ASSESSMENT OF BOVINE SEMEN QUALITY BY RESAZURIN REDUCTION TEST

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Umesh Kumar ◽  
Prasanna Pati ◽  
A.P. Gawande
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Mean Value ◽  
Bovine Semen ◽  
Reduction Test ◽  
The Mean ◽  
Acrosomal Integrity

A study was conducted to assess the semen quality using Resazurin Reduction Test (RRT) in Jersey and Jersey cross bulls. A total 36 fresh ejaculates were collected for evaluation RRT, sperm motility, acrosomal integrity and sperm concentration. The mean value of RRT in bulls was 15.77±0.85 minutes.Statistical analysis revealed that RRT was highly significantly (p<0.01) and positively correlated with sperm concentration (r=0.769), sperm motility(r=0.858) and acrosomal integrity (r=0.515), hence it may be helpful in evaluating the quality of semen in bulls.

Fresh and Post-Thaw Seminal Attributes of Jafarabadi Buffalo Bulls

2020 ◽  
Vol 15 (03) ◽  
pp. 45-48
Author(s):  
K. H. Parmar ◽  
FS Kavani ◽  
TK Patbandha ◽  
SS Parikh
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Conception Rate ◽  
Mean Values ◽  
Abnormal Sperm ◽  
The Mean ◽  
Live Sperm ◽  
One Year ◽  
Acrosomal Integrity

The study was conducted on four Jafarabadi breeding bulls, 5-6 years old to know the fresh and post-thawed seminal characteristics based on total of 192 semen ejaculates evaluated and cryopreserved over one year period. The mean values of fresh neat seminal characteristics of Jafarabadi bulls, viz., ejaculate volume (ml), colour/density (score), sperm concentration (million/ml), mass activity (score), initial motility (%), live sperm (%), abnormal sperm (%), HOS reactive sperm (%) and acrosomal integrity (%) were 5.19±0.18, 2.38±0.10, 1253.36±24.75, 3.73±0.05, 80.31±0.05, 86.20±0.64, 5.00±0.40, 85.75±0.43 and 93.56±0.56, respectively, whereas the mean post-thawed sperm characteristics, viz., progressive sperm motility, live sperm (%), abnormal sperm (%), HOS reactive sperm (%), acrosomal integrity (%) and first insemination conception rate (%) observed were 57.60±0.36, 66.34±0.53, 8.85±0.33, 56.97±0.46, 75.26±0.17 and 44.63±0.14, respectively. The semen quality of fresh and post-thawed samples observed was within normal limit for use in breeding program with satisfactory first insemination conception rate.

scholarly journals Spectrophotometric Analysis of the Resazurin Reduction Test as a Tool for Assessing Canine Semen Quality

2013 ◽  
Vol 57 (2) ◽  
pp. 281-285 ◽  
Author(s):  
Rafał Strzeżek ◽  
Krystyna Filipowicz ◽  
Marta Stańczak ◽  
Władysław Kordan
Keyword(s):  
Sperm Motility ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Spectrophotometric Analysis ◽  
Normal Morphology ◽  
Additional Tool ◽  
Reduction Test ◽  
Highly Correlated

Abstract The resazurin reduction test (RRT) was subjected to spectrophotometric analysis to evaluate the quality of canine semen. Twenty four samples of canine semen were analysed. The absorption peaks for resazurin and resorufin were determined at 615 and 580 nm, respectively. The RRT ratio (RRTsperm-the ratio for samples containing spermatozoa, RRTplasma-the ratio for samples containing seminal plasma) was calculated by dividing the absorbance at 580 nm by the absorbance at 615 nm. Spearman’s correlation test was used to determine the significance of correlations between the analysed sperm parameters and the results of the resazurin reduction assay. The RRT ratio was highly correlated with sperm motility (r=0.68, P<0.01), progressive sperm motility (r=0.61, P<0.01), the subpopulation of cells with rapid velocity (r=0.72, P<0.01), and the subpopulation of cells with medium velocity (r= -0.54, P<0.05). A negative correlation was observed between the reducing capacity of seminal plasma vs. sperm with plasma membrane integrity (r= -0.60, P<0.01) and sperm with normal morphology (r= -0.58, P<0.01). The RRT test can be used as an additional tool for evaluation of the quality of canine semen.

