scholarly journals Biological Control Stem Rot Diseases (Sclerotium Rolfsii) on Peanut (Arachis hypogaea L.) using Arbuscular Mycorrhizal Fungi (AMF) Indigenous

Akta Agrosia ◽  
2021 ◽  
Vol 24 (1) ◽  
pp. 25-31
Author(s):  
Fradilla Swandi ◽  
Eri Sulyanti ◽  
Darnetty Darnetty

Arbuscular Mycorrhizal Fungi (AMF) are known to have potential as biological agents controlling plant pathogens. This study aims to obtain indigenous AMF isolates that can suppress the attack of Sclerotium rolfsii which causes stem rot disease in peanut. The method used is an experimental method with a Completely Randomized Design with 5 treatments, namely A : AMF Glomus sp-3 + S. rolfsii; B: AMF Acaulospora sp + S. rolfsii; C: AMF Gigaspora sp + S. rolfsii; D: Combined AMF Glomus sp-3, Acaulospora sp, and Gigaspora sp + S. rolfsii; E: Without AMF + S. rolfsii (Control). Each treatment was repeated 5 times. The data were analyzed using Analysis of Variance (ANOVA) using the Statistix 8 program and the Least Significance Different (LSD) test at a 5% significance level. The results showed that the isolates of Acaulospora sp and Gigaspora sp were able to increase the resistance of peanut plants to stem rot disease (suppressing the incidence and severity of the disease) reaching 100%. Keywords: Arbuscular Mycorrhizal Fungi, indigenous, salicylic acid, Sclerotium rolfsii.

2014 ◽  
Vol 14 (2) ◽  
pp. 160-169
Author(s):  
Latifah Latifah ◽  
Hendrival Hendrival ◽  
Mihram Mihram

Association of antagonistic fungi Trichoderma harzianum Rifai and arbuscular mycorrhizal fungi for controlling the stem  rot disease on soybean.  The research objective was to study effect of application Trichoderma harzianum and arbuscular mycorrhizal fungi (AMF) in controling stem rot disease caused by Sclerotium rolfsii Sacc on soybean.   The research conducted in a Randomized Block Design (RBD) with five treatment levels: (1) without T. harzianum, without AMF, and without S. rolfsii [negative control (C-)], (2) without T. harzianum, without AMF, and S. rolfsii [positive control (C +)], (3) T. harzianum + S. rolfsii; (4) AMF + S. rolfsii, and (5) T. harzianum + AMF + S. rolfsii.  Parameters observed were basal stem rot disease development and yield components. The results showed that the application of a mixture of T. harzianum and AMF caused a longer disease incubation period (8.29 days) and the severity of stem base rot disease was 11.67% number of pods per plant (62.53 pods), the number of seeds per plant (225.05 seeds), and the weight of seeds per plant (27.73 g) were higher than that of the application of T. harzianum and AMF separately.


2020 ◽  
Vol 17 (4) ◽  
pp. 150
Author(s):  
O. TRISILAWATI

<p>ABSTRACT</p><p>The effects of several arbuscular mycorrhizal fungi (AMF) on thegrowth, nutrient uptake (nitrogen, phosphorus, and potassium), and acidphosphate activity of two promising numbers of Anacardium occidentaleseedling were evaluated. The experiment was conducted in the greenhouse of Indonesian Spices and Medicinal Crops Research Institute(BALITTRO) in 2002 for six months on a randomized design with twofactors and four replicates. First factor was isolate (six isolates of AMFand one control) consisting of : 1) control; 2) Glomus aggregatum; 3)Glomus etunicatum; 4) Mycofer; 5) Glomus sp.; 6) a mixture of Glomussp1, Glomus sp2, Glomus sp3, Glomus sp4, Glomus etunicatum,Gigaspora margarita, Gigaspora sp., and Enthropospora sp., and 7)Gigaspora sp. The second factor was two cashew promising numbers :Asembagus and Wonogiri. The results showed that AMF inoculationsignificantly affected the growth of cashew. Mycofer and mixed AMFwere more effective to Wonogiri promising number, while for Asembaguspromising number inoculation of mycofer was more effective. Inoculationwith mycofer to Asembagus promising number increased the uptake of Pand K nutrients by 65 and 53% while inoculation with mycofer and mixedAMF to Wonogiri promising number increased the uptake of N, P and Knutrients by 55, 38, and 17%, and by 18, 31, and 17%. Moreover, theAMF inoculation resulted in higher phosphatase activity. In mycorrhizalAsembagus promising number infected by mixed AMF, the increment ofphosphatase activity was 136.5%, whether in Wonogiri promising numberinfected by mycofer, the increment of phosphatase activity was 80% thancontrol.</p><p>Key words: Anacardium occidentale, promising number, growth,phosphatase activity</p><p>ABSTRAK</p><p>Pengaruh Pupuk Hayati Fungi Mikoriza Arbuskula(FMA) terhadap Pertumbuhan Benih Jambu Mete</p><p>Penelitian ini bertujuan untuk mengetahui pengaruh beberapa jenisfungi mikoriza arbuskula (FMA) terhadap pertumbuhan, serapan hara danaktivitas enzim fosfatase dari dua nomor harapan benih jambu mete(Anacardium occidentale). Penelitian dilakukan di rumah kaca Balittropada tahun 2002 selama 6 bulan, menggunakan rancangan acak yangterdiri dari dua faktor dan diulang empat kali. Faktor pertama adalahisolate (6 jenis isolat FMA dan satu kontrol) yaitu: 1). kontrol; 2). Glomusaggregatum; 3) Glomus etunicatum; 4). Mycofer; 5). Glomus sp.; 6).campuran dari Glomus sp1, Glomus sp2, Glomus sp3, Glomus sp4, Glomusetunicatum, Gigaspora margarita, Gigaspora sp., Enthropospora sp., dan7). Gigaspora sp. Faktor kedua adalah nomor harapan jambu mete, yaituAsembagus dan Wonogiri. Hasil penelitian mendapatkan bahwa inokulasiFMA berpengaruh nyata terhadap pertumbuhan jambu mete. Mycofer dancampuran FMA lebih efektif berpengaruh terhadap nomor harapanWonogiri, sedangkan mycofer lebih efektif berpengaruh terhadap nomorharapan Asembagus. Serapan hara P dan K pada nomor harapanAsembagus yang diinokulasi mycofer meningkat sebesar 65 dan 53%,sedangkan nomor harapan Wonogiri yang diinokulasi mycofer dancampuran FMA, serapan hara N, P, dan K meningkat masing-masingsebesar 55; 38; dan 17%, dan 18; 31; dan 17%. Selain itu, inokulasi FMAdapat meningkatkan aktivitas fosfatase akar jambu mete. Peningkatanaktivitas fosfatase akar jambu mete nomor harapan Asembagus yangterinfeksi oleh campuran FMA sebesar 136,5%, sedangkan pada nomorharapan Wonogiri yang terinfeksi mycofer, peningkatnnya sebesar 80%dibandingkan kontrol.</p><p>Kata kunci: Anacardium occidentale, nomor harapan, pertumbuhan,aktivitas fosfatase</p>


