scholarly journals UV-vis spectroscopic study of lipid peroxidation and antioxidant contents of kidney of Albino mice co-administrated with sildenafil citrate and alcohol

2011 ◽  
Vol 26 (4-5) ◽  
pp. 279-288
Author(s):  
K. Soumya ◽  
Raju Udayakumar ◽  
K. Viswanathan
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Free Radicals ◽  
Spectroscopic Study ◽  
Sildenafil Citrate ◽  
Antioxidant Defence ◽  
Defence System ◽  
Antioxidant Defence System ◽  
Albino Mice ◽  
Enzymic Antioxidants

Male Wistar Albino mice were co-administrated with appropriate dose of sildenafil citrate and alcohol. The kidney samples of those animals were analyzed for lipid peroxidation and antioxidant contents. Lipid peroxidation was measured based on TBARS. Antioxidant such as SOD, CAT, GPx and GSH were also determined. From the present investigation, an enhanced level of TBARS accompanied by a decreased concentration of enzymic as well as non-enzymic antioxidants in kidney was detected for 30 days animals treated concomitantly with sildenafil citrate and alcohol. Increased level of lipid peroxidation lead to accumulation of free radicals and increased oxidative threat in kidney tissues. Also, the decreased concentration of antioxidant enzymes in renal tissues indicates the failure of antioxidant defence system.

Queuine promotes antioxidant defence system by activating cellular antioxidant enzyme activities in cancer

2008 ◽  
Vol 28 (2) ◽  
pp. 73-81 ◽  
Author(s):  
Chandramani Pathak ◽  
Yogesh K. Jaiswal ◽  
Manjula Vinayak
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Mouse Liver ◽  
Similar Rate ◽  
Antioxidant Enzyme Activities ◽  
Antioxidant Defence ◽  
Defence System ◽  
Antioxidant Defence System

Constant generation of ROS (reactive oxygen species) during normal cellular metabolism of an organism is generally balanced by a similar rate of consumption by antioxidants. Imbalance between ROS production and antioxidant defence results in an increased level of ROS, causing oxidative stress, which leads to promotion of malignancy. Queuine is a hyper-modified base analogue of guanine, found at the first anticodon position of the Q-family of tRNAs. These tRNAs are completely modified with respect to queuosine in terminally differentiated somatic cells; however, hypo-modification of Q-tRNAs is closely associated with cell proliferation. Q-tRNA modification is essential for normal development, differentiation and cellular function. Queuine is a nutrient factor for eukaryotes. It is found to promote the cellular antioxidant defence system and inhibit tumorigenesis. The activities of antioxidant enzymes such as catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase are found to be low in the DLAT (Dalton's lymphoma ascites transplanted) mouse liver compared with normal mouse liver. However, exogenous administration of queuine to the DLAT cancerous mouse improves the activities of antioxidant enzymes. These results suggest that queuine promotes the antioxidant defence system by increasing antioxidant enzyme activities and in turn inhibits oxidative stress and tumorigenesis.

scholarly journals Change in lipid peroxidation intensity and activity of enzymes of antioxidant defence system in tissue of the bird’s kidney under effect of sodium hypochlorite of different concentration

2011 ◽  
Vol 5 (1) ◽  
pp. 77-84
Author(s):  
N. P. Holovchak ◽  
◽  
H. I. Kotsyumbas ◽  
M. B. Galan ◽  
M. Y. Boyko ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Sodium Hypochlorite ◽  
Antioxidant Defence ◽  
Defence System ◽  
Antioxidant Defence System ◽  
Activity Of Enzymes

Temporal variation in the antioxidant defence system and lipid peroxidation in the gills and mantle of hydrothermal vent mussel Bathymodiolus azoricus

