scholarly journals Variasi Diameter Kolom dan Rasio Sampel-Silika pada Isolasi Steroid dan Triterpenoid Alga Merah Eucheuma cottonii dengan Kromatografi Kolom Basah

ALCHEMY ◽  
2018 ◽  
Vol 6 (2) ◽  
pp. 57
Author(s):  
Ahmad Ghanaim Fasya ◽  
Ariska Purwaning Tyas ◽  
Fitroh Annasaul Mubarokah ◽  
Rachmawati NIngsih ◽  
Armeida D. R. Madjid
Keyword(s):  
Thin Layer ◽  
Column Chromatography ◽  
Active Compound ◽  
Red Algae ◽  
Functional Group ◽  
Ftir Analysis ◽  
Column Diameter ◽  
Silica Ratio ◽  
Layer Chromatography ◽  
Eucheuma Cottonii

<p>Optimizing steroid and triterpenes isolation from red algae <em>Eucheuma cottonii</em> was done using column chromatography by varying of column diameter (1, 1.5 and 2 cm) and a ratio of sample : silica (1:50, 1:100, and 1:150). Active compound of <em>E. cottonii</em> was extracted by maceration with methanol. Then, the extract was hydrolyzed using HCl 2 N and partitioned with petroleum ether. The extract after partitioned was separated using wet column chromatography. The result of separation was monitored using analytical thin layer chromatography (Analytical TLC) and identified using FTIR. The best separation was aimed using diameter column 1 cm and a ratio of sample : silica 1:150. Based on FTIR analysis, the steroid from extract had a functional group –OH, –C–H, C=O, -C(CH<sub>3</sub>)<sub>2</sub>, and C-OH alcohol and the triterpenoid from extract had a functional group –OH, C–H, C=O, C=C, -C(CH<sub>3</sub>)<sub>2</sub> and C-OH alcohol.</p><p>Keywords: <em>E. cottonii</em>, column chromatography, steroid, triterpenoid, column size, sample : gel silica ratio</p><p> </p><p> </p><p><span>Optimasi isolasi senyawa steroid dan triterpenoid dalam alga merah <em>Euchema cottonii</em> telah dilakukan menggunakan kromatografi kolom dengan variasi diameter kolom (1; 1,5; dan 2 cm) dan rasio sampel : silika (1:50; 1:100; dan 1:150). Ekstraksi senyawa aktif pada <em>E. cottonii</em> dilakukan dengan maserasi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis menggunakan HCl 2 N dan dipartisi dengan petroleum eter. Hasil pemisahan dimonitoring menggunakan KLTA dan diidentifikasi menggunakan FTIR. Hasil penelitian menunjukkan pemisahan kromatografi kolom terbaik diperoleh dengan variasi diameter 1 cm dan variasi rasion sampel : silika 1:150. Hasil analisis senyawa steroid memberikan informasi gugus –OH, –C–H, C=O, -C(CH<sub>3</sub>)<sub>2</sub>, dan C-OH alkohol, sedangkan senyawa triterpenoid memberikan informasi gugus –OH, –C–H, C=O, C=C, -C(CH<sub>3</sub>)<sub>2</sub> dan C-OH alkohol.</span></p><p><span><span>Kata Kunci: <em>E. cottonii</em>, kromatografi kolom, steroid, triterpenoid, ukuran kolom, rasio sampel : silika gel</span></span></p>

scholarly journals UJI TOKSISITAS DAN IDENTIFIKASI SENYAWA EKSTRAK ALGA MERAH (Eucheuma cottonii) TERHADAP LARVA UDANG Artemia salina LEACH

ALCHEMY ◽  
2013 ◽  
Author(s):  
Noer Mardlatillah Sharo ◽  
Rachmawati Ningsih ◽  
Ahmad Hanapi ◽  
Ahmad Nasichuddin
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Red Algae ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Active Compounds ◽  
Layer Chromatography ◽  
Eucheuma Cottonii

