Characteristics and freezability of Assam Hill goat semen

2017 ◽  
Author(s):  
S. Deori ◽  
B. C. Deka ◽  
R. K. Biswas ◽  
N. Nahardeka ◽  
A. Arangasamy ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Mean Values ◽  
Acceptable Quality ◽  
Sperm Abnormality ◽  
Semen Characteristics ◽  
The Mean ◽  
Live Sperm

Assam Hill goat (AHG) is an important goat germplasm found in Assam and its adjoining areas of India. The study was designed with an objective to study the semen characteristics and freezability of AHG buck semen using Tris -Egg yolk-Citrate-Fructose diluent. The mean values of fresh semen characteristics in AHG bucks viz., ejaculate volume (ml), initial sperm motility (%), sperm concentration (x106/ml), live sperm (%), sperm abnormality (%), HOST-reacted sperm (%) and intact acrosome (%) recorded were 0.39 ± 0.01, 77.97 ± 0.73, 3201.00 ± 143.78, 83.02 ± 0.65, 7.66 ± 0.73, 66.95 ± 0.74 and 93.34 ± 0.51, respectively. Mean values for post-thaw semen characteristics i.e., sperm motility (%), live sperm (%), HOST-reacted sperm (%) and intact acrosome (%) were 55.39 ± 0.97, 71.01 ± 0.78, 54.77 ± 0.55 and 82.16 ± 0.43, respectively. It can be concluded that AHG bucks donate acceptable quality of semen which can be frozen successfully in Tris-Egg yolk-Citrate-Fructose diluents for using in Artificial Insemination.

scholarly journals The effect of freezing on cryosurvival of yak sperm

2018 ◽  
Vol 15 (2) ◽  
pp. 215-218
Author(s):  
S Deori
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Mean Values ◽  
Frozen Semen ◽  
Sperm Abnormality ◽  
Semen Characteristics ◽  
Live Sperm

A study was carried out to study the effect of freezing on cryosurvival of yak semen. Artificial insemination in yak is still in infancy. Semen cryopreservation and use of artificial insemination can be applied in yak husbandry for conservation and rapid multiplication of superior germplasm. Semen was collected from four adult yak bulls using artificial vagina method managed under uniform conditions. A total of 40 ejaculates comprising of 10 ejaculates each bull were collected following twice a week schedule and evaluated for fresh semen characteristics. The fresh yak semen characteristics viz. ejaculate volume (ml), mass activity (0-4), initial sperm motility (%), sperm concentration (x 106/ml), live sperm (%), sperm abnormality (%) and intact acrosome (%) were 3.10 ± 0.18, 3.53 ± 0.96, 83.89 ± 2.87, 1180.22 ± 42.32, 77.63 ± 4.23, 8.45 ± 3.33 and 93.61 ± 3.78 respectively. The ejaculates were diluted (1:10) with Tris extender consisting of 6.4 ml glycerol and 20 ml of fresh egg yolk. Straws were equilibrated at 5°C for 4 hours followed by exposure to liquid nitrogen vapour for 10 minutes and finally transferred to liquid nitrogen container for storage. The cryosurvival rate was studied after 7 days of storage in liquid nitrogen. The frozen semen was thawed in warm water (37°C) for 30 seconds for evaluation. Mean values of postthaw sperm motility (%), live sperm (%) and intact acrosome (%) in yaks were 55.67 ± 4.67, 65.62 ± 3.23 and 89.26 ± 3.67 respectively. In conclusion, yak semen has a better cryosurvival while freezing in tris extender with 6.4 per cent glycerol and 20 per cent egg yolk following an equilibration period of 4h.SAARC J. Agri., 15(2): 215-218 (2017)

scholarly journals Raw Semen Characteristics of Three Different Indonesian Local Roosters

2016 ◽  
Vol 18 (3) ◽  
pp. 165
Author(s):  
Emilia Kamung Hambu ◽  
Raden Iis Arifiantini ◽  
Bambang Purwantara ◽  
Sri Darwati
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Mass Movement ◽  
Sperm Concentration ◽  
Semen Volume ◽  
Sperm Abnormality ◽  
Microscopic Evaluation ◽  
Semen Characteristics ◽  
Live Sperm ◽  
Better Than

