scholarly journals CARACTERÍSTICAS CULTURAIS E PATOLÓGICAS DE Bipolaris maydis EM DIFERENTES MEIOS DE CULTURA

2017 ◽  
Vol 15 (3) ◽  
pp. 461
Author(s):  
JULIANA SALTIRES SANTOS ◽  
ANTONIO TEIXEIRA DO AMARAL JÚNIOR ◽  
MARCELO VIVAS ◽  
JANIELI MAGANHA SILVA VIVAS ◽  
RAILAN NASCIMENTO FERREIRA KUROSAWA ◽  
...  
Keyword(s):  
Zea Mays ◽  
Mycelial Growth ◽  
Culture Medium ◽  
Zea Mays L ◽  
Culture Media ◽  
Potato Dextrose Agar ◽  
Bipolaris Maydis ◽  
Polished Rice ◽  
Number Of Leaves ◽  
Corn Leaf Blight

RESUMO A mancha de Bipolaris, causada por Bipolaris maydis, é uma das principais doenças foliares de Zea mays,apresentando um considerável potencial de dano, notadamente, na cultura do milho-pipoca, embora trabalhos sobreeste patossistema ainda sejam escassos. Neste trabalho, avaliaram-se as influências de diferentes meios de culturasobre o crescimento micelial, esporulação e a infectividade de inóculo conidial de B. maydis em folhas da cultivarsuscetível Beija-flor, de milho-pipoca. Para tal, instalaram-se experimentos em laboratório e em casa de vegetação emblocos casualizados com quatro repetições. Em laboratório, avaliaram-se o crescimento micelial e a esporulação de B.maydis em diferentes meios de culturas (Batata Dextrose Ágar, Feijão Ágar, Água de Coco Ágar, Arroz Polido Ágar,Folha de Milho Ágar e Lactose Caseína Ágar). Em casa de vegetação, avaliaram-se o número de lesões por folha e aincidência de doença na planta suscetível inoculada com uma suspensão de 104 conídios/mL. O meio de Arroz PolidoÁgar foi o que mais promoveu o crescimento micelial do fungo, porém não favoreceu a esporulação. Para esporulação,destacaram-se os meios de cultura Batata Dextrose Ágar e Feijão Ágar. Maior número de folhas com sintoma dadoença e maior número de lesões por folha foram obtidos com o inóculo produzido no meio Feijão Ágar.Palavras-Chave: Zea mays L., Crescimento micelial, Mancha de Bipolaris, Esporulação, Milho-pipoca.CULTURAL AND PATHOLOGICAL FEATURES OF Bipolaris maydis IN DIFFERENT CULTURE MEDIAABSTRACT. The Southern corn leaf blight, caused by Bipolaris maydis, is a major foliar disease of corn, presentingconsiderable potential of damage, especially in Zea mays, although studies with this pathosystem are still scarce. In thispaper, the effect of different culture media was evaluated on mycelial sporulation and infectivity of conidial inoculum ofB. maydis on leaves of the susceptible cultivar Beija-Flor, of corn-popcorn. Experiments in laboratory and greenhousewere installed in a randomized blocks design with four replications. The mycelial growth and sporulation of B. maydison different culture media (Potato Dextrose Agar, Bean Agar, Coconut Water Agar, Rice Polished Agar, Corn LeafAgar Lactose Casein Agar) were evaluated in the laboratory. In the greenhouse it was evaluated the number of lesionsper leaf and the incidence of disease in susceptible plants inoculated with a suspension of 104 conidia/ml. The culturemedium Polished Rice Agar promoted the highest mycelial growth, but did not promote sporulation. Sporulation washigher on culture media Potato dextrose Agar and Agar Bean. A greater number of leaves with symptoms of the diseaseand a higher number of lesions per leaf were obtained with the inoculum produced in the culture medium Bean Agar.Keywords: Zea mays L, Mycelial growth, Southern corn leaf bligh, Sporulation, Popcorn.

