A Comparative Study of Semen Characteristics and Testosterone Concentration in Ram Breeds of Saudi Arabia

2020 ◽  
Author(s):  
A. A. Al-kawmani ◽  
M. A. Farah ◽  
S. Nahdi ◽  
A. H. Harrath ◽  
H. Al-Jawdah
Keyword(s):  
Saudi Arabia ◽  
Age Groups ◽  
Sperm Concentration ◽  
Breeding Programs ◽  
Testosterone Concentration ◽  
Semen Characteristics ◽  
The Mean ◽  
Selection For ◽  
Live Sperm ◽  
Sperm Output

The goal of this study was to evaluate and compare the semen characteristics, and testosterone concentration for three breeds raised under hot, desert conditions in Saudi Arabia. A total of 150 rams (Najdi, Naemi, and Harri) were used, aged 12, 18, 24, 30 and 36 months (ten rams from each breed in each age group). Body weight and testicular parameters were measured. In addition, testosterone concentration (TC), ejaculate volume (EV), sperm concentration (SC), total sperm output per ejaculate (TSO), live sperm percentage (LSP), sperm progressive motility (SPM) and sperm mass motility (SMM). The results show that TC and EV differed significantly (P less than 0.01) between breeds. At 36 months, semen characteristics (TSO, SMM and SPM) differed significantly among breeds. Naemi rams were superior to Najdi and Harri in terms of the mean live sperm percentage (LSP) for all age groups. These results may assist in ram selection for breeding programs based on high reproductive performance.

Monthly variations in some reproductive parameters of Barki and Awassi rams throughout 1 year under subtropical conditions 1. Semen characteristics and hormonal levels

2000 ◽  
Vol 71 (2) ◽  
pp. 317-324 ◽  
Author(s):  
T. A. Taha ◽  
E. I. Abdel-Gawad ◽  
M. A. Ayoub
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Early Spring ◽  
Serum Testosterone Level ◽  
Four Seasons ◽  
Semen Characteristics ◽  
Live Sperm ◽  
Sperm Output ◽  
Monthly Variations

AbstractFifteen sexually mature rams, five each of Barki, Awassi (I, imported from Syria) and Awassi (LB, locally born in Egypt) were used in this study. Semen was collected monthly from rams for a period of 12 months to study semen characteristics. In addition, blood samples were collected from rams during the four seasons of the year to determine serum triiodothyronine (T3) and testosterone hormones. Results showed that Barki and Awassi (I and LB) rams are continuous breeders as they show sexual desire and produce semen all the year round. However, monthly variations in semen quality were detected. Relative testes volume, ejaculate volume, sperm concentration, total sperm output, sperm motility, percentage of live sperm and serum testosterone level were higher during summer months than at other months of the year. Serum T3 was significantly higher in winter and spring than that observed in summer and autumn. In addition, percentages of dead spermatozoa were higher in winter and early spring than in autumn and summer. Furthermore, summer months showed moderate percentages of abnormal spermatozoa and spermatozoa without intact acrosomes. These results indicate that ram semen quality in the subtropics may be better during summer months than during other months of the year.

Characteristics and freezability of Assam Hill goat semen

2017 ◽  
Author(s):  
S. Deori ◽  
B. C. Deka ◽  
R. K. Biswas ◽  
N. Nahardeka ◽  
A. Arangasamy ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Mean Values ◽  
Acceptable Quality ◽  
Sperm Abnormality ◽  
Semen Characteristics ◽  
The Mean ◽  
Live Sperm

