scholarly journals The activity of polyclonal IgY derived from Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in inhibiting colonization of Fusobacterium nucleatum and Streptococcus sanguinis

2019 ◽  
Vol 52 (2) ◽  
pp. 81
Author(s):  
Oktaviani Suci Lestari ◽  
Rini Devijanti Ridwan ◽  
Tuti Kusumaningsih ◽  
S. Sidarningsih
Keyword(s):  
Porphyromonas Gingivalis ◽  
Fusobacterium Nucleatum ◽  
Egg Yolk ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Plaque Formation ◽  
Surface Element ◽  
Dilution Ratio ◽  
Precipitation Line ◽  
Streptococcus Sanguinis ◽  
Made In

Background: Fusobacterium nucleatum (F. nucleatum) and Streptococcus sanguinis (S. sanguinis) play a role in dental plaque formation which leads to periodontitis. Immunoglobulin Y (IgY) is present in both serum and egg yolk and can bind to the surface components of bacteria. F. nucleatum and S. sanguinis feature the same type of IV pili as Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). Saliva binding protein (SsaB) in S. sanguinis is a FimA homolog. FimA constitutes a surface element of Porphyromonas gingivalis (P. gingivalis). F. nucleatum and P. gingivalis possess the same outer membrane protein (OMP) molecular mass. Purpose: The study aimed to determine the activity of A. actinomycetemcomitans and P. gingivalis polyclonal IgY present in serum and egg yolk that can inhibit colonization of F. nucleatum and S. sanguinis. Methods: IgY samples were diluted with phosphate buffer saline (PBS). Several holes were made in the nutrient medium with 10 μl antigen (F. nucleatum/S. sanguinis) being inserted into the center hole. 10 μl PBS, 1:1, 1:2, 1:4, 1:8, 1:16 A. actinomycetemcomitans or P. gingivalis polyclonal IgY were subsequently introduced into the surrounding holes. The results of incubation at 37°C were observed after 24-48 hours. Kruskal Wallis and MannWhitney tests were administered to analyse the data. Results: A. actinomycetemcomitans and P. gingivalis polyclonal IgY groups in serum showed a precipitation line at dilution ratios of 1:1 and 1:2, whereas in egg yolk this occurred only at a 1:1 dilution ratio with F. nucleatum and S. sanguinis bacteria in this study. No significant differences were evident between each dilution (p>0.05) and none existed between serum and egg yolk (p>0.05). Conclusion: IgY polyclonal of A. actinomycetemcomitans and P. gingivalis in both serum and egg yolk initiate activities that can inhibit colonization of F. nucleatum and S. sanguinis.

scholarly journals Analysis of anti-Streptococcus sanguinis IgY ability to inhibit Streptococcus sanguinis adherence

2018 ◽  
Vol 51 (1) ◽  
pp. 33
Author(s):  
Suryani Hutomo ◽  
Heni Susilowati ◽  
Dewi Agustina ◽  
Widya Asmara
Keyword(s):  
Dental Plaque ◽  
Systemic Disease ◽  
Initial Step ◽  
Plaque Formation ◽  
Optimal Concentration ◽  
Bacterial Adherence ◽  
Tooth Surface ◽  
Precipitation Line ◽  
Streptococcus Sanguinis ◽  
Significant Difference

Background: Streptococcus sanguinis (S. sanguinis), an oral commensal bacterium, is often implicated in infective endocarditis. Its adherence to the tooth surface is the initial step in dental plaque formation. In addition to the important role of S. sanguinis in systemic disease and antimicrobial resistance, it is necessary to develop methods to control dental plaque formation. Immunoglobulin Y (IgY) has been used to prevent bacterial infection. Purpose: The purpose of this study is to analyze the ability of anti-S. sanguinis IgY antibodies to inhibit S. sanguinis adherence to hydroxyapatite (HA) discs as a model of the tooth surface. Methods: Antibodies were produced by immunizing hens with S. sanguinis suspension. Boosters were given three times following the first injection. An agar gel precipitation test (AGPT) was used to detect the presence of anti-S. sanguinis IgY. A bacterial adherence assay was performed twice to analyze the ability of IgY and the optimal concentration required to inhibit bacterial adherence. Results: The formation of a precipitation line using AGPT confirmed the presence of the antibody. In addition, it was shown that the anti-S. sanguinis IgY antibody could inhibit bacterial adherence to HA. Statistical analysis using One-way ANOVA revealed a significant difference in the optical density (OD) value between the groups (p<0.05). The results of electron microscopy scanning confirmed the quantitative analysis by means of a bacterial adherence test. Conclusion: Anti-S. sanguinis IgY has the ability to inhibit adherence of S. sanguinis to HA discs at an optimal concentration of 30%. The inhibitive effect was stronger in the presence of saliva.