CARACTERÍSTICAS CULTURAIS E PATOLÓGICAS DE Bipolaris maydis EM DIFERENTES MEIOS DE CULTURA

2016 ◽  
Vol 15 (3) ◽  
pp. 461
Author(s):  
JULIANA SALTIRES SANTOS ◽  
ANTONIO TEIXEIRA DO AMARAL JÚNIOR ◽  
MARCELO VIVAS ◽  
JANIELI MAGANHA SILVA VIVAS ◽  
RAILAN NASCIMENTO FERREIRA KUROSAWA ◽  
...  
Keyword(s):  
Zea Mays ◽  
Mycelial Growth ◽  
Culture Medium ◽  
Zea Mays L ◽  
Culture Media ◽  
Potato Dextrose Agar ◽  
Bipolaris Maydis ◽  
Polished Rice ◽  
Number Of Leaves ◽  
Corn Leaf Blight

RESUMO A mancha de Bipolaris, causada por Bipolaris maydis, é uma das principais doenças foliares de Zea mays, apresentando um considerável potencial de dano, notadamente, na cultura do milho-pipoca, embora trabalhos sobre este patossistema ainda sejam escassos. Neste trabalho, avaliaram-se as influências de diferentes meios de cultura sobre o crescimento micelial, esporulação e a infectividade de inóculo conidial de B. maydis em folhas da cultivar suscetível Beija-flor, de milho-pipoca. Para tal, instalaram-se experimentos em laboratório e em casa de vegetação em blocos casualizados com quatro repetições. Em laboratório, avaliaram-se o crescimento micelial e a esporulação de B. maydis em diferentes meios de culturas (Batata Dextrose Ágar, Feijão Ágar, Água de Coco Ágar, Arroz Polido Ágar, Folha de Milho Ágar e Lactose Caseína Ágar). Em casa de vegetação, avaliaram-se o número de lesões por folha e a incidência de doença na planta suscetível inoculada com uma suspensão de 104 conídios/mL. O meio de Arroz Polido Ágar foi o que mais promoveu o crescimento micelial do fungo, porém não favoreceu a esporulação. Para esporulação, destacaram-se os meios de cultura Batata Dextrose Ágar e Feijão Ágar. Maior número de folhas com sintoma da doença e maior número de lesões por folha foram obtidos com o inóculo produzido no meio Feijão Ágar. Palavras-Chave: Zea mays L., Crescimento micelial, Mancha de Bipolaris, Esporulação, Milho-pipoca.CULTURAL AND PATHOLOGICAL FEATURES OF Bipolaris maydis IN DIFFERENT CULTURE MEDIAABSTRACT. The Southern corn leaf blight, caused by Bipolaris maydis, is a major foliar disease of corn, presenting considerable potential of damage, especially in Zea mays, although studies with this pathosystem are still scarce. In this paper, the effect of different culture media was evaluated on mycelial sporulation and infectivity of conidial inoculum of B. maydis on leaves of the susceptible cultivar Beija-Flor, of corn-popcorn. Experiments in laboratory and greenhouse were installed in a randomized blocks design with four replications. The mycelial growth and sporulation of B. maydis on different culture media (Potato Dextrose Agar, Bean Agar, Coconut Water Agar, Rice Polished Agar, Corn Leaf Agar Lactose Casein Agar) were evaluated in the laboratory. In the greenhouse it was evaluated the number of lesions per leaf and the incidence of disease in susceptible plants inoculated with a suspension of 104 conidia/ml. The culture medium Polished Rice Agar promoted the highest mycelial growth, but did not promote sporulation. Sporulation was higher on culture media Potato dextrose Agar and Agar Bean. A greater number of leaves with symptoms of the disease and a higher number of lesions per leaf were obtained with the inoculum produced in the culture medium Bean Agar.Keywords: Zea mays L, Mycelial growth, Southern corn leaf bligh, Sporulation, Popcorn.

