scholarly journals Laporan Kasus: Dermatofitosis oleh Microsporum spp., dan Curvularia spp., pada Anjing Pomeranian

2021 ◽  
Vol 10 (5) ◽  
pp. 804-813
Author(s):  
Ni Made Ayu Kurniawati ◽  
I Gusti Made Krisna Erawan ◽  
I Gede Soma
Keyword(s):  
Skin Test ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Canis

Dermatofitosis merupakan infeksi jamur yang sering disebabkan oleh Microsporum canis, M. gypseum, dan Trichophyton mentagrophytes. Anjing dalam kasus ini merupakan anjing ras pomeranian jantan berusia lima tahun dengan bobot badan 6 kg. Anjing kasus menderita lesi kemerahan pada permukaan kulit, yang kemudian menjadi keropeng dan menyebar ke beberapa bagian tubuh secara multifokal. Anjing kasus sempat dibawa ke dokter hewan untuk pengobatan, namun lesi semakin parah dan obat sebelumnya tidak dapat dinilai. Pemeriksaan penunjang berupa tape skin test dilakukan untuk melihat agen penyebab penyakit. Sampel diambil dari lesi yang ada dipermukaan kulit, dan rambut hewan. Kapang Microsporum spp. dan Curvularia spp. ditemukan pada pemeriksaan tape skin test. Anjing diberikan pengobatan antijamur berupa itraconazole (5 mg/kg, q 24 jam) selama 28 hari dan cephalexin (15 mg/kg, q 12 jam) selama tujuh hari. Pengamatan pascaterapi menunjukkan keadaan hewan kasus kembali normal dengan pertumbuhan rambut yang bagus.

scholarly journals Expression of Ubiquitin Gene in Microsporum canis and Trichophyton mentagrophytes Cultured with Fluconazole

2001 ◽  
Vol 45 (9) ◽  
pp. 2559-2562 ◽  
Author(s):  
Rui Kano ◽  
Ken Okabayashi ◽  
Yuka Nakamura ◽  
Shinichi Watanabe ◽  
Atsuhiko Hasegawa
Keyword(s):  
Amino Acid ◽  
Antifungal Activity ◽  
Proteasome Inhibitor ◽  
Trichophyton Mentagrophytes ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Antifungal Drug ◽  
Microsporum Canis

ABSTRACT The expression of the ubiquitin (Ub) gene in dermatophytes was examined for its relation to resistance against the antifungal drug fluconazole. The nucleotide sequences and the deduced amino acid sequences of the Ub gene in Microsporum canis were proven to be 99% similar to those of the Ub gene in Trichophyton mentagrophytes. Expression of mRNA of Ub in M. canisand T. mentagrophytes was enhanced when the fungi were cultured with fluconazole. The antifungal activity of fluconazole against these dermatophytes was increased in the presence of Ub proteasome inhibitor.

scholarly journals Monitoring of Dermatophytosis incidence in domestic dogs and cats in Kharkiv, Ukraine

2019 ◽  
Vol 5 (1) ◽  
pp. 17-19
Author(s):  
G. V. Ponomarenko ◽  
V. L. Kovalenko ◽  
O. V. Ponomarenko ◽  
R. V. Severyn ◽  
A. M. Gontar ◽  
...  
Keyword(s):  
Laboratory Tests ◽  
Trichophyton Mentagrophytes ◽  
Domestic Dogs ◽  
Microsporum Canis ◽  
Diagnostic Studies ◽  
High Level ◽  
Mold And Yeast ◽  
The City ◽  
Mycological Laboratory

The spread of dermatophytosis among animals and humans in the localities of Ukraine in particular in the city of Kharkiv is due to the presence of range of the most susceptible animals. This information mainly concerns dogs and cats that can be affected by dermatophytosis and may be a reservoir of dermatophyte fungus. Particularly important is that domestic dogs and cats represent a significant epidemiological threat to the population. The purpose of the research was to monitor the morbidity of dermatophytosis in domestic dogs and cats in Kharkiv. Diagnostic studies of dermatophytosis morbidity level determination in domestic dogs and cats were performed complexly including the clinical and epizootic data, microscopic and mycological laboratory tests conducted according to generally accepted methods (Kovalenko et al., 2017; Sutton, Fothergill and Rinaldi, 2001). Dermatophytosis was diagnosed among 231 animals in the study of 1,277 domestic dogs kept by the inhabitants of the city of Kharkiv which was in 18.09% of samples. Dermatophytosis was also detected in 615 animals which constitutes 50.25% in the study of 1,124 cats. The cultures of dermatophytosis agent Microsporum canis were isolated from 126 dogs (18.98%) and 110 cats (40.74%). Trichophyton mentagrophytes cultures were isolated from 16 dogs (2.41%) and 16 cats (5.93%). The percentage of isolated cultures of mold and yeast-like fungi in the studies of sick dogs and cats were 38.55% and 22.25% respectively. Obtained results indicate quite high level of the dermatophytosis spreading among domestic dogs and cats in the city of Kharkiv.

