Antagonistic Bacteria Bacillus subtilis Formulation as Biopesticide to Control Corn Downy Mildew caused by Peronosclerospora philippinensis

2021 ◽  
Vol 11 (6) ◽  
pp. 2148
Author(s):  
Nurasiah Djaenuddin ◽  
Septian Hary Kalqutny ◽  
Amran Muis ◽  
Muhammad Azrai
Keyword(s):  
Bacillus Subtilis ◽  
Downy Mildew ◽  
Antagonistic Bacteria

scholarly journals Kemampuan Bacillus subtilis dan Lysinibacillus sp. dalam Silika Nano dan Serat Karbon untuk Menginduksi Ketahanan Bawang Merah terhadap Penyakit Bercak Ungu (Alternaria porri (Ell.) Cif)

Agrikultura ◽  
2019 ◽  
Vol 30 (1) ◽  
pp. 8
Author(s):  
Hersanti Hersanti ◽  
Sudarjat Sudarjat ◽  
Andina Damayanti
Keyword(s):  
Bacillus Subtilis ◽  
Carbon Fiber ◽  
Block Design ◽  
Antagonistic Bacteria ◽  
Nano Silica ◽  
Green House ◽  
Randomized Block Design ◽  
Plant Pest ◽  
Alternaria Porri ◽  
Induce Resistance

ABSTRACTThe ability of Bacillus subtilis and Lysinibacillus sp. singly or mixed, with carbon fiber and nano silica to induce resistance of shallot to purple blotchPurple blotch disease caused by Alternaria porri is one of the major disease on shallot. One of the methods that can be applied to control the disease is the use of antagonistic bacteria. Antagonistic bacteria can be used as a resistance inducer to suppress pathogen development. In this study, Bacillus subtilis and Lysinibacillus sp. were formulated in carbon fiber as a carrier and nano silica 3% as a complementary. This study was conducted to determine the ability of Bacillus subtilis and Lysinibacillus sp. singly or mixed, with carbon fiber and nano silica to induce resistance of shallot to purple blotch. The experiment was conducted at the Laboratory of Phytopathology, Departement of Plant Pest and Diseases and Ciparanje Green House, Faculty of Agriculture, Universitas Padjadjaran from December 2017 until July 2018. Suspension of Bacillus subtilis and Lysinibacillus sp. singly or mixed were formulated in carbon fiber 80 Mesh and 3% nano silica. The experiment used Randomized Block Design consisted of 8 treatments with 3 replications. Each replication consisted of 5 plants. The results showed that the mixture of Bacillus subtilis and Lysinibacillus sp. in 3% silica nano and carbon fiber was the ablest treatment to increase the resistance of shallot to purple blotch by 71,2%.Keywords: Antagonistic bacteria, BiocontrolABSTRAKPenyakit bercak ungu yang disebabkan oleh Alternaria porri merupakan salah satu penyakit utama pada bawang merah. Salah satu metode yang dapat digunakan untuk pengendalian yaitu menggunakan bakteri antagonis. Bakteri antagonis dapat digunakan sebagai penginduksi ketahanantanaman untuk menekan perkembangan penyakit. Pada penelitian ini, Bacillus subtilis dan Lysinibacillus sp. diformulasikan dalam serat karbon sebagai bahan pembawa dan silika nano 3% sebagai bahan pelengkap. Percobaan ini dilakukan untuk mengetahui kemampuan bakteri Bacillus subtilis dan Lysinibacillus sp. secara tunggal maupun campuran dalam serat karbon dan silika nano3% untuk menginduksi ketahanan bawang merah terhadap penyakit bercak ungu. Percobaan ini dilaksanakan di Laboratorium Fitopatologi, Departemen Hama dan Penyakit Tumbuhan dan Rumah Kaca, Ciparanje, Fakultas Pertanian, Universitas Padjadjaran mulai dari bulan Desember 2017 hingga Juli 2018. Suspensi Bacillus subtilis dan Lysinibacillus sp. secara tunggal maupun campuran diformulasikan pada serat karbon dan silika nano 3%. Percobaan menggunakan rancangan acak kelompok yang terdiri dari 8 perlakuan dengan 3 ulangan. Setiap ulangan terdiri dari 5 tanaman. Dari hasil percobaan diketahui bahwa campuran B. subtilis dan Lysinibacillus sp. dalam silika nano 3% serta serat karbon mampu meningkatkan ketahanan bawang merah terhadap penyakit bercak ungu dengan persentase hambatan sebesar 71,2%.Kata Kunci: Bakteri antagonis, Biokontrol

scholarly journals The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1) and Bacillus subtilis (UB_Bs1) on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae)

