DNA Barcoding and Biometric Investigation on the Invasive Oreochromis niloticus (Linnaeus, 1758) from the River Yamuna of Uttar Pradesh

2020 ◽  
Author(s):  
Absar Alam ◽  
Narinder Kumar Chadha ◽  
Annam-Pavan Kumar ◽  
Sushant Kumar Chakraborty ◽  
Kripal Datt Joshi ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
Dna Barcode ◽  
Taxonomic Status ◽  
River System ◽  
B Value ◽  
Coi Gene ◽  
Differential Growth ◽  
Male And Female ◽  
Total Length ◽  
River Yamuna

Oreochromis niloticus (Linnaeus, 1758), an invasive fish species is rapidly spreading in the Gangetic river system. The aim of the study was to identify and confirm its identity at species level by establishing a barcoding reference datasets in the river Yamuna and then to investigate its morphometric, meristic traits and length-weight relationship of O. niloticus from the Allahabad water of the river Yamuna by the examination of 341 fish specimens collected during October 2011 to September 2012. The taxonomic status of O. niloticus (Linnaeus, 1758), in the river Yamuna was assessed using DNA barcode marker COI gene sequences, have clearly identified the O. niloticus species with 100% similarity value with public database. The length-weight relationships were established as W = 0.029486L2.881638 and W = 0.058499L2.661735 for male and female. Differential growth in length-weight between male and female of O. niloticus were significant (p less than 0.05), with a greater slope (b) value for male (2.88) than female (2.66). The t statistics estimated for the regression coefficients (b) were significant at 5% level of significance (p less than 0.05), indicating an allometric growth pattern. The correlation was highest for the standard length and the total length (0.9878) and minimum for caudal peduncle length and total length (0.8022). Fin formula based on the meristic studies can be written as B3, D 15-19/11-15, P14, V 1/5, A 3/8-11 and C16-22.

New species of Endoclita (Lepidoptera: Hepialidae) and revived species status of E. kosemponis from Taiwan

Zootaxa ◽  
2019 ◽  
Vol 4551 (4) ◽  
pp. 432
Author(s):  
ULF BUCHSBAUM ◽  
JOHN R. GREHAN
Keyword(s):  
New Species ◽  
Taxonomic Status ◽  
Female Genitalia ◽  
Coi Gene ◽  
Species Status ◽  
External Appearance ◽  
Male And Female ◽  
Preliminary Comparison ◽  
Future Evaluation ◽  
First Time

Two species of Endoclita C. &. R. Felder, 1874 from Taiwan are evaluated for their taxonomic status: Endoclita meifenga Buchsbaum & Grehan sp. n. is described as new for a unique male from the central mountains of Taiwan, and E. kosemponis (Strand, 1916), stat. rev. is elevated to species status. We show that the forewing markings and genitalic characteristics of E. meifenga sp. n. distinguish this species from all other Endoclita. The external appearance of E. kosemponis is similar to E. sinensis (Moore, 1877) but the two species show distinct differences in the male and female genitalia. Preliminary comparison of E. sinensis from Taiwan and Japan suggests they represent more than one species. A sample of the COI gene was sequenced for E. meifenga sp. n. and the primary types of E. meifenga sp. n. and E. kosemponis sp. n. are illustrated here for the first time. The taxonomic status of the non endemic Taiwanese E. davidi (Poujade, 1886) and E. sinensis needs future evaluation. 

scholarly journals Taxonomic decision as a compromise: Acasis appensata (Eversmann, 1832) in Central Italy—a case of conflicting evidence between DNA barcode and morphology (Lepidoptera: Geometridae)

Zootaxa ◽  
2011 ◽  
Vol 3070 (1) ◽  
pp. 60 ◽  
Author(s):  
AXEL HAUSMANN ◽  
PETER HUEMER
Keyword(s):  
Central Italy ◽  
Dna Barcode ◽  
Taxonomic Status ◽  
Female Genitalia ◽  
External Appearance ◽  
Male And Female ◽  
Conflicting Evidence ◽  
Taxonomic Decision ◽  
First Time ◽  
Range Of Variation

Acasis appensata (Eversmann, 1842) (Lepidoptera: Geometridae), distributed from central and northern Europe to eastern Asia, was collected in the Mediterranean (Central Italy, Abruzzi) for the first time. Adults from this region differ significantly from nominotypical populations in external appearance and in the 5’ barcode fragment of the CO1 gene (barcode). However, morphology of male and female genitalia falls completely within the range of variation of A. appensata, resulting in conflicting evidence with respect to its taxonomic status. Considering the striking differences in genitalia morphology between the two European species of Acasis, viz. A. appensata and A. viretata (Hübner, 1799), the population from the Abruzzi is not validated as different at species level but described as the subspecies A. appensata callaina ssp. nov. The habitus and male and female genitalia of European taxa are figured.

