scholarly journals Enamel Hardness Differences After Topical Application Of Theobromine Gel And Casein Phosphopeptide-Amorphous Calcium Phosphate

2020 ◽  
Vol 10 (1) ◽  
pp. 5
Author(s):  
Tamara Yuanita ◽  
Nanik Zubaidah ◽  
Mifta Izha A R
Keyword(s):  
Citric Acid ◽  
Calcium Phosphate ◽  
Topical Application ◽  
Amorphous Calcium Phosphate ◽  
Acrylic Resin ◽  
Control Group ◽  
Tooth Erosion ◽  
Hardness Tester ◽  
Group I ◽  
Casein Phosphopeptide

Background: Most soft drinks contain citric acid / phosphoric acid with pH <3.5. Strong acids in the oral cavity cause tooth erosion. Tooth erosion leads to demineralization enamel. Remineralization can prevent demineralization process. One of the commercial remineralization agents is Casein Phosphopeptide-amorphous Calcium Phosphate(CPP-ACP) but it has low solubility and less affordable price. Therefore, a natural ingredients such as theobromine from cocoa extract need to be developed as remineralization agent. Objective: To determine enamel hardness differences due to topical application of theobromine gel and CPP-ACP. Method: incisive crowns were cut off (1x1) cm then planted in cylindrical acrylic resin. All samples were immersed with citric acid for 90 minutes then divided into 3 groups. Group I (control) was immersed with aquades (96 min), group II was applied with theobromin gel 200 mg/l (96 min), group III was applied with CPP-ACP (96 min). By using vickers hardness tester, enamel hardness be measured (15 sec) at 3 points. Results: There were significant differences between the control group, CPP-ACP group, and theobromine gel group. Conclusion: Enamel hardnesse with theobromine gel application is higher than CPP-ACP application..

scholarly journals Comparison of the Efficacy of Different Fluoride Varnishes on Dentin Remineralization During a Critical pH Exposure Using Quantitative X-Ray Microtomography

2018 ◽  
Vol 43 (6) ◽  
pp. E308-E316
Author(s):  
A Sleibi ◽  
A Tappuni ◽  
D Mills ◽  
GR Davis ◽  
A Baysan
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Mineral Content ◽  
Test Group ◽  
Control Group ◽  
Mineral Concentration ◽  
Voxel Size ◽  
X Ray ◽  
Casein Phosphopeptide

SUMMARY Objectives: The objective of this in vitro study was to quantify the amount of mineral change in demineralized dentin at pH 5.5 after the application of dental varnishes containing fluoride with casein phosphopeptide–amorphous calcium phosphate, fluoride and bioglass, or fluoride alone. Methods and Materials: A total of 12 extracted human sound mandibular premolar root samples were coated with an acid-resistant varnish, leaving a 2 × 3 mm window at the outer root surface. These root specimens were then randomly divided into four groups and separately subjected to the demineralizing cycle at a pH of 4.8 for five days to create artificial caries-like lesions in dentin. Subsequently, each sample was imaged using quantitative x-ray microtomography (XMT) at a 15-μm voxel size. Each test group then received one of the following treatments: dental varnish containing casein phosphopeptide–amorphous calcium phosphate and fluoride (CPP-ACP, MI varnish, GC Europe), bioglass and fluoride (BGA, Experimental, Dentsply Sirona), or fluoride alone (NUPRO, Dentsply Sirona), as well as a control group, which received no treatment. These samples were kept in deionized water for 12 hours. The thin layer of varnish was then removed. All samples including the nonvarnish group were subjected to the second demineralizing cycle at pH 5.5 for five days. The final XMT imaging was then carried out following the second demineralizing cycle. XMT scan was also carried out to varnish samples at 25 μm voxel size. The change in mineral concentration in the demineralized teeth was assessed using both qualitative and quantitative image analysis. Results: There was an increase in radiopacity in the subtracted images of all varnish groups; a significant increase in mineral content, 12% for the CPP-ACP and fluoride (p≤0.05 and p≤0.001), 25% BGA (p≤0.001), and 104% fluoride alone varnish (p≤0.001). There was an increase in the size of radiolucency in the lesion area with a significant decrease in mineral content in the nonvarnish group, 10% (p≤0.05 and p≤0.001). Conclusions: There was encouraging evidence of a remineralization effect following the application of dental varnish on dentin and also an observed resistance to demineralization during the acidic challenge in all cases. However, a dental varnish containing fluoride alone appeared to have a much greater effect on dentin remineralization when compared with CPP-ACP with fluoride and bioglass with fluoride.

