Identification of Best Restorers and Maintainers in Rice Genotypes Suitable for Aerobic Cultivation with a CMS line (Oryza sativa L.)

2017 ◽  
Vol 5 (4) ◽  
pp. 1333-1336
Author(s):  
P. Madhukar ◽  
Rice ◽  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Ayumu Takatsuka ◽  
Tomohiko Kazama ◽  
Kinya Toriyama

Abstract Background Cytoplasmic male sterility (CMS) is a trait associated with non-functional pollen or anthers, caused by the interaction between mitochondrial and nuclear genes. Findings A Tadukan-type CMS line (TAA) and a restorer line (TAR) were obtained by successive backcrossing between the Oryza sativa cultivars Tadukan (a cytoplasmic donor) and Taichung 65 (a recurrent pollen parent). Using Illumina HiSeq, we determined whole-genome sequences of the mitochondria of TAA and screened the mitochondrial genome for the presence of open reading frame (orf) genes specific to this genome. One of these orf genes, orf312, showed differential expression patterns in TAA and TAR anthers at the meiotic and mature stages, with transcript amounts in TAR being less than those in TAA. The orf312 gene is similar to the previously described orf288, a part of which is among the components comprising WA352, a chimeric CMS-associated gene of wild-abortive-type CMS. Conclusions The orf312 gene is a promising candidate for CMS-associated gene in TAA.


2018 ◽  
Vol 6 (2) ◽  
pp. 120-129
Author(s):  
Abilio Tilman Baptista ◽  
Damanhuri ◽  
Nunun Barunawati

Rice is an important commodity for the majority of the population, especially in the continent of Asia use rice as a staple food. The state of Indonesia and East Timor, rice as the main food source that needs continue to increase because in addition to the population continues to grow at a rate of increase of about 2% per year, as well as the change in consumption pattern of the population of non-rice to the rice. Interest characterization and evaluation of the appearance of rice(Oryza Sativa L.) locally Timor Leste and Indonesian rice are getting Local rice genotypes Timorese suitable for cultivated in paddy fields. This research using a randomized block design (RBD) consisted of 9 treatments and 3 replications. The materials used are high-yielding rice varieties namely IR-3 64, Ciherang, Cimelati and 6 local rice genotypes Timor Leste, namely Hare Foam, Fafulu Hare, Hare Bauk Morin, Mean Hare, Hare and Hare Modok Fulan. Results showed that the highest plant length at the age of 10 MST contained in the local rice Hare Bauk Morin(134.67 cm), Number of tillers(40.30) the number of leaves(4.64).Flowering date and time of harvest shortest genotypes present in Morin namely Hare Bauk flowering date (39 days) and time of harvest (105 days). The highest number of panicles contained in Hare Mean genotype, high-yielding varieties IR64, Ciherang and Cimelati. The length of the longest panicle genotypes present in Foam Hare, Hare Hare Fafulu, and Fulan. The number of filled grains per panicle most contained in superior varieties IR64, Ciherang and Cimelati. The highest weight of 1000 grains contained in Cimelati varieties (vu3) with Hare Bauk Morin, IR64 and Cherang varieties. The highest rice yield for local rice genotypes present in Hare Bauk Morin (G3) of 9.79 ton.Ha-1 while yielding varieties there exist varieties Cherang (vu2) of 9.74 ton.Ha-1.


2018 ◽  
Vol 10 (25) ◽  
pp. 7-18
Author(s):  
Sanam Safaei Chaeikar ◽  
Babak Rabiei ◽  
Mehdi Rahimi ◽  
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2018 ◽  
Vol 16 (1) ◽  
pp. 44-54 ◽  
Author(s):  
M M Islam ◽  
E Kayesh ◽  
E Zaman ◽  
T A Urmi ◽  
M M Haque

Drought stress is a major constraint to the production and yield stability of crops. Rice (Oryza sativa L.) is considered as a drought-sensitive crop species. Within this species, there are considerable varietal differences in sensitivity to this environmental stress. An experiment was conducted at the laboratory of the Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Bangladesh during April to September 2016 to evaluate 100 rice genotypes for drought tolerance during germination and early seedling growth stage. The genotypes were tested against five levels of drought stress imposed by Polyethylene glycol 6000 (PEG-6000) @ 0, 5, 10, 15 and 20%. The experiment was laid out in a complete randomized design with four replications. The results showed that with increasing water stress, germination in all the genotypes decreased from 95.8% in control to 6.6 % in highest stress (20% PEG) level. Seedling height and dry weight also decreased in all rice genotypes with the increase in water stress level. Based on Standard Evaluation System (SES),18 genotypes were selected primarily. Among the 18 genotypes, Ratoil and Chinisakkar showed higher germination index, relative seedling height and relative seedling dry weight than the check drought tolerant BRRI dhan43 at 20% PEG. Beside these, performance of Kumridhan, Pusur and Somondori was also well at this level compared to BRRI dhan43. The genotypes Ratoil, Chinisakkar, Kumridhan, Pusur and Somondori showed the best performance under drought condition. The Agriculturists 2018; 16(1) 44-54


2017 ◽  
Vol 15 (1) ◽  
pp. 170-181
Author(s):  
MS Ahmed ◽  
Khandakar Md. Iftekharuddaula

Genetic improvement of rice (Oryza sativa L.) for yield is important for increasing demand of the growing population and the changing climate of the world. Recent studies showed that backcrossing twice using modern varieties as receptor and mini core collection as doner, most of the undesirable traits could be improved remarkably and in other words its maximum allele diversity could be brought back into rice fields. Core collection is defined as a subset chosen to represent the most genetic diversity of an initial collection with a minimum of redundancies. The objective of the present study was to review the selection of core collection of Jesso-Balam group of rice genotypes through quantitative, qualitative and molecular characters. Earlier, the same germplasms were characterized for agro-morphological, physico-chemical and molecular characters and grouped into different clusters by different methods at Bangladesh Rice Research Institute during 2009-12. Finally, the core collection was selected by reviewing the above characterized data and using the hierarchical cluster analysis. Moreover, the selection processes of core collection were improved by applying composite evaluation methods; such as agro-morphological traits, biochemical characters and so on, through sampling strategies based on genotypic values, predicted genotypic value, comparing different genetic distances, cluster methods and sampling strategies methods, molecular characterization or SSR marker base data. As a result, the selected core germplasm of Jesso-Balam rice accessions were JBPL1, JBPL8, JBPL9, JBPL10, JBPL13, JBPL15, JBPL16, JBPL17, JBPL19, JBPL20, JBPL21, JBPL23, JBPL25 and JBPL26. In conclusion, the core collection  need to be considered as the ‘working collection’ of Jesso-Balam rice genotypes for their easy and safe conservation and effective utilization in Gene bank.The Agriculturists 2017; 15(1) 170-181


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