scholarly journals FAK regulates E-cadherin expression via p-SrcY416/p-ERK1/2/p-Stat3Y705 and PPARγ/miR-125b/Stat3 signaling pathway in B16F10 melanoma cells

Oncotarget ◽  
2017 ◽  
Vol 8 (8) ◽  
pp. 13898-13908 ◽  
Author(s):  
Guoshun Pei ◽  
Yan Lan ◽  
Dianhua Chen ◽  
Lina Ji ◽  
Zi-chun Hua
Keyword(s):  
Signaling Pathway ◽  
Melanoma Cells ◽  
B16f10 Melanoma ◽  
Stat3 Signaling ◽  
Stat3 Signaling Pathway ◽  
B16f10 Melanoma Cells ◽  
E Cadherin

New Synthesized Galloyl‐RGD Inhibits Melanogenesis by Regulating the CREB and ERK Signaling Pathway in B16F10 Melanoma Cells

2020 ◽  
Vol 96 (6) ◽  
pp. 1321-1331
Author(s):  
Seo Yeon Shin ◽  
Sang Ouk Sun ◽  
Jae Yeon Ko ◽  
Yun Seo Oh ◽  
Seung‐Sik Cho ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Melanoma Cells ◽  
Erk Signaling ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

scholarly journals Long Noncoding RNA HOST2 Promotes Epithelial-Mesenchymal Transition, Proliferation, Invasion and Migration of Hepatocellular Carcinoma Cells by Activating the JAK2-STAT3 Signaling Pathway

2018 ◽  
Vol 51 (1) ◽  
pp. 301-314 ◽  
Author(s):  
Yang  Wu ◽  
Tan Yuan ◽  
Wei-Wei Wang ◽  
Peng-Lei Ge ◽  
Zhi-Qiang Gao ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Noncoding Rna ◽  
Epithelial Mesenchymal Transition ◽  
Migration And Invasion ◽  
Mesenchymal Transition ◽  
Invasion And Migration ◽  
Stat3 Signaling ◽  
Stat3 Signaling Pathway ◽  
And Migration ◽  
E Cadherin

Background/Aims: This study aims to examine the effect of long noncoding RNA HOST2 (LncRNA HOST2) on epithelial-mesenchymal transition (EMT), proliferation, invasion and migration of hepatocellular carcinoma (HCC) cells via activation of the JAK2-STAT3 signaling pathway. Methods: HCC and para-cancerous tissues were collected from 136 HCC patients. Immunohistochemistry was used to detect the expression of JAK2 and STAT3. HCC SMMC7721 cells were grouped into blank, negative control (NC), HOST2 mimic and HOST2 inhibitor groups. The mRNA and protein expression levels of HOST2, JAK2, STAT3, E-cadherin, vimentin, Snail, Slug, Twist and Zeb1 in tissues and cells were determined by reverse transcription -quantitative polymerase chain reaction (RT-qPCR) and Western blotting, respectively. An MTT assay, scratch test and Transwell assay were applied to measure cell proliferation, migration and invasion, respectively. Results: The levels of JAK2, STAT3 and vimentin were higher in HCC tissues, while the expression of E-cadherin was lower in HCC tissues compared with para-cancerous tissues. The silencing of HOST2 significantly decreased cell proliferation, migration and invasion, reduced the levels of HOST2, JAK2, STAT3 and vimentin, and elevated the expression of E-cadherin. HOST2 silencing also decreased the levels of Snail, Slug and Twist but increased the level of Zeb1 protein, while the opposite findings were observed in the HOST2 mimic group. Conclusion: These results reveal a possible mechanism in HCC in which LncRNA HOST2 may increase EMT and enhance proliferation, invasion and metastasis of HCC cells via activation of the JAK2-STAT3 signaling pathway.

Annona squamosa L. leaves inhibit alpha‐melanocyte‐stimulating hormone (α‐MSH) stimulated melanogenesis via p38 signaling pathway in B16F10 melanoma cells

2019 ◽  
Vol 19 (7) ◽  
pp. 1785-1792
Author(s):  
Gyeong‐A Ko ◽  
Hye Rim Kang ◽  
Jeong Yong Moon ◽  
Meran Keshawa Ediriweera ◽  
Sangmi Eum ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Melanoma Cells ◽  
Annona Squamosa ◽  
B16f10 Melanoma ◽  
Melanocyte Stimulating Hormone ◽  
B16f10 Melanoma Cells ◽  
Stimulating Hormone

scholarly journals Ethanolic Extract of Hippocampus abdominalis Exerts Anti-Melanogenic Effects in B16F10 Melanoma Cells and Zebrafish Larvae by Activating the ERK Signaling Pathway

Cosmetics ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 1 ◽  
Author(s):  
Ilandarage Menu Neelaka Molagoda ◽  
Yung Hyun Choi ◽  
Seungheon Lee ◽  
Jiwon Sung ◽  
Cho Rong Lee ◽  
...  
Keyword(s):  
Heart Rate ◽  
Signaling Pathway ◽  
Ethanolic Extract ◽  
Adenosine Monophosphate ◽  
Melanoma Cells ◽  
Erk Signaling ◽  
B16f10 Melanoma ◽  
Zebrafish Larvae ◽  
B16f10 Melanoma Cells ◽  
Hippocampus Abdominalis

The big belly seahorse (Hippocampus abdominalis), a well-known ingredient of traditional medicine, possesses anti-inflammatory, anti-aging, anti-fatigue, and anti-thrombotic properties, and also increases male fertility. This study demonstrates that the ethanolic extract of dried H. abdominalis (EEHA) has anti-melanogenic effects in B16F10 melanoma cells and zebrafish larvae. EEHA significantly reduced the α-melanocyte-stimulating hormone (α-MSH)-induced melanogenesis in B16F10 melanoma cells without causing cytotoxicity. At a concentration of 200 µg/mL, EEHA had significant anti-melanogenic activity in zebrafish larvae, accompanied by a severe reduction in the heart rate (118 ± 17 heartbeats/min) compared to that of the untreated group (185 ± 8 heartbeats/min), indicating that EEHA induces cardiotoxicity at high concentrations. Below 100 µg/mL, EEHA significantly reduced melanogenesis in zebrafish larvae in the presence or absence of α-MSH, while the heart rate remained unaltered. Additionally, EEHA downregulated the release of cyclic adenosine monophosphate (cAMP) and the phosphorylation of cAMP response element-binding protein (CREB) in B16F10 melanoma cells, which inhibited microphthalmia-associated transcription factor (MITF), leading to the inhibition of tyrosinase activity. EEHA also increased the phosphorylation of extracellular-signal regulated kinase (ERK). The ERK inhibitor PD98059 interfered with the anti-melanogenic activity of EEHA in B16F10 melanoma cells and zebrafish larvae, indicating that the ERK signaling pathway might regulate the anti-melanogenic properties of EEHA. Altogether, we conclude that EEHA represses the cAMP–CREB–MITF axis, which consequently inhibits tyrosinase-mediated melanogenesis. We propose that at low concentrations, EEHA can serve as a promising anti-melanogenic agent that could be used to prepare whitening cosmetics and for treating melanogenic disorders.

Inhibitory Effects of Myelophycus simplex Papenfuss Methanol Extract on Melanogenesis in B16F10 Melanoma Cells

2017 ◽  
Vol 46 (1) ◽  
pp. 34-38
Author(s):  
Hyang Suk Kim ◽  
Ji Min Cheon ◽  
Da Hye Kwon ◽  
Eun Ok Choi ◽  
Min Ju Kim ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Melanoma Cells ◽  
Inhibitory Effects ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells
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