vaginal candidiasis. Methods A 37-year-old woman with no comorbidity used topical and oral antifungal/antibacterial medications (including fluconazole and itraconazole) in the treatment of recurrent vulvovaginitis, was hospitalized due to continuous complaints. Intense, white-colored, odorless vaginal discharge was observed on physical examination. Urine and vaginal swab samples were taken for mycological and bacteriological culture. Metronidazole (500 mg 3x1 i.v.) and high dose fluconazole (600 mg/day i.v.) were initiated empirically for the possibility of dose-dependent resistant Candida infection, but there was no clinical response. Results Candida albicans was isolated in vaginal swab culture, but response to systemic fluconazole treatment for one week was inadequate. Antifungal susceptibility test was performed by microdilution method according to CLSI M27A3 guidelines and MIC values were reported respectively; fluconazole 4 µg/mL (SDD), itraconazole 1 µg/mL (R), posaconazole 0.06 µg/mL (WT), voriconazole 0.25 µg/mL (SDD), anidulafungin ≤ 0.015 µg/mL (S), amphotericin B 0.06 µg/mL (WT). For the resistance mechanism, point mutation in the ERG11 gene and MDR1 and MDR2 from efflux pumps were investigated and only the G464S mutation was detected in the ERG11 gene. Treatment was switched to IV anidulafungin (200 mg on day 1 followed by 100 mg/day). Clinical response was achieved in the patient whose complaints were reduced, and there was no Candida in the repeated vaginal swab culture taken on day 3 of treatment. The patient was discharged after 2 weeks of treatment. She had no recurrence after 2 years follow-up. Conclusion It should be kept in mind that resistant strains may be responsible for recurrent and unresponsive vulvovaginal candidiasis cases. Although there is no case report in which anidulafungin is used for treatment and it should be kept in mind that the anidulafungin is also in the treatment as it is summarized. Disclosures All authors: No reported disclosures.

scholarly journals Genetic diversity and antifungal susceptibility of Candida albicans isolates from Iranian HIV-infected patients with oral candidiasis

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Iradj Ashrafi Tamai ◽  
Babak Pakbin ◽  
Bahar Nayeri Fasaei
Keyword(s):  
Genetic Diversity ◽  
Candida Albicans ◽  
Amphotericin B ◽  
Random Amplified Polymorphic Dna ◽  
Oral Candidiasis ◽  
Antifungal Susceptibility ◽  
Diffusion Method ◽  
Glucose Agar ◽  
Sabouraud Glucose Agar ◽  
Inhibition Zones

Abstract Objective The objectives of this study were to investigate the antifungal susceptibility and genetic diversity of Candida albicans isolated from HIV+ patients with oropharyngeal candidiasis. A total of 50 C. albicans isolates were cultured on Sabouraud glucose agar containing chloramophenicol. The antifungal susceptibility of the isolates against fluconazole, clotrimazole, nystatin, amphotericin B, ketoconazole and flucytosine was assessed using disc diffusion method. The genetic diversity of C. albicans isolates was determined using random amplified polymorphic DNA marker. Results The inhibition zones ranged from 4 ± 1.8 to 40 ± 3.8 mm for fluconazole, 7 ± 1.0 to 37 ± 1.8 mm for ketoconazole, 14 ± 0.8 to24 ± 0.8 mm for amphotericin B, 25 ± 0.0 to 33 ± 0.0 mm for nystatin and 7 ± 4.2 to 40 ± 0.0 mm for clotrimazole. At 90% similarity, three distinct groups were observed. The smallest cluster composed of 3 isolates, whereas the largest one composed of 17 isolates. 32% (16/50), 28% (14/50) and 14% (7/50) were resistant to fluconazole, ketoconazole and clotrimazole, respectively.

scholarly journals Saprochaete clavata (Geotrichum clavatum) septicemia in a patient with multiple myeloma; An emerging case from Southeastern Turkey

2021 ◽  
Author(s):  
Handan Kangul ◽  
Nida Ozcan ◽  
Nurullah Uzuner ◽  
Mahmut Mete ◽  
Ufuk Mert Erginer
Keyword(s):  
Multiple Myeloma ◽  
Case Report ◽  
Amphotericin B ◽  
Fungal Infections ◽  
Antifungal Susceptibility ◽  
Autologous Bone Marrow Transplantation ◽  
Autologous Bone Marrow ◽  
Blue Staining ◽  
Current Case ◽  
Minimal Inhibitory Concentrations

Background and Purpose: Invasive fungal infections (IFI) are life-threatening and can be seen in immuno-compromised patients with malignancy, those who undergo chemotherapy, or transplant recipients. The Candida and Aspergillus species are the most common IFI agents; however, infections can also be caused by rare fungal species. This case report is about a bloodstream infection due to Saprochaete clavata (formerly known as Geotrichum clavatum) in a woman with multiple myeloma. Case report: A 59-years-old woman suffered from fever, widespread rashes, and diarrhea after an autologous bone marrow transplantation. Peripheral blood cultures were taken from the patient and sent to the microbiology laboratory. Cultures grew white to cream-colored cottony colonies. Moreover, septate and branched hyphae and arthroconidia were seen under a microscope by lactophenol blue staining. The fungi colonies were identified by Maldi Biotyper 3. 1. (manufactured by Bruker Daltonics, USA) as S. clavata (G. clavatum) with a reliable score. Antifungal susceptibility test was carried out by the concentration gradient strip Etest method. Minimal inhibitory concentrations of Amphotericin B, fluconazole, voriconazole, posaconazole, and anidulafungin were determined as 4, 3, 0.125, 0.125, and > 32 mg/dL, respectively. Despite amphotericin B treatment, the patient died three days after the identification of the fungi. Conclusion: The IFIs are serious conditions that have high mortality rates. In the current case report, we aimed to draw attention to S. clavata which is a rare fungal agent.

scholarly journals Complicated bilateral fungal emphysematous pyelonephritis in a 55-year-old diabetic female: A case report and review of literature

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Ensieh Lotfali ◽  
Sara Abolghasemi ◽  
Fatemeh Sadat Gatmirimotahhari ◽  
Mohammad Alizadeh ◽  
Zahra Arab-Mazar
Keyword(s):  
Renal Failure ◽  
Acute Renal Failure ◽  
Candida Albicans ◽  
Amphotericin B ◽  
Antifungal Susceptibility ◽  
Good Condition ◽  
Review Of Literature ◽  
Emphysematous Pyelonephritis ◽  
Patients With Diabetes ◽  
Serious Disease

Background and Purpose: Emphysematous pyelonephritis (EPN) is a rare and serious disease causing acute renal failure. Diabetes is a major risk factor for this infection.Case report: Herein, we present the case of a 55-year-old female patient with diabetes and EPN caused by Candida albicans. The infection was complicated with endophthalmitis and endocarditis. The results of antifungal susceptibility analysis showed that C. albicans was resistant to fluconazole and susceptible to amphotericin-B and itraconazole. Infection could be controlled by amphotericin-B followed by itraconazole therapy, and the patient was discharged in good condition while receiving antifungal therapy. Conclusion: Complicated pyelonephritis with unusual microorganisms should be considered in patients with diabetes and urinary symptoms. Keywords: Candida albicans, Emphysematous pyelonephritis

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