scholarly journals Echinococcus granulosus sensu stricto in Livestock and Hu-man in Hamadan, Western Iran

2019 ◽  
Author(s):  
Mohammad MATINI ◽  
Mohammad FALLAH ◽  
Amir Hossein MAGHSOOD ◽  
Massoud SAIDIJAM ◽  
Majid FASIHI HARANDI
Keyword(s):  
Hydatid Cyst ◽  
Pcr Amplification ◽  
Sensu Stricto ◽  
Genetic Identification ◽  
Nucleotide Sequencing ◽  
Hydatid Cysts ◽  
High Genetic Diversity ◽  
Genetic Characteristics ◽  
Western Iran ◽  
Transmission Cycle

Background: Cystic echinococcosis, a major public health and economic concern, is a zoonotic helminth infection with worldwide distribution. This study was conducted to investigate the genetic characteristics of hydatid cysts isolated from human and livestock in Hamadan region, western Iran during 2016-2017. Methods: Ten human hydatid cysts and 40 animal hydatid cysts including 32 sheep, 5 cattle and 3 goats were genotyped by PCR amplification of two mitochondrial genes, cox1 and nad1. Genetic identification of the isolates was performed by using bioinformatics software and mtDNA nucleotide sequences of the parasite, available in GenBank database. Results: The PCR amplification was successfully carried out on 50 hydatid cyst isolates and then the nucleotide sequencing was conducted. The sequence analysis of the samples found that the isolates belonged to E. granulosus sensu stricto including G1 (42/50, 84%), G2 (4/50, 8%) and G3 (4/50, 8%) genotype. The G1 genotype was detected in human (8/10, 80%), sheep (26/32, 81%), cattle (5/5, 100%) and goat (3/3, 100%) hydatid cysts. The G2 and G3 genotypes were found only in sheep and human isolates. Alignment analysis of the cox1 and nad1 gene sequences revealed thirteen and ten sequence types, respectively. Conclusion: G1 was the prevailing genotype of E. granulosus in the area and dog-sheep transmission cycle should be considered when implementing hydatidosis control programs. In addition, high genetic diversity was detected among the hydatid cyst isolates.

scholarly journals Epidemiological, Morphometric, and Molecular Investigation of Cystic Echinococcosis in Camel and Cattle From Upper Egypt: Current Status and Zoonotic Implications

2021 ◽  
Vol 8 ◽  
Author(s):  
Ahmed Gareh ◽  
Amira A. Saleh ◽  
Samar M. Moustafa ◽  
Amin Tahoun ◽  
Roua S. Baty ◽  
...  
Keyword(s):  
Hydatid Cyst ◽  
Molecular Identification ◽  
Cystic Echinococcosis ◽  
Current Status ◽  
Economic Losses ◽  
Organ Distribution ◽  
Hydatid Cysts ◽  
Transmission Cycle ◽  
Parasitic Zoonoses ◽  
Female Cattle

Cystic echinococcosis has been considered one of the major parasitic zoonoses which is associated with severe economic losses. The present study was undertaken to investigate the occurrence, organ distribution, cyst fertility, and viability of cystic echinococcosis in slaughtered camels and cattle from various abattoirs in Assiut Governorate, Egypt. The work also involved morphological, morphometric, and molecular identification of the parasite. The occurrence of hydatid cysts was investigated in total number of 100 lungs of camels and 574 liver and lungs of cattle admitted to three slaughterhouses at Assiut Governorate, Egypt. Moreover, several individual variable factors, including organ involvement, age, sex, and hydatid cyst characteristics, were studied to identify their possible association with the occurrence of the disease. Genomic DNA was extracted from the hydatid cysts, followed by molecular identification of the parasite through amplification of ribosomal DNA internal transcribed spacer (ITS) regions. Hydatid cysts were found in 6 camels (6%) out of 100 inspected camels, while 5 hydatid cysts (0.87%) were detected in a total number of 574 cattle examined. The parasite was detected exclusively in lungs of camels, while lungs were the main organ infected by the parasite in cattle and one hydatid cyst was found in the liver (0.17%). In camel, 66.7, 16.65, and 16.65%of detected cysts were fertile, sterile, and calcified, respectively, while in cattle, these percentages were 60, 20, and 20%, respectively. None of the studied variable factors were significantly associated with the occurrence of the disease in camels, with the exception that all cysts were found in the lung. Conversely, we found a significant association (P < 0.05) between the age and sex of the slaughtered cattle and the occurrence of hydatid cysts. In this respect, the rate of infection was higher in female cattle and those cattle more than 5 years (P < 0.05). The morphological, morphometric, and molecular studies confirmed the presence of the parasite. Taken together, our results concluded that camels and cattle play a potential role in maintaining the transmission cycle of this zoonotic parasite.

