scholarly journals SECADO A LA INTEMPERIE MEDIANTE APILADO EN CABALLETES Y CUADRADOS HORIZONTALES DE FERNANSÁNCHEZ (Triplaris cumingiana Fish. Mey.) Y LAUREL (Cordia alliodora Ruiz y Pav.) EN EL CANTÓN QUEVEDO, ECUADO

2009 ◽  
Vol 2 (1) ◽  
pp. 35-39
Author(s):  
Fidel Antonio Troya Mera ◽  
Guillermo Law Blanco ◽  
Pedro Suatunce Cunuhay
Keyword(s):  
Cordia Alliodora ◽  
Basal Media

Se evaluaron durante cinco meses, defectos de secado de la madera: abarquillados, combados, torceduras, rajaduras y grietas superficiales; así como también el contenido de equilibrio de humedad en las maderas de fernansánchez (T. cumingiana) y laurel (C. alliodora). Se compararon dos métodos de apilamiento: caballetes y cuadrados horizontales, en el secado de tablas de tres secciones del árbol (basal, media y terminal). No hubo diferencias significativas entre los métodos de apilamiento ni entre las secciones del árbol. Se determinó que es suficiente con 90 días de secado a la intemperie para que la madera de ambas especies alcance el contenido de humedad de equilibrio de la zona donde se realizó la investigación.

scholarly journals SECADO A LA INTEMPERIE MEDIANTE APILADO EN CABALLETES Y CUADRADOS HORIZONTALES DE FERNANSÁNCHEZ (Triplaris cumingiana Fish. Mey.) Y LAUREL (Cordia alliodora Ruiz y Pav.) EN EL CANTÓN QUEVEDO, ECUADO

2009 ◽  
Vol 2 (1) ◽  
pp. 35-39
Author(s):  
Fidel Antonio Troya Mera ◽  
Guillermo Law Blanco ◽  
Pedro Suatunce Cunuhay
Keyword(s):  
Cordia Alliodora ◽  
Basal Media

Se evaluaron durante cinco meses, defectos de secado de la madera: abarquillados, combados, torceduras, rajaduras y grietas superficiales; así como también el contenido de equilibrio de humedad en las maderas de fernansánchez (T. cumingiana) y laurel (C. alliodora). Se compararon dos métodos de apilamiento: caballetes y cuadrados horizontales, en el secado de tablas de tres secciones del árbol (basal, media y terminal). No hubo diferencias significativas entre los métodos de apilamiento ni entre las secciones del árbol. Se determinó que es suficiente con 90 días de secado a la intemperie para que la madera de ambas especies alcance el contenido de humedad de equilibrio de la zona donde se realizó la investigación.

scholarly journals SECADO A LA INTEMPERIE MEDIANTE APILADO EN CABALLETES Y CUADRADOS HORIZONTALES DE FERNANSÁNCHEZ (Triplaris cumingiana Fish. Mey.) Y LAUREL (Cordia alliodora Ruiz y Pav.) EN EL CANTÓN QUEVEDO, ECUADO

2009 ◽  
Vol 2 (1) ◽  
pp. 35
Author(s):  
Fidel Antonio Troya Mera ◽  
Guillermo Law Blanco ◽  
Pedro Suatunce Cunuhay
Keyword(s):  
Cordia Alliodora ◽  
Basal Media

Se evaluaron durante cinco meses, defectos de secado de la madera: abarquillados, combados, torceduras, rajaduras y grietas superficiales; así como también el contenido de equilibrio de humedad en las maderas de fernansánchez (T. cumingiana) y laurel (C. alliodora). Se compararon dos métodos de apilamiento: caballetes y cuadrados horizontales, en el secado de tablas de tres secciones del árbol (basal, media y terminal). No hubo diferencias significativas entre los métodos de apilamiento ni entre las secciones del árbol. Se determinó que es suficiente con 90 días de secado a la intemperie para que la madera de ambas especies alcance el contenido de humedad de equilibrio de la zona donde se realizó la investigación.