scholarly journals Dermatofitosis humana en una comunidad con desigualdad social, Ayacucho 2018

2019 ◽  
Vol 27 (1) ◽  
Author(s):  
Serapio Romero Gavilán
Keyword(s):  
Trichophyton Rubrum ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Canis ◽  
Microsporum Gypseum

Trabajo de investigación desarrollado con el objetivo de conocer la frecuencia de la dermatofitosis humana en una comunidad con desigualdad social. Hipótesis: la dermatofitosis humana es una afección fúngica muy frecuente en comunidades con desigualdad social. Zona de estudio: comunidad con desigualdad social periurbana de la ciudad de Ayacucho. Tipo de estudio: no experimental. Diseño de estudio: descriptivo-transversal. Muestra: no probabilística, individuos con signos de afecciones compatibles a micosis superficial. Metodología: muestras de escamas de piel, pelos, fragmentos de uñas de pies y manos, escamas de planta, espacios interdigitales y otras partes de cuerpo, fueron tomadas con una hoja de bisturí de filo romo o con el borde de un portaobjetos previa desinfección con alcohol al 70% y colocadas en sobres de papel oscuro etiquetados, en el laboratorio de Epidemiología y Micología de la Universidad Nacional de San Cristóbal de Huamanga, se realizó la observación directa con KOH al 20% y la siembra en placas Petri conteniendo Agar Sabouraud y Agar selectivo para hongos patógenos, después de una incubación a 25ºC por hasta 14 días; las colonias coincidentes con dermatofitos fueron observados al microscopio para identificarlos. Resultados: se ha encontrado que 85/153 (55,5%) presentaron diversas formas de dermatofitosis, no se observó preferencia de la dermatofitosis humana con relación al género (p> 0,05), los factores asociados a la dermatofitosis determinados estadísticamente (p< 0,05) fueron la higiene, el piso de la vivienda y la crianza de animales, se han identificado las especies de Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum canis y Microsporum gypseum.

scholarly journals Development, Characterization, and Evaluation of Novel Broad-Spectrum Antimicrobial Topical Formulations from Cymbopogon martini (Roxb.) W. Watson Essential Oil

2018 ◽  
Vol 2018 ◽  
pp. 1-16 ◽  
Author(s):  
Negero Gemeda ◽  
Ashenif Tadele ◽  
Hirut Lemma ◽  
Biruktawit Girma ◽  
Getachew Addis ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Broad Spectrum ◽  
Chemical Constituents ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Canis ◽  
Skin Infections ◽  
Topical Formulations ◽  
Life Study

Background. Skin infections were the most frequently encountered of all infections and the 4th leading cause of nonfatal disease burden. Topical drugs have been used for the management of skin infections. The growing concern of drug resistance to the topical agents has warned the need for continuous development of novel drug. Essential oils are the best candidate for new drug with different mode of action and target as they are rich in chemical constituents. Objective. To evaluate and develop safe and effective topical antimicrobial formulations from essential oil of Cymbopogon martini. Method. Essential oil was extracted using hydrodistillation aerial part C. martini and topical formulations were prepared in five different semisolid bases. In vitro antimicrobial investigations were performed on essential oil and topical formulations. Skin sensitizations of the formulations were evaluated using guinea pig maximization. Results. The essential oil of C. martini has shown broad-spectrum antimicrobial potency against all tested organisms with MIC value ranging from 0.65 to 10 μg/ml. Absolute inhibitions of growth of fungi were observed against Trichophyton mentagrophytes and Trichophyton rubrum at concentrations above 1% of oil and against Microsporum canis and Trichophyton verrucosum at a concentration of 4% oil. Among topical formulations, the highest antimicrobial activity was recorded in hydrophilic ointment followed by macrogol blend ointment. The antimicrobial activity of oil was higher in fungal pathogen compared to bacteria. Gram positive bacteria were more sensitive than gram negative bacteria. Hydrophilic and macrogol blend ointment containing 5% oil did not produce any skin sensitization on guinea pigs. Conclusion. In conclusion, topical formulations of C. martini essential oil can be alternative topical agents with safe broad-spectrum activity for the treatment of skin disorder. Further studies should focus on shelf life study and clinical study of the product.