scholarly journals Genetic characterization of hydatid cysts of different intermediate hosts

2020 ◽  
Vol 57 (3) ◽  
pp. 185-195
Author(s):  
W. M. Mousa ◽  
A. M. Abdel-Wahab ◽  
M. El-Gameel Sohila ◽  
O. A. Mahdy
Keyword(s):  
Pcr Amplification ◽  
Genetic Mutation ◽  
Sensu Stricto ◽  
Nucleotide Sequences ◽  
Hydatid Cysts ◽  
Intermediate Hosts ◽  
Economic Problems ◽  
Animal Isolates ◽  
Cattle And Sheep

SummaryCystic echinococcosis is an important cosmopolitan parasitic zoonosis that causes public health and economic problems in Egypt. The present study was undertaken to identify genotypes of hydatid cyst (HC) DNA isolated from different animal isolates and to identify the genotype of secondary hydatid cysts (HCs) developed in rabbits experimentally infected with camel HC for detection of any genetic mutation. In the present study, we extracted DNA from the germinal layers of 8 HCs collected from 3 camels, 1 cattle, 1 sheep and 3 donkeys in addition to 3 secondary HCs collected from rabbits experimentally infected with camel HC. PCR amplification of the ITS1 gene of all examined samples showed an amplified DNA band at 1115 bp. The partial nucleotide sequences of the ITS1 gene of all isolates were aligned and compared with the reference sequences of the genotypes G1–G8 in GenBank. The camel and rabbit samples were identified as Echinococcus canadensis genotype 6 (G6), while the cattle and sheep samples belonged to E. granulosus sensu stricto (G1). The donkey isolates belonged to E. equines (G4). Alignment of the ITS1 partial nucleotide sequences of the camel HCs and rabbit secondary HCs isolates with the G6 partial nucleotide sequence in GenBank was performed. Both camel HCs and rabbit secondary HCs isolates exhibited the same sequence identity matrix, which indicated the absence of mutation in the rabbit secondary HCs. It can be concluded that camel and rabbit samples were identified as E. canadensis (G6), the cattle and sheep samples belonged to E. granulosus sensu stricto (G1) and donkey isolates belonged to E. equines (G4). No mutation occurred during HCs transmission from camel to rabbit.

scholarly journals Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 125
Author(s):  
Piero Bonelli ◽  
Silvia Dei Giudici ◽  
Angela Peruzzu ◽  
Lorena Mura ◽  
Cinzia Santucciu ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Animal Species ◽  
Cost Effective ◽  
Sensu Stricto ◽  
Sybr Green ◽  
Hydatid Cysts ◽  
Genotype Identification ◽  
Host Selectivity ◽  
Nad5 Gene ◽  
Echinococcus Granulosus Sensu Lato

Echinococcus granulosus sensu lato (s.l.) is the causative agent of cystic echinococcosis in animals and humans. Different E. granulosuss.l. genotypes exhibit great diversity in their life cycle, host selectivity and pathogenicity. For this reason, the study of genetic variation within Echinococcus species is of importance for their epidemiological implication. We employed two SNP genotyping technologies to distinguish G1 and G3 E. granulosus sensu stricto (s.s.). genotypes. The genotypes of DNA samples (n = 28) extracted from hydatid cysts of different animal species were identified by amplification and sequencing of a fragment of the mitochondrial nad5 gene. Two SYBR green and three TaqMan real time PCR assays were developed for targeting of three nad5 informative positions (SNP758, 1123, and 1380) known to be able to discriminate G1 from G3. Genotyping by SYBR Green PCR based on cycle threshold (Ct) with melting temperature (Tm) analysis and performed on SNP1123 and SNP1380 failed to identify one DNA sample. TaqMan assays for SNP758, 1123 and 1380 effectively confirmed genotype identification obtained by Sanger sequencing. Our results demonstrated that the combination of the three Taqman assays developed in this study represents a valuable and cost effective tool alternative to DNA sequencing for E. granulosus s.s. genotyping.

scholarly journals Conservative Surgical Management for Pulmonary Hydatid Cyst: Analysis and Outcome of 148 Cases

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Mohammed Aldahmashi ◽  
Mohamed Alassal ◽  
Ibrahim Kasb ◽  
Hany Elrakhawy
Keyword(s):  
Hydatid Cyst ◽  
Bronchopleural Fistula ◽  
Complete Removal ◽  
Conservative Surgery ◽  
Hydatid Cysts ◽  
Rural Regions ◽  
Purse String ◽  
Lung Cysts ◽  
Surgical Methods ◽  
Pulmonary Hydatid Cyst