Modelling agroforestry systems of cacao (Theobroma cacao) with laurel (Cordia alliodora) or poro (Erythrina poeppigiana) in Costa Rica

1990 ◽  
Vol 12 (3) ◽  
pp. 229-249 ◽  
Author(s):  
J. Beer ◽  
A. Bonnemann ◽  
W. Chavez ◽  
H. W. Fassbender ◽  
A. C. Imbach ◽  
...  
Keyword(s):  
Costa Rica ◽  
Theobroma Cacao ◽  
Agroforestry Systems ◽  
Cordia Alliodora ◽  
Erythrina Poeppigiana

scholarly journals Human Adult Stem Cells Maintain a Constant Phenotype Profile Irrespective of Their Origin, Basal Media, and Long Term Cultures

2015 ◽  
Vol 2015 ◽  
pp. 1-29 ◽  
Author(s):  
Indumathi Somasundaram ◽  
Rashmi Mishra ◽  
Harikrishnan Radhakrishnan ◽  
Rajkumar Sankaran ◽  
Venkata Naga Srikanth Garikipati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adult Stem Cells ◽  
Expression Profiles ◽  
Subcutaneous Fat ◽  
Adult Stem Cell ◽  
Phenotypic Marker ◽  
Human Adult ◽  
Cell Based Therapy ◽  
Basal Media

The study aims to identify the phenotypic marker expressions of different human adult stem cells derived from, namely, bone marrow, subcutaneous fat, and omentum fat, cultured in different media, namely, DMEM-Low Glucose, Alpha-MEM, DMEM-F12 and DMEM-KO and under long term culture conditions (>P20). We characterized immunophenotype by using various hematopoietic, mesenchymal, endothelial markers, and cell adhesion molecules in the long term cultures (Passages-P1, P3, P5, P9, P12, P15, and P20.) Interestingly, data revealed similar marker expression profiles irrespective of source, basal media, and extensive culturing. This demonstrates that all adult stem cell sources mentioned in this study share similar phenotypic marker and all media seem appropriate for culturing these sources. However, a disparity was observed in the markers such as CD49d, CD54, CD117, CD29, and CD106, thereby warranting further research on these markers. Besides the aforesaid objective, it is understood from the study that immunophenotyping acts as a valuable tool to identify inherent property of each cell, thereby leading to a valuable cell based therapy.

scholarly journals Contemporaneous Production of Amylase and Protease through CCD Response Surface Methodology by Newly Isolated Bacillus megaterium Strain B69

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Rajshree Saxena ◽  
Rajni Singh
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Bacillus Megaterium ◽  
High Accuracy ◽  
Mass Scale ◽  
World Population ◽  
Oilseed Cake ◽  
Basal Media ◽  
Green Chemicals ◽  
Agroindustrial Wastes

The enormous increase in world population has resulted in generation of million tons of agricultural wastes. Biotechnological process for production of green chemicals, namely, enzymes, provides the best utilization of these otherwise unutilized wastes. The present study elaborates concomitant production of protease and amylase in solid state fermentation (SSF) by a newly isolated Bacillus megaterium B69, using agroindustrial wastes. Two-level statistical model employing Plackett-Burman and response surface methodology was designed for optimization of various physicochemical conditions affecting the production of two enzymes concomitantly. The studies revealed that the new strain concomitantly produced 1242 U/g of protease and 1666.6 U/g of amylase by best utilizing mustard oilseed cake as the substrate at 20% substrate concentration and 45% moisture content after 84 h of incubation. An increase of 2.95- and 2.04-fold from basal media was observed in protease and amylase production, respectively. ANOVA of both the design models showed high accuracy of the polynomial model with significant similarities between the predicted and the observed results. The model stood accurate at the bench level validation, suggesting that the design model could be used for multienzyme production at mass scale.