2016 ◽  
Vol 8 (3) ◽  
pp. 286
Author(s):  
Presti Mardiyani Purwaningtyas ◽  
Bambang Tri Rahardjo ◽  
Hagus Tarno
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Biology Education ◽  
Antagonistic Bacteria ◽  
Median Lethal Concentration ◽  
Pratylenchus Coffeae ◽  
Lethal Time ◽  
Bacterial Treatment ◽  
Coffee Plants ◽  
Nematode Mortality

<p>Parasitic Root-Lession nematode of <em>Pratylenchus coffeae</em> can reduce the Indonesian coffee plants productivity. Several studies reported that <em>Pseudomonas fluorescens </em>and <em>Bacillus subtilis </em>endophytic bacteria were antagonistic bacteria to nematode. The objective of this research was to reveal the effectiveness of bacterial colonies density of <em>P. fluorescens </em>(UB_Pf1), <em>B.subtilis </em>(UB BS1), and a combination of both bacteria on nematode mortality using median lethal concentration (LC<sub>50</sub>) and median lethal time 50 (LT<sub>50</sub>). The densities of bacteria used in this study were 10<sup>7</sup>, 10<sup>9</sup>, 10<sup>11 </sup>and 10<sup>13 </sup>cfu/ml. 35 testing nematodes were used and the mortality was counted at 6, 12, 24, 36, and 48 hours after treatments. The results showed that LC<sub>50</sub> values of <em>P. fluorescens </em>was (UB_Pf1) was 4,3x10<sup>8 </sup>cfu/ml, LC<sub>50 </sub><em>B. subtilis </em>(UB_Bs1) was 1,9x10<sup>9</sup>cfu/ ml, and LC<sub>50 </sub>combination of both bacteria was, 8x10<sup>7 </sup>cfu/ml. It implies that the application of the combination of both bacteria are more pathogenic than single bacterial treatment. The results also showed that the highest LT<sub>50 </sub>value was 13.21  hours combination of bacterial colonies with a density of 10<sup>13 </sup>cfu/ml and the lowest LT<sub>50 </sub>value was 52.00 hours on <em>P. fluorescens </em>(UB_Pf1) treatment with colonies density of 10<sup>7 </sup>cfu/ml.</p><p><strong>How to Cite</strong></p><p>Purwaningtyas, P. M., Rahardjo, B. T., &amp; Tarno, H. (2016). The Effect of Bacteria Colony <em>Pseudomonas fluorescens</em> (UB_Pf1) and <em>Bacillus subtilis</em> (UB_Bs1) on the Mortality of <em>Pratylenchus coffeae</em> (Tylenchida: Pratylenchidae). <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(3), 286-293.</p><p> </p>

scholarly journals SCREEN HOUSE TEST OF EIGHT BIOPESTICIDE FORMULATION BACILLUS SUBTILIS AGAINST DOWNY MILDEW, PERONOSCLEROSPORA PHILIPINENSIS, ON CORN PLANT

2018 ◽  
Vol 18 (1) ◽  
pp. 51
Author(s):  
Nurasiah Djaenuddin ◽  
Amran Muis ◽  
Nurnina Nonci
Keyword(s):  
Bacillus Subtilis ◽  
Downy Mildew ◽  
Block Design ◽  
Dry Weight ◽  
Disease Suppression ◽  
Water Control ◽  
Corn Plant ◽  
Maize Production ◽  
Randomized Block Design ◽  
Screen House

