Differentiation of Cholera Vibryo Strains from Microorganisms - Representatives of the Genus Aeromonas Using the Nutritional Medium "Arginine-Iron-Sugar Agar"

2021 ◽  
pp. 119-125
Author(s):  
Alexey B. Mazrukho ◽  
Denis I. Kaminsky ◽  
Vladimir V. Lobanov ◽  
Dmitry V. Sokov ◽  
Irina V. Arkhangelskaya ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Nutrient Medium ◽  
Iron Sucrose ◽  
Promising Direction ◽  
Nutritional Medium

The study of the possibility of using the developed nutrient medium "Arginine-iron-sucrose agar" for the differentiation of Vibrio cholerae strains of various origins from microorganisms of the genus Aeromonas was carried out. The results obtained allow us to consider the use of this medium as a promising direction for solving the problem of differentiation of V. cholerae strains from closely related aeromonads at the stages of selecting suspicious colonies from alkaline agar and differential agarized media of the TCBS type and selecting cultures for further identification. The developed environment can be recommended for bacteriological laboratories at the territorial level conducting monitoring studies for cholera.

scholarly journals Sorbents of Phenols As a Components of the Nutritional Medium in Microclonal Reproduction of Plants

2021 ◽  
pp. 39-48
Author(s):  
Anna Pugacheva ◽  
◽  
Kristina Bikmetova ◽  
Yuliya Smirnova ◽  
◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Silicon Dioxide ◽  
Nutrient Medium ◽  
Normal Development ◽  
Expanded Graphite ◽  
Mercury Chloride ◽  
Negative Effects ◽  
Carbon Compounds ◽  
Nutritional Medium

In the process of microclonal reproduction, plants secrete various substances into the nutrient medium, for example, phenolic compounds, which act as inhibitors of growth processes and, accordingly, prevent the normal development of explants in vitro. Plant tissues are treated with stabilizing substances, and various sorbents are also used as components of the nutrient medium to neutralize the negative effects of phenols. This paper presents an overview of the approved methods for solving the problem of sorption of phenolic compounds during microclonal propagation of plants. Various studies are considering the addition of certain components to the nutrient medium that prevent the release of harmful growth-inhibiting substances. Most often, various carbon compounds, such as activated carbon, are used as an adsorbent. The authors, based on the analysis of domestic and foreign literature on this topic, conclude that the most effective and frequently used are carbon compounds and the polymer polyvinyl pyrrolidone, less common is the use of the following inhibitory substances: ascorbic and citric acids, silver nitrate and mercury chloride. According to the results of the conducted analytical studies, the prospects of using such substances as thermally expanded graphite (TEG) and colloidal silicon dioxide as sorbents in the composition of the drug “Polysorb” were revealed. Due to the inhomogeneous porous structure, including both micropores and meso- or macropores, TEG is able to adsorb pollutants both from the solution and from the water surface, which makes it a potential sorbent for phenolic compounds. The effect of silicon dioxide, in amorphous form, on plants in vitro has already been successfully tested by some researchers, which indicates the prospects of its study.

scholarly journals Ecological safety and prospects of development of low-waste technologies in the biotechnology industry

2021 ◽  
Vol 21 (3) ◽  
pp. 317-323
Author(s):  
Vladislav R. Vol’nikov ◽  
◽  
Alexander Yu. Ul’yanov ◽  
Ruslan R. Salikhov ◽  
Oksana S. Durakova ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Nutrient Medium ◽  
Environmental Safety ◽  
Biologically Active ◽  
Production Waste ◽  
Cholera Vaccine ◽  
Waste Products ◽  
Horse Blood Serum ◽  
Industrial Strains ◽  
Treatment Facilities