P–174 Globulin-rich protein supplements improve blastulation efficiency in culture and promote implantation in vitro

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
S Ojosnegros ◽  
A Seriola ◽  
E Aroca ◽  
A Godeau ◽  
D Denkova ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Culture Media ◽  
Blastocyst Stage ◽  
Reproductive Age ◽  
Human Embryos ◽  
Protein Supplement ◽  
Age Cohorts ◽  
The Matrix ◽  
Basal Media ◽  
Post Implantation

Abstract Study question Can globulin-rich compared to albumin (HSA) supplements improve blastulation and support embryo development towards post implantation? Summary answer Yes, globulin supplements with clinical-grade quality increase blastulation efficiency by 20% (50% in older mothers) and support the transition of embryos towards post-implantation development. What is known already During embryonic development at the morula stage there is a metabolic transition towards glycolysis as demand from outsourced energy increases. Therefore as cleavage proceeds, the demand for nutrients in the embryo increases accordingly. With few exceptions, HSA from human plasma or recombinant origin has been the main an only protein supplement used in almost all IVF-procedures. Globulin rich supplements are available but their use is not widespread and little is known about their efficiency in post-implantation development. Study design, size, duration We have cultured more than 600 mouse embryos in continuous media containing a protein supplement#1 (PS#1), from 1-cell up to blastocyst stage. At blastocyst stage embryos were replaced into fresh media containing protein supplement#2 (PS#2). The embryos were allowed to hatch naturally and then transferred into a proprietary matrix for further development and implantation for an additional 48h. Participants/materials, setting, methods: The blastulation rate, measured for HSA-supplemented embryo cohort was compared with embryos cultured in PS#1. Hatching efficiency was reported for embryos cultured in transfer media including PS#2. Once embedded in the matrix, advanced label-free imaging techniques and custom algorithms to measure matrix implantation strength were used. Key molecular markers (i.e. OCT4, CDX2) for correct post-implantation lineage patterning were documented by conventional 3D confocal immunofluorescence imaging. Main results and the role of chance Embryos supplemented with PS#1 reached blastocyst with overall 21% higher efficiency than embryos supplemented by HSA. When separated by age cohorts, embryos obtained from older females (ex-colony breeders, >14 weeks old) reached blastocyst stage with 55% higher efficiency than the same type of embryos cultured in the presence of HSA. Embryos obtained from females at optimal reproductive age reached blastocyst stage 10% more efficiently under PS#1 supplementation than with HSA. Hatching efficiency was 45% higher for embryos cultured with PS#2 than embryos supplemented with HSA. For every variable tested (e.g.% of arrested or degenerated embryos) or condition implemented (e.g. mouse basal media, human basal media from different brands, etc.) PS#1 and PS#2 outperformed, without exception, the supplementation with HSA. When embedded in the implantation matrix, the embryos cultured with PS#1 (cleavage) and transferred to PS#2 at blastocyst stage showed a remarkable implantation ability as measured by trophoblast outgrowth and matrix deformations. The embryos in PS#2 medium exerted stronger force into the matrix and also survived longer times than the embryos in HSA. PS#2 supported the transition of blastocyst towards post-implantation stages of development showing the correct lineage patterning of embryonic and extraembryonic molecular markers, including Oct4, CDx2, EOMES or GATA4. Limitations, reasons for caution This is a study based on an animal model. These observations need to be confirmed by ongoing experiments with human embryos. Wider implications of the findings: This work constitutes a proof-of-concept for the use of globulin-rich supplements as higher performance substitute of albumin in the culture of IVF embryos, both as (i) a standard protein source for culture media and (ii) as a supplement for transfer media to capacitate the embryo for implantation. Trial registration number Not applicable