Assam Hill goat (AHG) is an important goat germplasm found in Assam and its adjoining areas of India. The study was designed with an objective to study the semen characteristics and freezability of AHG buck semen using Tris -Egg yolk-Citrate-Fructose diluent. The mean values of fresh semen characteristics in AHG bucks viz., ejaculate volume (ml), initial sperm motility (%), sperm concentration (x106/ml), live sperm (%), sperm abnormality (%), HOST-reacted sperm (%) and intact acrosome (%) recorded were 0.39 ± 0.01, 77.97 ± 0.73, 3201.00 ± 143.78, 83.02 ± 0.65, 7.66 ± 0.73, 66.95 ± 0.74 and 93.34 ± 0.51, respectively. Mean values for post-thaw semen characteristics i.e., sperm motility (%), live sperm (%), HOST-reacted sperm (%) and intact acrosome (%) were 55.39 ± 0.97, 71.01 ± 0.78, 54.77 ± 0.55 and 82.16 ± 0.43, respectively. It can be concluded that AHG bucks donate acceptable quality of semen which can be frozen successfully in Tris-Egg yolk-Citrate-Fructose diluents for using in Artificial Insemination.

scholarly journals Raw Semen Characteristics of Three Different Indonesian Local Roosters

2016 ◽  
Vol 18 (3) ◽  
pp. 165
Author(s):  
Emilia Kamung Hambu ◽  
Raden Iis Arifiantini ◽  
Bambang Purwantara ◽  
Sri Darwati
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Mass Movement ◽  
Sperm Concentration ◽  
Semen Volume ◽  
Sperm Abnormality ◽  
Microscopic Evaluation ◽  
Semen Characteristics ◽  
Live Sperm ◽  
Better Than

Indonesia has agreat variety of roosters, either indigenous type as well as exotic and cross breed. The purpose of this experiment was to study the characteristics of semen from three types of Indonesian local roosters such as Merawang, Kampung and crosses Sentul Kampung with Kedu (SK Kedu). A total of 15 roosters consist of  Merawang roosters,  Kampung, and SK Kedu roosters were 5 each. The semen was collected 3 times a week by dorso-abdominal and cloaca massage method. The parameters evaluation was macroscopic characteristics consist of volume, color, consistency, and pH. Microscopic evaluation of semen such as a mass movement, sperm motility, live sperm, sperm abnormality and sperm concentration. Results of this experiment showed that semen volume of  Merawang  (0.40±0.26 mL) was higher (p<0.05) compare to Kampung (0.24±0.12 mL) or  SK Kedu (0.16±0.10 mL) but no difference on semen color, consistency and semen pH. There were no difference in the mass movement, sperm motility and live sperm as well as on sperm abnormality among three types of roosters.  Sperm  concentration of Merawang (4490 million mL-1) was significantly higher than Kampung (3245 million mL-1) and the SK Kedu roosters (3751 million mL-1). Its was conclude that Merawang roosters had good semen quality better than Kampung and SK Kedu roosters

116. EFFECTS OF SUPPLEMENTAL VITAMIN ON THE SEMEN CHARACTERISTICS OF MARKHOZ GOATS DURING NON-BREEDING SEASON

2009 ◽  
Vol 21 (9) ◽  
pp. 35
Author(s):  
A. Towhidi ◽  
A. Farshad ◽  
M. Dolatpanah ◽  
R. Salehi
Keyword(s):  
Vitamin E ◽  
Breeding Season ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Progressive Motility ◽  
Semen Volume ◽  
Semen Characteristics ◽  
Supplemental Vitamin ◽  
Diet Control ◽  
Live Sperm