scholarly journals KARAKTER MORFOMETRIK DAN MERISTIK IKAN EKOR PEDANG (Xiphophorus helleri Heckel, 1848) DI DANAU BUYAN, BULELENG, BALI

2019 ◽  
Vol 11 (2) ◽  
pp. 103
Author(s):  
I Nyoman Yoga Parawangsa ◽  
Prawira A. R. P Tampubolon ◽  
Nyoman Dati Pertami
Keyword(s):  
B Value ◽  
The Body ◽  
Freshwater Ecosystem ◽  
Meristic Characters ◽  
Morphometric Characters ◽  
Male And Female ◽  
Total Length ◽  
Length Relationship ◽  
Different Color ◽  
D 12

Ikan ekor pedang bukan merupakan ikan asli Indonesia. Ikan ini berasal dari Amerika Tengah dan dilaporkan menyebabkan kerugian di beberapa perairan yang dihuninya. Ikan ekor pedang merupakan ikan kedua yang paling banyak tertangkap di Danau Buyan. Penelitian ini bertujuan untuk mengungkap karakter morfometrik dan meristik, hubungan panjang bobot dan hubungan panjang total-panjang baku pada dua varian ikan ekor pedang di Danau Buyan yang memiliki warna tubuh yang berbeda. Penelitian dilakukan pada bulan Januari sampai April 2018. Ikan yang diamati berjumlah 160 ekor. Pengamatan meliputi pengukuran 12 karakter morfometrik tradisional, 14 karakter truss morphometric, penghitungan empat karakter meristik, dan penimbangan bobot. Panjang total ikan ekor pedang jantan dan betina pada varian I adalah 43,94 - 79,47 mm dan 43,81 - 115,80 mm dengan nilai b= 2,90 dan b= 2,98. Kemudian, panjang total ikan ekor pedang pada varian II adalah 45,76 - 83,91 mm untuk ikan jantan dan 41,43 - 88,49 mm untuk ikan betina dengan nilai b= 2,80 dan b= 3,07. Karakter meristik pada kedua varian ikan ekor pedang baik jantan dan betina adalah D. 12-13 ; A. 8 - 9. Hubungan panjang bobot ikan ekor pedang pada kedua varian menunjukan pertumbuhan isometrik. Berdasarkan pengamatan dalam penelitian ini, diketahui tidak terdapat perbedaan pada dua varian ikan ekor pedang di Danau Buyan.Green swordtail is not originally from Indonesia, but from Central America. This fish was reported harmful in some freshwater ecosystem. Green swordtail was the second most caught fish in Buyan Lake. The aims of this research were to reveal morphometric and meristic characters, length-weight relationship and total length-standard length relationship on two variants of green swordtail with the different color in Buyan Lake. This research was conducted from January to April 2018 in Buyan Lake. The number of measured and weighed fish were 160 individuals. There were 12 traditional morphometric characters, 14 truss morphometric characters, and four meristic characters observed. Respectively, the total length for green swordtail variant I male and female were 43.94 - 79.47 mm and 43.81 - 115.80 mm. The b value for both of the variant I were 2.90 and 2.98. For the variant II, the total length was 45.76 - 83.91 mm for male fish and 41.43 - 88.49 for female. The b value for variant II were 2.80 and 3.07 for male and female respectively. Meristic characters for all variant and sex was D 12 - 13; A 8 - 9. The growth pattern was isometric. There is no difference in the body shape between the variant of green swordtail fish.

DNA barcoding unravels three new species and a subspecies of Olepa Watson, 1980 (Lepidoptera, Erebidae, Arctiinae) from India, with morphotypes

2020 ◽  
Vol 19 (2) ◽  
pp. 44-60
Author(s):  
APARNA SURESHCHANDRA KALAWATE ◽  
K. P. DINESH ◽  
A. SHABNAM
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Dna Sequences ◽  
Dna Barcode ◽  
Coi Gene ◽  
Male And Female ◽  
Morphological Variations ◽  
Three New Species ◽  
First Time ◽  
Respective Species