CLASSIFICAÇÃO CLIMÁTICA PARA O SUL DO BRASIL UTILIZANDO O SISTEMA DE HOLDRIDGE (1967) / CLIMATIC CLASSIFICATION FOR SOUTHERN BRAZIL USING HOLDRIDGE (1967) SYSTEM

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Rafael Fausto de Lima ◽  
Lucas Eduardo de Oliveira Aparecido ◽  
João Antônio Lorençone ◽  
Pedro Antônio Lorençone ◽  
José Reinaldo da Silva Cabral de Moraes
Keyword(s):  
Energy Resources ◽  
Southern Brazil ◽  
Climatic Classification ◽  
Basal Media

O objetivo deste trabalho é classificar o clima da região Sul do Brasil utilizando o sistema de classificação climática por zonas de vidas estabelecido por Holdridge (1967). Foram utilizados um sequenciamento de 30 anos (1989-2019) de dados climáticos para região Sul do Brasil obtidos através da plataforma National Aeronautics and Space Administration/Prediction of Worldwide Energy Resources – (NASA/POWER). A evapotranspiração potencial (ETP) foi estimada utilizando o método definido por Camargo (1971). Através da combinação entre a precipitação anual, biotemperatura média anual, biotemperatura basal média anual, relação da evapotranspiração, delimitou-se as zonas de vidas para o sistema de Holdridge (1967). A temperatura e precipitação apresentaram médias anuais de 18,58 (± 1,57) °C e 1777 (± 239) mm respectivamente. Foram encontradas doze zonas de vidas para a região Sul, sendo as mais predominantes: Floresta subtropical de montana baixa úmida (31,37%), e Floresta subtropical premontana úmida (32,11%).

scholarly journals Efeito de curto prazo do tiotrópio nos portadores de DPOC em tratamento com β2-agonista

2010 ◽  
Vol 36 (2) ◽  
pp. 181-189 ◽  
Author(s):  
Frederico Leon Arrabal Fernandes ◽  
Vanessa Aparecida Leão Pavezi ◽  
Sérvulo Azevedo Dias Jr ◽  
Regina Maria Carvalho Pinto ◽  
Rafael Stelmach ◽  
...  
Keyword(s):  
Medical Research ◽  
Daily Living ◽  
Research Council ◽  
Medical Research Council ◽  
Activity Of Daily Living ◽  
Basal Media

OBJETIVO: Avaliar o impacto de curto prazo do uso de tiotrópio em pacientes com DPOC grave e muito grave com queixas de dispneia apesar do tratamento com outros broncodilatadores. MÉTODOS: Estudo prospectivo incluindo pacientes com DPOC grave ou muito grave, com queixa de dispneia de pequenos esforços ou ao repouso. A cada 15 dias, o tratamento broncodilatador foi modificado: salmeterol, tiotrópio e associação salmeterol+tiotrópio. Ao final de cada regime, foram realizados testes de função pulmonar e teste de caminhada de seis minutos (TC6). Também foram avaliados o grau de dispneia e a capacidade de realização de atividades de vida diária. Para a avaliação das atividades de vida diária, foi utilizada a escala London Chest Activity of Daily Living (LCADL) validado para uso no Brasil. RESULTADOS: Foram avaliados 52 pacientes. Desses, 30 completaram o estudo. A introdução de tiotrópio como monoterapia resultou em uma melhora significativa (p < 0,05) da dispneia basal (média do escore da escala do Medical Research Council de 3,0 para 2,5) e ao final do TC6 (média do escore da escala de Borg de 6,1 para 4,5), e as diferenças foram significativas (p < 0,05 para ambos). O uso da associação salmeterol+tiotrópio resultou em um aumento significativo médio de 81 mL no VEF1 e na melhora de 5,7 pontos no escore da escala LCADL. CONCLUSÕES: A introdução de tiotrópio no tratamento de pacientes com DPOC grave a muito grave em uso de β2-agonistas de longa duração causa melhora na função pulmonar e alivio sintomático perceptível pelos pacientes a curto prazo. Esses resultados, obtidos em regime de atendimento de vida real, dão suporte ao uso da associação salmeterol+tiotrópio em protocolos de assistência específicos a esses pacientes.

