scholarly journals Histamine (H1) Receptors, Cyclooxygenase Pathway and Nitric Oxide Formation Involved in Rat Tracheal Smooth Muscle Relaxant Effect of Berberine

2019 ◽  
Author(s):  
Saeideh Saadat ◽  
Farzaneh Naghdi ◽  
Vahideh Ghorani ◽  
Hassan Rakhshandeh ◽  
Mohammad Hossein Boskabady
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Positive Control ◽  
Negative Control ◽  
Tracheal Smooth Muscle ◽  
Oxide Formation ◽  
Relaxant Effect ◽  
Nitric Oxide Formation ◽  
Significant Difference ◽  
Rat Tracheal Smooth Muscle

In this study we aimed to examine the relaxant effect of berberine, a compound extracted from a variety of herbs, on rat tracheal smooth muscle (TSM) and its possible mechanism(s). Cumulative concentrations of berberine (20, 65, 200 and 600 μg/mL) were added on pre-contracted TSM by methacholine or KCl in non-incubated or incubated tissues with atropine, chlorpheniramine, propranolol, diltiazem, glibenclamide, indomethacin, L-NG-nitro arginine methyl ester (L-NAME) and papaverine. The relaxant effects of theophylline (0.2, 0.4, 0.6 and 0.8 mM) as positive control and saline (1 mL) as negative control were also examined in non-incubated tissues. Berberine showed significant and concentration-dependent relaxant effects in non-incubated tissues contracted by KCl and methacholine (p<0.01 to p<0.001). There was no significant difference in the relaxant effects of berberine between non-incubated and incubated tissues with atropine, propranolol, diltiazem, glibenclamide, and papaverine. The relaxant effects of second concentrations of berberine in incubated tissues with L-NAME, its three lower concentration in incubated tissues with chlorpheniramine and its all concentrations in incubated tissues with indomethacin were significantly lower than non-incubated tissues (p<0.05 to p<0.001). The EC50 values of berberine in incubated tissues with chlorpheniramine was significantly higher than the non-incubated condition (p<0.05). Our findings reveal a relatively potent relaxant effect of berberine that is lower than the effect of theophylline. Proposed mechanisms for the relaxant effect of berberine are histamine (H1) receptor blockade, inhibition of cyclooxygenase pathways and/or nitric oxide formation.  

scholarly journals Relaxant Effect of Urginea maritima on Tracheal Smooth Muscle Mediated by the Effect on Beta-2 Adrenergic, Muscarinic Receptors and Calcium and Potassium Channels

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Hamideh Kazemi Rad ◽  
Arghavan Memarzia ◽  
Fatemeh Amin ◽  
Mohammad Hossein Boskabady
Keyword(s):  
Smooth Muscle ◽  
Fungal Infections ◽  
Positive Control ◽  
Negative Control ◽  
Tracheal Smooth Muscle ◽  
Organ Bath ◽  
Relaxant Effect ◽  
Urginea Maritima ◽  
Male Wistar Rats ◽  
Beta 2

Urginea maritima (U. maritima) showed anti-inflammatory, antioxidant, antibacterial, diuretic, vasodilatation, and wound-healing effects on fungal infections, cardiac disorders, digestive disorders, rheumatoid disease, and respiratory disorders such as bronchitis, bronchial nosocomial infections, and severe cough. To examine the bronchodilatory effect of U. maritima, the relaxant effect of its extract on rat tracheal smooth muscle (TSM) and its possible mechanism was examined in this study. Male Wistar rats’ TSM were divided into eight groups (n = 8 in each group). Four of these groups were TSM tissues, contracted with KCl (60 mM) incubated with atropine, glibenclamide, and indomethacin and nonincubated TSM, while the other four groups were TSM tissues contracted with methacholine (10 μM) for 5 min, incubated with propranolol, chlorpheniramine, and diltiazem and nonincubated TSM. Cumulative concentrations of U. maritima extract (12.5, 25, 50, 100, 20, and 400 μg/ml) were then added to organ bath every 5 min. Theophylline (0.2, 0.4, 0.6, and 0.8 mM) as positive control and saline (1 ml) as negative control were also examined in nonincubated tissues. A concentration-dependent relaxant effect of U. maritima on nonincubated TSM contracted with KCl (60 mM) or methacholine (10 μM) ( p < 0.01 and p < 0.001 ) was observed. The relaxant effects of U. maritima extract in the incubated tissues with glibenclamide, propranolol, diltiazem, atropine, and chlorpheniramine were significantly lower than those in the nonincubated tissues ( p < 0.05 to p < 0.001 ). EC50 values of U. maritima extract in the incubated TSM with glibenclamide, propranolol, diltiazem, and atropine were significantly higher than those in the nonincubated tissues ( p < 0.05 for diltiazem-incubated tissues and p < 0.001 for other cases). U. maritima extract displayed considerable relaxant effect on TSM comparable to the effect of theophylline. Beta-2 adrenoceptor stimulation and muscarinic receptor inhibition as well as potassium opening and calcium channels blocking effects are the possible mechanisms for the relaxant effects of the plant.