Incipient Enamel Lesions Remineralization using Casein Phosphopeptide Amorphous Calcium Phosphate Cream with and without Fluoride: A Laser Fluorescence Study

2012 ◽  
Vol 36 (4) ◽  
pp. 253-355
Author(s):  
SS Bhat ◽  
KS Hegde ◽  
MA Habibullah ◽  
V Bernhardt
Keyword(s):  
Calcium Phosphate ◽  
Topical Application ◽  
Amorphous Calcium Phosphate ◽  
Positive Control ◽  
Negative Control ◽  
Laser Fluorescence ◽  
Fluorescence Study ◽  
In Vitro Investigation ◽  
Casein Phosphopeptide

The aim of this in vitro investigation was to evaluate and compare the incipient enamel lesions remineralized by topical application of casein phospho peptide amorphous calcium phosphate (CPP-ACP) cream with and without fluoride. Method: Sixty caries free teeth were used in the study. They were divided into four groups as positive control, negative control and two experimental groups.The samples were demineralized and then remineralized using a CPP-ACP Cream with and without fluoride. The remineralization was evaluated at 7, 14 and 21 days using laser fluorescence. Results of this study showed that the laser fluorescence readings of test samples for remineralization were very highly significant at 14 and 21 days (p &lt; 0.001). Conclusions: The degree of remineralization achieved between CPP-ACP and CPP-ACP with fluoride was statistically significant ( p = 0.040) at 21 days.

Comparative Evaluation of Casein Phosphopeptide - Amorphous Calcium Phosphate and Fluoride in Managing Early Caries Lesions

2019 ◽  
Vol 70 (10) ◽  
pp. 3746-3749
Author(s):  
Livia Bobu ◽  
Alice Murariu ◽  
Gabi Topor ◽  
Adrian Beznea ◽  
Roxana Vasluianu
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Control Group ◽  
Invasive Treatment ◽  
Treatment Groups ◽  
Non Invasive ◽  
Group 4 ◽  
Group 2 ◽  
Casein Phosphopeptide ◽  
Caries Lesions

The aim of the present study was to assess the remineralizing potential of 10% casein phosphopeptide-amorphous calcium phosphate complex (CPP-ACP) -Recaldent used alone or with fluoride (CPP-ACFP) as compared to fluoride mouthrinse for the non-invasive treatment of occlusal non-cavitary caries lesions in vivo.A total of eighty subjects were randomly assigned to four groups, i.e. group 1 - 10% CPP-ACP, group 2- 10% CPP-ACP + 0.2% NaF, group 3- 0.05% NaF mouthrinse and group 4 �control. The lesions were assessed using visual examination and DIAGNOdent measurements. After 12 weeks of using the corresponding product, the results showed that the highest decrease in fluorescence was produced by CPP-ACFP (from 16.28�1.40 to 6.53�0.59, p=0.002), followed by CPP-ACP (from 15.70�1.82 to 8.18�0.70, p=0.004) and fluoride mouthrinse (from 14.32�1.72 to 7.88�0.68, p=0.005), significantly higher than in the control group (p[0.05). No significant differences were found between the three treatment groups.

scholarly journals Do enamel microabrasion and casein phosphopeptide-amorphous calcium phosphate affect shear bond strength of orthodontic brackets bonded to a demineralized enamel surface?