The genus Olepa is distributed in Palearctic and Oriental regions with more species in India and Sri Lanka. In the recent studies, morphological variations within the group were well established, with couple of first set of mt COI DNA barcodes for at least three species. In the present account, three new species and a new subspecies are described from the northern Western Ghats region of Maharashtra based on mt COI DNA barcode studies. Due to high morphological divergence and complete genetic homogeneity on the mt COI DNA, four morphotypes under two species are reported. Morphological and genital characters of male and female are provided along with their respective species morphotypes for the first time under this genus from India. The genitalia and the habitus of male and female are illustrated. Preliminary phylogenetic tree based on the mt COI DNA sequences available in the GenBank for the genus with the sequences for the new species also provided and discussed. Key words: new taxa, morphotype, Maharashtra, DNA barcoding, mt COI gene

Studies on the growth and mortality of invasive Oreochromis niloticus (Linnaeus, 1758) in sub-tropical river Yamuna, part of Gangetic River system, India

2019 ◽  
Vol 22 (4) ◽  
pp. 473-480
Author(s):  
Absar Alam ◽  
Narendra Kumar Chadha ◽  
Sushant Kumar Chakraborty ◽  
Kripal Datt Joshi ◽  
Tarkeshwar Kumar ◽  
...  
Keyword(s):  
Oreochromis Niloticus ◽  
River System ◽  
Tropical River ◽  
River Yamuna

scholarly journals Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode

2018 ◽  
Vol 19 (6) ◽  
pp. 1985-1992
Author(s):  
DWI SENDI PRIYONO ◽  
DEDY D. SOLIHIN ◽  
ACHMAD FARAJALLAH ◽  
DIAH IRAWATI DWI ARINI
Keyword(s):  
Endemic Species ◽  
Conservation Management ◽  
Dna Barcode ◽  
Taxonomic Status ◽  
Species Level ◽  
Coi Gene ◽  
Cytochrome Oxidase Subunit ◽  
Taxonomic Problem ◽  
Cytochrome Oxidase Subunit 1 ◽  
Taxonomic Studies

Priyono DS, Solihin DD, Farajallah A, Arini DID. 2018. Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode. Biodiversitas 19: 1985-1992. Anoa is an endangered endemic species in Sulawesi. The controversial issue of anoa conservation until now is the taxonomic status of lowland and mountain anoa. This study aims to test the ability of DNA barcoding techniques to identify the taxonomy between mountain anoa and lowland anoa. A 681bp fragment of cytochrome oxidase subunit 1 (COI) gene was obtained and used to solve the molecular taxonomic problem and to resolve the phylogenetic relationships of the two types of anoa. Our results showed that the DNA barcode is useful in assigning the taxonomic position of anoa. In the phylogenetic tree, we found that the two types of anoa were in separate clades. We also found that based on the Kimura-2 parameter (K2P), the genetic distance between the two types of anoa showed higher values (3.4%) than the threshold of the separating species level. We, therefore, proposed that the binomial nomenclature for both types of lowland and mountain anoa are respectively Bubalus depressicornis and Bubalus quarlesi. We suggest that the use of DNA barcode techniques in anoa taxonomic studies and their implementation will be useful in conservation management

Notes on the molecular taxonomy of the Proclossiana eunomia complex (Lepidoptera, Nymphalidae: Argynnini): analysis of DNA barcodes

2018 ◽  
Vol 8 (1) ◽  
pp. 222-232 ◽  
Author(s):  
R. V. Yakovlev ◽  
N. A. Shapoval ◽  
G. N. Kuftina ◽  
A. V. Kulak ◽  
S. V. Kovalev
Keyword(s):  
Body Size ◽  
Molecular Taxonomy ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Coi Gene ◽  
Wing Pattern ◽  
Molecular Approach ◽  
Dna Barcodes ◽  
Morphological Differences ◽  
Proclossiana Eunomia

The Proclossiana eunomia (Esper, 1799) complex is currently composed of the several subspecies distributed throughout Palaearсtic region and North America. Despite the fact that some of the taxa have differences in wing pattern and body size, previous assumptions on taxonomy not supported by molecular data. Therefore, the identity of certain populations of this complex has remained unclear and the taxonomic status of several recently described taxa is debated. Here, we provide insights into systematics of some Palaearctic members of this group using molecular approach, based on the analysis of the barcoding fragment of the COI gene taking into account known morphological differences.