Indonesia has agreat variety of roosters, either indigenous type as well as exotic and cross breed. The purpose of this experiment was to study the characteristics of semen from three types of Indonesian local roosters such as Merawang, Kampung and crosses Sentul Kampung with Kedu (SK Kedu). A total of 15 roosters consist of  Merawang roosters,  Kampung, and SK Kedu roosters were 5 each. The semen was collected 3 times a week by dorso-abdominal and cloaca massage method. The parameters evaluation was macroscopic characteristics consist of volume, color, consistency, and pH. Microscopic evaluation of semen such as a mass movement, sperm motility, live sperm, sperm abnormality and sperm concentration. Results of this experiment showed that semen volume of  Merawang  (0.40±0.26 mL) was higher (p<0.05) compare to Kampung (0.24±0.12 mL) or  SK Kedu (0.16±0.10 mL) but no difference on semen color, consistency and semen pH. There were no difference in the mass movement, sperm motility and live sperm as well as on sperm abnormality among three types of roosters.  Sperm  concentration of Merawang (4490 million mL-1) was significantly higher than Kampung (3245 million mL-1) and the SK Kedu roosters (3751 million mL-1). Its was conclude that Merawang roosters had good semen quality better than Kampung and SK Kedu roosters

scholarly journals Semen characteristics of the endangered cyprinid lake minnow, Eupallasella percnurus (Pall.), from different Polish populations

2020 ◽  
Vol 28 (4) ◽  
pp. 200-204
Author(s):  
Justyna Sikorska ◽  
Sylwia Judycka ◽  
Rafał Kamiński ◽  
Jacek Wolnicki
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Artificial Reproduction ◽  
Sperm Velocity ◽  
Mean Values ◽  
Semen Characteristics

Abstract The aim of the study was to compare the quality and quantity parameters of semen in male lake minnow, Eupallasella percnurus (Pallas, 1814), from six Polish populations. Substantial differences among the populations were found in milt volume (36–112 µl) and sperm concentration (4.7–8.0 × 109 ml−1). Mean values for sperm motility were similar; however, there were population-related differences in sperm velocity measured with VSL (96.3–127 µm s−1). Despite this, the quality of semen in all cases was high and appropriate for both artificial reproduction and cryopreservation.

Fresh and Post-Thaw Seminal Attributes of Jafarabadi Buffalo Bulls

2020 ◽  
Vol 15 (03) ◽  
pp. 45-48
Author(s):  
K. H. Parmar ◽  
FS Kavani ◽  
TK Patbandha ◽  
SS Parikh
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Conception Rate ◽  
Mean Values ◽  
Abnormal Sperm ◽  
The Mean ◽  
Live Sperm ◽  
One Year ◽  
Acrosomal Integrity

The study was conducted on four Jafarabadi breeding bulls, 5-6 years old to know the fresh and post-thawed seminal characteristics based on total of 192 semen ejaculates evaluated and cryopreserved over one year period. The mean values of fresh neat seminal characteristics of Jafarabadi bulls, viz., ejaculate volume (ml), colour/density (score), sperm concentration (million/ml), mass activity (score), initial motility (%), live sperm (%), abnormal sperm (%), HOS reactive sperm (%) and acrosomal integrity (%) were 5.19±0.18, 2.38±0.10, 1253.36±24.75, 3.73±0.05, 80.31±0.05, 86.20±0.64, 5.00±0.40, 85.75±0.43 and 93.56±0.56, respectively, whereas the mean post-thawed sperm characteristics, viz., progressive sperm motility, live sperm (%), abnormal sperm (%), HOS reactive sperm (%), acrosomal integrity (%) and first insemination conception rate (%) observed were 57.60±0.36, 66.34±0.53, 8.85±0.33, 56.97±0.46, 75.26±0.17 and 44.63±0.14, respectively. The semen quality of fresh and post-thawed samples observed was within normal limit for use in breeding program with satisfactory first insemination conception rate.

scholarly journals EPIDIDYMAL SPERMATOZOA QUALITY OF ETAWA CROSSBREED GOAT IN TRIS EXTENDER SUPPLEMENTED WITH VARIOUS LACTOSE CONCENTRATIONS

2017 ◽  
Vol 11 (1) ◽  
pp. 15-18
Author(s):  
Muhammad Riyadhi ◽  
Agus Setiawan ◽  
Herdis Herdis ◽  
Muhammad Rizal
Keyword(s):  
Sperm Concentration ◽  
Egg Yolk ◽  
Control Group ◽  
Spermatozoa Motility ◽  
The Mean ◽  
Epididymal Spermatozoa ◽  
Live Sperm