<p><em>Eucheuma cottonii</em> is a multicellular algae (multicellular) that have bioactive compounds used in medicine, such as anticancer. The purpose of this research is to know the level of toxicity of the crude extract of ethanol and n-hexane red algae (<em>Eucheuma cottonii</em>) against larvae of shrimp <em>A</em><em>. salina</em> Leach and to know the active compounds contained in crude extracts of ethanol and n-hexane red algae (<em>Eucheuma cottonii</em>).</p><p>This research was conducted with the maceration extraction continuing by test toxicity against the shrimp larvae of <em>A</em><em>. salina</em> Leach (BSLT). The result of the toxicity test then proceeds with the test reagents and phytochemicals with thin layer chromatography for positive test of compound phytochemicals. Thin-layer chromatography using eluen n-hexane: ethyl acetate (7: 3) to ethanol extract and n-hexane: ethyl acetate (17: 3) for n-hexane extract. Data on toxicity of <em>A. salina Leach</em><em> </em>analyzed by probit analysis to find out the value of LC<sub>50</sub> on each extract.</p><p>The results of this research indicate that each of the rough red algae extract (<em>Eucheuma cottonii</em>) by using a solvent of ethanol and n-hexane have toxicity values (LC<sub>50</sub>) of shrimp larvae of <em>A</em><em>. salina</em> Leach consecutive 58,0128 ppm and 61,7571 ppm. Active compounds content of Phytochemical test results i.e. triterpenoid (ethanol extract) and steroids (n-hexsane extract). The TLC triterpenoid obtained 7 stains, and the results obtained steroids 8 TLC stains.</p>

scholarly journals Analisis Senyawa Toksik Tepung Pelepah Batang Aren (Arenga pinnata) dengan Spektroskopi UV-Vis dan Inframerah

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (2) ◽  
pp. 54
Author(s):  
Irma Dukomalamo ◽  
Meiske Sientje Sangi ◽  
Johnly Alfreds Rorong
Keyword(s):  
Infrared Spectroscopy ◽  
Thin Layer ◽  
Column Chromatography ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Functional Group ◽  
Artemia Salina ◽  
Toxic Compounds ◽  
Brine Shrimp Lethality ◽  
Layer Chromatography

Telah dilakukan penelitian mengenai analisis senyawa toksik tepung pelepah batang aren (A. pinnata) dengan spektroskopi UV-Vis dan inframerah dengan tujuan untuk mengisolasi senyawa toksik dari tepung pelepah batang aren dan menganalisis senyawa toksik tersebut dengan spektroskopi UV-Vis dan inframerah. Tepung pelepah batang aren diekstraksi dengan cara maserasi menggunakan pelarut etanol hasil redestilasi cap tikus. Ekstrak diuji dengan Kromatografi Lapis Tipis (KLT) untuk menentukan jenis eluen terbaik untuk dipisahkan dengan kromatografi kolom sehingga diperoleh fraksi-fraksi. Fraksi hasil kromatografi kolom diuji KLT ulang, noda sama dari beberapa fraksi digabungkan. Fraksi yang hanya memiliki 1 noda dilakukan uji toksisitas dengan metode Brine Shrimp Lethality Test (BSLT) menggunakan larva udang Artemia salina L. dan dianalisis menggunakan spektroskopi UV-Vis dan inframerah. Hasil pengujian KLT diperoleh 6 noda dengan nilai Rf yaitu noda (a) 0,961; (b) 0,857; (c) 0,779; (d) 0,714; (e) 0,623; (f) 0,376. Fraksi A hasil kromatografi kolom dengan noda tunggal yang dilakukan uji KLT Rf = 0,961. Fraksi A yang diuji toksisitas menunjukkan fraksi bersifat toksik dengan nilai LC50 sebesar 269,15 ppm. Analisis spektrofotometer UV-Vis dan Inframerah menunjukkan bahwa fraksi memiliki gugus fungsi –C=O, -C-O ester, -C=C alkena, serta C-C dan C-H alkana. Dari hasil penelitian dapat disimpulkan bahwa senyawa yang diisolasi dari tepung pelepah batang aren bersifat toksik dengan nilai LC50 sebesar 269,15 ppm dan merupakan senyawa nonpolar yang memiliki gugus fungsi –C=O, -C-O ester, -C=C alkena, serta C-C dan C-H alkana.A research has been done about Analysis of Toxic Compounds Sugar Palm Stem Midrib Flour (A. Pinnata) using UV-Vis and Infrared Spectroscopy, aimed to isolated of toxic compounds sugar palm stem midrib flour and analyze using UV-Vis and infrared spectroscopy. Sugar palm stem midrib flour extracted by maceration using ethanol as solvent results from redistillation cap tikus. The extract was tested by Thin Layer Chromatography (TLC) to determine the best eluent for separated with column chromatography to obtained fractions. Fractions column chromatography results were tested using KLT, the same spot of fractions were combined. Fraction having 1 spot was toxicity test performed by the method of Brine Shrimp Lethality Test (BSLT) using Artemia salina L. larvae and analyze using UV-Vis and infrared spectroscopy. Results KLT testing obtained six spots with Rf is  (a) 0,961; (b) 0,857; (c) 0,779; (d) 0,714; (e) 0,623; (f) 0,376. Fraction A column chromatography results having one spot by KLT test with Rf = 0,961. The results of toxicity test showed fraction A was toxic with LC50 269,15 ppm. Analysis spectrophotometer UV-Vis and inframerah showed that fraction had functional group like –C=O, -C-O esther, -C=C alkene, C-C and C-H alkane. This research concludes compound of sugar palm stem midrib flour isolated sugar palm stem midrib flour and nonpolar compound with functional group like –C=O, -C-O esther, -C=C alkene, C-C and C-H alkane.