2011 ◽  
Vol 82 (1) ◽  
pp. 36-41 ◽  
Author(s):  
Asli Baysal ◽  
Tancan Uysal
Keyword(s):  
Calcium Phosphate ◽  
Bond Strength ◽  
Amorphous Calcium Phosphate ◽  
Shear Bond Strength ◽  
Enamel Surface ◽  
Orthodontic Brackets ◽  
Group I ◽  
Demineralized Enamel ◽  
Significant Difference ◽  
Casein Phosphopeptide

Abstract Objective: To evaluate and compare the effects of enamel microabrasion, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), and their combination on the shear bond strength (SBS) of orthodontic brackets bonded to demineralized enamel surface. Materials and Methods: One hundred human first premolar teeth were randomly allocated to one of five groups. Group I was considered as the control of other groups. For the remaining groups, demineralization was performed via solutions. In group II, brackets were directly bonded to the demineralized enamel surface. CPP-ACP was applied in group III, microabrasion was performed in group IV, and both microabrasion and CPP-ACP application were performed in group V. The specimens were tested for SBS. Bond failures were scored according to the Adhesive Remnant Index (ARI). Analysis of variance and Tukey tests were used to compare the SBS of the groups. ARI scores of the groups were evaluated with a G-test. The statistical significance was set at P &lt;. 05 level. Results: Statistically significant difference was found among the five investigated groups (F  =  111.870; P &lt; .001). The SBS of groups II and IV were significantly lower than the other groups. No statistically significant difference was found among groups I (control; mean 24.1 ± 4.1 MPa), III (mean 22.0 ± 3.6 MPa), and V (mean 24.3 ± 1.9 MPa). Microabrasion and combination with CPP-ACP showed higher SBS compared to the control group. The differences between ARI scores of the groups were statistically significant (P &lt; .001). Conclusion: CPP-ACP pretreatment, microabrasion of the enamel, and the combination of these two methods improve the bonding to demineralized enamel.

Use of Laser fluorescence and Scanning Electron Microscope to Evaluate Remineralization of Incipient Enamel Lesions Remineralized by Topical Application of Casein Phospho Peptide Amorphous Calcium Phosphate (CPP-ACP) Containing Cream

2008 ◽  
Vol 32 (3) ◽  
pp. 201-206 ◽  
Author(s):  
Deepika. Pai ◽  
Sham Bhat ◽  
Abhay Taranath ◽  
Sharan Sargod ◽  
Vinita Pai
Keyword(s):  
Electron Microscope ◽  
Calcium Phosphate ◽  
Scanning Electron Microscope ◽  
Topical Application ◽  
Amorphous Calcium Phosphate ◽  
In Vitro Study ◽  
Laser Fluorescence ◽  
Casein Phosphopeptide ◽  
Scanning Electron

The aim of this in vitro study was to evaluate the remineralization of incipient enamel lesions by the topical application of Casein PhosphoPeptide-Amorphous Calcium Phosphate (CPP-ACP) using laser fluorescence and scanning electron microscope. Sixty caries free extracted teeth were used in the study. Forty teeth were used as test samples, ten as positive and ten as negative controls. The samples were demineralized and then remineralized by the topical application of CPP-ACP for a period of 14 days. The remineralization was evaluated with the use of laser fluorescence and scanning electron microscope (SEM). The results of this study showed that the laser fluorescent readings of test samples after remineralization were highly significant (p &lt;0.001). A significant number of test samples observed under SEM showed high scores of remineralization.

scholarly journals Effect of Casein Phosphopeptide-Amorphous Calcium Phosphate and Three Calcium Phosphate on Enamel Microhardness

2017 ◽  
Vol 18 (7) ◽  
pp. 583-586 ◽  
Author(s):  
En HR Haghgou ◽  
Roza Haghgoo ◽  
Mohamad R Roholahi ◽  
Zahra Ghorbani
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Artificial Saliva ◽  
Testing Machine ◽  
Third Molar ◽  
Negative Control ◽  
Control Group ◽  
Healthy Human ◽  
Significant Difference ◽  
Casein Phosphopeptide