scholarly journals Relaxant effect of Curcuma longa on rat tracheal smooth muscle and its possible mechanisms

2017 ◽  
Vol 55 (1) ◽  
pp. 2248-2258 ◽  
Author(s):  
Bahman Emami ◽  
Farzaneh Shakeri ◽  
Vahideh Ghorani ◽  
Mohammad Hossein Boskabady
Keyword(s):  
Smooth Muscle ◽  
Curcuma Longa ◽  
Tracheal Smooth Muscle ◽  
Relaxant Effect ◽  
Rat Tracheal Smooth Muscle

scholarly journals Evaluation of cytotoxicity of two endodontic cements in a macrophage culture

2005 ◽  
Vol 13 (3) ◽  
pp. 237-242 ◽  
Author(s):  
Celso Emanoel de Souza Queiroz ◽  
Janir Alves Soares ◽  
Renato de Toledo Leonardo ◽  
Iracilda Zeponi Carlos ◽  
Welington Dinelli
Keyword(s):  
Nitric Oxide ◽  
Cell Culture ◽  
Tissue Culture ◽  
Epoxy Resin ◽  
Peritoneal Macrophages ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Significant Difference

Compared to gutta-percha, the endodontic cements are used in small quantity to seal root canals, but are indispensable to achieve hermetically sealed margins, where its biocompatibility depends on the sum of responses of each cell present in the periapical region. The object of this study was to evaluate the cytotoxicity of two endodontic cements, one based on epoxy resin (Sealer 26) and the other containing zinc oxide eugenol (Endofill) by using cultured peritoneal macrophages from Swiss mice to measure the induced production of nitric oxide. After solidification and pulverization, aliquots of 100mul of suspension containing 18mg/mL of the respective cements were added to 96-well tissue culture plates containing the tissue culture of macrophages at a concentration of 5.0X10(6) cells/ml. In the positive control group the cell culture was treated with 10mg/mL of lipopolyssaccharide from Escherichia coli 026:B6 and the cell culture alone represented the negative control. After 48 hours of incubation, at 37ºC, in 5% CO2, the cultures were placed in an ELISA automatic reader to evaluate the release of nitric oxide. The production of nitric oxide for cement Sealer 26 was between 36.1 and 313.0 mumols, with a mean of 143.82±111.03mumols, while for the Endofill these values were significantly less (p=0.01), varying from 50.8 to 125.7mumols, with a mean of 80.33±28.42 mumols. In the positive and negative control groups the mean release of nitric oxide was of 162.75mumols and 4.42mumols, respectively. There was no significant difference between the positive control group and cement Sealer 26 (p>0.05). Therefore, the cement Sealer 26 caused significantly greater toxicity to the macrophages, possibly due to the components from the epoxy resin and formaldehyde release during polymerization.

Mechanisms of relaxant activity of the nitric oxide-independent soluble guanylyl cyclase stimulator BAY 41-2272 in rat tracheal smooth muscle

2010 ◽  
Vol 645 (1-3) ◽  
pp. 158-164 ◽  
Author(s):  
Haroldo A. Toque ◽  
Fabíola Z.T. Mónica ◽  
Rafael P. Morganti ◽  
Gilberto De Nucci ◽  
Edson Antunes
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Guanylyl Cyclase ◽  
Soluble Guanylyl Cyclase ◽  
Tracheal Smooth Muscle ◽  
Rat Tracheal Smooth Muscle
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