scholarly journals Simultaneous Determination Of Sodium Benzoate And Sodium Cyclamate In Soft Drink Using High Performance Liquid Chromatography

2018 ◽  
Vol 3 (2) ◽  
pp. 62
Author(s):  
Ariya Wijaya Putra ◽  
Asri Darmawati ◽  
Juniar Mochtar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Sodium Benzoate ◽  
High Performance ◽  
Soft Drink ◽  
Correlation Coefficients ◽  
Optimum Conditions ◽  
Sodium Cyclamate

Background: Sodium benzoate and sodium cyclamate are foods additives generally used as preservative and artificial sweeteners, respectively. Monitoring of sodium benzoate and sodium cyclamate need appropriate analytical method. Objective: The main objective of this study was to gain the optimum conditions for simultaneous determination of sodium benzoate and sodium cyclamate in soft drink by High Performance Liquid Chromatography. Methods: Measurement of the additives was performed in µ-Bondapak RP18 column using methanol:phosphate buffer of pH 4 (42:58) as mobile phase with flow rate of 1 mL/minute. Results: It has shown that correlation coefficients (r) of sodium benzoate and sodium cyclamate was 0.9997 and 0.9991 respectively. Recoveries of the two analyst were acceptable. Conclusions: Five out of nine samples tested contained sodium cyclamate higher than the recommended concentration. All sodium benzoate containing samples were within the recommended concentration.

scholarly journals Simultaneous Analysis of Ursolic Acid and Oleanolic Acid in Guava Leaves Using QuEChERS-Based Extraction Followed by High-Performance Liquid Chromatography

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Chang Xu ◽  
Yiyi Liao ◽  
Chunyan Fang ◽  
Makoto Tsunoda ◽  
Yingxia Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Ursolic Acid ◽  
Oleanolic Acid ◽  
High Performance ◽  
Correlation Coefficients ◽  
Guava Leaves ◽  
Guava Leaf

In this paper, a novel method of QuEChERS-based extraction coupled with high-performance liquid chromatography has been developed for the simultaneous determination of ursolic acid (UA) and oleanolic acid (OA) in guava leaves. The QuEChERS-based extraction parameters, including the amount of added salt, vortex-assisted extraction time, and absorbent amount, and the chromatographic conditions were investigated for the analysis of UA and OA in guava leaves. Under the optimized conditions, the method showed good linearity over a range of 1–320 μg mL−1, with correlation coefficients above 0.999. The limits of detection of UA and OA were 0.18 and 0.36 μg mL−1, respectively. The intraday and interday precision were below 1.95 and 2.55%, respectively. The accuracies of the UA and OA determinations ranged from 97.4 to 111.4%. The contents of UA and OA in the guava leaf samples were 2.50 and 0.73 mg g−1, respectively. These results demonstrate that the developed method is applicable to the simultaneous determination of UA and OA in guava leaves.

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