The aim of the study was to investigate the effect of dietary supplementation of vitamin E on the semen characteristics of the Markhoz goats. Eighth bucks were randomly allocated into two groups, and received two different diets: unsupplemented diet (control) and supplemented diet with vitamin E (0.30 g/Kg DM). All experimental diets were formulated according to AFRC 1998. Semen was collected at 14-days intervals from June 17, 2006 to September 2, 2006 (non-breeding season) using artificial vagina. Semen characteristics were evaluated including semen volume, sperm concentration, live sperm percentage, percentage of motility and progressive motility. This characteristics were evaluated at two weeks intervals the trial. The total number of spermatozoa per ejaculation was calculated by multiplication of the semen volume with sperm concentration. Sperm motility was also analyzed by placing a sample on pre-warmed (37 º C) microscope slide covered with a cover slip, and examined under a high power microscope at a magnification × 200. Data was analyzed using proc MIXED in SAS program. Significant effect (P≤0.05) of the week (sampling time) was observed for all the parameters except for semen volume. Vitamin E supplementation significantly improved (P≤0.05) total number (Control: 263.7 ×107±17.506 vs. Vit E: 320.95 ×107±17.506) and sperm concentration (Control: 301.79 ×107±13.657 vs. Vit E: 386.57 ×107± 13.657), motility (Control: 77.27% ±0.89 vs. Vit E: 82.6% ±0.89) and progressive motility (Control: 4.208 ±0.138 vs. Vit E: 4.229 ±0.138), percentage of viability (Control: 80.57% ±0.89 vs. Vit E: 85.9% ±0.89). The results suggested that the supplemental Vitamin E may improve the semen quality and fertility in the Markhoz goats.

scholarly journals The effect of freezing on cryosurvival of yak sperm

2018 ◽  
Vol 15 (2) ◽  
pp. 215-218
Author(s):  
S Deori
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Mean Values ◽  
Frozen Semen ◽  
Sperm Abnormality ◽  
Semen Characteristics ◽  
Live Sperm

A study was carried out to study the effect of freezing on cryosurvival of yak semen. Artificial insemination in yak is still in infancy. Semen cryopreservation and use of artificial insemination can be applied in yak husbandry for conservation and rapid multiplication of superior germplasm. Semen was collected from four adult yak bulls using artificial vagina method managed under uniform conditions. A total of 40 ejaculates comprising of 10 ejaculates each bull were collected following twice a week schedule and evaluated for fresh semen characteristics. The fresh yak semen characteristics viz. ejaculate volume (ml), mass activity (0-4), initial sperm motility (%), sperm concentration (x 106/ml), live sperm (%), sperm abnormality (%) and intact acrosome (%) were 3.10 ± 0.18, 3.53 ± 0.96, 83.89 ± 2.87, 1180.22 ± 42.32, 77.63 ± 4.23, 8.45 ± 3.33 and 93.61 ± 3.78 respectively. The ejaculates were diluted (1:10) with Tris extender consisting of 6.4 ml glycerol and 20 ml of fresh egg yolk. Straws were equilibrated at 5°C for 4 hours followed by exposure to liquid nitrogen vapour for 10 minutes and finally transferred to liquid nitrogen container for storage. The cryosurvival rate was studied after 7 days of storage in liquid nitrogen. The frozen semen was thawed in warm water (37°C) for 30 seconds for evaluation. Mean values of postthaw sperm motility (%), live sperm (%) and intact acrosome (%) in yaks were 55.67 ± 4.67, 65.62 ± 3.23 and 89.26 ± 3.67 respectively. In conclusion, yak semen has a better cryosurvival while freezing in tris extender with 6.4 per cent glycerol and 20 per cent egg yolk following an equilibration period of 4h.SAARC J. Agri., 15(2): 215-218 (2017)

Test-weight and weathering of spring wheat

2000 ◽  
Vol 80 (4) ◽  
pp. 677-685 ◽  
Author(s):  
Y. T. Gan ◽  
T. N. McCaig ◽  
P. Clarke ◽  
R. M. DePauw ◽  
J. M. Clarke ◽  
...  
Keyword(s):  
Weight Loss ◽  
Historical Data ◽  
Triticum Turgidum ◽  
Genotypic Variation ◽  
Screening Strategy ◽  
Test Weight ◽  
Genotypic Differences ◽  
Breeding Programs ◽  
The Mean ◽  
Selection For

