Pathogen Variability and New Sources of Resistance to Angular Leaf Spot Among Bean Landraces in Uganda

Angular leaf spot (ALS) caused by the fungus Pseudocercospora griseola is one of the most destructive disease in Latin America and eastern Africa countries. The fungus, P. griseola is highly variable and a diverse sources of resistance genes is required to manage this economically important disease. The use of genetic resistance is the most practical and economic way to manage angular leaf spot of the common bean. Common bean (Phaseolus vulgarise L.) germplasm were screened for resistance against Angular leaf spot (ALS) under field conditions at Wonodogenet and Areka Research farms. Out of 300 common bean accessions evaluated only 14 (4.6%) common bean accessions were resistant to naturally epidemics of angular leaf spot disease under field condition. Therefore, all common bean germplasm that showed resistance reaction can be involved in breeding program for the improvement of the common bean.

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Avances en la selección de fuentes de resistencia a las principales enfermedades del frijol común (Phaseolus vulgaris L.) en Costa Rica.

Agronomía Mesoamericana ◽

10.15517/am.v11i2.17302 ◽

2006 ◽

Vol 11 (2) ◽

pp. 25 ◽

Cited By ~ 2

Author(s):

Carlos M. Araya ◽

Rodolfo Araya

Keyword(s):

Leaf Spot ◽

Core Collection ◽

Breeding Program ◽

Colletotrichum Lindemuthianum ◽

Angular Leaf Spot ◽

Phaseolus Vulgaris L ◽

Sources Of Resistance ◽

Disease Reaction ◽

Growing Seasons ◽

Experimental Plots

In this research a broad set of bean genotypes were evaluated in the search for sources of resistance to both, anthracnose (Colletotrichum lindemuthianum) and angular leaf spot (Pahaeoisriopsis griseola). The germplasm evaluated came from the National Bean Breeding Program and international nurseries from CIAT: VIFURE, VIPADOGEN and CORE COLLECTION. Experimental plots were establised in Puriscal (1017 masl), Alajuela (814 masl) and Fraijanes (1650 masl), during the 97-98 and 98-99 growing seasons. Experimental plots were single or two rows from two to six m in length depending on seed availability. Disease reaction was scored at six and eight weeks after seeding using a 1 to 9 severity scale. Only genotypes showing 1-3 disease reaction for anthracnose and 1-4 for angular leaf spot were selected. In the germplas from the National Bean Breeding Program nine lines resistant to both pathogens were identified, of those, five lines were specifically resistant to anthracnose and two angular leaf spot. In the VIPADOGEN from CIAT, 25 lines were resistant to anthracnose and only two were resistant to both diseases. These materials also displayed adaptation to either drought or low soil fertility. The CORE COLLECTION nursery provided 82 lines resistant to anthracnose, 12 lines resistant to angular leaf spot and 26 lines were resistant to both pathogens. The most outstanding sources of resistance to anthracnose and angular leaf spot will be made available in a regional nursery.

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Reaction of common bean progenies to white mold derived from recurrent selection

Ciência Rural ◽

10.1590/s0103-84782014000400001 ◽

2014 ◽

Vol 44 (4) ◽

pp. 583-587 ◽

Cited By ~ 3

Author(s):

Danuza Araújo de Souza ◽

Fernanda Aparecida Castro Pereira ◽

Juliana Andrade Dias ◽

Monik Evelin Leite ◽

João Bosco dos Santos

Keyword(s):

Oxalic Acid ◽

Common Bean ◽

Leaf Spot ◽

Recurrent Selection ◽

Angular Leaf Spot ◽

White Mold ◽

High Yield ◽

Sources Of Resistance ◽

Original Population ◽

The One

This study was realized with the objective of verifying the resistance to white mold of common bean progenies derived from recurrent selection for resistance to angular leaf spot. The plant material used was obtained from a program of recurrent selection, which was started by crossing seven lines with carioca grain type with ten sources of resistance to angular leaf spot according to the partial diallel scheme. To evaluate the resistance to white mold, it was verified the reaction of 17 parents plus 35 selected progenies, to oxalic acid. Huge heterogeneity among the parents was observed, showing that some of them have resistance alleles to white mold, and thus, there is a possibility of recombine these alleles through the recurrent selection and obtaining progenies with high levels of resistance. Even in initial cycles, some progenies exhibited resistance to white mold similar to the one of cultivar 'G-122', which presents good level of resistance to this disease. This leads to infer that the original population already had some level of physiological resistance for the reaction to absorption of oxalic acid, and also for resistance to angular leaf spot. Thus, among the selected progenies for resistance to angular leaf spot, grain type and high yield, at least four progenies have resistance to white mold similar to the source of resistance 'G-122'.

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Fuentes de resistencia a la antracnosis y la mancha angular en frijol común para Costa Rica.