Alaskan moose measurements and weights and measurement–weight relationships

1978 ◽  
Vol 56 (2) ◽  
pp. 298-306 ◽  
Author(s):  
Albert W. Franzmann ◽  
Robert E. LeResche ◽  
Robert A. Rausch ◽  
John L. Oldemeyer
Keyword(s):  
Growth Rate ◽  
Blood Parameters ◽  
Field Application ◽  
Regression Equations ◽  
Body Measurements ◽  
Male And Female ◽  
Total Length ◽  
Chest Girth ◽  
Hind Foot ◽  
Alces Alces Gigas

A total of 1329 Alaskan moose (Alces alces gigas) were measured for total length, 1340 for chest girth, 1317 for hind foot, 618 for shoulder height, and 1219 for ear length. Five hundred and four moose were weighed. These data were classified by sex, age, month sampled, and location. Growth rates were plotted and discussed. No morphometric differences were detected by sex and location classifications up to age 36 months. Location differences were detected which corresponded to general condition, productivity, and condition related blood parameters of the populations. Seasonal weight patterns demonstrated increases from 21 to 55% from spring to late fall. Measurements, particularly total lengths, were better indicators of growth rate than were weights. All body measurements were significantly correlated with weight: total length, r (correlation coefficient) = 0.94; chest girth, r = 0.90; shoulder height, r = 0.87; and hind foot, r = 0.81. Combined sex regression equations were derived because the slight differences between the male and female regression lines were unimportant in field application. These regression equations were as follows: weight (kilograms) = −239.7 + 2.07(total length); weight (kilograms) = −245.3 + 3.14(chest girth).

DNA barcodes provide evidence for the independent origin of the cultivated silkworms Samia ricini and Samia cynthia

2021 ◽  
pp. 1-8
Author(s):  
J.-C. Huang ◽  
X.-Y. Li ◽  
Y.-P. Li ◽  
R.-S. Zhang ◽  
D.-B. Chen ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Phylogenetic Relationship ◽  
Dna Barcode ◽  
Distinct Species ◽  
Coi Gene ◽  
Molecular Evidence ◽  
Dna Barcodes ◽  
Close Relationship ◽  
Molecular Phylogenetic ◽  
Phylogenetic Framework

Samia ricini (Wm. Jones) and Samia cynthia (Drury) (Lepidoptera: Saturniidae) have been used as traditional sources of food as well as silk-producing insects. However, the phylogenetic relationship between the two silkworms remains to be addressed. In this study, the mitochondrial cytochrome c oxidase subunit 1 (COI) gene sequences corresponding to DNA barcodes from 13 Samia species were analysed, and a DNA barcode-based phylogenetic framework for these Samia species was provided. Phylogenetic analysis showed that multiple individuals of a species could be clustered together. Our analysis revealed a close relationship among Samia yayukae Paukstadt, Peigler and Paukstadt, Samia abrerai Naumann and Peigler, Samia kohlli Naumann and Peigler, Samia naessigi Naumann and Peigler, Samia naumanni Paukstadt, Peigler and Paukstadt, and Samia kalimantanensis Paukstadt and Paukstadt. The mixed clustering relationship and low Kimura-2-parameter (K2P) genetic distance (0.006) between individuals of S. ricini and Samia canningi (Hutton) indicated that the cultivated silkworm S. ricini was derived from the non-cultivated silkworm S. canningi. The remote phylogenetic relationship and high K2P genetic distance (0.039) indicated that S. ricini and S. cynthia are distinct species, thus providing solid molecular evidence that they had entirely independent origins. The relationships between S. kalimantanensis and S. naumanni and between S. cynthia and Samia wangi Naumann and Peigler, as well as the potential cryptic species within S. abrerai were also discussed. This is the first study to assess the DNA barcodes of the genus Samia, which supplements the knowledge of species identification and provides the first molecular phylogenetic framework for Samia species.

A new species of the genus Parapentacentrus Shiraki, 1930 from Yunnan, China (Orthoptera: Gryllidae: Itarinae)

Zootaxa ◽  
2021 ◽  
Vol 5032 (1) ◽  
pp. 143-146
Author(s):  
YI-JIAO LIU ◽  
ZHU-QING HE
Keyword(s):  
New Species ◽  
Male Genitalia ◽  
Dna Barcode ◽  
Coi Gene ◽  
East China ◽  
Anal Plate ◽  
Type Specimens ◽  
Normal University ◽  
One New Species ◽  
A New Species

The genus Parapentacentrus Shiraki, 1930 includes two species with long wings. In this paper, we report one new species, P. brevipennis He sp. nov., from Jinping, Yunnan, China. The new species have short forewings and hindwings, and have differences in the shape of supra-anal plate and male genitalia. DNA Barcode (COI gene) of this new species are provided. The type specimens are deposited in Museum of Biology, East China Normal University (ECNU).  

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