Wet weather often delays harvest and results in a grade reduction of wheat because of a decrease in test-weight, an important grading factor in Canada. The objectives of this study were to assess the effect of delayed harvest on test-weight loss of 14 Canadian wheat cultivars representing three different classes, and to develop a screening strategy for retention of test-weight for breeding programs. Non-weathered test-weight (NWTWt), weathered test-weight (WTWt), and test-weight loss (TWtLoss; i.e. NWTWt – WTWt) in the field, averaged over five field locations and 2 yr were similar within the CPS, CWAD and CWRS wheat classes, although there were genotypic differences for all three variables. Because test-weight requirements for the top grades are higher in the CWAD class than in other classes, durum cultivars would be more susceptible to downgrading during wet harvests. Historical data from the Durum Wheat Co-operative Test also suggests that, since 1950, the mean test-weight of the genetic lines has decreased by 3.7 kg hL−1, and is now close to the minimum for grade #1 in the CWAD class. Most of the decrease in test-weight observed over several weeks in the field could be simulated by a single 5 – 10 min soaking of non-weathered seed in the laboratory. Linear regression analyses of both field and laboratory samples indicated that 90% of the genotypic variation in weathered test-weight could be attributed to differences in the NWTWt. These results suggest that the screening strategy for retaining test-weight should focus on selection for increased NWTWt. This is much simpler than screening for high WTWt or low TWtLoss, which requires soaking of the seed or field weathering. Key words: Triticum aestivum, Triticum turgidum, quality

Semen and testis characteristics and sexual behaviour of boars

1959 ◽  
Vol 53 (3) ◽  
pp. 313-326 ◽  
Author(s):  
J. L. Hancock
Keyword(s):  
Sperm Concentration ◽  
Morphological Characteristics ◽  
Reducing Power ◽  
Cover Glass ◽  
Tubular Atrophy ◽  
Histological Features ◽  
Middle Piece ◽  
Semen Characteristics ◽  
Boar Semen ◽  
The Mean

Two procedures were followed to train boars to mount a dummy sow for semen collections. Of thirty-nine boars which were introduced to the dummy sow with no previous training twelve were trained successfully. Of eight boars which were first allowed daily mating with an oestrous sow in the pen housing the dummy sow, seven were trained successfully.Measurements of semen characteristics were made on twenty-five fertile boars including nine boars (morphological characteristics only) in use at commercial insemination centres.The mean ejaculate volume of thirty-four ‘first’ ejaculates was 173·7 ml. (range 85–275 ml.). The mean volume of the fluid fraction was 109·3 ml. (range 15·220 ml.). For second ejaculates these values were 156·9 ml. (range 80·220 ml.) and 93·2 ml. (range 25·165 ml.).The mean sperm concentration in thirty-five ‘first’ ejaculates was 284·5 x 106/ml. (range 11 × 106/ml. to 925 × 106/ml.). The mean concentration for ten second ejaculates was 224·4 × 106/ml. (range 18 × 106 to 295 × 106/ml.).In samples collected in successive 20 ml. fractions the highest concentration was found in the first 20–40 ml. In some boars the spermatozoa were distributed more uniformly throughout the ejaculate than in others.The mean number of spermatozoa per first ejaculate was 28·26 × 109 (range 0·77 × 109 to 80·0 × 109). The mean for second ejaculates was 15·88 × 109 (range 0·76 × 109 to 44·17 × 109). The mean initial pH of thirty-four first ejaculates was 7·22 (range 6·85–7·9); for second ejaculates it was 7·54 (range 7·22–8·0).There was a marked increase of pH with time after collection when semen was stored in open vessels; this did not occur when semen was stored in filled stoppered vessels.The mean pH of the sperm fraction in six ejaculates was 7·06 (range 6·8–7·4); the mean pH for post-sperm fractions of the same ejaculates was 7·36 (range 7·0–7·6).The freezing points of three ejaculates were –0·54, –0·55 and –0·56° C.The mean methylene blue reduction time for seven ejaculates was 5·0 min. (range 2·5–6·5 min.). The reducing power of boar semen was virtually unaffected when all the spermatozoa were killed by freezing.Motility of boar semen was lost rapidly when it was examined under a cover-glass; motility was restored by aeration (removal and replacement of the cover-glass).The mean percentages of nine morphological classes of spermatozoa in ejaculated semen of fertile boars were as follows: Malformed heads, 3·0; malformed middle-pieces, 2·7; bent tails, 4·5; coiled tails, 0·9; headless, 0·3; tailless, 0·3; broken necks, 0·1; neck beads, 11·8; middle-piece beads, 17·18.The decline in the concentration of spermatozoa in successive fractions of an ejaculate was associated with a decline in the frequency of middle-piece beads.The semen of one sterile boar showed a mean frquency of 77% malformed middle-pieces.The mean percentages of the above classes in samples from the vasa deferentia of nineteen fertile boars were: malformed heads, 5·7; malformed middle-pieces, 5·1; bent tails, 3·6; coiled tails, 0·06; headless, 0·6; tailless, 0·3; broken necks, 0·1; neck beads, 17·4; middle-piece beads, 59·2.The average percentages in samples from vasa deferentia of four sterile boars were: malformed heads, 11·9; malformed middle-pieces, 30·7; bent tails, 9·1; coiled tails, 0·5; headless, 0·4; tailless, 0·5; fractured necks, 0·9; neck beads, 24·2; middlepiece beads, 30·0.The mean testis and epididymis weights were 359·2 and 84·7 g. Expressed as percentages of body weight the weights were 0·285 and 0·068. The mean within pair differences in weights were 48·9 g. (between testes) and 8·5 g. (between epididymides).The macroscopic and microscopic histological features of the testes are described. Tubular atrophy associated with impaction was found to be a common feature of this sample of boar testes. There was no clear relationship of the histological features of the testes either to semen characteristics or to fertility. It is shown that fertile boars may show grossly pathological testes characteristics. The cause of the observed pathological changes was not identified. The findings are discussed.

