scholarly journals Efektivitas Low Density Lipoprotein dan Kuning Telur Ayam dan Puyuh pada Pengawetan Semen Ayam Merawang (EFFECTIVESS OF LOW DENSITY LIPOPROTEIN AND EGG YOLK FROM CHICKEN AND QUAIL ON MERAWANG SEMEN PRESERVATION)

2017 ◽  
Vol 18 (3) ◽  
pp. 345
Author(s):  
Magfira Magfira ◽  
Raden Iis Arifiantini ◽  
Ni Wayan Karniani Karja ◽  
Sri Darwati
Keyword(s):  
Sperm Motility ◽  
Cold Shock ◽  
Semen Quality ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Low Density ◽  
Sperm Abnormality ◽  
Semen Preservation ◽  
Chicken Egg Yolk

The successful of artificial insemination (AI) depends on the semen quality and extender. To minimize effect of cold shock during storage, extender is added with egg yolk. The objectives of this study were to compare the effectiveness of pure Low Density Lipoprotein (LDL) and egg yolk from domestic chicken and quail on motility and longevity of Merawang chicken sperm. The semen was collected by massage method from three Merawang roosters. Immediately after collection, semen was evaluated macroscopically and microscopically. Only semen demonstrated >70% motility and <20% sperm abnormality were used in this study. Semen divided into four aliquots and diluted with Lactate Ringer (LR) LDL chicken (RL-LDL-C), LR-LDL quail (LR-LDL-Q), LR- chicken Egg Yolk (LR-CEY), Ringer Lactate quail Egg Yolk (RL-QEY). Diluted semen than stored at 5oC. Sperm motility was examined twice a day and the longevity of sperm was determined every day until the sperm reach 0% motility. The motility of spermatozoa in the LR-LDL diluent differed from the sperm motility in the RL-QEY diluent at the 60th and 72th hour (P <0.05) poststorage. However, there was no difference in motility sperm in LR-LDL-C, RL-LDL-Q and RL-CEY. Additionally, there is no difference (P> 0.05) in spermatozoa longivity in the four diluents, with a range of longivities between 4.43 to 5.93 days. ABSTRAK Keberhasilan inseminasi buatan (IB) salah satunya bergantung pada kualitas semen dan pengencer yang digunakan. Dalam meminimalisir pengaruh cold shock saat penyimpanan, pengencer ditambahkan dengan kuning telur. Tujuan dari penelitian ini adalah untuk membandingkan efektivitas Low Density Lipoprotein (LDL) dan kuning telur yang berasal dari ayam kampung dan puyuh terhadap motilitas dan longivitas spermatozoa ayam. Koleksi semen dilakukan menggunakan metode pemijatan pada tiga ekor ayam merawang. Setelah semen dikoleksi, selanjutnya semen dievaluasi secara makroskopis dan mikroskopis. Semen yang menunjukkan motilitas 70% dan abnormalitas kurang dari 20% dibagi empat dan diencerkan menggunakan Ringer Laktat-LDLA (RL-LDLA), Ringer Laktat-(RL-LDLP), Ringer Laktatkuning telur ayam (RL-KTA), dan RL-kuning telur puyuh (RL-KTP). Semen yang telah diencerkan kemudian disimpan pada suhu 5oC. Motilitas spermatozoa diamati dua kali sehari sampai motilitas mencapai 0%. Motilitas spermatozoa dalam pengencer RL-LDLA berbeda dengan motilitas spermatozoa dalam pengencer RL-KTP pada jam ke-60 dan ke-72 (P<0.05) pascapenyimpanan. Akan tetapi tidak terdapat perbedaan motilitas spermatozoa dalam RL-LDLA, RL-LDLP dan RL-KTA. Longivitas spermatozoa dalam empat pengencer tidak terdapat perbedaan (P>0.05) dengan rentang longivitas antara 4,43 sampai 5,93 hari.

scholarly journals EFFECT OF EXTRACTED LOW DENSITY LIPOPROTEINS SUBSTITUTION ON SOME POST CRYOPRESERVATION SEMEN QUALITY OF HOLSTEIN BULLS