2020 ◽  
Vol 2 (2) ◽  
pp. 65-71
Author(s):  
Eri Sulyanti

This study was conducted to assess the biocontrol efficacy of arbuscular mycorrhizae fungi (AM Fungi) against stem rot disease caused by Sclerotium rolfsii Sacc. in peanut. The AM Fungi can be associated with almost all types of plants. The purpose of this study was to obtain isolates of arbuscular mycorrhiza fungi (AMF) as a potential biofungisida against Sclerotium rolfsii and can characterize the mechanism of the FMA in controlling S.rolfsii (salicylic acid) on peanut plants. The AM Fungi inoculant (40 spores g-1 in concentration) was introduced to peanut seedling (25 g plant-1 ) at planting time where as Sclerotium rolfsii  inoculated 30 days after planting time. The experiment was arranged in the completely randomized design (CRD), which is 7 treatment sand  repeated 10 times in the greenhouse  experiment. The data were analyzed using analysis of variance (ANOVA) using STAT program 8 and  the Tukey test at 5% significance level. The AM Fungi treatments showed significantly redused the percentage of disease severity in infected peanut plants around 34.28% - 57.15%  and longer incubation period, respectively. They increased root colonization  (20,00 - 46.67%) with a middle to high category. The AM Fungi C isolate (isolated from Solok county), and  the A isolate (isolated from Payakumbuh city)  were the best as a biocontrol against S rolfsii (57.15%), followed by isolate D (isolated from Padang Pariaman county) 54,30 %. They also increased Salicylic acid content 1,4 times (70.72 ppm) compared to control (49,59 ppm). It can be concluded that the application of AM Fungi as a biocontrol agent played an important role in plant resistance and exhibit greater potential to protect peanut plants against S. rolfsii.  


2016 ◽  
Vol 12 (2) ◽  
pp. 120
Author(s):  
Willie Samodra Laya ◽  
Moehansyah Moehansyah ◽  
Jamzuri Hadie

This study aimed to determine the effects of the provision of arbuscular mycorrhizal fungi (AMF), the provision of lime, and the provision of NPK fertilizer, and the interaction effect of the provision of Arbuscular mycorrhizal fungi (AMF), lime and NPK fertilizers in promoting the growth of pinto peanut in the soil media of post-mining land. The research method used was a completely randomized design (CRD) three-factor factorial with the first factor is the type of inoculant FMA (M) = 3 levels, the second factor is the provision of lime (K) = 3 levels, and the third factor is the NPK fertilizer (P) = 3 levels. These results indicated that the interaction between AMF Glomus sp. and NPK fertilizer dose of 1 gram/polybag can increase height increase pinto peanut plants for 34.16 % of the controls. The interaction between AMF Gigaspora sp. The lime dose of 50 % Al-dd and Fertilizers NPK dose of 1 gram/polybag can increase the growth of leaves pinto peanut plants at 108.33 % of the controls. The interaction between AMF Glomus sp. and NPK fertilizer dose of 2 grams/polybag can increase canopy and root biomass pinto peanut plants at 245.21 % of the controls. The interaction between AMF Glomus sp. and NPK fertilizer dose of 2 grams/polybag can increase canopy and root biomass pinto peanut plants at 245.21 % of the controls. Level relative mycorrhizal dependency (RMD) was influenced by the type of AMF plant inoculated host. Highest RMD shown in pinto peanut using AMF Glomus sp. is 31.99% at moderately dependent.