Background. Hydatid cyst (HC) disease is endemic in many developing countries, like Yemen, Egypt, and Saudi Arabia, especially in the rural regions. The disease has a variable clinical courses and even might be asymptomatic for many years.Objectives. In giant and large pulmonary hydatid cysts, pulmonary resection is the usual method of surgical treatment. In this study, we aimed to evaluate the lung conservative surgery in treatment of cases with giant and large hydatid lung cysts, as an effective method of management.Patients and Methods. Between January 2009 and August 2014, a total of 148 patients with pulmonary hydatid cysts were operated and their data was reviewed retrospectively and analyzed. Out of these cases, 52 (35.14%) cysts with more than 10 cm in diameter and 36 (24.32%) cysts with 5–9 cm were regarded as giant and large hydatid lung cysts, respectively. The small cysts less than 5 cm were presented in 8 (5.4%) cases only; other cases had ruptured cysts. Preservation of the lung tissues during surgery by cystotomy and Capitonnage was our conservative surgical methods of choice.Results. Eight patients developed bronchopleural fistula (BPF); of them, 4 BPFs have healed with chest tube and physiotherapy, but in the other 4 patients reoperation was done for the closure of persistent BPF. No mortality was observed in the present study.Conclusion. We conclude that conservative surgical procedure can achieve complete removal of the pulmonary hydatid cyst. Enucleation of the intact huge cysts is safe. Careful and secured closure of the bronchial communication should be done by purse string or figure-of-8 sutures, with or without Teflon pledgets. These simple procedures are safe, reliable, and successful.

The use of computerized tomography in the diagnosis of cerebral hydatid cysts

1979 ◽  
Vol 50 (3) ◽  
pp. 339-342 ◽  
Author(s):  
Tuncalp Özgen ◽  
Aykut Erbengi ◽  
Vural Bertan ◽  
Süleyman Saǧlam ◽  
Özdemir Gürçay ◽  
...  
Keyword(s):  
Hydatid Cyst ◽  
Computerized Tomography ◽  
Ct Scanning ◽  
Hydatid Cysts ◽  
Content Type ◽  
Cerebral Hydatid Cyst ◽  
Cyst Membrane ◽  
Fine Print

✓ Eleven cases of cerebral hydatid cyst, diagnosed by computerized tomography (CT), are presented. The importance of CT in minimizing the possibility of accidentally tapping or tearing the cyst membrane is stressed. Repeat CT scanning after removal of the cyst revealed atrophy in the affected hemisphere.

Azole resistance among clinical isolates of Aspergillus fumigatus in Lima-Peru

2019 ◽  
Vol 58 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Beatriz Bustamante ◽  
Luis Ricardo Illescas ◽  
Andrés Posadas ◽  
Pablo E Campos
Keyword(s):  
Aspergillus Fumigatus ◽  
Latin American ◽  
Pcr Amplification ◽  
Sensu Stricto ◽  
Clinical Isolates ◽  
Azole Resistance ◽  
Molecular Testing ◽  
Naive Patient ◽  
In Vitro Susceptibility

Abstract Azole resistance among Aspergillus fumigatus isolates, which is mainly related to mutations in the cyp51A gene, is a concern because it is rising, worldwide disseminated, and associated with treatment failure and death. Data on azole resistance of aspergillus from Latin American countries is very scarce and do not exist for Peru. Two hundred and seven Aspergillus clinical isolates collected prospectively underwent mycology and molecular testing for specie identification, and 143 isolates were confirmed as A. fumigatus sensu stricto (AFSS). All AFSS were tested for in vitro azole susceptibility, and resistant isolates underwent PCR amplification and sequencing of the whole cyp51A gene and its promoter. The in vitro susceptibility showed a minimal inhibitory concentration (MIC) range, MIC50 and MIC90 of 0.125 to >16, 0.25, and 0.5 μg/ml for itraconazole; 0.25 to 2, 0.5, and 0.5 μg/ml for voriconazole; and 0.003 to 1, 0.06, and 0.125 μg/ml for posaconazole. Three isolates (2%) showed resistance to itraconazole and exhibited different mutations of the cyp51A gene. One isolate harbored the mutation M220K, while a second one exhibited the G54 mutation plus a modification in the cyp51A gene promoter. The third isolate, from an azole naive patient, presented an integration of a 34-bp tandem repeat (TR34) in the promoter region of the gene and a substitution of leucine 98 by histidine (L98H). The three source patients had a diagnosis or suspicion of chronic pulmonary aspergillosis.