2016 ◽  
Vol 47 (1) ◽  
Author(s):  
Al-Ani & et al.
Keyword(s):  
Semen Quality ◽  
Plant Protection ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Low Density Lipoproteins ◽  
Control Group ◽  
Low Density ◽  
Sperm Plasma Membrane ◽  
The Individual

The aim of this study was to investigate the effect of replacement of low density Lipoproteins (LDL) in different concentrations  ,exctracted fram egg yolk in the Tris diluents of the Holestion Freizan bulls semen after cooling and different cryopreservation periods . This study was done during the period From September/2014 to March/2015 and a two parts periods . First from September to November /2014 to done the extraction of the low density Lipoprotein which it have be succifully  done at the viruses Laportary  which belonge to the diagnosis department /state Board of the plant protection /Ministry of Agriclturd (Abu_Gruiab/west of Baghdad).The LDL extraction was done by a procedure with different steps from fresh egg Yolk and the packeges were done after that and preservie it at Refregartor( 5c ,one week only) until used . The second period was began from December/2014 to March/2015 At the Artificial insemination Department /Abu_Gruab which belonge to the General company of the Animal Resources Department services/Ministry of Agricultural (25 Km west of Baghdad ). Four Freigan Bulls at 3_3.5 year old were used to collection the semen (1 eijaculate / bull / week) by artificial vagina . 1 millelitter of semen from each bull was taken and make pooling . Egg yolks was  added 20% to the control group it is substituted for the rest of the Low density lipoproteins and different concentrations , amounting to (8% LDL and 10% LDL and 12% LDL). And study the effect of these additions in recipes semen during different periods of Save (cooling 5c) and freeze after(48 hours and one month and two months and three months) .The results of the study reveled that the 8% LDL treatment (T1) group have a significant effect (p≤0.05) on the individual motility ,sperm plasma membrane and acrosome integrity in comparable with the control group at different  periods of the preservation.

scholarly journals PEMANFAATAN SARI KEDELAI SEBAGAI BAHAN PENGENCER PENGGANTI KUNING TELUR UNTUK KRIOPRESERVASI SPERMATOZOA HEWAN

2017 ◽  
Vol 36 (1) ◽  
pp. 21 ◽  
Author(s):  
Fitra Aji Pamungkas ◽  
Rantan Krisnan
Keyword(s):  
Vegetable Protein ◽  
Microbial Contamination ◽  
Cold Shock ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Ice Crystals ◽  
Low Density ◽  
Animal Products ◽  
Better Than

<p>The extenders commonly used for cryopreservation of spermatozoa are based on animal products such as egg yolk. Egg yolk contains cholesterol, phospholipid and low density protein which prevent the formation of ice crystals and protect the integrity of plasma membrane during cryopreservation process. Furthermore, egg yolk increased the risk of microbial contamination and related to the possible transmission of zoonotic agents. Soybeans are the products of vegetable protein is often used as an emulsifier in the production of food for humans and serves as a protection from the cold shock as well as low density protein in egg yolk. Several studies reported that soybean extenders for cryopreservation of spermatozoa produce the same quality or even better than the egg yolk based extenders and the optimal concentration of soy bean in the extenders forcryopreservation of spermatozoa at 0.8-1.5%.</p><p>Key words : Soy bean, egg yolk, extender, cryopreservation, spermatozoa</p><p align="center"><strong><br /></strong></p><p align="center"><strong>ABSTRAK</strong></p><p>Bahan pengencer yang biasa digunakan untuk kriopreservasispermatozoa didasarkan pada produk hewani seperti kuning telur. Kuning telur mengandung kolesterol, fosfolipid dan <em>low density protein</em>yang dapat mencegah pembentukan kristal es sehingga melindungi integritas membran plasma terhadap kejutan dingin selama proses kriopreservasi. Namun penggunaan kuning telur menimbulkan kekhawatiran terutamapotensi peningkatan kontaminasi mikroba dan agen penularan zoonosis. Kacang kedelai merupakan produk protein nabati yang sering digunakan sebagai pengemulsi dalam produksi makanan untuk manusia dan berfungsi sebagai pelindung dari kejutan dingin sama halnya <em>low density lipoprotein</em>pada kuning telur. Beberapa hasil penelitian melaporkan bahwa bahan pengencer kacang kedelaiuntuk kriopreservasi spermatozoa menghasilkan kualitas yang sama atau bahkan lebih bagus dibandingkan dengan bahan pengencer berbasis kuning telur dan konsentrasi kacang kedelai yang optimal pada bahan pengencer untuk kriopreservasispermatozoa sebesar 0,8-1,5%.</p><p>Kata kunci: Kacang kedelai, kuning telur, pengencer, kriopreservasi, spermatozoa</p>