Screen House Test of Eight Biopesticide Formulation Bacillus subtilis against Downy Mildew, Peronosclerospora philipinensis, on Corn Plant. Corn downy mildew (DM) caused by Peronosclerospora spp. is a major constraint to the decline in maize production because it can cause yield loss up to 100%. So far, DM was successfully controlled with metalxyl fungicide. But lately, the effectiveness of this fungicide has begun to decline, so there is a need for an appropriate and environmentally friendly control alternative, one of which is the use of biological control agents. The aim of this study was to know the influence of eight types of biopesticide formulation of Bacillus subtilis in controlling DM of corn plant. The study was conducted at the screenhouse of the Indonesian Cereals Research Institute at Maros from November to December 2016. The treatments were arranged in a randomized block design with nine treatments and three replications. The treatment consisted of seed treatment with eight types of B. subtilis formulations (TM4, TM3, BNt4, BNt5, BNt6, BNt8, BJ6, and TLB1) and one treatment with sterile water (control). The variety used was Anoman which is known to be highly susceptible to DM. The variables observed included number of germination, the incidence and incubation period of disease, plant height, and dry weight of the plant. The results showed that the eight B. subtilis formulations had the potential to suppress DM in corn. The symptom of DM began to be seen at 18 days after planting (DAP) on the treatment of BNt8, while the controls have been seen at 12 HST. B. subtilis TM formulation has the highest potential to suppress DM with the percentage of disease suppression reaching 63.1%.

Extracellular protease from Bacillus subtilis B315 with antagonistic activity against bacterial wilt pathogen (Ralstonia solanacearum) of chili

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Nur Prihatiningsih ◽  
ARI ASNANI ◽  
HERU ADI DJATMIKO
Keyword(s):  
Bacillus Subtilis ◽  
Crude Extract ◽  
Incubation Time ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Antagonistic Activity ◽  
Extracellular Protease ◽  
Antagonistic Bacteria ◽  
Clear Zone

Abstract. Prihatiningsih N, Asnani A, Djatmiko HA. 2021. Extracellular protease from Bacillus subtilis B315 with antagonistic activity against bacterial wilt pathogen (Ralstonia solanacearum) of chili.  Biodiversitas 22: 1291-1295. Antagonistic bacteria isolated from a healthy potato are potential biocontrol agents against a number of bacterial pathogens. The capability of antagonistic bacteria to produce extracellular protease could be considered as an indicator of their antagonistic capacity. The objective of this research was to study the optimum activity of protease produced by Bacillus subtilis B315 to control Ralstonia solanacearum causing wilt of chili. In this research were optimum incubation time, pH and crude extract protease concentration of B. subtilis B315 examined for maximum production of extracellular protease. The results showed that B. subtilis B315 was capable of producing protease at optimum incubation time of 30 min, pH 9, and crude extract protease concentration 0.50% with an activity of 1.3185 U/mL. Protease-producing capability of B. subtilis B315 was shown by the formation of a clear zone around the colony of B. subtilis B315 grown on skim-milk agar medium. B. subtilis B315 was capable of inhibiting the growth of R. solanacearum in vitro as indicated by 32 mm clear zone. Furthermore, in chili application of B subtilis showed that this bacteria was capable of controlling chili bacterial wilt caused by R. solanacearum with effectiveness of 60.89%. The prospective of B. subtilis B315 could be formulated as biopesticide.

scholarly journals SCREEN HOUSE TEST OF EIGHT BIOPESTICIDE FORMULATION BACILLUS SUBTILIS AGAINST DOWNY MILDEW, PERONOSCLEROSPORA PHILIPINENSIS, ON CORN PLANT

2018 ◽  
Vol 18 (1) ◽  
pp. 51
Author(s):  
Nurasiah Djaenuddin ◽  
Amran Muis ◽  
Nurnina Nonci
Keyword(s):  
Bacillus Subtilis ◽  
Downy Mildew ◽  
Block Design ◽  
Dry Weight ◽  
Disease Suppression ◽  
Water Control ◽  
Corn Plant ◽  
Maize Production ◽  
Randomized Block Design ◽  
Screen House

Screen House Test of Eight Biopesticide Formulation Bacillus subtilis against Downy Mildew, Peronosclerospora philipinensis, on Corn Plant. Corn downy mildew (DM) caused by Peronosclerospora spp. is a major constraint to the decline in maize production because it can cause yield loss up to 100%. So far, DM was successfully controlled with metalxyl fungicide. But lately, the effectiveness of this fungicide has begun to decline, so there is a need for an appropriate and environmentally friendly control alternative, one of which is the use of biological control agents. The aim of this study was to know the influence of eight types of biopesticide formulation of Bacillus subtilis in controlling DM of corn plant. The study was conducted at the screenhouse of the Indonesian Cereals Research Institute at Maros from November to December 2016. The treatments were arranged in a randomized block design with nine treatments and three replications. The treatment consisted of seed treatment with eight types of B. subtilis formulations (TM4, TM3, BNt4, BNt5, BNt6, BNt8, BJ6, and TLB1) and one treatment with sterile water (control). The variety used was Anoman which is known to be highly susceptible to DM. The variables observed included number of germination, the incidence and incubation period of disease, plant height, and dry weight of the plant. The results showed that the eight B. subtilis formulations had the potential to suppress DM in corn. The symptom of DM began to be seen at 18 days after planting (DAP) on the treatment of BNt8, while the controls have been seen at 12 HST. B. subtilis TM formulation has the highest potential to suppress DM with the percentage of disease suppression reaching 63.1%.