Fresh and Post-Thaw Seminal Attributes of Jafarabadi Buffalo Bulls

2020 ◽  
Vol 15 (03) ◽  
pp. 45-48
Author(s):  
K. H. Parmar ◽  
FS Kavani ◽  
TK Patbandha ◽  
SS Parikh
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Conception Rate ◽  
Mean Values ◽  
Abnormal Sperm ◽  
The Mean ◽  
Live Sperm ◽  
One Year ◽  
Acrosomal Integrity

The study was conducted on four Jafarabadi breeding bulls, 5-6 years old to know the fresh and post-thawed seminal characteristics based on total of 192 semen ejaculates evaluated and cryopreserved over one year period. The mean values of fresh neat seminal characteristics of Jafarabadi bulls, viz., ejaculate volume (ml), colour/density (score), sperm concentration (million/ml), mass activity (score), initial motility (%), live sperm (%), abnormal sperm (%), HOS reactive sperm (%) and acrosomal integrity (%) were 5.19±0.18, 2.38±0.10, 1253.36±24.75, 3.73±0.05, 80.31±0.05, 86.20±0.64, 5.00±0.40, 85.75±0.43 and 93.56±0.56, respectively, whereas the mean post-thawed sperm characteristics, viz., progressive sperm motility, live sperm (%), abnormal sperm (%), HOS reactive sperm (%), acrosomal integrity (%) and first insemination conception rate (%) observed were 57.60±0.36, 66.34±0.53, 8.85±0.33, 56.97±0.46, 75.26±0.17 and 44.63±0.14, respectively. The semen quality of fresh and post-thawed samples observed was within normal limit for use in breeding program with satisfactory first insemination conception rate.

Utilization of microsatellite markers in genotyping of Saudi Arabian camels for productivity and conservation

2020 ◽  
Vol 100 (2) ◽  
pp. 253-261
Author(s):  
Ahmed Hossam Mahmoud ◽  
Mohammad Abul Farah ◽  
Ahmed Rady ◽  
Khalid Mashai Alanazi ◽  
Osama Mohammed ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Genetic Distances ◽  
Kingdom Of Saudi Arabia ◽  
Breeding Programs ◽  
Expected Heterozygosity ◽  
Exotic Breed ◽  
Overdominant Selection ◽  
Ssr Loci ◽  
The Mean ◽  
Simple Sequence