scholarly journals In VivoEvaluation ofGalla chinensisSolution in the Topical Treatment of Dermatophytosis

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Kai Sun ◽  
Xu Song ◽  
RenYong Jia ◽  
Zhongqiong Yin ◽  
Yuanfeng Zou ◽  
...  
Keyword(s):  
Trichophyton Mentagrophytes ◽  
Clinical Score ◽  
Skin Lesions ◽  
Positive Control ◽  
Microsporum Canis ◽  
Microsporum Gypseum ◽  
Pharmacological Effects ◽  
Mycological Cure ◽  
Candidate Drug ◽  
Cure Rates

Aim. Dermatophytosis is one of the main fungal diseases in humans and animals all over the world.Galla chinensis, a traditional medicine, has various pharmacological effects. The goal of this study was to evaluate the treatment effect ofGalla chinensissolution (GCS) on dermatophytosis-infected dogs (Microsporum canis,Microsporum gypseum, andTrichophyton mentagrophytes, resp.).Methods. The treatment effects of GCS were evaluated by mycological cure rates and clinical score comprised of three indices, including inflammation, hair loss, and lesion scale.Results. The results showed that, in the three models of dermatophytosis, GCS significantly (P<0.05) improved skin lesions and fungal eradication. GCS (10% and 5%) had higher efficacy compared to the positive control (Tujingpi Tincture). The fungal eradication efficacy exceeds 85% after treatment with GCS (10%, 5%, and 2.5%) on day 14.Conclusion. The GCS has antidermatophytosis effect in dogs, which may be a candidate drug for the treatment of dermatophytosis.

scholarly journals In vitro evaluation of the antifungal activity of Marco (Ambrosia arborescens Mill.) and Matico (Aristeguietia glutinosa Lam.) on the pathogenic fungi that cause dermatomycosis (ringworm)

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 559
Author(s):  
Tatiana de los Ángeles Mosquera Tayupanta ◽  
Sandra Elizabeth Ayala Valarezo ◽  
Tatiana Alexandra Vasquez Villareal ◽  
María Belén Montaluisa Álvarez
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Trichophyton Rubrum ◽  
Therapeutic Potential ◽  
Pathogenic Fungi ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Canis ◽  
The One ◽  
One Way Anova

Background: Currently, there is a trend towards using natural and ethnopharmacological species with therapeutic potential. This investigation evaluated the antifungal activity of two species in the Ecuadorian Andes, which are used in treating dermatomycosis: Ambrosia arborescens Mill. (Marco) and Aristeguietia glutinosa Lam. (Matico). Methods: We worked with seven concentrations (100 to 700ppm) of Ambrosia arborescens Mill. extract and ten concentrations (0.5 to 5%) of essential oil (EO) of Aristeguietia glutinosa Lam. on Trichophyton mentagrophytes ATCC 9533, Trichophyton rubrum ATCC 28188, Microsporum canis ATCC 36299 and Candida albicans ATCC 10231. The methodology used was a modified version of the Kirby-Bauer method, using diffusion in agar wells. Results: The Tukey test, after the one-way Anova, determined effective concentrations of EO: 5% for Trichophyton mentagrophytes, 4.5% for Trichophyton rubrum, 5% for Microsporum canis and 2% for Candida albicans. In the extracts, the concentration of 700ppm was used for Trichophyton mentagrophytes, Trichophyton rubrum, and 600ppm for Microsporum canis and Candida albicans. Conclusions: The evaluation of the antifungal activity of the Ambrosia arborescens extract showed inhibition in the studied dermatophytes in each one of the planted concentrations (100 to 700ppm). The evaluation of the antifungal activity of Aristeguietia glutinosa EO showed inhibition in the studied dermatophytes in each of the planted concentrations (0.5 to 5%).

