scholarly journals Transcriptomic profiles of human foreskin fibroblast cells in response to orf virus

Oncotarget ◽  
2017 ◽  
Vol 8 (35) ◽  
pp. 58668-58685 ◽  
Author(s):  
Daxiang Chen ◽  
Mingjian Long ◽  
Bin Xiao ◽  
Yufeng xiong ◽  
Huiqin Chen ◽  
...  



PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6267 ◽  
Author(s):  
Feng Pang ◽  
Mengmeng Zhang ◽  
Xiaojian Yang ◽  
Guohua Li ◽  
Shu Zhu ◽  
...  

Orf, caused by Orf virus (ORFV), is a globally distributed zoonotic disease responsible for serious economic losses in the agricultural sector. However, the mechanism underlying ORFV infection remains largely unknown. Circular RNAs (circRNAs), a novel type of endogenous non-coding RNAs, play important roles in various pathological processes but their involvement in ORFV infection and host response is unclear. In the current study, whole transcriptome sequencing and small RNA sequencing were performed in ORFV-infected goat skin fibroblast cells and uninfected cells. A total of 151 circRNAs, 341 messenger RNAs (mRNAs), and 56 microRNAs (miRNAs) were differently expressed following ORFV infection. Four circRNAs: circRNA1001, circRNA1684, circRNA3127 and circRNA7880 were validated by qRT-PCR and Sanger sequencing. Gene ontology (GO) analysis indicated that host genes of differently expressed circRNAs were significantly enriched in regulation of inflammatory response, epithelial structure maintenance, positive regulation of cell migration, positive regulation of ubiquitin-protein transferase activity, regulation of ion transmembrane transport, etc. The constructed circRNA-miRNA-mRNA network suggested that circRNAs may function as miRNA sponges indirectly regulating gene expression following ORFV infection. Our study presented the first comprehensive profiles of circRNAs in response to ORFV infection, thus providing new clues for the mechanisms of interactions between ORFV and the host.



PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e52756 ◽  
Author(s):  
Xian-Yang Qin ◽  
Hideko Sone ◽  
Yoshiyuki Kojima ◽  
Kentaro Mizuno ◽  
Katsuhiko Ueoka ◽  
...  


2006 ◽  
Vol 175 (4S) ◽  
pp. 165-166
Author(s):  
Benchun Liu ◽  
Guiting Lin ◽  
Emily J. Willingham ◽  
Hongxiu Ning ◽  
Ching-Shwun Lin ◽  
...  






PROTEOMICS ◽  
2015 ◽  
Vol 15 (17) ◽  
pp. 2953-2965 ◽  
Author(s):  
Juha Piltti ◽  
Markku Varjosalo ◽  
Chengjuan Qu ◽  
Jukka Häyrinen ◽  
Mikko J. Lammi


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