Environmental pollution with industrial waste is an urgent problem today. A special place in the list of pollutants belongs to waste from biotechnological enterprises and industries, whose activities are related to the production of various drugs. Russian Research Anti-Plague Institute «Microbe» is the only manufacturer of unique immunobiological drugs in the Russian Federation – bivalent chemical cholera vaccine and rabies immunoglobulin from horse blood serum (AIG). At present, the institute actively uses fibrin as a basis for nutrient media – a waste in the production of AIG; a technology for the regeneration of alcohol waste has been developed; biologically active substances were obtained from the production waste of specific components of the cholera vaccine. The aim of the work was to assess the prospects of using waste products from the production of specific components of cholera vaccine (cholerogen-toxoid – X-AT, and O-antigen – O-AG) – formalized detoxified microbial-free filtrate (FMF), as a nutrient medium for the cultivation of industrial strains of microorganisms. It has been shown that the best methods for reducing formalin concentration are autoclaving and chemical neutralization with aqueous ammonia. During low-volume cultivation of Vibrio cholerae 569B and V. cholerae M-41 strains on all variants of experimental media based on PBP, an increase in biomass was noted. The production of Vibrio cholerae antigens at a level comparable to that of growing on a control nutrient medium was recorded in a medium variant based on O-AG production waste. The use of FMF as a nutrient medium in the future will reduce the volume of waste generated and reduce the load on the treatment facilities of the Institute, which will increase the environmental safety of production.

scholarly journals VIBRIO CHOLERAE EL TOR ANTIBIOTIC RESISTANCE GATHERED FROM ENVIRONMENTAL OBJECTS ON THE TERRITORY OF THE RUSSIAN FEDERATION

2019 ◽  
pp. 61-64
Author(s):  
N.A. Selyanskaya ◽  
L.A. Egiazaryan ◽  
A.V. Trishina ◽  
E.A. Bereznyak ◽  
I.R. Simonova
Keyword(s):  
Antibiotic Resistance ◽  
Vibrio Cholerae ◽  
Russian Federation ◽  
Nutrient Medium ◽  
Quinolone Resistance ◽  
Antibacterial Drugs ◽  
Environmental Objects ◽  
The Russian Federation ◽  
El Tor ◽  
Serial Dilutions

The paper presents the analysis of Vibrio cholerae El Tor strains antibiotic resistance gathered from environmental objects on the territory of the Russian Federation during 2007–2016. We determined the sensitivity of 268 strains of V. cholerae El Tor to 12 antibacterial drugs by the method of serial dilutions in a dense nutrient medium. The strains showed resistance to 1–7 antibacterial drugs. The emergence of quinolone resistance in polyresistant strains of V. cholerae E.

Overcoming stereotypes of renewable energy

2019 ◽  
pp. 124-136
Author(s):  
Victor D. Gazman
Keyword(s):  
Renewable Energy ◽  
Empirical Analysis ◽  
Energy Generation ◽  
New Paradigm ◽  
Financial Instrument ◽  
Promising Direction ◽  
Investment Projects ◽  
Power Stations ◽  
New Generation ◽  
The Impact

The article considers prerequisites for the formation of a new paradigm in the energy sector. The factors that may affect the imminent change of leadership among the energy generation are analyzed. The variability of the projects of creation and functioning of power stations is examined. The focus is made on problematic aspects of the new generation, especially, storage and supply of energy, achieving a system of parity that ensures balance in pricing generations. The author substantiates the principles of forming system of parities arising when comparing traditional and new generations. The article presents the results of an empirical analysis of the 215 projects for the construction of facilities for renewable energy. The significance and direction of the impact of these factors on the growth in investment volumes of transactions are determined. The author considers leasing as an effective financial instrument for overcoming stereotypes of renewable energy and as a promising direction for accelerated implementation of investment projects.