scholarly journals Consortia of Indigenous Rhizobacteria to Control Bacterial Wilt in Ginger

2020 ◽  
Vol 7 (2) ◽  
pp. 75-83
Author(s):  
Naziha Diyanatur Rosiyah ◽  
Ayu Wike Widiasari ◽  
Achmad Roekhan ◽  
Restu Rizkyta Kusuma
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Thuringiensis ◽  
Bacterial Wilt ◽  
Scientific Literature ◽  
Disease Incidence ◽  
Zingiber Officinale ◽  
Wilt Disease ◽  
Antagonistic Bacteria ◽  
Bacterial Wilt Disease ◽  
Inhibiting Factors

Ginger (Zingiber officinale Rosc.) is a type of rhizome plant that has high potential to be developed in Indonesia as a medicinal plant or spice. Inhibiting factors in ginger production is Ralstonia solanacearum which causes bacterial wilt disease. Rhizobacteria is a potential biological agent to control the disease. It is necessary to review from various study on rhizobacteria both the use of antagonistic bacteria singly or in a consortia. Aimed to analyze various sources of scientific literature regarding the potential of antagonistic bacteria singly or consortia compared to synthetic bactericides in controlling of R. solanacearum. The results showed that three recommended antagonist bacteria were found, namely Pseudomonas fluorescens, Bacillus subtilis, and Bacillus thuringiensis. which has the potential to control bacterial wilt disease. The rhizobacterial consortia are more effective as a sustainable, safe and environmentally friendly control on bacterial wilt disease with a percentage of disease incidence 10%. In addition, the rhizobacterial consortia are three and six times more effective than the single antagonistic bacteria and bactericides respectively.

scholarly journals Kemampuan Bacillus subtilis dan Lysinibacillus sp. dalam Silika Nano dan Serat Karbon untuk Menginduksi Ketahanan Bawang Merah terhadap Penyakit Bercak Ungu (Alternaria porri (Ell.) Cif)

Agrikultura ◽  
2019 ◽  
Vol 30 (1) ◽  
pp. 8
Author(s):  
Hersanti Hersanti ◽  
Sudarjat Sudarjat ◽  
Andina Damayanti
Keyword(s):  
Bacillus Subtilis ◽  
Carbon Fiber ◽  
Block Design ◽  
Antagonistic Bacteria ◽  
Nano Silica ◽  
Green House ◽  
Randomized Block Design ◽  
Plant Pest ◽  
Alternaria Porri ◽  
Induce Resistance

