Ataxin-1 is involved in tumorigenesis of cervical cancer cells via the EGFR-RAS–MAPK signaling pathway

Hexokinase 2 (HK2) is a member of the hexokinases (HK) that has been reported to be a key regulator during glucose metabolism linked to malignant growth in many types of cancers. In this study, stimulation of HK2 expression was observed in squamous cervical cancer (SCC) tissues, and HK2 expression promoted the proliferation of cervical cancer cells in vitro and tumor formation in vivo by accelerating cell cycle progression, upregulating cyclin A1, and downregulating p27 expression. Moreover, transcriptome sequencing analysis revealed that MAPK3 (ERK1) was upregulated in HK2-overexpressing HeLa cells. Further experiments found that the protein levels of p-Raf, p-MEK1/2, ERK1/2, and p-ERK1/2 were increased in HK2 over-expressing SiHa and HeLa cells. When ERK1/2 and p-ERK1/2 expression was blocked by an inhibitor (FR180204), reduced cyclin A1 expression was observed in HK2 over-expressing cells, with induced p27 expression and inhibited cell growth. Therefore, our data demonstrated that HK2 promoted the proliferation of cervical cancer cells by upregulating cyclin A1 and down-regulating p27 expression through the Raf/MEK/ERK signaling pathway.

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MiR-1258 promotes the apoptosis of cervical cancer cells by regulating the E2F1/P53 signaling pathway

Experimental and Molecular Pathology ◽

10.1016/j.yexmp.2020.104368 ◽

2020 ◽

Vol 114 ◽

pp. 104368 ◽

Cited By ~ 5

Author(s):

Xing Peng ◽

Yumei Zhang ◽

Jinyu Gao ◽

Chunyan Cai

Keyword(s):

Cervical Cancer ◽

Cancer Cells ◽

Signaling Pathway ◽

P53 Signaling ◽

Cervical Cancer Cells ◽

P53 Signaling Pathway

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VERU-111 suppresses tumor growth and metastatic phenotypes of cervical cancer cells through the activation of p53 signaling pathway

Cancer Letters ◽

10.1016/j.canlet.2019.11.035 ◽

2020 ◽

Vol 470 ◽

pp. 64-74 ◽

Cited By ~ 1

Author(s):

Vivek K. Kashyap ◽

Nirnoy Dan ◽

Neeraj Chauhan ◽

Qinghui Wang ◽

Saini Setua ◽

...

Keyword(s):

Cervical Cancer ◽

Tumor Growth ◽

Cancer Cells ◽

Signaling Pathway ◽

P53 Signaling ◽

Cervical Cancer Cells ◽

P53 Signaling Pathway

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Overexpression of miR-142-5p suppresses the progression of cervical cancer through targeting PIK3AP1 expression

Molecular and Cellular Biology ◽

10.1128/mcb.00363-20 ◽

2020 ◽

Author(s):

Junliang Guo ◽

Tian Tang ◽

Jinhong Li ◽

Yihong Yang ◽

Yi Quan ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Cells ◽

Signaling Pathway ◽

Akt Signaling ◽

Migration And Invasion ◽

Akt Signaling Pathway ◽

Gain Function ◽

Cervical Cancer Cells ◽

Target Binding ◽

Cancer Tissues

The aim of current study was to explore the mechanism of miR-142-5p in cervical cancer through mediating the PIK3AP1/P13K/AKT axis. To this end, RT-qPCR and Western blot analysis results revealed that miR-142-5p was poorly expressed, whereas PIK3AP1 was highly expressed in cervical cancer tissues and cells. Furthermore, miR-142-5p was hypermethylated in cervical cancer, as reflected by MS-PCR and ChIP assessment of enrichment of DNMT1/DNMT3a/DNMT3b in the promoter region of miR-142-5p. A target binding relationship between miR-142-5p and PIK3AP1 was established, showing that miR-142-5p targeted and inhibited the expression of PIK3AP1. Loss- and gain- function assays were conducted to determine the roles of miR-142-5p and PIK3AP1 in cervical cancer cells. CCK-8, flow cytometry and Transwell assay results revealed that overexpression of miR-142-5p in cervical cancer cells downregulated PIK3AP1 and inhibited the P13K/AKT signaling pathway, leading to reduced proliferation, migration, and invasion capacity of cervical cancer cells, but enhanced apoptosis. Collectively, epigenetic regulation of miR-142-5p targeted PIK3AP1 to inactivate the P13K/AKT signaling pathway, thus suppressing development of cervical cancer, which presents new targets for the treatment of cervical cancer.

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