Growth and sporulation in vitro of Cercospora apii, Cercospora arachidicola, Cercospora kikuchii, and other species of Cercospora

1982 ◽  
Vol 60 (6) ◽  
pp. 862-868 ◽  
Author(s):  
N. E. El-Gholl ◽  
S. A. Alfieri Jr. ◽  
W. H. Ridings ◽  
C. L. Schoulties
Keyword(s):  
Mycelial Growth ◽  
Culture Medium ◽  
Culture Media ◽  
Single Point ◽  
Potato Dextrose Agar ◽  
Multiple Point ◽  
Cercospora Arachidicola ◽  
Cercospora Kikuchii ◽  
Conidial Production

Cercospora apii Fres., Cercospora arachidicola Hori, and Cercospora kikuchii (Matsumoto & Tomoyasu) Gardn. were single spored, and the resultant colonies were characterized. All isolates of C. arachidicola were morphologically identical, whereas those of C. apii and C. kikuchii were either smooth or had radial folds. Variability in sporulation was also evident. Multiple-point inoculation of culture media in plates with conidia helped to overcome variability in the amount of conidial production. Maximum sporulation was obtained at 4 days with C. apii and C. kikuchii, and from 7 to 10 days with C. arachidicola. Single-point inoculation of a V-8 culture medium in plates from peripheral dark mycelial growth in potato dextrose agar (PDA) culture resulted in increased sporulation by C. kikuchii. Sporulation of C. kikuchii was maintained throughout a 15-month study by selective subculturing; however, conidial production decreased over time. A total of 15 other species of Cercospora sporulated within a week following a V-8 inoculation of culture medium in plates with mycelial agar blocks.

scholarly journals Growth and sporulation of Metarhizium flavoviride var. Flavoviride on culture media and lighting regimes

2001 ◽  
Vol 58 (3) ◽  
pp. 613-616 ◽  
Author(s):  
Sideney Becker Onofre ◽  
Cindia Mara Miniuk ◽  
Neiva Monteiro de Barros ◽  
João Lúcio Azevedo
Keyword(s):  
Biological Control ◽  
Mycelial Growth ◽  
Culture Media ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Continuous Illumination ◽  
Agricultural Pests ◽  
Dark Cycle ◽  
Metarhizium Flavoviride ◽  
Conidia Production

Entomopathogenic fungi from the genus Metarhizium are largely used for the biological control of agricultural pests by conidia spreading on the field. Although conidia production is well studied in M. anisopliae, only few research studies were done in M. flavoviride. The present work was carried out alming to evaluate the Mycelial growth and sporulation of the entomopathogenic fungus Metarhizium flavoviride var. flavoviride growing at 27 ± 2°C on Potato-dextrose-agar (PDA), Czapek-agar (CZP) and a complete agar medium (CM) under three lighting regimes, (continuous illumination, light/dark cycle and an black light/dark cycle) were investigated. A completely randomized 3 × 3 (culture media × lighting regime) factorial design with four replicates was used. The best mycelial growth and sporulation occurred on the PDA and CM media under continuous illumination (P <= 0,05).

scholarly journals MODIFICAÇÕES NO MEIO DE CULTURA, FOTOPERÍODO E TEMPO DE CULTIVO AFETAM O ALONGAMENTO E ENRAIZAMENTO IN VITRO DE BANANEIRA CV. PACOVAN

Nativa ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 27
Author(s):  
Marcos Vinícius Marques Pinheiro ◽  
Ana Cristina Portugal Pinto De Carvalho ◽  
Fabrina Bolzan Martins
Keyword(s):  
Plant Height ◽  
Culture Medium ◽  
Culture Media ◽  
Dry Weight ◽  
Salt Mixture ◽  
Musa Spp ◽  
Fresh Biomass ◽  
Shoot Dry Weight ◽  
Number Of Leaves

