scholarly journals CLDN1 expression in cervical cancer cells is related to tumor invasion and metastasis

Oncotarget ◽  
2016 ◽  
Vol 7 (52) ◽  
pp. 87449-87461 ◽  
Author(s):  
Wei-na Zhang ◽  
Wei Li ◽  
Xiao-li Wang ◽  
Zheng Hu ◽  
Da Zhu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Tumor Invasion ◽  
Invasion And Metastasis ◽  
Cervical Cancer Cells

scholarly journals HAND2-AS1 inhibits invasion and metastasis of cervical cancer cells via microRNA-330-5p-mediated LDOC1

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Shengcai Chen ◽  
Jing Wang
Keyword(s):  
Cervical Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Cancer Cells ◽  
Cell Counting ◽  
Luciferase Reporter ◽  
Invasion And Metastasis ◽  
Node Metastasis ◽  
Cervical Cancer Cells ◽  
Associated Proteins

Abstract Background Cervical cancer is a serious disease with complicated pathogenesis and thus there is an urgent need to find novel targets for the treatment. Recently, long non-coding RNAs (lncRNAs) have emerged as critical factors in tumorigenesis. In this study, we aimed to investigate the mechanism of HAND2 antisense RNA 1 (HAND2-AS1) on the invasion and metastasis of cervical cancer cells. Methods The expression patterns of HAND2-AS1, microRNA-330-5p (miR-330-5p) and leucine zipper down-regulated in cancer 1 (LDOC1) in cervical cancer were characterized by RT-qPCR and western blot analysis. Dual luciferase reporter assay and RIP were applied to verify relationship between HAND2-AS1, miR-330-5p and LDOC1. Fluorescence in situ hybridization (FISH) was used to detect the subcellular localization of HAND2-AS1. Besides, viability, invasion and migration ability of HeLa cells were investigated by cell counting kit-8 (CCK-8) and Transwell assays respectively. Hematoxylin–eosin staining was performed for lymph node metastasis detection. In addition, the tumor growth in nude mice was evaluated. Results Low expression of HAND2-AS1 and LDOC1, and high expression of miR-330-5p were detected in cervical cancer tissues and cells. It was found that binding of HAND2-AS1 to miR-330-5p results in upregulation of LDOC1 expression. Also, overexpressed HAND2-AS1 and LDOC1 or down-regulated miR-330-5p inhibited expression of proliferation-associated proteins Ki-67, PCNA, migration-associated proteins N-cad and invasion-related proteins MMP-2, MMP-9 as well as lymph node metastasis. Moreover, HAND2-AS1 inhibited tumor formation and lymph node metastasis by binding to miR-330-5p in vivo. Conclusion HAND2-AS1 promotes LDOC1 expression by competitively binding to miR-330-5p and consequently inhibiting cervical cancer cell invasion and metastasis. This could facilitate development of therapeutic strategies against cervical cancer.

SIRT3 promotes the invasion and metastasis of cervical cancer cells by regulating fatty acid synthase

2019 ◽  
Vol 464 (1-2) ◽  
pp. 11-20 ◽  
Author(s):  
Li Xiu Xu ◽  
Li Jun Hao ◽  
Jun Qi Ma ◽  
Jing Kun Liu ◽  
Ayshamgul Hasim
Keyword(s):  
Cervical Cancer ◽  
Fatty Acid ◽  
Cancer Cells ◽  
Fatty Acid Synthase ◽  
Invasion And Metastasis ◽  
Cervical Cancer Cells

scholarly journals Targeted juglone blocks the invasion and metastasis of HPV-positive cervical cancer cells

2019 ◽  
Vol 140 (3) ◽  
pp. 211-217
Author(s):  
Xingyu Zhao ◽  
Wenhe Zhu ◽  
Ruowen Zhang ◽  
Mo Zhang ◽  
Jierui Zhao ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Invasion And Metastasis ◽  
Cervical Cancer Cells

scholarly journals Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 145
Author(s):  
Xiangchun Zhang ◽  
Qinqin Zheng ◽  
Ziqi Wang ◽  
Chao Xu ◽  
Haolei Han ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Quantitative Analysis ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Tumor Invasion ◽  
Inductively Coupled Plasma ◽  
Optical Microscope ◽  
Au Clusters ◽  
Icp Ms ◽  
Cervical Cancer Cells