ABSTRACTThe ability of Bacillus subtilis and Lysinibacillus sp. singly or mixed, with carbon fiber and nano silica to induce resistance of shallot to purple blotchPurple blotch disease caused by Alternaria porri is one of the major disease on shallot. One of the methods that can be applied to control the disease is the use of antagonistic bacteria. Antagonistic bacteria can be used as a resistance inducer to suppress pathogen development. In this study, Bacillus subtilis and Lysinibacillus sp. were formulated in carbon fiber as a carrier and nano silica 3% as a complementary. This study was conducted to determine the ability of Bacillus subtilis and Lysinibacillus sp. singly or mixed, with carbon fiber and nano silica to induce resistance of shallot to purple blotch. The experiment was conducted at the Laboratory of Phytopathology, Departement of Plant Pest and Diseases and Ciparanje Green House, Faculty of Agriculture, Universitas Padjadjaran from December 2017 until July 2018. Suspension of Bacillus subtilis and Lysinibacillus sp. singly or mixed were formulated in carbon fiber 80 Mesh and 3% nano silica. The experiment used Randomized Block Design consisted of 8 treatments with 3 replications. Each replication consisted of 5 plants. The results showed that the mixture of Bacillus subtilis and Lysinibacillus sp. in 3% silica nano and carbon fiber was the ablest treatment to increase the resistance of shallot to purple blotch by 71,2%.Keywords: Antagonistic bacteria, BiocontrolABSTRAKPenyakit bercak ungu yang disebabkan oleh Alternaria porri merupakan salah satu penyakit utama pada bawang merah. Salah satu metode yang dapat digunakan untuk pengendalian yaitu menggunakan bakteri antagonis. Bakteri antagonis dapat digunakan sebagai penginduksi ketahanan tanaman untuk menekan perkembangan penyakit. Pada penelitian ini, Bacillus subtilis dan Lysinibacillus sp. diformulasikan dalam serat karbon sebagai bahan pembawa dan silika nano 3% sebagai bahan pelengkap. Percobaan ini dilakukan untuk mengetahui kemampuan bakteri Bacillus subtilis dan Lysinibacillus sp. secara tunggal maupun campuran dalam serat karbon dan silika nano 3% untuk menginduksi ketahanan bawang merah terhadap penyakit bercak ungu. Percobaan ini dilaksanakan di Laboratorium Fitopatologi, Departemen Hama dan Penyakit Tumbuhan dan Rumah Kaca, Ciparanje, Fakultas Pertanian, Universitas Padjadjaran mulai dari bulan Desember 2017 hingga Juli 2018. Suspensi Bacillus subtilis dan Lysinibacillus sp. secara tunggal maupun campuran diformulasikan pada serat karbon dan silika nano 3%. Percobaan menggunakan rancangan acak kelompok yang terdiri dari 8 perlakuan dengan 3 ulangan. Setiap ulangan terdiri dari 5 tanaman. Dari hasil percobaan diketahui bahwa campuran B. subtilis dan Lysinibacillus sp. dalam silika nano 3% serta serat karbon mampu meningkatkan ketahanan bawang merah terhadap penyakit bercak ungu dengan persentase hambatan sebesar 71,2%.Kata Kunci: Bakteri antagonis, Biokontrol

scholarly journals Identification and Evaluation of Strain B37 ofBacillus subtilisAntagonistic to Sapstain Fungi on Poplar Wood

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
XiaoHua Zhang ◽  
GuiHua Zhao ◽  
DeWei Li ◽  
ShunPeng Li ◽  
Qing Hong
Keyword(s):  
Bacillus Subtilis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Morphological Characteristics ◽  
Wood Chips ◽  
Rrna Gene ◽  
Antagonistic Bacteria ◽  
Poplar Wood ◽  
The 16S Rrna Gene ◽  
Biocontrol Bacterium

Devaluation of poplar products by sapstain accounts for huge and unpredictable losses each year in China. We had isolated four poplar sapstain fungi,Ceratocystis adiposaHz91,Lasiodiplodia theobromaeYM0737,L. theobromaeFx46, andFusariumsp. YM05, from five poplar varieties and 13 antagonistic bacteria from nine diverse varieties. After being experimented with agar plates, wood chips, and enzyme activities, strain B37 was identified as the best poplar sapstain biocontrol bacterium. The strain B37 was identified asBacillus subtilisusing sequences of the 16S rRNA gene, physiological biochemical, and morphological characteristics.

scholarly journals Combination of Different Antagonistic Bacteria to Control of Potato Blackleg Disease Caused by Pectobacterium atrosepticum under Greenhouse and Field Conditions

2016 ◽  
Vol 5 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Hoda H. El-Hendawy ◽  
Kamal A. M. Abo-Elyousr
Keyword(s):  
Bacillus Subtilis ◽  
Indole Acetic Acid ◽  
Field Conditions ◽  
Antagonistic Bacteria ◽  
Bacterial Isolates ◽  
Blackleg Disease ◽  
Pectobacterium Atrosepticum ◽  
Potato Plants ◽  
Black Leg

Three different antagonistic bacterial isolates, Pseudomonas fluoresces (Pf2), Bacillus subtilis(Bs3) and Rahnella acquatilis (Ra39) restricted the growth of Pectobacterium atrosepticum, the causal agent of black leg disease of potato, in vitro.   Under greenhouse and field conditions, potato plants pre-treated with the three antagonistic bacterial isolates, individually or in combination, showed reduced disease severity relative to non-treated control plants. All isolates produced siderophores in different degrees but did not produce indole acetic acid (IAA) or hydrogen cyanide (HCN).The obtained results indicated that combination of Pseudomonas fluoresces (Pf2), Bacillus subtilis (Bs3) and Rahnella acquatilis (Ra39) is beneficial in controlling black leg disease of potato caused by pectobacterium atrosepticum.

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