A cardiac hydatid cyst mimicking a pericardial tumour in a paediatric case

2018 ◽  
Vol 29 (2) ◽  
pp. 244-246 ◽  
Author(s):  
Taliha Oner ◽  
Oktay Korun ◽  
Ahmet Celebi
Keyword(s):  
Hydatid Cyst ◽  
Cardiac Involvement ◽  
The Body ◽  
Hydatid Cysts ◽  
Common Site ◽  
Right Ventricular Free Wall ◽  
Rare Presentation ◽  
Paediatric Case ◽  
Involvement Rate ◽  
The Right

AbstractCardiac hydatid cysts are a rare presentation of hydatid cyst disease in the body, with a reported cardiac involvement rate of <2%. The left ventricle is the most common site of cardiac involvement. Here, we report a patient with a hydatid cyst that ruptured into the pericardium after producing an aneurysm on the right ventricular free wall, appearing as fibrinated fluid and a solid mass lesion in the pericardium. Our aim in this case report was to emphasise that the possibility of a hydatid cyst should not be overlooked in the differential diagnosis of pericardial tumours.

scholarly journals Detection and genetic identification of pestiviruses in Brazilian lots of fetal bovine serum collected from 2006 to 2014

2018 ◽  
Vol 38 (3) ◽  
pp. 387-392 ◽  
Author(s):  
Francielle L. Monteiro ◽  
Juliana F. Cargnelutti ◽  
Patrícia Braunig ◽  
Aurea V. Folgueras-Flatschart ◽  
Nathália C. Santos ◽  
...  
Keyword(s):  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Genetic Identification ◽  
Nucleotide Sequencing ◽  
Rt Pcr ◽  
Diarrhea Virus ◽  
Fetal Bovine ◽  
Viral Diarrhea Virus ◽  
Pcr Targeting

ABSTRACT: The present study performed a genetic identification of pestiviruses contaminating batches of fetal bovine serum (FBS) produced in Brazil from 2006 to 2014. Seventy-three FBS lots were screened by a RT-PCR targeting the 5’untranslated region (UTR) of the pestivirus genome. Thirty-nine lots (53.4%) were positive for pestivirus RNA and one contained infectious virus. Nucleotide sequencing and phylogenetic analysis of the 5’UTR revealed 34 lots (46.6%) containing RNA of bovine viral diarrhea virus type 1 (BVDV-1), being 23 BVDV-1a (5’ UTR identity 90.8-98.7%), eight BVDV-1b (93.9-96.7%) and three BVDV-1d (96.2- 97.6%). Six lots (8.2%) contained BVDV-2 (90.3-100% UTR identity) being two BVDV-2a; three BVDV-2b and one undetermined. Four FBS batches (5.5%) were found contaminated with HoBi-like virus (98.3 to 100%). Five batches (6.8%) contained more than one pestivirus. The high frequency of contamination of FBS with pestivirus RNA reinforce the need for systematic and updated guidelines for monitoring this product to reduce the risk of contamination of biologicals and introduction of contaminating agents into free areas.

scholarly journals Genotype Characterization of Human Hydatid Cyst Isolates From Patients in Karaj, Iran, Using COX1 Gene Sequence

2019 ◽  
Vol 7 (1) ◽  
pp. 27-29
Author(s):  
Maryam Salimi ◽  
Abolfazl Miahipour ◽  
Mohammad Zibaei ◽  
Sasan Rezaie
Keyword(s):  
Hydatid Cyst ◽  
Gene Sequence ◽  
Cox1 Gene ◽  
Zoonotic Infection ◽  
Pcr Assay ◽  
Genetic Characteristics ◽  
Control And Prevention ◽  
Polymerase Chain ◽  
Clinical Problems

Background: Cystic echinococcosis is a main zoonotic infection. It can cause serious clinical problems for human health around the world. Genotypic specification of Echinococcus granulosus in human is important due to control and prevention programs. Objective: In this investigation, genetic characteristics of human isolates of E. granulosus in Karaj, Iran, were studied. Materials and Methods: In this review, 3 isolates of surgically removed hydatid cysts were obtained from patients in Shahid Madani hospital, Karaj, Iran in 2014. DNA was extracted from the protoscolex of the cyst, and polymerase chain reaction (PCR) assay was done on the COX1 gene. Results: DNA fragments were sequenced and the results were aligned and analyzed. Among the isolates, 3 (100%) were E. granulosus (G1) strain. Conclusion: The G1 genotype was the most superior strain from human isolates of hydatid cyst in Karaj.

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