Tumor invasion/metastasis is still the major cause of death in cancer patients. Membrane type-1 matrix metalloproteinase (MT1-MMP) is directly related to tumor invasion/metastasis. To accurately and quickly distinguish the risk of invasion/metastasis of primary tumor cells, it is urgent to develop a simple and precise quantitative method to distinguish the expression level of MT1-MMP. In this work, we have constructed red fluorescent Au clusters with peroxidase-like properties that could specifically bind to MT1-MMP on human cervical cancer cells. After MT1-MMP was labelled with Au clusters, we could visually see red fluorescence of MT1-MMP on cervical cancer cells via fluorescence microscopy and catalytic color imaging using an ordinary optical microscope. The constructed Au clusters contained 26 Au atoms; thus, the amount of MT1-MMP on cervical cancer cells could be accurately quantified using inductively coupled plasma mass spectrometry (ICP-MS). More importantly, the invasion/metastasis capabilities of the cervical cancer Siha, Caski and Hela cells with different MT1-MMP amounts could be accurately distinguished by fluorescence/catalysis qualitative imaging and ICP-MS quantitative analysis. This method of qualitative/quantitative analysis of tumor-associated proteins on cancer cells has great potential for accurately diagnosing aggressive tumor cells and assessment of their invasion/metastasis risk.

scholarly journals SLUG is Enhanced by Chemotherapeutics and Functions to Promote Invasion and Metastasis by Directly Targeting MMP3 in Cervical Cancer

2020 ◽  
Author(s):  
Senwei Jiang ◽  
Lan Zhang ◽  
Shuting Huang ◽  
Yun Zhou ◽  
Yanling Feng ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Lymph Node ◽  
Neoadjuvant Chemotherapy ◽  
Cancer Cells ◽  
Radical Surgery ◽  
Locally Advanced ◽  
Invasion And Metastasis ◽  
In Vivo Models ◽  
Cervical Cancer Cells ◽  
Cancer Tissues

Abstract BackgroundNeoadjuvant chemotherapy(NACT) is a widely used strategy in the treatment of locally advanced cancers. NACT followed by radical surgery for patients in FIGO stages IB2-IIB has proven to reduce tumor volume and numbers. Under certain conditions, however, NACT followed by radical surgery has not been found to improve overall survival, but rather to accelerate cancer growth and metastasis in current studies.MethodsIn this study, 35 paired pre/post-NACT cervical cancer tissues and 167 cervical cancer samples were examined by immunohistochemical analysis to investigate the role of SLUG in invasion and metastasis. Functional assays and a CHIP assay were performed to determine SLUG’s downstream genes and pathways. A lymph node metastatic mouse model was used to study the effects of SLUG- and MMP3-silencing on the malignant behavior of the cervical cancer cells. ResultsSLUG expression was potentially increased in cervical cancer tissues after neoadjuvant chemotherapy, which in turn positively correlated with pelvic lymph node metastasis. The expression of SLUG following neoadjuvant chemotherapy was identified as a poor survival indicator. SLUG overexpression promoted metastasis in cervical cancer in both in vitro and in vivo models, whereas SLUG silencing had the opposite effect. Mechanically, SLUG is not a prerequisite for EMT activation in cervical cancer models, but contributes to the invasiveness and metastasis of cervical cancer cells by directly tethering to the recognition sequence GCCAGGTGC in the MMP3 promoter region. ConclusionOur results provide mechanistic insight into the potential pro-cancer effect of neoadjuvant chemotherapy, and demonstrates that the SLUG-MMP3 axis could serve as a target for improving the efficacy of chemotherapy.

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