scholarly journals European Hedgehogs (Erinaceus europaeus L.) as a Reservoir of Dermatophytes in Poland

2021 ◽  
Author(s):  
Sebastian Gnat ◽  
Dominik Łagowski ◽  
Mariusz Dyląg ◽  
Aneta Nowakiewicz
Keyword(s):  
Human Life ◽  
Primary Source ◽  
Trichophyton Mentagrophytes ◽  
Antifungal Drugs ◽  
Microsporum Canis ◽  
Missense Mutations ◽  
Erinaceus Europaeus ◽  
Trichophyton Erinacei ◽  
Trichophyton Benhamiae ◽  
European Hedgehog

AbstractThe European hedgehog (Erinaceus europaeus Linnaeus) frequently colonises areas located close to human life in cities, as these are more suitable nest sites offering an abundance of food and allowing avoidance of predators. However, urbanisation has a significant impact on the epidemiology of infectious diseases, including dermatophytoses, the primary source of which are wild animals. In this study, we determined the spectrum of dermatophytes isolated from the European hedgehog and assessed their susceptibility profile to antifungal drugs. Symptomatic and asymptomatic dermatophyte infections were observed in 7.7% and 8% of the 182 examined free-living hedgehogs, respectively. In the pool of the isolated dermatophyte strains, Trichophyton erinacei was dominant (29.9%), followed by Trichophyton mentagrophytes (17.9%), Trichophyton benhamiae (13.4%), Nannizzia gypsea (11.9%), Microsporum canis (10.4%), Nannizzia nana (7.5%), Paraphyton cookei (6.0%), and Nannizzia fulva (3.0%). Susceptibility tests revealed the highest activity of luliconazole and the lowest of activity fluconazole among the azole drugs applied. Although terbinafine generally exhibited high efficacy, two Trichophyton mentagrophytes isolates showed resistance to this drug (MIC = 2 µg/ml) resulting from missense mutations in the SQLE gene corresponding to the amino acid substitution Leu393Phe. Summarising, our study has also revealed that such wildlife animals as hedgehogs can be a reservoir of pathogenic human dermatophytes, including harmful strains resistant to commonly used antifungal drugs. Graphical Abstract

scholarly journals Los animales domésticos (perros y gatos) como reservorio fúngico

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
E. Piontelli L. ◽  
M. A. Toro S. M.
Keyword(s):  
Trichophyton Mentagrophytes ◽  
Microsporum Canis ◽  
Microsporum Gypseum ◽  
Phialophora Verrucosa ◽  
Exophiala Jeanselmei ◽  
Trichophyton Terrestre

En una investigación epidemiológica efectuada en la ciudad de Valparaíso y sus alrededores, entre los años 1985-86, se estudió mediante la técnica del tapete la presencia de hongos patógenos y oportunistas en 278 animales domésticos -191 perros y 87 gatos- sin lesiones clínicas aparentes de dermatofitosis en su pelaje.En los 191 perros, un 6,3% presentó dermatófitos zoofíficos (4 Trichophyton mentagrophytes y 8 Microsporum canis) y un 17,3% de dermatofitos geofílicos (2 Microsporum gypseum-fulvum complex, 6 Keratinomyces ajelloi y 24 Trichophyton terrestre). Los 87 gatos presentaron un 18,4% de zoofíficos (2 T. mentogrophytes, 14 M. canis) y un 12,6% de geofí1icos (1 M. gypseum-fulvum complex, 4 K. ajelloi y 4 T. terrestre).La mayor frecuencia de M. canis (16,1% en gatos y solo un 4,2% en perros}, indica que este agente es capaz de sobrevivir en el pelaje sin causar lesiones aparentes y debe considerarse la especie Zoo-antropofílica más común transmisible al hombre en la edad pediátrica.Entre los Onygenales e Hyphomycetes relacionados, la mayoría de los aislamientos corresponde a las especies de chrysosporium (30,9% del total de perros y 50,6% del total de gatos).Entre las especies fúngicas potencialmente patógenas, puede apreciarse una franca predominancia de los géneros Scopulariopsis, Altenaria y Aspergillus en perros y de Scopulariopsis y Alrernaria. en gatos. Phialophora verrucosa y Exophiala jeanselmei, se destacan como hallazgos interesantes

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