In vivo Production of Soluble Inorganic Pyrophosphatases in Two Strains of Vibrio cholerae

1970 ◽  
Vol 24 (1) ◽  
pp. 38-41
Author(s):  
Taslima Taher Lina ◽  
Mohammad Ilias
Keyword(s):  
Vibrio Cholerae ◽  
Specific Activity ◽  
Experimental Conditions ◽  
Environmental Strain ◽  
Cell Extracts ◽  
Atcc Strain ◽  
The Family ◽  
Effects Of Ph ◽  
Vibrio Cholerae O1

The in vivo production of soluble inorganic pyrophosphatases (PPases) was investigated in two strains, namely, Vibrio cholerae EM 004 (environmental strain) and Vibrio cholerae O1 757 (ATCC strain). V. cholerae is known to contain both family I and family II PPase coding sequences. The production of family I and family II PPases were determined by measuring the enzyme activity in cell extracts. The effects of pH, temperature, salinity of the growth medium on the production of soluble PPases were studied. In case of family I PPase, V. cholerae EM 004 gave the highest specific activity at pH 9.0, with 2% NaCl + 0.011% NaF and at 37°C. The strain V. cholerae O1 757 gave the highest specific activity at pH 9.0, with media containing 0% NaCl and at 37°C. On the other hand, under all the conditions family II PPase did not give any significant specific activity, suggesting that the family II PPase was not produced in vivo in either strains of V. cholerae under different experimental conditions. Keywords: Vibrio cholerae, Pyrophosphatases (PPases), Specific activityDOI: http://dx.doi.org/10.3329/bjm.v24i1.1235 Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 38-41

ВПЛИВ ДЖЕРЕЛ АЗОТНОГО ЖИВЛЕННЯ НА СИНТЕЗ КАРОТИНОЇДІВ ДЕЯКИМИ ШТАМАМИ БАЗИДІОМІЦЕТІВ

2014 ◽  
Vol 4 (01) ◽  
pp. 22
Author(s):  
A. K. Veligodska ◽  
O. V. Fedotov ◽  
A. S. Petreeva
Keyword(s):  
Culture Filtrate ◽  
Nutrient Medium ◽  
Culture Fluid ◽  
Carotenoid Content ◽  
Nitrogen Compounds ◽  
Total Carotenoid ◽  
Carotenoid Accumulation ◽  
Total Carotenoid Content ◽  
Peptone Medium ◽  
Acetone Extracts

<p>The influence of certain nitrogen compounds - components of glucose-peptone medium (GPM) on the accumulation of carotenoids by some strains was investigated by surface cultivating basidiomycetes. The total carotenoid content was set in acetone extracts of mycological material spectrophotometrically and calculated using the Vetshteyn formula.</p> <p>As the nitrogen-containing components used GPM with 9 compounds, such as peptone, DL-valine, L-asparagine, DL-serine, DL-tyrosine, L-proline, L-alanine, urea, NaNO<sub>3</sub>. The effect on the accumulation of specific compounds both in the mycelium and in the culture fluid of carotenoids by culturing certain strains of Basidiomycetes was identified.</p> <p>Adding to standard glucose-peptone medium peptone at 5 g/l causes an increase of carotenoid accumulation by strain <em>L. sulphureus</em> Ls-08, and in a concentration of 4 g/l by strains of <em>F. hepatica </em>Fh-18 and <em>F. fomentarius</em> Ff-1201.</p> <p>In order to increase the accumulation of carotenoids in the mycelium  we suggested to make a standard glucose-peptone medium with proline or valine for cultivating of <em>L. sulphureus</em> Ls- 08 strain; alanine for <em>F. fomentarius</em> Ff-1201 strain; proline, asparagine and serine - for strain Fh-18 of <em>F. hepatica</em>. The results can be implemented in further optimization of the composition of the nutrient medium for culturing strains of Basidiomycetes wich producing carotenoids.</p> <p><em>Keywords: nitrogen-containing substances, Basidiomycetes, mycelium</em><em>,</em><em> culture filtrate, carotenoids</em></p>

Exemplar Abstract for Vibrio cholerae Pacini 1854 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Vibrio Cholerae

Exemplar Abstract for Vibrio cholerae Pacini 1854 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Vibrio Cholerae

Exemplar Abstract for Vibrio cholerae Pacini 1854 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Vibrio Cholerae
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