scholarly journals EFFECT OF EXTRACTED LOW DENSITY LIPOPROTEINS SUBSTITUTION ON SOME POST CRYOPRESERVATION SEMEN QUALITY OF HOLSTEIN BULLS

2016 ◽  
Vol 47 (1) ◽  
Author(s):  
Al-Ani & et al.
Keyword(s):  
Semen Quality ◽  
Plant Protection ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Low Density Lipoproteins ◽  
Control Group ◽  
Low Density ◽  
Sperm Plasma Membrane ◽  
The Individual

The aim of this study was to investigate the effect of replacement of low density Lipoproteins (LDL) in different concentrations  ,exctracted fram egg yolk in the Tris diluents of the Holestion Freizan bulls semen after cooling and different cryopreservation periods . This study was done during the period From September/2014 to March/2015 and a two parts periods . First from September to November /2014 to done the extraction of the low density Lipoprotein which it have be succifully  done at the viruses Laportary  which belonge to the diagnosis department /state Board of the plant protection /Ministry of Agriclturd (Abu_Gruiab/west of Baghdad).The LDL extraction was done by a procedure with different steps from fresh egg Yolk and the packeges were done after that and preservie it at Refregartor( 5c ,one week only) until used . The second period was began from December/2014 to March/2015 At the Artificial insemination Department /Abu_Gruab which belonge to the General company of the Animal Resources Department services/Ministry of Agricultural (25 Km west of Baghdad ). Four Freigan Bulls at 3_3.5 year old were used to collection the semen (1 eijaculate / bull / week) by artificial vagina . 1 millelitter of semen from each bull was taken and make pooling . Egg yolks was  added 20% to the control group it is substituted for the rest of the Low density lipoproteins and different concentrations , amounting to (8% LDL and 10% LDL and 12% LDL). And study the effect of these additions in recipes semen during different periods of Save (cooling 5c) and freeze after(48 hours and one month and two months and three months) .The results of the study reveled that the 8% LDL treatment (T1) group have a significant effect (p≤0.05) on the individual motility ,sperm plasma membrane and acrosome integrity in comparable with the control group at different  periods of the preservation.

scholarly journals EXTRACTION AND LYOPHILIZATION LOW-DENSITY LIPOPROTEINS AND USED IT INSTEAD OF EGG YOLK IN SEMEN EXTENDER OF AWASSI RAMS AND THEIR INFLUENCE IN SPERM ABNORMALITIES STORE AT 5 ° C

2017 ◽  
Vol 48 (1) ◽  
Author(s):  
Al-Subaihawi & et al.
Keyword(s):  
Plant Protection ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Control Treatment ◽  
Control Group ◽  
Low Density ◽  
Awassi Sheep ◽  
Significant Difference ◽  
The Individual

This study was conducted  to explain the possibility of lyophilization of low – density lipoprotein (LDL)  that is extracted from the egg yolk to be used instead of the egg yolk in  a dilute  semen of Awassi sheep and its  impact on  the rate of deformities of sperm  after stored at 5 ° C for five days. The study  was conducted from September 2015 to February  2016 and in three stages. The first stage started from July until August 2015 in the laboratory of viruses, that is a related Department to the plant protection office. During this stag the (LDL) was extracted  through several sequential steps from fresh egg yolk, and purified and stored in Fastened and steriled vials which are refrigerated until the time of lyophilization. The second stage was conducted  in August  2015 in Al-Kindy company. During  this study the liquid (LDL) was  lyophilized  and kept in steriled and tightly- fostened viols and then was stored in under the  0°C . The  third stage was conducted in the college of Agriculture/ University of Baghdad. In this stage the semen was collected from four of the Awassi rams of the age (2 – 4) years .The semen samples were collected using artificial vagina and the pooled, to remove  the individual differences between the rams. Later on the sample was divided on the experimental treatments evenly 1 ml/ transaction using a dilute Tris with 1:10 dilution ratio. The different concentrations of LDL on the characteristics of semen have also been studied. However; the experiment was conducted from January to February 2016 and during which three concentrations of LDL have been used (3.2 , 4.8 and 6.2%) the transactions T1, T2 , and respectively and the control treatment (20% EY). The improve of the lyophilized LDL has been studied on the different kinds of distortions that effect the sperm at  preservation temperature 5°C, for five consecutive days. Results of the experiment to outweigh LDL transactions in the low proportion of the various distortions that happens to sperm in distortions head swollen to the control group in two days, third, fourth and zero, distortions on other days as well as these transactions excelled in deformation of the tail broken on the fourth day, and in other types of malformations observed failure the presence of significant difference between the control group as in the distortions severed head and tail severed tail twisted as well as the lack of distortions in other types as in distortions tail double moderation bulging and widget, we conclude from the foregoing the possibility of using LDL lyophilized in thinners semen because of its ability in maintaining semen and reduce some types of malformations as well as he did not adversely affect the sperm as it was similar to egg yolk in maintaining sperm if not better.