No intuito de elevar as taxas de sobrevivência durante a etapa de aclimatização e posterior plantio a campo, avaliou-se o enraizamento in vitro de bananeira cv. Pacovan, em diferentes concentrações de sais MS e de sacarose. Utilizou-se DIC, esquema fatorial (6x2x3), com seis meios de cultura [sendo três concentrações de nutrientes do meio MS (100%; 50% de macronutrientes; e 50% dos sais macro e micronutrientes), e duas concentrações de sacarose (1,5/3,0%)], dois fotoperíodos (12/16 h) e três tempos de cultivo (21, 28 ou 35 dias) e seis repetições/tratamento. Analisaram-se: altura da planta, número de folhas/planta, massas frescas e secas das partes aérea e radicular. Para altura da planta, massa fresca da parte aérea e radicular, o meio MS 50% dos sais + sacarose (1,5%) com fotoperíodo de 16 h e tempo de cultivo de 35 dias foi satisfatório. Para massa seca da parte aérea foi MS 50% de sais + sacarose (3%), e para massa seca da parte radicular, MS 100% + sacarose (3%) (em 12hs/28 dias e 16hs/21 dias). Para o alongamento/enraizamento in vitro da bananeira cv. Pacovan sugere-se MS 50% de sais (macro e micronutrientes), redução ou manutenção de sacarose (1,5 ou 3%) em 16h/35 dias de cultivo.Palavra-chave: Musa spp., propagação in vitro, sistema radicular. CHANGES IN CULTURE MEDIUM, PHOTOPERIOD AND TIME OF CULTIVATION AFFECT THE IN VITRO ELONGATION AND ROOTING OF BANANA CV. PACOVAN ABSTRACT:In order to achieve high rates of survival during the acclimatization and later planting in the field, was evaluated the in vitro of banana cv. Pacovan plants under different concentrations of sucrose and MS basal salt mixture. The experiment was assembled in a DIC, in 6x2x3, six different culture media [three different MS salt mixture concentrations (100%; 50% of macronutrients; and 50% of macro/micronutrients) and two sucrose concentrations (1.5/3%)], two photoperiods (12/16 hours) and three cultivation times (21, 28 or 35 days). Each treatment was composed by 6 replicates. Plant height, number of leaves/plant, fresh and dry weight of roots and shoots, were analyzed. Satisfactory results for plant height and shoot and root fresh biomass were observed in MS with macro/micronutrients (50%) + sucrose (3%), 16 hours/35 days. The highest values of shoot dry weight were observed in MS with macro/micronutrients (50%) + sucrose (3%); the highest root dry weight was achieved with MS 100% + sucrose (3%) (12hs/28 and 16hs/21 days). The suggested medium for the in vitro elongation and rooting stage of banana cv. Pacovan is the MS with 50% of salts (macro and micronutrients), reduction or maintenance of sucrose (1.5 or 3%) in 16h/35 days of cultivation.Keywords: Musa spp., in vitro propagation, root system. DOI:

scholarly journals Selection of filamentous fungi that are resistant to the herbicides atrazine, glyphosate and pendimethalin

2021 ◽  
Vol 43 ◽  
pp. e51656
Author(s):  
Nara Priscila Barbosa Bravim ◽  
Anatércia Ferreira Alves ◽  
José Fábio França Orlanda ◽  
Patricia Barbosa Rodrigues Silva
Keyword(s):  
Filamentous Fungi ◽  
Mycelial Growth ◽  
Culture Medium ◽  
Agricultural Soils ◽  
Culture Media ◽  
Fusarium Verticillioides ◽  
Fungal Growth ◽  
Relative Growth ◽  
Maximum Inhibition ◽  
Selection Of

The objective of the present study was to isolate fungi from agricultural soils and evaluate fungal growth in culture medium contaminated with atrazine, glyphosate and pendimethalin. Filamentous fungi were isolated from agricultural soils and cultured in a modified culture medium containing 0, 10, 20, 50, and 100 μg mL-1 atrazine, glyphosate and pendimethalin for 14 days at 28°C. The fungi that presented optimal and satisfactory growth were plated in Sabouraud culture medium with 4% dextrose and containing the herbicides at concentrations of 0, 10, 20, 50, and 100 μg mL-1 for seven days at 28°C. The mean mycelial growth values were submitted to analysis of variance and the Tukey test (p < 0.05%) for comparison and relative growth determination, and maximum inhibition rates were calculated. The isolated fungi Aspergillus fumigatus, Fusarium verticillioides and Penicillium citrinum were shown to be resistant to atrazine, glyphosate and pendimethalin. F. verticillioides showed higher mean mycelial growth in the culture media contaminated with atrazine and glyphosate than the other two fungi. In the culture medium contaminated with pendimethalin, F. verticillioides, and A. fumigatus presented the highest mean mycelial growth values.