Characterization of glycopeptides derived from the low-density lipoprotein of hen's egg yolk

1968 ◽  
Vol 46 (8) ◽  
pp. 983-988 ◽  
Author(s):  
J. Z. Augustyniak ◽  
W. G. Martin
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Low Density ◽  
Amide Nitrogen ◽  
Acetylneuraminic Acid ◽  
Hen’S Egg ◽  
Terminal Amino ◽  
Protein Moiety

Two glycopeptides (A and B) were isolated from pronase-digested vitellenin, the protein moiety of the low-density lipoprotein of hen's egg yolk. Aspartic acid was the only N-terminal amino acid of both glycopeptides but only A contained N-acetylneuraminic acid. A contained 55% hexose (mannose), 14% hexosamine, 12% N-acetylneuraminic acid, 0.71% amide nitrogen, and its molecular weight was 2.3 × 103. The corresponding values for B were 64, 17, 0.0, 0.75, and 2.0 × 103. Chemical analyses showed that B (and probably A) occurs in vitellenin with the heteropolysaccharide group bound N-glycosidically via the β-amide group of an asparaginyl residue. The indicated structure is R∙(NH)Asp∙Thr∙Ser∙(Ala, Gly, Val)∙Ile, where R, the heteropolysaccharide group, contains 2 hexosamine and 8 hexose residues.

scholarly journals The antioxidant effects of soybean lecithin- or low-density lipoprotein-based extenders for the cryopreservation of brown-bear (Ursus arctos) spermatozoa

2013 ◽  
Vol 25 (8) ◽  
pp. 1185 ◽  
Author(s):  
M. Alvarez-Rodríguez ◽  
M. Alvarez ◽  
L. Anel-López ◽  
C. Martínez-Rodríguez ◽  
F. Martínez-Pastor ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Ursus Arctos ◽  
Soybean Lecithin ◽  
Density Lipoprotein ◽  
Brown Bear ◽  
Egg Yolk ◽  
Type A ◽  
Low Density ◽  
Sperm Viability ◽  
Anti Oxidant

Egg yolk low-density lipoproteins (LDL) and soybean lecithin were evaluated as replacements for egg yolk in extenders used for the cryopreservation of brown-bear spermatozoa. The motility, viability and acrosomal status of post-thawed spermatozoa were analysed, and an egg-yolk extender was used as a control. The total antioxidant capacity of these extenders was tested. Soybean lecithin showed an effect that was dependent on the soybean concentration (2%, 3.5% and 5%) and source (Type A: 24% l-α-phosphatidylcholine, and Type B: 14–23% l-α-phosphatidylcholine). Only semen cryopreserved with 5% Type A soybean exhibited a sperm motility similar to that of semen cryopreserved in egg-yolk-based extender after thawing, although the sperm viability and acrosome status were not as high. Semen frozen in an extender containing LDL (10–15%) exhibited improved sperm viability in comparison with the control, but sperm motility was lower. The LDL-based extender exhibited a higher anti-oxidant activity than the egg-yolk extender and soy lecithin-based extenders. The extenders with higher anti-oxidant activity showed improvements in frozen sperm viability but lower semen motility. These results indicate that soybean lecithin did not have the same protective effect as egg yolk during the freezing of brown-bear spermatozoa but suggest that LDL (10–15%) could be a useful substitute for egg yolk in these extenders.

Sign in / Sign up

Export Citation Format

Share Document