scholarly journals Efektivitas Low Density Lipoprotein dan Kuning Telur Ayam dan Puyuh pada Pengawetan Semen Ayam Merawang (EFFECTIVESS OF LOW DENSITY LIPOPROTEIN AND EGG YOLK FROM CHICKEN AND QUAIL ON MERAWANG SEMEN PRESERVATION)

2017 ◽  
Vol 18 (3) ◽  
pp. 345
Author(s):  
Magfira Magfira ◽  
Raden Iis Arifiantini ◽  
Ni Wayan Karniani Karja ◽  
Sri Darwati
Keyword(s):  
Sperm Motility ◽  
Cold Shock ◽  
Semen Quality ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Low Density ◽  
Sperm Abnormality ◽  
Semen Preservation ◽  
Chicken Egg Yolk

The successful of artificial insemination (AI) depends on the semen quality and extender. To minimize effect of cold shock during storage, extender is added with egg yolk. The objectives of this study were to compare the effectiveness of pure Low Density Lipoprotein (LDL) and egg yolk from domestic chicken and quail on motility and longevity of Merawang chicken sperm. The semen was collected by massage method from three Merawang roosters. Immediately after collection, semen was evaluated macroscopically and microscopically. Only semen demonstrated >70% motility and <20% sperm abnormality were used in this study. Semen divided into four aliquots and diluted with Lactate Ringer (LR) LDL chicken (RL-LDL-C), LR-LDL quail (LR-LDL-Q), LR- chicken Egg Yolk (LR-CEY), Ringer Lactate quail Egg Yolk (RL-QEY). Diluted semen than stored at 5oC. Sperm motility was examined twice a day and the longevity of sperm was determined every day until the sperm reach 0% motility. The motility of spermatozoa in the LR-LDL diluent differed from the sperm motility in the RL-QEY diluent at the 60th and 72th hour (P <0.05) poststorage. However, there was no difference in motility sperm in LR-LDL-C, RL-LDL-Q and RL-CEY. Additionally, there is no difference (P> 0.05) in spermatozoa longivity in the four diluents, with a range of longivities between 4.43 to 5.93 days. ABSTRAK Keberhasilan inseminasi buatan (IB) salah satunya bergantung pada kualitas semen dan pengencer yang digunakan. Dalam meminimalisir pengaruh cold shock saat penyimpanan, pengencer ditambahkan dengan kuning telur. Tujuan dari penelitian ini adalah untuk membandingkan efektivitas Low Density Lipoprotein (LDL) dan kuning telur yang berasal dari ayam kampung dan puyuh terhadap motilitas dan longivitas spermatozoa ayam. Koleksi semen dilakukan menggunakan metode pemijatan pada tiga ekor ayam merawang. Setelah semen dikoleksi, selanjutnya semen dievaluasi secara makroskopis dan mikroskopis. Semen yang menunjukkan motilitas 70% dan abnormalitas kurang dari 20% dibagi empat dan diencerkan menggunakan Ringer Laktat-LDLA (RL-LDLA), Ringer Laktat-(RL-LDLP), Ringer Laktatkuning telur ayam (RL-KTA), dan RL-kuning telur puyuh (RL-KTP). Semen yang telah diencerkan kemudian disimpan pada suhu 5oC. Motilitas spermatozoa diamati dua kali sehari sampai motilitas mencapai 0%. Motilitas spermatozoa dalam pengencer RL-LDLA berbeda dengan motilitas spermatozoa dalam pengencer RL-KTP pada jam ke-60 dan ke-72 (P<0.05) pascapenyimpanan. Akan tetapi tidak terdapat perbedaan motilitas spermatozoa dalam RL-LDLA, RL-LDLP dan RL-KTA. Longivitas spermatozoa dalam empat pengencer tidak terdapat perbedaan (P>0.05) dengan rentang longivitas antara 4,43 sampai 5,93 hari.