ABSTRACT Aim This study aims to investigate the effect of casein phosphopeptide-amorphous calcium phosphate and three calcium phosphate (CPP-ACP and TCP) on increasing the microhardness of human enamel after induction of erosion. Materials and methods A total of 26 healthy human-impacted third molar teeth were chosen, and their hardness measured using a microhardness testing machine. The samples were immersed in Coca Cola (pH = 4.7) for 8 minutes. Then, microhardness was measured again, and these samples were randomly divided into four groups (two control groups and two experimental groups). (1) Negative control group: Artificial saliva was used for 10 minutes, (2) positive control group: Fluoride gel was used for 10 minutes, (3) β-TCP group: TCP was used for 10 minutes, (4) CCP-ACP group: CCP-ACP was used for 10 minutes. The final microhardness of those samples was measured, and the changes in microhardness of teeth within group and between groups were analyzed using the paired and analysis of variance tests respectively. Results were considered statistically significant at a level of p < 0.05. Results No significant difference was observed in microhardness between CPP-ACP group and TCP group (p = 0.368) during the time microhardness significantly dropped after soaking in soda. Conclusion Casein phosphopeptide-amorphous calcium phosphate and TCP increased the microhardness of teeth. The increase in hardness in the TCP group was higher than in the CPP-ACP group, but this difference was not significant (p = 0.36). Clinical significance Casein phosphopeptide-amorphous calcium phosphate and TCP can affect the remineralization of erosive lesions. How to cite this article Haghgou EHR, Haghgoo R, Roholahi MR, Ghorbani Z. Effect of Casein Phosphopeptide-Amorphous Calcium Phosphate and Three Calcium Phosphate on Enamel Microhardness. J Contemp Dent Pract 2017;18(7):583-586.

Comparative Evaluation of Casein Phosphopeptide - Amorphous Calcium Phosphate and Fluoride in Managing Early Caries Lesions

2019 ◽  
Vol 70 (10) ◽  
pp. 3746-3749
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Control Group ◽  
Invasive Treatment ◽  
Treatment Groups ◽  
Non Invasive ◽  
Group 4 ◽  
Group 2 ◽  
Casein Phosphopeptide ◽  
Caries Lesions

The aim of the present study was to assess the remineralizing potential of 10% casein phosphopeptide-amorphous calcium phosphate complex (CPP-ACP) -Recaldent used alone or with fluoride (CPP-ACFP) as compared to fluoride mouthrinse for the non-invasive treatment of occlusal non-cavitary caries lesions in vivo.A total of eighty subjects were randomly assigned to four groups, i.e. group 1 - 10% CPP-ACP, group 2- 10% CPP-ACP + 0.2% NaF, group 3- 0.05% NaF mouthrinse and group 4 -control. The lesions were assessed using visual examination and DIAGNOdent measurements. After 12 weeks of using the corresponding product, the results showed that the highest decrease in fluorescence was produced by CPP-ACFP (from 16.28±1.40 to 6.53±0.59, p=0.002), followed by CPP-ACP (from 15.70±1.82 to 8.18±0.70, p=0.004) and fluoride mouthrinse (from 14.32±1.72 to 7.88±0.68, p=0.005), significantly higher than in the control group (p<0.05). No significant differences were found between the three treatment groups. Keywords: CPP-ACP, CPP-ACFP, fluoride mouthrinse, remineralization

scholarly journals Effect of Acidulated Phosphate Fluoride and Casein Phosphopeptide–Amorphous Calcium Phosphate Application on Shear Bond Strength of Orthodontic Brackets