2006 ◽  
Vol 53 (7) ◽  
pp. 1101-1116 ◽  
Author(s):  
Rui Company ◽  
Angela Serafim ◽  
Richard Cosson ◽  
Aline Fiala-Médioni ◽  
David Dixon ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Temporal Variation ◽  
Hydrothermal Vent ◽  
Antioxidant Defence ◽  
Defence System ◽  
Antioxidant Defence System

scholarly journals Lipid Peroxidation and Antioxidant Defence System in Patients with Active or Inactive Behçet's Disease

2003 ◽  
Vol 83 (5) ◽  
pp. 342-346 ◽  
Author(s):  
R. Sandikci ◽  
S. Türkmen ◽  
G. Güvenen ◽  
H. Ayabakan ◽  
P. Gülcan ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Behçet’S Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Antioxidant Defence ◽  
Behcet's Disease ◽  
Defence System ◽  
Antioxidant Defence System

scholarly journals Effects of dietary supplementation with red-pigmented leafy lettuce (Lactuca sativa) on lipid profiles and antioxidant status in C57BL/6J mice fed a high-fat high-cholesterol diet

2009 ◽  
Vol 101 (8) ◽  
pp. 1246-1254 ◽  
Author(s):  
Jeung Hee Lee ◽  
Penelope Felipe ◽  
Yoon Hyung Yang ◽  
Mi Yeon Kim ◽  
Oh Yoon Kwon ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Lactuca Sativa ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
High Fat ◽  
Antioxidant Defence ◽  
Defence System ◽  
Freeze Dried ◽  
Antioxidant Defence System

The present study was undertaken to assess the beneficial effects of a daily consumption of 8 % freeze-dried red-pigmented leafy lettuce (Lactuca sativa) on CVD. C57BL/6J mice were fed a high-fat high-cholesterol diet supplemented with or without red-pigmented leafy lettuce for 4 weeks. The present results showed that the red-pigmented leafy lettuce-supplemented diet significantly decreased the level of total and LDL-cholesterol and TAG in the plasma of the mice. The atherosclerotic index was calculated to be 46 % lower in the mice fed with the lettuce diet compared with the control diet. Lipid peroxidation measured by 2-thiobarbituric acid-reactive substances was markedly reduced in the plasma, liver, heart and kidney of the mice fed the lettuce diet. The content of antioxidants (total glutathione and β-carotene) was significantly increased by lettuce supplementation. The antioxidant defence system by antioxidant enzymes including glutathione S-transferase, glutathione peroxidase, glutathione reductase, superoxide dismutase and paraoxanase in blood or liver tissues was also increased, and showed the improved oxidative stress in the mice fed the lettuce diet. The measurement of tail DNA (%), tail extent moment and olive tail moment indicated that the lettuce diet increased the resistance of hepatocyte and lymphocyte DNA to oxidative damage. The present study showed that the supplementation of a high-cholesterol high-fat diet with 8 % red-pigmented leafy lettuce resulted in an improvement of plasma cholesterol and lipid levels, prevention of lipid peroxidation and an increase of the antioxidant defence system and, therefore, could contribute to reduce the risk factors of CVD.

scholarly journals Copper sulphate impact on the antioxidant defence system of the marine bivalves Cerastoderma edule and Scrobicularia plana

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Andreia F. Mesquita ◽  
Sérgio M. Marques ◽  
João C. Marques ◽  
Fernando J. M. Gonçalves ◽  
Ana M. M. Gonçalves
Keyword(s):  
Lipid Peroxidation ◽  
Copper Sulphate ◽  
Aquatic Systems ◽  
Marine Bivalve ◽  
Antioxidant Defence ◽  
Cerastoderma Edule ◽  
Size Classes ◽  
Defence System ◽  
Scrobicularia Plana ◽  
Antioxidant Defence System