23 Sperm quality of Pure Spanish stallions is affected by inbreeding coefficient and age

2020 ◽  
Vol 32 (2) ◽  
pp. 137
Author(s):  
Y. Pirosanto ◽  
M. Valera ◽  
A. Molina ◽  
J. Dorado ◽  
S. Demyda-Peyrás
Keyword(s):  
Sperm Motility ◽  
Inbreeding Depression ◽  
Negative Correlation ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Inbreeding Coefficient ◽  
Semen Parameters ◽  
Computer Assisted

Inbreeding depression, a genetic condition produced by the mating of close-related individuals, has been associated with a reduction of fertility in several species. However, a loss in sperm quality was also associated with age. In horses, the few existing reports have described a tendency of both parameters to produce a negative effect on sperm quality. However, those reports were performed using a subjective evaluation of sperm motility. In the present study, a total of 692 ejaculates from 86 Pure Spanish stallions (PRE), aged between 3 and 22 years, were evaluated using a computer-assisted methodology to determine the effect of inbreeding in four semen parameters: free-gel volume (V), sperm concentration (C, by haemocytometer), and total (TM) and progressive (PM) sperm motility (by Spermvision sperm class analyser; Minitube). The inbreeding coefficient (F) was estimated using 300 000 PRE pedigree records approximately (minimum pedigree depth, eight equivalent complete generations; range, between 1 and 30.1%). Stallion, age, ejaculate, and season of semen collection were the variables included in the statistical model (general linear model), with ejaculate and season being the variables with a major effect (by variance components analysis). Our results showed that sperm concentration (r=−0.18; P&lt;0.0001) and volume (to a lesser extent) were reduced with advancing age, both showing a major decline after 15 years of age. To the contrary, sperm motility was not affected by age of the stallion. We also found a negative correlation between the inbreeding coefficient and ejaculate volume (r=−0.14; P&lt;0.001), with a marked decrease seen when F was between 7 and 20%. Also, a negative correlation was observed in PM (r=−0.08; P&lt;0.05), although to a lower extent. Conversely, C and TM were not affected by inbreeding depression (P&gt;0.05). In conclusion, our results demonstrated that high levels of inbreeding can compromise severely the sperm quality of the PRE stallion, which, subsequently, may have a negative influence on fertility. Ongoing studies using genomic data will help to detect genetic variants associated with stallion semen quality and how it is influenced by inbreeding in specific genomic regions.

scholarly journals The quality of buck semen after feed additive minoxvit administration

2021 ◽  
Vol 19 (1) ◽  
pp. 87
Author(s):  
Anita Hafid ◽  
Riasari Gail Sianturi ◽  
Diana Andrianita Kusumaningrum ◽  
Yeni Widiawati ◽  
Anneke Anggraeni ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Positive Influence ◽  
Feed Additive ◽  
Concentration Data ◽  
Randomized Design ◽  
Microscopic Evaluation

<p class="MDPI17abstract"><strong>Objective: </strong>Reproduction and nutritional status is closely related. Nutritional deficiency or insufficiency directly affects reproductive efficiency. Deficiency of nutrition could affect the sperm quality and the ability to fertilize. The objective of this study was to evaluate the effect of feed additive (Minoxvit) on semen quality of buck.</p><p class="MDPI17abstract"><strong>Methods: </strong>This study used 6 sexually mature bucks, aged 1.5 years old. The bucks were fed daily with 3 kg of freshly chopped king grass, 1 kg of Legume (<em>Calliandra</em> sp.), and 500 g of concentrate. Three bucks were given Minoxvit by 1.25 g/day in the concentrate, while three other bucks were considered as control. Semen was evaluated once a week for 49 days. Semen was evaluated macroscopically and microscopically. The macroscopic evaluation consisted of volume, color, and consistency of semen, while microscopic evaluation consisted of mass motility, sperm motility, viability, and sperm concentration. Data were analyzed using Completely Randomized Design with Tukey test.<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The result showed significantly different (<em>P</em>&lt;0,05) in which bucks semen in Minoxvit addition group had higher semen volume (0.75 ml vs 0.54 ml), mass motility (3.32 vs 2.67), sperm motility (70% vs 58 %), sperm viability (86.67% vs 79.19%), and sperm consentration (2,6x10<sup>9</sup> mL vs 1,7x10<sup>9</sup> mL).<strong></strong></p><p><strong>Conclusions: </strong>This study concludes that the addition of Minoxvit has a positive influence on the quality of buck sperms providing volume, mass motility, individual motility, viability, and concentration of the sperm.</p>

scholarly journals Influences of bull age and season on sperm motility, sperm concentration, and ejaculate volume of Ongole Grade cattle in Singosari National Artificial Insemination Center