Effect of Turmeric and Ginger on Lipid Profile in Male Rats Exposed to Oxidative Stress

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Eman A. Al-Rekabi ◽  
Dheyaa K. Alomer ◽  
Rana Talib Al-Muswie ◽  
Khalid G. Al-Fartosi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Drinking Water ◽  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
Control Group ◽  
Very Low Density Lipoprotein ◽  
Low Density

The present study aimed to investigate the effect of turmeric and ginger on lipid profile of male rats exposed to oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to male rats. Methods: 200 mg/kg from turmeric and ginger were used, and the animals were treatment for 30 days. Results: the results showed a significant increase in cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), whereas it explained a significant decrease in high density lipoprotein (HDL) of male rats exposed to oxidative stress when compared with control group. the results showed a significant decrease in cholesterol, triglycerides, (LDL), (VLDL), whereas it explained a significant increase in (HDL) of rats treated with turmeric and ginger at dose 200 mg/kg when compared with male rats exposed to oxidative stress.

Characterization of glycopeptides derived from the low-density lipoprotein of hen's egg yolk

1968 ◽  
Vol 46 (8) ◽  
pp. 983-988 ◽  
Author(s):  
J. Z. Augustyniak ◽  
W. G. Martin
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Low Density ◽  
Amide Nitrogen ◽  
Acetylneuraminic Acid ◽  
Hen’S Egg ◽  
Terminal Amino ◽  
Protein Moiety

Two glycopeptides (A and B) were isolated from pronase-digested vitellenin, the protein moiety of the low-density lipoprotein of hen's egg yolk. Aspartic acid was the only N-terminal amino acid of both glycopeptides but only A contained N-acetylneuraminic acid. A contained 55% hexose (mannose), 14% hexosamine, 12% N-acetylneuraminic acid, 0.71% amide nitrogen, and its molecular weight was 2.3 × 103. The corresponding values for B were 64, 17, 0.0, 0.75, and 2.0 × 103. Chemical analyses showed that B (and probably A) occurs in vitellenin with the heteropolysaccharide group bound N-glycosidically via the β-amide group of an asparaginyl residue. The indicated structure is R∙(NH)Asp∙Thr∙Ser∙(Ala, Gly, Val)∙Ile, where R, the heteropolysaccharide group, contains 2 hexosamine and 8 hexose residues.

scholarly journals Effect of Whole Quail (Coturnix japonica) Egg Consumption on some Reproductive Parameters and Lipid Profile of Male Albino Rat (Rattus norvegicus)

2017 ◽  
Vol 9 (3) ◽  
pp. 315-320 ◽  
Author(s):  
Chidozie N. OKOYE ◽  
Samuel O. EKERE ◽  
Onyinyechukwu A. AGINA ◽  
Ikechukwu J. UDEANI ◽  
Chukwunonso K. EZEASOR
Keyword(s):  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Control Group ◽  
Low Density ◽  
Epididymal Sperm ◽  
Treatment Groups ◽  
Egg Consumption ◽  
Group A ◽  
The Mean

The present study evaluated the effect of whole egg consumption on the liver, testes, cauda epididymal sperm reserve and lipid profile of male rats. These evaluations were carried out on adult twenty (20) male albino rats, which were randomly selected into four groups of 5 rats each, designated groups A, B, C and D. Group A was the control group and received only equivalent volume of distilled water, while groups B, C and D received 0.25mg/kg, 0.5mg/kg; and 1.0mg/kg body weight of the quail egg respectively. Standard procedures were carried out in the tissue processing, cauda epididymal sperm reserve and in lipid profile determinations. On days 35 and 49, the mean serum total cholesterol value of group D was significantly lower than that of the control group. On day 35, the mean serum low density lipoprotein and high density lipoprotein (LDL and HDL cholesterol) values of all the treatment groups were significantly lower and higher than that of the control group, respectively. However, on days 49 and 63, the mean serum very low density lipoprotein (VLDL cholesterol) and triglyceride values of all the treatment groups were significantly higher than that of the control group. A significant increase in cadual epididymal sperm count (CESR) was recorded on day 63 at the mid and high doses. No obvious pathological lesions were observed in the histomorphology of the testes and liver when compared to the control. Therefore, whole quail egg consumption caused an increase in serum triglyceride and very low density lipoprotein concentration, and also improved fertility. In other words, prolonged consumption of quail egg should be done with caution as it may predispose one to cardiovascular disease.

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