Research biologically active substances Atragene speciose Weinm.

2002 ◽  
Vol 1 (2) ◽  
pp. 55-59
Author(s):  
I. V. Shilova

From the overground part of Atragene speciosa Weinm. family Ranunculaceae, we obtained a fraction possessing a marked nootropic effect from which 3,4-dihydroxyphenylethanol-2, its 2-O-β-D-glucopyranoside, and a sum of glycosides of dihydroxybutane acid were isolated. In addition to the foregoing substances, in the pharmacologically active fraction we revealed the presence of triterpene glycosides (whose sapogenins are hederagenine and oleanolic acid), aminoacids and microelements. For the frist time the obtained data about the biologically active substances and their pharmacological activity give us the possibility of development of a new phytopreparation with the nootropic effect on the basis of Atragene speciose Weinm.

Fitoterapia ◽  
2020 ◽  
Vol 3 (3) ◽  
pp. 40-44
Author(s):  
Ye. Bazavluk ◽  
◽  
R. Vanko ◽  
Yu. Konechnyi ◽  
R. Shykula ◽  
...  

Keywords: Phlomis pungens, silver nanoparticles, spray, antibacterial activity. In the article the results of the study on Phlomis pungens and extracts of the plant are presented. The optimum conditions for obtaining the extracts were selected, the content of the biologically active substances (phenols and flavonoids) in the extracts was studied. The pharmacological activity of extracts of the plant and silver nanoparticles were examined. A new antiseptic in the form of spray based on Phlomis pungens extract and silver nanoparticles has been developed and studied.


2020 ◽  
Vol 50 (4) ◽  
pp. 707-716
Author(s):  
Valery Bogdanov ◽  
Andrei Simdiankin ◽  
Anna Pankina ◽  
Vadim Mostovoi

Introduction. Nutrition has a significant impact on human health, performance, well-being, and lifespan. The research objective was to develop formulations for functional compositions of seafood cryoconcentrates. Study objects and methods. The study featured concentrates obtained by cryotechnology from carcasses of Pacific squid (Todarodes pacificus), muscular shell of cucumaria (Cucumaria japonica), scallop mantle (Mizuhopecten yessoensis), octopus skin (Octopus dofleini), milt of Pacific herring (Pacific herring), and Japanese kelp (Laminaria japonica), as well as their multicomponent mixes. The content of biologically active substances was determined by the spectrophotometric method. The safety and biological value were biotested on Tetrahymena pyriformis. Results and discussion. The dry seafood concentrates had a high content of protein, minerals, and biologically active components, i.e. hexosamines, triterpene glycosides, carotenoids, nucleic acids, etc. The seafood cryoconcentrate mixes were divided into fortifying, restoring, immunomodulating, and mineral. Their chemical composition varied greatly, depending on the functional aim of the mix. For instance, the protein content varied from 28.8 to 57.6%, lipids – from 3.1 to 6.9%, carbohydrates – from 15.0 to 35.9%, minerals – from 10.9 to 22.9%, and hexosamines – from 2.2 to 5.6%. The experiments made it possible to substantiate the composition of the dry mixes. The compilation principle was based on the chemical profile of the raw material, as well as on the choice of biologically active substances. All the dry seafood concentrates showed a high relative biological value. However, the values differed, depending on the type of raw material: from 65.5% for the scallop mantle sample to 86.3% for the octopus skin sample. The difference could be explained by the chemical composition of the cryopowders and their availability to ciliate enzymes. Conclusion. The research resulted in formulations and functional mixes of dry seafood concentrates obtained by cryotechnology. The dry seafood concentrates can be used both independently and as functional compositions. The developed mixes demonstrated rather high content of animal proteins, minerals, and biologically active substance, e.g. hexosamines, triterpene glycosides, carotenoids, nucleic acids, etc. Their functional compositions proved to have excellent sensory properties; they are biologically safe for human consumption and have a high relative biological value.