Number of Leaves and Maturity Classification in Zea mays L. 1

Crop Science ◽  
1967 ◽  
Vol 7 (5) ◽  
pp. 431-432 ◽  
Author(s):  
Sherret S. Chase ◽  
Devender K. Nanda
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Number Of Leaves

Effects of Culture Media, Exposure Time and Temperature on Near-Ultraviolet-Induced Sporulation of Alternaria alternata

1985 ◽  
Vol 48 (4) ◽  
pp. 316-319
Author(s):  
CHENG-I WEI ◽  
DIANE D. SWARTZ ◽  
JOHN A. CORNELL
Keyword(s):  
Exposure Time ◽  
Alternaria Alternata ◽  
Culture Medium ◽  
Culture Media ◽  
Media Exposure ◽  
Potato Dextrose Agar ◽  
Uv Exposure ◽  
Strain Atcc ◽  
Ultraviolet Exposure ◽  
Near Ultraviolet

Effects of culture media, near-ultraviolet exposure time, and temperature on sporulation of Alternaria alternata were investigated. Strains RL 671-2 and ATCC 36068 were cultivated on Potato Dextrose Agar (PDA), V8 Juice Agar (V8 Agar) and Mycological Agar (MA). The best culture medium for sporulation of strain RL 671-2 was PDA, followed by V8 agar, with only negligible numbers of spores appearing on MA. Near-UV exposure significantly increased sporulation in strain RL 671-2 on PDA and V8 agar. Significantly higher (P&lt;0.01) spore counts were found in PDA cultures of this strain exposed to near-UV at 35 than at 20°C. On V8 agar significantly more spores were observed at 20 than at 35°C. MA was not a satisfactory medium for sporulation of ATCC 36068. Both PDA and V8 agar equally supported sporulation for this strain (ATCC 36068) at all exposure times.

scholarly journals MÉTODOS DE DETECÇÃO DO GÊNERO Aspergillus EM SEMENTES DE MILHO (Zea mays L.) EM 270 DIAS DE ARMAZENAMENTO

2003 ◽  
Vol 4 (1) ◽  
Author(s):  
G. M. CIRIO ◽  
M. L. R. Z. C. LIMA
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Animal Health ◽  
Potato Dextrose Agar ◽  
Detection Methods ◽  
Secure Storage ◽  
Maize Seeds ◽  
Low Humidity ◽  
The Media ◽  
Seeds Germination

O gênero Aspergillus tem como característica desenvolver-se em sementes e grãos com baixa umidade causando deterioração destes durante armazenamento com efeito na germinação nas sementes e produção de micotoxinas como aflatoxina em grãos, prejudiciais a saúde do homem e dos animais. Para avaliar a eficiência de métodos de detecção para Aspergillus, verificar germinação e umidade das sementes de milho foi feita armazenagem por 270 dias. As avaliações foram ao zero, 90, 150, 210 e 270 dias após armazenamento (DAA) com testes em papel filtro (blotter) e nos meios de batata dextrose ágar ácido (BDA ácido) e ágar suco de tomate mais 6% de cloreto de sódio (AST salino). Como resultados ao zero dia de armazenamento verificou-se ausência de Aspergillus; aos 90 DAA incidências 7,2% em blotter e 1,1% em AST; aos 150 DAA incidências 5,1% em blotter; aos 210 DAA incidências 41,1% em AST, 5,1% no blotter e 3,3% no BDA e aos 270 DAA incidências de 79,7% e 66,2% em blotter e AST que não diferiram estatisticamente entre si e 21% em BDA. A germinação e umidade foram: 98% e 10,8% (dia zero); 93% e 13,4% (90 DAA); 93% e 12,2% (150 DAA); 92% e 14,2% (210 DAA) e 36 % e 12,2 % (270 DAA), respectivamente. Concluiu-se que o meio AST salino mostrou maior eficiência para detectar Aspergillus aos 210 DAA indicando proliferação do gênero durante armazenamento até 270 DAA. A germinação das sementes reduziu-se de 92% para 36% entre 210 e 270 DAA e a umidade variou entre 10,8% e 14,2%, compatível com armazenagem segura. DETECTION METHODS OF Aspergillus GENUS IN CORN SEEDS (Zea mays L.) DURING 270 DAYS STORAGE Abstract The genera Aspergillus has a characteristic to developed in seeds and grains with low humidity causing deterioration during storage with effects on the seeds germination andmicotoxins production as aflatoxin in grains that are harmful to man and animal health. To evaluated the efficiency of detection methods for Aspergillus, to confirm germination and humidity in maize seeds, they were stored for 270 days. The evaluations were at zero, 90, 150, 210 and 270 days after storage (DAS) with the blotter test and in media potato-dextrose-agar (PDA acid) and tomato juice agar plus 6% NaCl (salty TJA). As results at zero day absence of Aspergillus, at 90 DAS incidence 7,2% in blotter and 1,1% in TMA, at 150 DAS incidence 5,1% in blotter; at 210 DAS the incidence 41,1% in TMA, 5,1% in blotter e 3,3% in PDA and at 270 DAS incidence 79,7% and 66,2% in blotter and TMA that didn’t differ statistically and 21% in PDA. Were get germination and humidity 98% and 10,8% (zero day); 93% and 13,4% (90 DAS); 93% and 12,2% (150 DAS); 92% and 14,2% (210 DAS) and 36% and 12,2% (270 DAS) respectively. The conclusions were that the media salty TJA showed higher efficiency to detect Aspergillus at 210 DAS what indicated proliferation of the genera during storage till 270 DAS. The germination reduced from92% to 36% between 210 e 270 DAS and humidity remained between 10,8% and 14,2% compatible with secure storage.