Detección de bacterias periodontopáticas en niños con anemia de Fanconi

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Karine Lyko ◽  
Carmem Bonfimz ◽  
Elaine Machado Benelli ◽  
Cassius Carvalho Torres-Pereira ◽  
José Miguel Amenábar
Keyword(s):  
16S Rrna ◽  
Porphyromonas Gingivalis ◽  
Fusobacterium Nucleatum ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Treponema Denticola ◽  
Los Grupos

Objetivo: Comparar la prevalencia de cuatro bacterias periodontopáticas incluyendo Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Fusobacterium nucleatum y Treponema denticola en muestras de placa supragingival de niños con y sin anemia de Fanconi. Material y métodos: Muestras de placa supragingival fueron colectadas en 71 personas con edades entre 6-18 años de edad. Las muestras se dividieron en tres grupos: anemia de Fanconi pre-trasplante (n= 25), anemia de Fanconi post-trasplante (n=23) y control (n=24). Las bacterias se identifi on mediante la reacción en cadena de la polimerasa para amplifi in vitro al gen codifi 16S rRNA. Resultados: El A. actinomycetemcomytans sólo fue encontrado en una muestra del grupo pretrasplante. El microorganismo P. gingivalis se identificó en una muestra del grupo pre-trasplante y en una del grupo post-trasplante. El T. denticola se encontró únicamente en dos muestras del grupo pre-trasplante. El microorganismo F. nucleatum se observó en todos los grupos. La presencia de los microorganismos varió del 30% en el grupo control al 58% en el grupo Prétrasplante. No fueron encontradas diferencias estadísticas entre los grupos. Conclusión: Los resultados del estudio sugieren que las alteraciones sistémicas encontradas en los individuos con AF no afectan la prevalencia de las cuatro bacterias analizadas.

scholarly journals Lipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of mice

2021 ◽  
Vol 37 (1) ◽  
Author(s):  
Ae Ri Kim ◽  
Yun Kyong Lim ◽  
Joong-Ki Kook ◽  
Eun-Jung Bak ◽  
Yun-Jung Yoo
Keyword(s):  
Flow Cytometry ◽  
Porphyromonas Gingivalis ◽  
Osteoclast Differentiation ◽  
Fusobacterium Nucleatum ◽  
Pathological Changes ◽  
Air Pouch ◽  
E Coli ◽  
Differentiation Factor ◽  
Osteoclast Differentiation Factor

AbstractIncreases of neutrophils and osteoclasts are pathological changes of periodontitis. RANKL is an osteoclast differentiation factor. The effect of periodontopathogen LPS on RANKL-expressing neutrophils has not been clarified yet. We evaluated numerical changes of RANKL-expressing neutrophils in air pouches of mice injected with LPSs of Fusobacterium nucleatum and Porphyromonas gingivalis. Mice with air pouches were assigned into saline (C)-, E. coli LPS- (Ec LPS)-, F. nucleatum LPS (Fn LPS)-, P. gingivalis LPS (Pg LPS)-, and Fn LPS and Pg LPS (Fn + Pg LPS)-injected groups. CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils in blood and air pouch exudates were determined by flow cytometry. In blood, compared to the C group, the Fn LPS group showed increases of CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils whereas the Pg LPS group showed no significant differences. These increases in the Fn LPS group were not different to those in the Ec LPS group. In exudates, Fn LPS and Pg LPS groups showed increases of CD11b+Ly6G+ neutrophils and CD11b+Ly6G+RANKL+ neutrophils compared to the C group. Increased levels in the Fn LPS group were not different to those in the Ec LPS group, but Pg LPS group was lower than those in the Ec LPS group. In blood and exudates, the Fn + Pg LPS group showed no difference in levels of these neutrophils compared to the Ec LPS group. LPSs of F. nucleatum and P. gingivalis increased RANKL-expressing neutrophils although the degrees of increases were different. These suggest that periodontopathogen LPS can act as a stimulant to increase RANKL-expressing neutrophils.

Профилактика воспалительных заболеваний ротовой полости у человека с помощью комбинации пробиотических штаммов Streptococcus salivarius BLIS K12 и BLIS M18 в наземном изоляционном эксперименте «SIRIUS-19»

2021 ◽  
Author(s):  
Л. Прокопович
Keyword(s):  
Porphyromonas Gingivalis ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Treponema Denticola ◽  
Prevotella Intermedia ◽  
Streptococcus Salivarius

Изучены синергия штаммов саливарного стрептококка BLIS K12 и M18 в ходе профилактики воспаления ротоглотки, а также возможности инвазии протективной микрофлоры в процессе межличностного общения в замкнутом пространстве искусственной среды обитания. Впервые в клинической практике изучено влияние пробиотиков на основе саливарного стрептококка на пародонтопатогены I и II порядков: Prevotella intermedia, Tanerella forsythia, Treponema denticola, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis. Полученные результаты можно расценивать как положительные. По результатам наземного эксперимента «SIRIUS-19» планируется проводить закупки для космических экспериментов с шифрами «Пародонт» и «Пародонт-ЛОР».

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