Effect of arsenic on growth, lipid peroxidation and antioxidant defence system in cowpea

2019 ◽  
Author(s):  
Faheema Khan
Keyword(s):  
Lipid Peroxidation ◽  
Plant Root ◽  
Enzymatic Antioxidants ◽  
Antioxidant Defence ◽  
Defence System ◽  
Hydroponic Experiment ◽  
Root And Shoot Length ◽  
Antioxidant Defence System ◽  
Mda Content ◽  
As Stress

Arsenic (As) is a toxic ubiquitous metalloid. Exposure of plants to As can result in various morphological, physiological and biochemical variations. Hydroponic experiment was conducted to study the growth response, lipid peroxidation, proline and antioxidant defence system under arsenic (As) stress in the cowpea. Ten day old seedlings of cowpea grown hydroponically, were treated with 0, 25, 50, 75 and 100 µM (Na2HAsO4) sodium arsenate for 7 days and analysed for morphological and biochemical traits under As stress. Significant decline in plant root and shoot length along with biomass was recorded with increased arsenic doses, as compared to control. The As treatment resulted in increased proline and decrease in malondialdehyde (MDA) content in seedlings of cowpea with increased concentration. Enzymatic antioxidants like catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR) showed increased activity in dose dependant manner over control under As stress. Results indicated lower content of MDA and enhanced activities of the enzymatic antioxidant perform a significant role in As tolerance in cowpea.

scholarly journals Effects of dietary supplementation with red-pigmented leafy lettuce (Lactuca sativa) on lipid profiles and antioxidant status in C57BL/6J mice fed a high-fat high-cholesterol diet

2009 ◽  
Vol 101 (8) ◽  
pp. 1246-1254 ◽  
Author(s):  
Jeung Hee Lee ◽  
Penelope Felipe ◽  
Yoon Hyung Yang ◽  
Mi Yeon Kim ◽  
Oh Yoon Kwon ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Lactuca Sativa ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
High Fat ◽  
Antioxidant Defence ◽  
Defence System ◽  
Freeze Dried ◽  
Antioxidant Defence System

The present study was undertaken to assess the beneficial effects of a daily consumption of 8 % freeze-dried red-pigmented leafy lettuce (Lactuca sativa) on CVD. C57BL/6J mice were fed a high-fat high-cholesterol diet supplemented with or without red-pigmented leafy lettuce for 4 weeks. The present results showed that the red-pigmented leafy lettuce-supplemented diet significantly decreased the level of total and LDL-cholesterol and TAG in the plasma of the mice. The atherosclerotic index was calculated to be 46 % lower in the mice fed with the lettuce diet compared with the control diet. Lipid peroxidation measured by 2-thiobarbituric acid-reactive substances was markedly reduced in the plasma, liver, heart and kidney of the mice fed the lettuce diet. The content of antioxidants (total glutathione and β-carotene) was significantly increased by lettuce supplementation. The antioxidant defence system by antioxidant enzymes including glutathione S-transferase, glutathione peroxidase, glutathione reductase, superoxide dismutase and paraoxanase in blood or liver tissues was also increased, and showed the improved oxidative stress in the mice fed the lettuce diet. The measurement of tail DNA (%), tail extent moment and olive tail moment indicated that the lettuce diet increased the resistance of hepatocyte and lymphocyte DNA to oxidative damage. The present study showed that the supplementation of a high-cholesterol high-fat diet with 8 % red-pigmented leafy lettuce resulted in an improvement of plasma cholesterol and lipid levels, prevention of lipid peroxidation and an increase of the antioxidant defence system and, therefore, could contribute to reduce the risk factors of CVD.

scholarly journals Copper sulphate impact on the antioxidant defence system of the marine bivalves Cerastoderma edule and Scrobicularia plana

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Andreia F. Mesquita ◽  
Sérgio M. Marques ◽  
João C. Marques ◽  
Fernando J. M. Gonçalves ◽  
Ana M. M. Gonçalves
Keyword(s):  
Lipid Peroxidation ◽  
Copper Sulphate ◽  
Aquatic Systems ◽  
Marine Bivalve ◽  
Antioxidant Defence ◽  
Cerastoderma Edule ◽  
Size Classes ◽  
Defence System ◽  
Scrobicularia Plana ◽  
Antioxidant Defence System

Abstract Anthropogenic activities, such as agriculture and industrial activities, are a main source of pollution contributing for the degradation of water quality and thus affecting the living organisms of aquatic systems. Copper is widely used at these practices being often released into the aquatic systems and may cause negative effects in its communities. This study proposes to determine the effects of copper in the antioxidant defence system of two size classes (big and small sizes) of Scrobicularia plana and Cerastoderma edule, two marine bivalve species with commercial interest. It was observed the behaviour activity of the organisms during the exposure to copper sulphate (CS) and was determined the enzymatic activities of glutathione-S-transferases (GST), glutathione reductase (GR) and glutathione peroxidase (GPx) (both selenium-dependent (SeGPx) and total (tGPx)) in the muscle tissue (foot). Lipid peroxidation (LPO) was evaluated through thiobarbituric acid reactive substances (TBARS) measurement in the foot. Changes in the behaviour and enzymatic activity were observed. Lipid peroxidation was observed at C. edule and S. plana big and small size classes, respectively, according to TBARS levels. The foot showed to be a good tissue to be used in biochemical analysis to detect the presence of toxicants.

scholarly journals UV-vis spectroscopic study of lipid peroxidation and antioxidant contents of kidney of Albino mice co-administrated with sildenafil citrate and alcohol

2011 ◽  
Vol 26 (4-5) ◽  
pp. 279-288
Author(s):  
K. Soumya ◽  
Raju Udayakumar ◽  
K. Viswanathan
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Free Radicals ◽  
Spectroscopic Study ◽  
Sildenafil Citrate ◽  
Antioxidant Defence ◽  
Defence System ◽  
Antioxidant Defence System ◽  
Albino Mice ◽  
Enzymic Antioxidants

Male Wistar Albino mice were co-administrated with appropriate dose of sildenafil citrate and alcohol. The kidney samples of those animals were analyzed for lipid peroxidation and antioxidant contents. Lipid peroxidation was measured based on TBARS. Antioxidant such as SOD, CAT, GPx and GSH were also determined. From the present investigation, an enhanced level of TBARS accompanied by a decreased concentration of enzymic as well as non-enzymic antioxidants in kidney was detected for 30 days animals treated concomitantly with sildenafil citrate and alcohol. Increased level of lipid peroxidation lead to accumulation of free radicals and increased oxidative threat in kidney tissues. Also, the decreased concentration of antioxidant enzymes in renal tissues indicates the failure of antioxidant defence system.

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