Phosphite-based Products in the In vitro Colletotrichum musae Control

2019 ◽  
pp. 1-9
Author(s):  
Maria Luísa Mendes Rodrigues ◽  
Edson Hiydu Mizobutsi ◽  
Paola Junayra Lima Prates ◽  
Paula Virgínia Leite Duarte ◽  
Regina Cássia Ferreira Ribeiro ◽  
...  
Keyword(s):  
Mycelial Growth ◽  
Culture Medium ◽  
Culture Media ◽  
Petri Dish ◽  
State University ◽  
Randomized Design ◽  
Colletotrichum Musae ◽  
Study Laboratory ◽  
Vitro Effect

Aims: The aim of this study was to evaluate the in vitro effect of different phosphite formulations and concentrations on the development of Colletotrichum musae. Sample: to evaluate the inhibition of germination, mycelial growth and sporulation of Colletotrichum musae. Study Design:  Treatments were conducted in a completely randomized design, with 4 replicates, each replicate consisting of 1 Petri dish. Place and Duration of Study:  Laboratory of Post-Harvest Pathology, State University of Montes Claros, between March and October 2017. Methodology: Three different phosphite formulations were used: FCu1 (4% Cu + 20% P2O5), FCu2 (4% Cu + 22% P2O5) at concentrations of 0.5;1.0; 1.5 and 2.0 mL L-1 and FK (42% P2O5 + 27.7% K2O) at concentrations of 0.5; 1.0; 1.5 and 2.0 mg.L-1. Products were incorporated into the respective culture media. Culture medium alone and culture medium + imazalil were used as controls. Petri dishes were housed in BOD chamber at 25°C under a 12 hours photoperiod. Results: Results were submitted to analysis of variance and regression, and means were compared by the Tukey test (P <0.05). Control was compared to the other treatments by the Dunnet's test (P <0.05). Among the tested phosphite formulations, copper and potassium phosphites were found to reduce the mycelial growth of Colletotrichum musae. FCu2 presents a fungicide-like effect from the concentration of 0.5 m.L-1 in the control of conidia production. As for the FCu1, a fungicide-like effect was observed in the control of germination from the concentration of 1.5 mL.L-1. Conclusion: A significant fungistatic effect was observed between the concentrations of the products in the mycelial growth, sporulation and germination obtaining control of up to 100% of the development of C. musae. Copper phosphites were as effective as fungicide in inhibiting fungal development.

scholarly journals PROSPECÇÃO E AVALIAÇÃO DO POTENCIAL BIOTECNOLÓGICO DE LINHAGENS COMERCIAIS DE SHIITAKE

2021 ◽  
Vol 35 (4) ◽  
pp. 640-649
Author(s):  
Dalvan Pereira Abilio ◽  
Olívia Gomes Martins ◽  
Giovana Silva ◽  
Meire Andrade
Keyword(s):  
Wheat Bran ◽  
Radial Growth ◽  
Mycelial Growth ◽  
Culture Medium ◽  
Lentinula Edodes ◽  
Culture Media ◽  
Biotechnological Potential ◽  
E Mail ◽  
Eucalyptus Sawdust