2020 ◽  
Vol 45 (4) ◽  
pp. 261-267
Author(s):  
S. Suyadi ◽  
B. Purwantara ◽  
A. Furqon ◽  
W. A. Septian ◽  
I. Novianti ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Dry Season ◽  
Production Data ◽  
Wet Season

Ongole Grade cattle is one of local cattle in Indonesia.This study was aimed to analyze the effect of bull age and season on semen quality of Ongole Grade cattles macroscopically (sperm motility, and sperm concentration) and microscopically (semen ejaculate volume) to support the policy and management on improvement of semen production. Data were collected from 533 ejaculates of 10 bulls aged 3, 4, and 5 yr at Singosari National Artificial Insemination Center(SNAIC). The 4-year-old bulls hadhighestsperm motiliy and semen ejaculate volume. The lowest ejaculate volume was reported in Ongole Gradecattlesaged 3 yr. Sperm motility and concentrationweresignificantly higher (P<0.01) in wet season than dry season. In conclusion, the bull age and season should be considered by Singosari National Artificial Insemination Centerwhen collecting the semen, especially on bulls aged 3 yr old and in dry season.

scholarly journals Addition of chlorogenic acid and caffeine during the processing of cooled boar semen

2019 ◽  
Vol 71 (2) ◽  
pp. 489-499
Author(s):  
B.A. Pereira ◽  
L.G.P. Rocha ◽  
M.C. Teles ◽  
W.E. Silva ◽  
J.A. Barbosa ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Sperm Motility ◽  
Semen Quality ◽  
Semen Parameters ◽  
Mitochondrial Activity ◽  
Boar Semen ◽  
Acrosome Integrity ◽  
Acrosomal Integrity

ABSTRACT A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P <0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P <0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P<0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P <0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P <0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.

scholarly journals Boar semen quality of the Přeštice black-pied breed (Short Communication)

2011 ◽  
Vol 54 (4) ◽  
pp. 374-380 ◽  
Author(s):  
S. Frydrychová ◽  
A. Lustyková ◽  
J. Lipenský ◽  
M. Rozkot
Keyword(s):  
Dilution Rate ◽  
Sperm Motility ◽  
Short Communication ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Test Period ◽  
Boar Semen ◽  
Natural Mating

Abstract. The objective of this study was to examine semen quality parameters for the Přeštice black-pied breed over a test period of 8 years while considering the potential effects of collection month and boar age. Ninety-nine ejaculates were collected using the gloved-hand technique from healthy and fertile mature boars from selected farms. Ejaculate volumes were relatively low because the boars were accustomed to natural mating. Sperm motility, sperm concentration, percentage of morphologically abnormal spermatozoa (MAS), and sperm motility after 24 h of storage in Androhep extender (dilution rate 1:1.5) were assessed. Significant differences were found in sperm concentration and MAS rate in relation to collection months and boar age in the monitored years (P<0.05). A tendency for MAS to increase with monitored years was observed. Significant differences in sperm motility and motility after 24 h of storage were only observed in relation to collection months (P<0.05). Results of this study detected effects due to collection month and boar age on boar semen quality during the monitored years.

scholarly journals THE QUALITY OF BOAR FROZEN SEMEN DILUTED IN BTS® AND MII® WITH DIFFERENT CRYOPROTECTANT SUPPLEMENTED WITH SODIUM DODECYL SULPHATE

2017 ◽  
Vol 11 (1) ◽  
pp. 6-10
Author(s):  
Nancy Diana Frederika Katerina Foeh ◽  
Raden Iis Arifiantini ◽  
Tuty Laswardi Yusuf
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Sodium Dodecyl Sulphate ◽  
Semen Quality ◽  
Sodium Dodecyl ◽  
Sperm Concentration ◽  
Sperm Abnormalities ◽  
Semen Collection ◽  
Frozen Semen