Agronomía Mesoamericana ◽

10.15517/am.v11i1.17328 ◽

2006 ◽

Vol 11 (1) ◽

pp. 11

Author(s):

Rodolfo Araya ◽

Floribeth Mora ◽

Shree P. Shingh

Keyword(s):

Costa Rica ◽

Leaf Spot ◽

Angular Leaf Spot ◽

Disease Score ◽

Sources Of Resistance ◽

Breeding Lines ◽

Resistant Disease ◽

La Mancha ◽

Abiotic And Biotic Factors ◽

Natural Incidence

The objective of this study was to select sources of resistance to angular leaf spot and anthracnose from a diverse group of germplasm with known resistance to these diseases and other abiotic and biotic factors. Atotal of 173 common beans that included 46 landraces and 127 breeding lines were evaluated at two locations (Estación Experimental Fabio Baudrit Moreno and Sub-Estación Experimental Fraijanes, both of Universidad de Costa Rica) in 1996 and 1997. A mixture of four races (races 9, 10, 547, and 1024 of maximum virulence and coverage in Costa Rica) of the anthracnose pathogen was used to inoculate four times. Because of high natural incidence of angular leaf spot, inoculation was not necessary. Twenty-one landraces and 13 breeding lines were resistant (disease score of 3 or less) to antracnose. Only five landraces (G 12529, G 14934, G 19428, G 19696, and G 19831), one improved cultivar (ICA Tundama), and a breeding line UCR 55 had intermediate reaction (disease score between 4 and 6). Thus, for angular leaf spot evaluation of a much broader range of germplasm of common bean and other related species is suggested.

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Interactions in the Brassica napus ‐ Pyrenopeziza brassicae pathosystem and sources of resistance to P . brassicae (light leaf spot)

Plant Pathology ◽

10.1111/ppa.13455 ◽

2021 ◽

Author(s):

C. S. Karandeni Dewage ◽

A. Qi ◽

H. U. Stotz ◽

Y. J. Huang ◽

B. D. L. Fitt

Keyword(s):

Brassica Napus ◽

Leaf Spot ◽

Sources Of Resistance

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Inheritance and development of molecular markers linked to angular leaf spot resistance genes in the common bean accession G10909

Molecular Breeding ◽

10.1007/s11032-010-9461-x ◽

2010 ◽

Vol 28 (1) ◽

pp. 57-71 ◽

Cited By ~ 17

Author(s):

George S. Mahuku ◽

María Antonia Henríquez ◽

Carmenza Montoya ◽

Carlos Jara ◽

Henry Teran ◽

...

Keyword(s):

Molecular Markers ◽

Common Bean ◽

Resistance Genes ◽

Leaf Spot ◽

Angular Leaf Spot ◽

The Common

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Response of Ugandan common bean varieties to Pseudocercospora griseola and Angular leaf spot disease development in varietal mixtures

International Journal of Pest Management ◽

10.1080/09670874.2016.1234656 ◽

2016 ◽

Vol 63 (2) ◽

pp. 119-127 ◽

Cited By ~ 3

Author(s):

N. Olango ◽

G. Tusiime ◽

J.W. Mulumba ◽

R. Nankya ◽

C. Fadda ◽

...

Keyword(s):

Common Bean ◽

Leaf Spot ◽

Angular Leaf Spot ◽

Leaf Spot Disease ◽

Disease Development ◽

Spot Disease ◽

Pseudocercospora Griseola

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Molecular Markers Dispute the Existence of the Afro-Andean Group of the Bean Angular Leaf Spot Pathogen, Phaeoisariopsis griseola

Phytopathology ◽

10.1094/phyto.2002.92.6.580 ◽

2002 ◽

Vol 92 (6) ◽

pp. 580-589 ◽

Cited By ~ 30

Author(s):

George S. Mahuku ◽

María Antonia Henríquez ◽

Jaime Munõz ◽

Robin A. Buruchara

Keyword(s):

Genetic Differentiation ◽

Leaf Spot ◽

Multiple Correspondence Analysis ◽

Gene Diversity ◽

Random Amplified Polymorphic Dna ◽

Point Mutations ◽

Angular Leaf Spot ◽

Gene Pools ◽

Phaeoisariopsis Griseola ◽

Intergenic Spacer Region

Coevolution of the angular leaf spot pathogen, Phaeoisariopsis griseola, with its common bean host has been demonstrated, and P. griseola isolates have been divided into Andean and Mesoamerican groups that correspond to defined bean gene pools. Recent characterization of P. griseola isolates from Africa has identified a group of isolates classified as Andean using random amplified polymorphic DNA (RAPD), but which are able to infect some Mesoamerican differential varieties. These isolates, designated Afro-Andean, have been identified only in Africa. Random amplified microsatellites, RAPD, and restriction digestion of amplified ribosomal intergenic spacer region were used to elucidate the relationships among the Afro-Andean, Andean, and Mesoamerican groups of P. griseola. Cluster and multiple correspondence analysis of molecular data separated isolates into Andean and Meso-american groups, and the Afro-Andean isolates clustered with Andean isolates. Analysis of molecular variance ascribed 2.8% of the total genetic variation to differences between Afro-Andean and Andean isolates from Africa. Gene diversity analysis revealed no genetic differentiation (GST = 0.004) between Afro-Andean and Andean isolates from Africa. However, significant levels of genetic differentiation (GST = 0.39) were observed between Afro-Andean or Andean isolates from Africa and Andean isolates from Latin America, revealing significant geographical differentiation within the Andean lineage. Results from this study showed that Afro-Andean isolates do not constitute a new P. griseola group and do not represent long-term evolution of the pathogen genome, but rather are likely the consequents of point mutations in genes for virulence. This finding has significant implications in the deployment of resistant bean genotypes.

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