PROSPECÇÃO E AVALIAÇÃO DO POTENCIAL BIOTECNOLÓGICO DE LINHAGENS COMERCIAIS DE SHIITAKE   DALVAN PEREIRA ABÍLIO1, OLÍVIA GOMES MARTINS2, GIOVANA CRISTINA PINTO ALVES DA SILVA3, MEIRE CRISTINA NOGUEIRA DE ANDRADE4   1 Graduando em Ciências Biológicas, Centro Universitário Sagrado Coração – Unisagrado, R. Irmã Arminda, 10-50 - Jardim Brasil, 17011-160 Bauru, SP, Brasil. E-mail: [email protected] 2 Doutoranda em Agronomia – Energia na Agricultura, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista "Julio de Mesquita Filho" (UNESP), Av. Universitária, 3780 - Altos do Paraíso, 18610-034, Botucatu, SP, Brasil. E-mail: [email protected] 3 Mestre em Agronomia – Energia na Agricultura, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista "Julio de Mesquita Filho" (UNESP), Av. Universitária, 3780 - Altos do Paraíso, 18610-034, Botucatu, SP, Brasil. E-mail: [email protected] 4 Docente da Faculdade Gran Tietê, Av. 15 de Novembro, 125 - Centro, 17340-000, Barra Bonita, SP, Brasil. E-mail: [email protected]   RESUMO: O objetivo deste trabalho foi avaliar e comparar o crescimento micelial in vitro de quatro linhagens comerciais de Lentinula edodes (LE-241, LE-242, LE-243 e LE-244) em meio de cultura à base de serragem de eucalipto, suplementado com bagaço de malte ou farelo de trigo. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 2x4 (substratos x linhgens), totalizando oito tratamentos, cada um com cinco repetições. Os substratos foram preparados com 20% de suplementação, acrescidos de calcário (tamponante), umidificados e esterilizados. Os meios de cultura foram preparados a partir da infusão dos substratos, filtração, adição de ágar e esterilização. As placas foram inoculadas com as linhagens e o crescimento radial do micélio na superfície do meio de cultura foi mensurado com paquímetro. O menor desenvolvimento micelial ocorreu com a linhagem LE-241 e com a linhagem LE-243 no substrato suplementado com farelo de trigo. Os maiores desenvolvimentos foram obtidos com as linhagens LE-242 e LE-244 no substrado suplementado com bagaço de malte. O desenvolvimento de todas as linhagens foi maior no substrato suplementado com bagaço de malte. Sendo assim, a utilização do bagaço de malte para o crescimento micelial é uma alternativa viável à suplementação tradicional com farelo de trigo.   Palavras-chave: fungos, cogumelo, Lentinula edodes, micélio, bagaço de malte.   PROSPECTING AND EVALUATING THE BIOTECHNOLOGICAL POTENTIAL OF SHIITAKE COMMERCIAL STRAINS   ABSTRACT: This study aimed to evaluate and compare the in vitro mycelial growth of four commercial Lentinula edodes strains (LE-241, LE-242, LE-243 and LE-244) in a culture medium based on eucalyptus sawdust, supplemented with malt bagasse or wheat bran. The experimental design was completely randomized, in a 2x4 factorial scheme (substrates x strains), totaling eight treatments, each with five repetitions. The substrates were prepared with 20% supplementation, added with lime (buffer), humidified and sterilized. The culture media were prepared from the infusion of substrates, filtration, addition of agar and sterilization. The plates were inoculated with the strains and the radial growth of the mycelium on the surface of the culture medium was measured using a caliper. The smallest mycelial growth occurred with the LE-241 strain and with the LE-243 strain on the substrate supplemented with wheat bran. The greatest growths were obtained with the LE-242 and LE-244 strains in the substrate supplemented with malt bagasse. The development of all strains was greater in the substrate supplemented with malt bagasse. Therefore, the use of malt bagasse for mycelial growth is a viable alternative to traditional wheat bran supplementation.   Keywords: fungi, mushroom, Lentinula edodes, mycelium, malt bagasse.

Sign in / Sign up

Export Citation Format

Share Document