Abstract Anthropogenic activities, such as agriculture and industrial activities, are a main source of pollution contributing for the degradation of water quality and thus affecting the living organisms of aquatic systems. Copper is widely used at these practices being often released into the aquatic systems and may cause negative effects in its communities. This study proposes to determine the effects of copper in the antioxidant defence system of two size classes (big and small sizes) of Scrobicularia plana and Cerastoderma edule, two marine bivalve species with commercial interest. It was observed the behaviour activity of the organisms during the exposure to copper sulphate (CS) and was determined the enzymatic activities of glutathione-S-transferases (GST), glutathione reductase (GR) and glutathione peroxidase (GPx) (both selenium-dependent (SeGPx) and total (tGPx)) in the muscle tissue (foot). Lipid peroxidation (LPO) was evaluated through thiobarbituric acid reactive substances (TBARS) measurement in the foot. Changes in the behaviour and enzymatic activity were observed. Lipid peroxidation was observed at C. edule and S. plana big and small size classes, respectively, according to TBARS levels. The foot showed to be a good tissue to be used in biochemical analysis to detect the presence of toxicants.

Anti-carcinogenic action of curcumin by activation of antioxidant defence system and inhibition of NF-κB signalling in lymphoma-bearing mice

2011 ◽  
Vol 32 (2) ◽  
pp. 161-170 ◽  
Author(s):  
Laxmidhar Das ◽  
Manjula Vinayak
Keyword(s):  
Oxidative Stress ◽  
Cell Proliferation ◽  
Antioxidant Enzymes ◽  
Morphological Changes ◽  
Nuclear Factor Κb ◽  
Antioxidant Defence ◽  
Defence System ◽  
Antioxidant Defence System ◽  
Endogenous Antioxidant ◽  
Endogenous Antioxidant Defence

NF-κB (nuclear factor κB) plays a significant role in inflammation, immunity, cell proliferation, apoptosis and malignancy. ROS (reactive oxygen species) are among the most important regulating factors of NF-κB. Intracellular ROS are mainly regulated by an endogenous antioxidant defence system. Any disruption of redox balance leads to oxidative stress, which causes a number of pathological conditions including inflammation and malignancy. Increased metabolic activity in cancerous cells leads to oxidative stress, which is further enhanced due to depletion of the endogenous antioxidant defence system. However, the activation and signalling of NF-κB are reported to be inhibited by overexpression and induced activity of antioxidant enzymes. Therefore the present study focuses on the correlation between the endogenous antioxidant defence system, ROS and NF-κB activation during lymphoma growth in mice. The study highlights the anti-carcinogenic role of curcumin by modulation of NF-κB activation and oxidative stress via the endogenous antioxidant defence system. Oxidative stress was monitored by lipid peroxidation, protein carbonylation and antioxidant enzyme activity. NF-κB-mediated signalling was tested by DNA-binding activity. The results reflect that intracellular production of H2O2 in oxidative tumour micro-environment regulates NF-κB activation. Curcumin inhibits oxidative state in the liver of lymphoma-bearing mice by enhancing the transcription and activities of antioxidant enzymes, which in turn modulate activation of NF-κB, leading to a decrease in lymphoma growth. Morphological changes as well as cell proliferation and cell survival assays confirmed reduced lymphoma growth. Thus curcumin contributes to cancer prevention by disrupting the vicious cycle of constant ROS production, responsible for a high oxidative micro-environment for tumour growth.

Markers of antioxidant defence system and lipid peroxidation in peripheral blood of female patients with chronic idiopathic urticaria

2006 ◽  
Vol 298 (10) ◽  
pp. 499-503 ◽  
Author(s):  
Alicja Kasperska-Zajac ◽  
Zenon Brzoza ◽  
Renata Polaniak ◽  
Barbara Rogala ◽  
Ewa Birkner
Keyword(s):  
Lipid Peroxidation ◽  
Peripheral Blood ◽  
Chronic Idiopathic Urticaria ◽  
Antioxidant Defence ◽  
Female Patients ◽  
Defence System ◽  
Antioxidant Defence System
Sign in / Sign up

Export Citation Format

Share Document