This research was conducted to investigate the effect of various concentrations of lactose supplementation in Tris extender for maintaining the quality of Etawa crossbreed goat epididymal spermatozoa stored at 3-5° C. Semen in the control group was diluted with a tris extender containing 20% egg yolk without lactose. Semen in the test groups was diluted with a tris extender containing 20% egg yolk and added with 0.3% (0.3 g per 100 mL extender) and 0.6% lactose for group TL1 and TL2, respectively. Parameters evaluated of the fresh epididymal spermatozoa were motility, concentration, percentage of live, and abnormality of spermatozoa, while for diluted-spermatozoa were motility and percentage of live spermatozoa. Spermatozoa observation was conducted until it reaches 40% motility. The results showed that the mean percentage of motility, live sperm, concentration, and abnormality of epididymal spermatozoa were 70%; 81%; 3,220x106 cells/mL; and 4.30%, respectively in all group. After dilution, the percentage of motility and live spermatozoa were also 70% and 81.00±1.58%, respectively in all groups. The decreasing of spermatozoa motility was observed on day 4 of storage, in which percentage of spermatozoa motility in control group (40.00±0.00%) was significantly lower (P<0.05) than those in TL1 (44.00±2.24%) and TL2(45.00±0.00%) groups. Percentage of live spermatozoa in control (63.20±2.68%) was not significantly different (P>0.05) than TL1(65.40±1.95%) and TL2 (65.60±1.95%). In conclusion, the supplementation of lactose into Tris extender could maintain the epididymal spermatozoa of Etawa crossbreed for 3 days of storage at 3-5° C.

scholarly journals Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing

2012 ◽  
Vol 55 (3) ◽  
pp. 207-218 ◽  
Author(s):  
J. Beran ◽  
L. Stádník ◽  
J. Bezdíček ◽  
F. Louda ◽  
J. Čítek ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Volume Density ◽  
Data Set ◽  
Holstein Bulls ◽  
Live Sperm

Abstract. The objectives were to evaluate the effects of sire and content of fresh or ionised egg yolk in extenders on sperm motility and share of live and dead sperm in collected ejaculate, in thawed artificial insemination (AI) doses, and during thermodynamic testing compared to extenders not containing egg yolk. Ejaculates were collected once a week from 4 Holstein bulls. Each of the 20 ejaculate samples from each bull was diluted with 4 different extenders. AndroMed and Bioxcell (no egg yolk) and Triladyl and Optidyl (fresh, ionised egg yolk) were used. A total of 640 AI doses were analysed. The volume of samples, sperm concentration, and percentage of motile spermatozoa were evaluated after collection, as was sperm motility after thawing of AI doses and during thermodynamic testing. Percentages of live and dead sperm were also evaluated. The data set was analysed using SAS/STAT 9.1 (SAS Institute Inc., Cary, NC, USA). The results confirmed significant (P<0.05–0.01) between-sire differences in the volume, density, and activity of sperm as well as in share of live and dead sperm after collection; in decline of sperm motility in the fresh ejaculate, after thawing, and during the entire thermodynamic test, as well as in the share of live and dead sperm after thawing. The extenders ranked by sperm motility are: Optidyl, Triladyl, AndroMed, and Bioxcell, demonstrating the higher quality of AI doses produced using egg yolk extenders. Differences in sperm motility were significant (P<0.05–0.01) during the entirety of thermodynamic testing. Egg yolk extenders had a significantly (P<0.05–0.01) higher percentages of live sperm after thawing.

A Comparative Study of Semen Characteristics and Testosterone Concentration in Ram Breeds of Saudi Arabia

2020 ◽  
Author(s):  
A. A. Al-kawmani ◽  
M. A. Farah ◽  
S. Nahdi ◽  
A. H. Harrath ◽  
H. Al-Jawdah
Keyword(s):  
Saudi Arabia ◽  
Age Groups ◽  
Sperm Concentration ◽  
Breeding Programs ◽  
Testosterone Concentration ◽  
Semen Characteristics ◽  
The Mean ◽  
Selection For ◽  
Live Sperm ◽  
Sperm Output

The goal of this study was to evaluate and compare the semen characteristics, and testosterone concentration for three breeds raised under hot, desert conditions in Saudi Arabia. A total of 150 rams (Najdi, Naemi, and Harri) were used, aged 12, 18, 24, 30 and 36 months (ten rams from each breed in each age group). Body weight and testicular parameters were measured. In addition, testosterone concentration (TC), ejaculate volume (EV), sperm concentration (SC), total sperm output per ejaculate (TSO), live sperm percentage (LSP), sperm progressive motility (SPM) and sperm mass motility (SMM). The results show that TC and EV differed significantly (P less than 0.01) between breeds. At 36 months, semen characteristics (TSO, SMM and SPM) differed significantly among breeds. Naemi rams were superior to Najdi and Harri in terms of the mean live sperm percentage (LSP) for all age groups. These results may assist in ram selection for breeding programs based on high reproductive performance.

scholarly journals Effect of preservation time on the quality of frozen semen in indigenous rams