Camels are considered as integral and notable components of the heritage of the Kingdom of Saudi Arabia. Genetic variabilities within and among four camel populations in Saudi Arabia were evaluated using 21 simple sequence repeat (SSR) loci of 122 unrelated individuals, including three indigenous breeds [Humur (HA), Zurg (ZR), Shuguh (SG)] and one exotic breed [Sudanese (SN)]. Nineteen SSR markers generated multilocus fingerprints with a total of 225 alleles, a range of 4–23 alleles per locus, and an average of 9, 7, 7, and 6 alleles per locus in HA, ZR, SG, and SN populations, respectively. The mean multilocus FST value (0.034 ± 0.005) showed non-significant population differentiation. Mean observed heterozygosity values were 0.908 for HA, 0.860 for ZR, 0.919 for SG, and 0.887 for SN, which were higher than the expected heterozygosity. An excess of heterozygotes was observed, suggesting the presence of overdominant selection or the occurrence of outbreeding. Pairwise genetic distances indicated that the three indigenous camel breeds were genetically close to each other and genetically distant to the SN population. This genetic variability assessment by microsatellite analysis is important and useful for the conservation of local camel genetic resources as well as the future development of breeding programs.

scholarly journals Seminal Attributes, Freezability and their Interrelationships in Zebu Cattle and Buffalo Bulls from Central Gujarat

2018 ◽  
Vol 14 (2) ◽  
Author(s):  
P. K. Pathak ◽  
A. J. Dhami ◽  
D. V. Chaudhari
Keyword(s):  
Semen Quality ◽  
Conventional Technique ◽  
Zebu Cattle ◽  
Motile Sperm ◽  
Morphological Attributes ◽  
Frozen Semen ◽  
The Mean ◽  
Live Sperm ◽  
Sperm Output ◽  
Fresh Semen

A study was carried out on nine healthy mature breeding bulls (3 each of Gir, Surti and Murrah breed) to evaluate their fresh and frozen semen quality and their interrelationships. The ejaculates immediately after collection were evaluated for routine physico- morphological attributes, including HOS test. The ejaculates (n=72) having >75% initial motility were diluted @ 80 million sperm/ml using TFYG extender and the French mini straws filled were frozen in liquid nitrogen vapour using a programmable biofreezer. Thawing of straws was done at 37°C for 30 sec and assessed for freezability by conventional technique. All the cattle and buffalo bulls donated consistently normal thick creamy yellow and thick milky white semen, respectively. In Gir, Suti and Murrah bulls (n=24 ejaculate each) the seminal attributes such as ejaculate volume (6.69±0.17, 3.12±0.10 and 3.96±0.16 ml, p less than 0.01); initial motility (80.21±0.88, 84.58±0.60 and 84.38±0.76 %, p less than 0.01); total sperm output/ejaculate (9013.85±265.32, 3935.49±259.63 and 5366.48±332.99 million, p less than 0.01) and live sperm (84.71±0.83, 86.17±0.78 and 86.79±0.79 %, p less than 0.05) differed significantly. The mean percentages of post-thaw motile sperm (53.29±1.56, 58.33±1.43 and 59.58±1.20, p less than 0.01); live sperm (59.00±1.95, 67.00±1.59 and 68.42±1.66 %, p less than 0.01); and HOS reactive sperm (48.25±0.78, 44.21±1.29 and 51.54±1.29 %, p less than 0.01) in Gir, Surti and Murrah bulls semen also differed significantly. The variation among the bulls was significant for buffalo breeds in most of their fresh seminal attributes, except HOST, and for post-thaw motility, but not among Gir bulls. The important seminal attributes like motility, live sperm and HOS reactive sperm of fresh and frozen-thawed semen were significantly and positively interrelated in all three breeds of bulls (r = 0.40 to 0.81, p less than 0.05 to 0.01), suggesting that motility and HOST of fresh semen were good predictors of freezability of bovine semen.

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