This research was aimed to study the effect of administration of glycerol and dimetilacetamida (DMA) in BTS® and MIII®extender supplemented with sodium dodecyl sulphate (SDS) on boar frozen semen. A number of four boars were used in this study for semen collection (n=20). The collected semen was evaluated both macroscopically and microscopically. In this study, only the semen that demonstrated>70% sperm motility, >200.106/mL sperm concentration, and<20% sperm abnormalities were used and divided into eight tubes. A number of 4 tubes were diluted with 5 mL of BTS, and the rest with 5 mL MIII. The sampel was stored at 20-22° C for 2 hours, followed by centrifugation for 15 minutes (at 2000 rpm), and taken of pellet with 1 ml supernatant. The pellet that was resulted from centrifugation using BTS, then re-diluted with BTS-glycerol 5% (BTSG), BTS DMA 5% (BTSD), BTS-glycerol 5% and SDS (BTSG-S), BTS-DMA 5% and SDS (BTSD-S). Four other pellets that were centrifuged with MIII also re-diluted with MIII-glycerol 5% (MIIIG), MIII-DMA 5% (MIIID), MIII-glycerol 5% and SDS (MIIIG-S), MIII-DMA 5% and SDS (MIIID-S). Next, all of diluted semen were inserted into 0.5 mL straw and equilibrated for 2 hours (4° C), then frozen and stored in liquid nitrogen. The evaluation of frozen semen quality was conducted at 24 hours after frozen. The result of this study showed that post-thawing motility of spermatozoa in BTSD-S (40.17±0.2%) was found higher (P<0.05) compared to seven other dilution processes. Therefore, it is concluded that the concentration of 5% DMA that supplemented with SDS in BTS dilution much better for maintaining boars frozen semen quality.

Characteristics and freezability of Assam Hill goat semen

2017 ◽  
Author(s):  
S. Deori ◽  
B. C. Deka ◽  
R. K. Biswas ◽  
N. Nahardeka ◽  
A. Arangasamy ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Mean Values ◽  
Acceptable Quality ◽  
Sperm Abnormality ◽  
Semen Characteristics ◽  
The Mean ◽  
Live Sperm

Assam Hill goat (AHG) is an important goat germplasm found in Assam and its adjoining areas of India. The study was designed with an objective to study the semen characteristics and freezability of AHG buck semen using Tris -Egg yolk-Citrate-Fructose diluent. The mean values of fresh semen characteristics in AHG bucks viz., ejaculate volume (ml), initial sperm motility (%), sperm concentration (x106/ml), live sperm (%), sperm abnormality (%), HOST-reacted sperm (%) and intact acrosome (%) recorded were 0.39 ± 0.01, 77.97 ± 0.73, 3201.00 ± 143.78, 83.02 ± 0.65, 7.66 ± 0.73, 66.95 ± 0.74 and 93.34 ± 0.51, respectively. Mean values for post-thaw semen characteristics i.e., sperm motility (%), live sperm (%), HOST-reacted sperm (%) and intact acrosome (%) were 55.39 ± 0.97, 71.01 ± 0.78, 54.77 ± 0.55 and 82.16 ± 0.43, respectively. It can be concluded that AHG bucks donate acceptable quality of semen which can be frozen successfully in Tris-Egg yolk-Citrate-Fructose diluents for using in Artificial Insemination.

scholarly journals Bovine semen thermoresistance tests and their correlation with pregnancy rates after fixed-time artificial insemination

2019 ◽  
Vol 71 (6) ◽  
pp. 2085-2092
Author(s):  
R. Talini ◽  
L.E. Kozicki ◽  
F.R. Gaievski ◽  
G. Polo ◽  
L.G.F. Lima ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Sperm Motility ◽  
Negative Correlation ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Rapid Test ◽  
Significant Negative Correlation ◽  
Fixed Time ◽  
Bovine Semen ◽  
The Mean

ABSTRACT The objective of the study was to determine whether or not there is a correlation between thermoresistance tests (TT) after semen thawing and pregnancy rate (PR) after fixed-time artificial insemination (FTAI). Four different TT were performed on ten samples used for AI; a rapid test (RTT) (30min / 46°C) and three slow tests (STT): STT1 (60min/38°C), STT2 (180min/38°C), and STT3 (300min/38°C). Two hundred and fifteen multiparous crossbred cows were submitted to FTAI under the following protocol: on day zero (d0) the animals received a P4 device +EB; on d7 PGF2α; on d8 P4 was removed and eCG+EC were administered; IATF was performed on d10. Three gestational diagnoses (G D) were performed on d40, d70 and d120. The mean sperm motility (%) in RTT and STTs were 19.84±6.13, 28.55±10.48, 17.62±5.87 and 8.63±3.46, respectively, and TP in the three DG 61.86%, 57.67%, and 55.81%, respectively. Through Person test a significant negative correlation (P< 0.05) was found between STT2 and PR at 60 days (r= -0.644) and between STT3 and all TPs (r= -0.774, -0.752, 0.748). It was concluded that TT parameters are not able to determine correlation between semen quality and TP.

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