2017 ◽  
Vol 9 (1) ◽  
pp. 124-130 ◽  
Author(s):  
Khirood DOLEY ◽  
Mayura DUDHANE ◽  
Mahesh BORDE

Sclerotium rolfsii (Sacc.) is the causal agent of stem-rot in groundnut (Arachis hypogaea L.)crop. With the increase in demand for the groundnut, control of stem-rot efficiently by microbial strains is fast becoming inevitable as the conventional system of chemicals is degrading our ecosystem. This investigation here emphasizes on inoculation of arbuscular mycorrhizal fungi (AMF) and Trichoderma species for growth achievement and disease control. The present investigation showed that these microbial strains were found to be worth applying as they stimulated growth and decreased harmful effects of S. rolfsii (cv. ‘Western-51’). The increased biochemical parameters and antioxidant activities also indicated their defence related activities in groundnut plants. In spite of positive attributes meted out by these microbial strains towards groundnut crop, the interaction among AM fungi and Trichoderma species seemed to be less co-operative between each other which were noted when mycorrhizal dependency and percent root colonization were observed. However, in summary more practical application of low-input AM fungi along with Trichoderma species may be needed for the advancement of modern agricultural systems.


2010 ◽  
Vol 82 (3) ◽  
pp. 771-777 ◽  
Author(s):  
Edson L. Souchie ◽  
Rosario Azcón ◽  
Jose M. Barea ◽  
Eliane M.R. Silva ◽  
Orivaldo J. Saggin-Júnior

This study evaluated the synergism between several P-solubilizing fungi isolates and arbuscular mycorrhizal fungi to improve clover ( Trifolium pratense) growth in the presence of Araxá apatite. Clover was sown directly in plastic pots with 300g of sterilized washed sand, vermiculite and sepiolite 1:1:1 (v:v:v) as substrate, and grown in a controlled environment chamber. The substrate was fertilized with 3 g L-1 of Araxá apatite. A completely randomized design, in 8×2 factorial scheme (eight P-solubilizing fungi treatments with or without arbuscular mycorrhizal fungi)and four replicates were used. The P-solubilizing fungi treatments consisted of five Brazilian P-solubilizing fungi isolates (PSF 7, 9, 20, 21 and 22), two Spanish isolates ( Aspergillus niger and the yeast Yarowia lipolytica) and control (non-inoculated treatment). The greatest clover growth rate was recorded when Aspergillus niger and PSF 21 were co-inoculated with arbuscular mycorrhizal fungi. Aspergillus niger, PSF 7 and PSF 21 were the most effective isolates on increasing clover growth in the presence of arbuscular mycorrhizal fungi. Greater mycorrhizal colonization resulted in greater clover growth rate in most PSF treatments. PSF 7 was the best isolate to improve the establishment of mycorrhizal and rhizobia symbiosis.


2005 ◽  
Vol 85 (1) ◽  
pp. 31-40 ◽  
Author(s):  
C. Plenchette ◽  
C. Clermont-Dauphin ◽  
J. M. Meynard ◽  
J. A. Fortin

Market globalization, demographic pressure, and environmental degradation have led us to reconsider many of our current agricultural systems. The heavy use of chemical inputs, including fertilizers and pesticides, has resulted in pollution, decreased biodiversity in intensively-farmed regions, degradation of fragile agro-ecosystems, and prohibitive costs for many farmers. Low input sustainable cropping systems should replace conventional agriculture, but this requires a more comprehensive understanding of the biological interactions within agro-ecosystems. Mycorrhizal fungi appear to be the most important telluric organisms to consider. Mycorrhizae, which result from a symbiosis between these fungi and plant roots, are directly involved in plant mineral nutrition, the control of plant pathogens, and drought tolerance. Most horticultural and crop plants are symbiotic with arbuscular mycorrhizal fungi. Mycorrhizal literature is abundant, showing that stimulation of plant growth can be mainly attributed to improved phosphorous nutrition. Although the mycorrhizal potential of its symbiosis to improve crop production is widely recognized, it is not implemented in agricultural systems. There is an urgent need to improve and widely apply analytical methods to evaluate characteristics such as, relative field mycorrhizal dependency, soil mycorrhizal infectivity, and mycorrhizal receptivity of soil. Decreased use of fertilizers, pesticides, and tillage will favour arbuscular mycorrhizal fungi. However, shifting from one system to a more sustainable one is not easy since all components of the cropping system are closely linked. Different cases, from actual agricultural practices in different countries, are analyzed to highlight situations in which mycorrhizae might or might not play a role in developing more sustainable agriculture. Key words: Cropping systems, mycorrhizae, sustainability, technical itineraries, rotation


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