2008 ◽  
Vol 78 (1) ◽  
pp. 129-133 ◽  
Author(s):  
Defne Keçik ◽  
Sevi Burçak Çehreli ◽  
Çağla Şar ◽  
Bahtiyar Ünver
Keyword(s):  
Calcium Phosphate ◽  
Bond Strength ◽  
Amorphous Calcium Phosphate ◽  
Shear Bond Strength ◽  
Control Group ◽  
Significant Difference ◽  
Acid Etch ◽  
Bond Strengths ◽  
Group 2 ◽  
Casein Phosphopeptide

Abstract Objective: To evaluate the effect of a recently introduced prophylactic agent, casein phosphopeptide–amorphous calcium phosphate (CPP-ACP), on shear bond strength of brackets and compare it with the effect of acidulated phosphate fluoride (APF). Materials and Methods: Forty-eight freshly extracted mandibular bovine incisors were used. Teeth were randomly divided into four groups (n = 12) as follows: group 1 served as control, and no pretreatment was performed on the enamel; group 2, enamel was treated with 1.23% APF and CPP-ACP, respectively; group 3, enamel was treated with CPP-ACP; and group 4, enamel was treated with 1.23% APF for 4 minutes. In all groups, brackets were bonded using a conventional acid-etch and bond system (Transbond XT, 3M Unitek, Monrovia, Calif). Bonded specimens were first stored in deionized water at 37°C for 24 hours, subjected to thermal cycling for 1000 cycles, and further stored in distilled water for 6 weeks before debonding procedures. After debonding, teeth and brackets were examined under a stereomicroscope at 10× magnification for any adhesive remaining, in accordance with the modified adhesive remnant index. Results: The shear bond strengths of all experimental groups were significantly higher than that of the control group (P &lt; .01). There was no significant difference between the shear bond strengths of the experimental groups (P &gt; .05). Conclusion: The use of CPP-ACP either alone or combined with APF could be considered as an alternative prophylactic application in orthodontic practice since it did not compromise bracket bond strength.

scholarly journals Effect of erbium, chromium: yttrium, scandium, gallium, garnet laser and casein phosphopeptide-amorphous calcium phosphate on surface micro-hardness of primary tooth enamel

2014 ◽  
Vol 08 (03) ◽  
pp. 402-406 ◽  
Author(s):  
Priya Subramaniam ◽  
Annu Pandey
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Tooth Enamel ◽  
Group Iv ◽  
Primary Tooth ◽  
Micro Hardness ◽  
Group Iii ◽  
Ysgg Laser ◽  
Group I ◽  
Casein Phosphopeptide

ABSTRACT Objective: The aim was to evaluate the effect of Er, Cr: YSGG laser and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) on surface micro-hardness of primary tooth enamel. Materials and Methods: A total of 30 freshly extracted caries free primary anterior teeth were cleaned and stored in 1% thymol. Teeth were embedded in acrylic resin such that only their buccal surfaces were exposed and were divided into four groups. Group I: Five intact teeth (negative control). The remaining 25 teeth were immersed for 30 min in 1% citric acid for demineralization. Group II: Five demineralized teeth (positive control), Group III: CPP-ACP (GC tooth mousse-GC International, Itabashi-Ku, Tokyo, Japan) application and Group IV: Etching using Er, Cr: YSGG laser + CPP-ACP application. Groups III and IV were subjected to pH cycling for 5 days. Surface micro-hardness of all the teeth was measured using Brinell hardness tester (Fuel Instruments and Engineers Pvt. Ltd.). Data were analyzed using ANOVA. Results: Mean surface micro-hardness of Groups I and II were 177.43 kgf/mm2 and 164.86 kgf/mm2, respectively. Group IV showed a higher mean surface micro-hardness (230.68 kgf/mm2) compared with that of Group III (190.28 kgf/mm2). In comparison to all other groups, laser etching prior to CPP-ACP application increased surface micro-hardness significantly (P < 0.001). Conclusion: Laser irradiation of primary teeth followed by CPP-ACP application increased surface micro-hardness of enamel.

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