scholarly journals Chuva de sementes em remanescentes florestais de Campo Verde, MT

2014 ◽  
Vol 38 (4) ◽  
pp. 689-698
Author(s):  
William Pietro-Souza ◽  
Normandes Matos da Silva ◽  
Érica Pereira de Campos
Keyword(s):  
Cordia Alliodora ◽  
Mato Grosso

O objetivo deste trabalho consistiu em avaliar sazonalmente a produção de sementes provenientes de fragmentos florestais presentes na zona rural de Campo Verde, MT. Essa caracterização ocorreu em termos de produção de sementes e riqueza de espécies vegetais. Foram selecionados três fragmentos florestais caracterizados como Floresta Estacional Semidecidual. Em cada fragmento, cinco coletores foram instalados e confeccionados com canos de PVC (1 m²; malha de náilon de 0,001 m e 0,15 m de profundidade do bojo). O material foi coletado mensalmente, cujas sementes foram quantificadas e separadas conforme a síndrome de dispersão, etapa sucessional e forma de vida. Houve a determinação da densidade, frequência absoluta, índice de diversidade e equitabilidade. Ao todo, foram obtidas 3.622 sementes, pertencentes a 74 táxons. Maiores densidades de sementes foram observadas durante os meses de setembro, outubro e novembro. As lianas Gouania sp., Fridericia speciosa, Heteropterys sp. e Distictella sp. responderam com 55,95% do total de sementes aportadas. Das espécies que compuseram a chuva de sementes, 74% não são dispersas pelo vento. Todavia, quando se verificou a quantidade de diásporos coletados, formas dispersas pelo vento foram predominantes, representando 76% do total. Citam-se como táxons arbóreos importantes para a recomposição florestal na região de Campo Verde, situada no Sudeste de Mato Grosso: Tachigali rubiginosa, Siparuna guianensis, Nectandra sp., Cordia alliodora, Alibertia sp., Terminalia brasiliensis e Myrcia sp. Essas espécies devem ser alvo de estudos mais específicos relacionados ao seu potencial para recuperação de ambientes degradados, considerando-se as Áreas de Preservação Permanente e Reservas Legais.

scholarly journals Organogenesis of Dendrobium Orchid Using Traditional Media and Organic Extracts

1970 ◽  
pp. 30-35 ◽  
Author(s):  
S Akter ◽  
KM Nasiruddin ◽  
ABM Khaldun
Keyword(s):  
Key Words ◽  
Plantlet Regeneration ◽  
Coconut Water ◽  
Fresh Weight ◽  
Traditional Media ◽  
Organic Extracts ◽  
Half Strength ◽  
The Media ◽  
Basal Media ◽  
Weight Number

The study was undertaken with a view to investigate the effects of different basal media and organic extracts on organogenesis of Dendrobium orchid. Protocorm like bodies (PLBs) were cultured in Knudson C (KC), Vacin and Went (VW), half strength Murashige and Skoog (½MS) and New Phalaenopsis (NP) media supplemented with Sabri banana pulp (Sb), Charcoal (C) and Coconut water (Cw) organic extracts. KC, VW, ½MS and NP media significantly influenced the plantlet regeneration of Dendrobium orchid. Among the media, ½MS showed better performance on fresh weight, number PLBs, shoots and leaves per plantlet, The longest shoots were found in KC medium and maximum length of leaves was found in both KC and ½MS media. In case of different organic extracts Sb showed significantly better performance on fresh weights of PLBs, number of shoot and leaves per explant, length of shoots and leaves. Key words: Organogenesis, Dendrobium, media, organic extracts. DOI = 10.3329/jard.v5i1.1454 J Agric Rural Dev 5(1&2), 30-35, June 2007

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