Notizen: Isolation of Batatasin I from Dioscorea dumetorum Rhizomes

1979 ◽  
Vol 34 (3-4) ◽  
pp. 288-289 ◽  
Author(s):  
M. M. El-Olemy ◽  
J. Reisch
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Spectral Evidence ◽  
Layer Chromatography

Abstract Batatasin I was shown to occur in the fresh rhizomes of poisonous yam (Dioscorea dumetorum Pax.,). It was isolated by column chromatography and identified by chromatographic and spectral evidence. Two other unknown phenanthrenes were shown to occur by thin-layer chromatography.

Comparison of Different Soil Leaching Techniques with Four Herbicides

Weed Science ◽  
1975 ◽  
Vol 23 (6) ◽  
pp. 508-511 ◽  
Author(s):  
Chu-Huang Wu ◽  
P. W. Santelmann
Keyword(s):  
Thin Layer ◽  
Column Chromatography ◽  
Waiting Times ◽  
Thick Layer ◽  
Working Hours ◽  
Laboratory Methods ◽  
Thin Layer Method ◽  
Rf Values ◽  
Layer Chromatography ◽  
Herbicide Mobility

Herbicide mobility in soils was compared by three laboratory methods. The Rf values calculated from soil thin-layer chromatography correlated closely with those obtained from soil thick-layer chromatography (r = 0.96). Herbicides leached slightly further in slotted column chromatography as compared with the other methods. The working hours required to conduct a study with each method were in the increasing order of thin-layer, thick-layer, and column chromatography. However, the thin-layer method required the longest waiting times, followed by the column and thick-layer chromatography. If radioactive herbicides are not available or obtainable, the thick-layer chromatography is simplest and quickest. The relative mobility of herbicides studied was fluometuron [1,1-dimethyl-3-(α,α,α-trifluoro-m-tolyl)urea] > napropamide [2-(α-naphthoxy)-N,N-diethylpropionamide] > terbutryn [2-(tert-butyl-amino)-4-(ethylamino)-6-(methylthio)-s-triazine] > trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine). Less herbicide mobility was observed in heavier soil than in sandy soil.

Identification of Antioxidants, Accelerators and Plasticizers in Rubber Compounds by Column Chromatography and Ultraviolet and Infrared Spectrophotometry

1968 ◽  
Vol 41 (3) ◽  
pp. 630-637 ◽  
Author(s):  
A. Fiorenza ◽  
G. Bonomi ◽  
A. Saredi
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Column Chromatography ◽  
Infrared Spectrophotometry ◽  
Ultraviolet Detector ◽  
Rubber Compounds ◽  
Ir Spectrophotometry ◽  
Suitable Reference ◽  
Layer Chromatography ◽  
Reference Samples

Abstract This paper deals with separation of antioxidants, accelerators, and plasticizers in rubber compounds by column chromatography. The chromatographic diagrams obtained by a special ultraviolet detector and the spectra of the various fractions obtained from the extracts of eleven compounds are reported. With suitable reference samples, many other antioxidants and some other accelerators can be identified in addition to those tested in the present work. The identification of the components of the extract is improved examining the fractions also by IR spectrophotometry, employing either the technique of NaCl plates or, better, that of KBr pellets. Other very useful auxiliary techniques are paper and thin layer chromatography and gas chromatography.

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