2015 ◽  
Vol 44 (1) ◽  
pp. 10-15 ◽  
Author(s):  
BBA Mahmuda ◽  
Azizun Nesa ◽  
BF Zohara ◽  
MGS Alam ◽  
FY Bari
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Volume Density ◽  
Normal Morphology ◽  
Mean Values ◽  
Frozen Semen ◽  
Significant Difference ◽  
The Mean ◽  
Fresh Semen

The study was carried out to observe the effects of preservation time on the quality of frozen semen of indigenous rams. Semen was collected using AV once a week from 4 rams. Tris based with 10% egg yolk and 7% glycerol extender was used to extend and freezing the semen. Fresh semen was evaluated for volume, density, mass motility and concentration, and mean values were observed as 0.8±0.2ml, 3.0±0.3, 3.2±0.7, 3.9±0.7×109/ml, respectively. Significant difference (p<0.05) was found for all the parameters among the rams. Mean values of motility, viability and normal morphology percentages were 83.3±4.3%, 88.2±4.4%, 84.2±3.5% in fresh semen while those of chilled semen at 40C were 74.7±2.3, 78.8±4.9 and 79.2±2.9%, respectively. For all the parameters, significant (p<0.05) difference was found among the rams. Frozen sperm motility was observed after thawing at 39-400C for 14-15 seconds. The mean motility, viability and normal morphology percentages after freezing for 24hrs, 7, 15 and 30 days of duration were 39.8±3.1, 41.1±4.3, 40.1±4.1 and 39.4±2.9%; 44.5±2.5, 45.3±2.8, 44.6±2.8 and 43.9±2.8%; 71.0±2.0, 71.7±1.5, 70.7±1.7 and 70.3±1.8%, respectively and values did not decrease significantly (p>0.05) with the increasing time of preservation. Non significantly decrease of the semen quality with advance of preservation time indicates the suitability of the protocol used for freezing of indigenous ram semen in Bangladesh.DOI: http://dx.doi.org/10.3329/bjas.v44i1.23113            Bang. J. Anim. Sci. 2014. 44 (1): 10-15

ASSESSMENT OF BOVINE SEMEN QUALITY BY RESAZURIN REDUCTION TEST

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Umesh Kumar ◽  
Prasanna Pati ◽  
A.P. Gawande
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Mean Value ◽  
Bovine Semen ◽  
Reduction Test ◽  
The Mean ◽  
Acrosomal Integrity

A study was conducted to assess the semen quality using Resazurin Reduction Test (RRT) in Jersey and Jersey cross bulls. A total 36 fresh ejaculates were collected for evaluation RRT, sperm motility, acrosomal integrity and sperm concentration. The mean value of RRT in bulls was 15.77±0.85 minutes.Statistical analysis revealed that RRT was highly significantly (p<0.01) and positively correlated with sperm concentration (r=0.769), sperm motility(r=0.858) and acrosomal integrity (r=0.515), hence it may be helpful in evaluating the quality of semen in bulls.

Effect of different extenders for preservation of ram semen at 4°C

2016 ◽  
Author(s):  
Haneef A. Rather ◽  
Rafiqul Islam ◽  
Asloob A. Malik ◽  
Farooz A. Lone ◽  
Mohamad Naiem Banday
Keyword(s):  
Citric Acid ◽  
Sperm Motility ◽  
Egg Yolk ◽  
Morphological Abnormalities ◽  
Progressive Motility ◽  
Hypoosmotic Swelling Test ◽  
Live Sperm

The aim of this study was to investigate the effects of different extenders viz. Tris citric acid fructose egg yolk (TCFEY), Tris citric acid glucose egg yolk (TCGEY), Egg yolk citrate fructose (EYCF) and Egg yolk citrate glucose (EYCG) on the quality of ram spermatozoa during preservation at 4°C. Semen samples showing more than 3+ mass motility and 70% progressive motility were pooled and subsequently divided into four aliquots. Each aliquot was extended separately in four different extenders viz. TCFEY, TCGEY, EYCF and EYCG and stored at 4°C up to 72h. The quality of spermatozoa on the basis of percentage of sperm motility, live sperm, morphological abnormalities, intact acrosome and hypoosmotic swelling test (HOST) reacted spermatozoa was evaluated immediately after extension in particular extenders (0 h), 24 h, 48 h and 72 h after preservation at 4°C. The percent sperm motility was significantly (P<0.01) higher for TCFEY and TCGEY than EYCF and EYCG at 72 h of preservation at 4°C. The percent HOST reacted spermatozoa and intact acrosomes were significantly (P<0.01) higher and morphological abnormalities were significantly (P<0.01) lower for Tris based fructose extender than other three extenders at 72 h at 4°C. In conclusion, Tris citric acid fructose egg yolk (TCFEY) was found the best in maintaining the quality of ejaculated ram spermatozoa during preservation for 72 h at 4°C. 

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