Pharmacia ◽  
2021 ◽  
Vol 68 (4) ◽  
pp. 731-739
Author(s):  
Ani Slavik Dadayan ◽  
Lala Ashot Stepanyan ◽  
Tatevik Hovhannes Sargsyan ◽  
Anahit Mkhitar Hovhannisyan ◽  
Slavik Arshak Dadayan

Biologically active substances, the antioxidant and antimicrobial activity of aqueous, 70% ethylate and ethyl acetate extracts of fruits of maclura orange (Osage Orange), a representative of the Moraceae genus growing in the Ararat region of Armenia have been studied. It is found that plant extracts are rich in a wide range of pharmacologically active substances, in particular, tannins, flavonoids, organic acids, vitamins, anthocyanins, micro- and microelements, etc. Based on the investigations performed, extracts of maclura orange can be recommended as a source of biologically active substances (BAS), as well as a preventive and corrective agent in deficiency or imbalance of macro- and microelements in tissue and cellular structures in various pathological processes in the body. An ethanol extract of maclura fruits exhibits the antimicrobial activity against Bacillus subtilis 1820, E. Coli 5002, Serratia marcescens 5251 and Staphylococcus aureus ATCC-6538 strains. Maclura extracts can be used as an environmentally friendly source of antioxidants against early aging.


Author(s):  
S.S. Bekkuzhina ◽  
◽  
A.O. Rakhimzhanova ◽  
A.K. Talkanbayeva ◽  
S.A. Manabayeva ◽  
...  

Cistanche (C. deserticola) - is one of the valuable technical plants of the Kazakhstan flora. The value of cistanche is due to the high content of various polysaccharides, iridoids, and other biologically active substances in the stolons, which are widely used in the eastern countries as feedstock for the production of many pharmacologically active compounds with a wide spectrum of action: increasing tone, potency, antioxidant activity. However, to date, the natural reserves of cistanche are substantially depleted, which makes both the problem of preserving this species and the search for alternative sources of its biologically active substances urgent. It is known that the cell culture of higher plants is an alternative way to obtain rare plant biomass. This paper presents the results of work on optimizing methods for obtaining callus cell cultures from C. deserticola seeds. Several lines of callus cultures of cistanche cells were obtained, growth characteristics were studied. It was established that the proposed method for obtaining callus cultures of cistanche cells is quite effective, the obtained lines have good growth characteristics and differ in color, which is evidence of the synthesis of various secondary metabolites.


2020 ◽  
Vol 62 (1-2) ◽  
pp. 49-68
Author(s):  
T. O. Kondratiuk ◽  
T. V. Beregova ◽  
I. Yu. Parnikoza ◽  
S. Y. Kondratyuk ◽  
A. Thell

The identification of the diversity of microscopic fungi of lithobiont communities of the Argentine Islands in specimens collected during the 22nd Ukrainian Antarctic Expedition was the purpose of this work. Samples of rock, soil, mosses and lichens of rock micro-habitats of “Crustose lichen sub-formation and fruticose lichen and moss cushion sub-formation” were used in the work. These samples were used for extracting and cultivation of filamentous fungi on dense nutrient media. Determination of physiological and biochemical characteristics and identification of yeast-like fungi were performed using a microbiological analyser ‘Vitek-2’ (‘Bio Merieux’, France). Cultivation of microorganisms was carried out at temperatures from +2 to +37 °C. In results cultures of microscopic fungi of Zygomycota (Mucor circinelloides), Ascomycota (species of the genera cf. Tlielebolus, Talaromyces), representatives of the Anamorphic fungi group (Geomyces pannorum, species of the genera Alternaria, Acremonium, Aspergillus, Penicillium, and Cladosporium) were isolated from Antarctic samples. Microscopic fungi Penicillium spp. were dominated after the frequency in the studied samples (54.5%). Rhodotorula rubra and Candida sp. among isolated yeast fungi, and dark pigmented fungi represented by Aureobasidium pulhdans and Exophiala spp. were identified. The biological properties of a number of isolated fungi (the potential ability to synthesise important biologically active substances: melanins, carotenoids, lipids) are characterised. Mycobiota of rock communities of Argentine Islands is rich on filamentous and yeast fungi similarly to other regions of Antarctica. A number of fungi investigated are potentially able to synthesise biologically active substances. The dark pigmented species of the genera Cladosporium, Exophiala, Aureobasidium pulhdans, capable of melanin synthesis; ‘red’ yeast Rhodotorula rubra (carotenoid producers and resistant to toxic metals); Mucor circinelloides and Geomyces pannorum, lipid producers, are among these fungi. Yeast-like fungi assimilated a wide range of carbohydrates, which will allow them to be further used for cultivation in laboratory and process conditions. The collection of technologically promising strains of microorganisms, part of the Culture Collection of Fungi at Taras Shevchenko National University of Kyiv (Ukraine), is updated with isolated species (strains) of filamentous fungi and yeast – potential producers of biologically active substances, obtained within this study.


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