scholarly journals Actual aspects of standardization of medicinal vegetative raw materials containing flavonoids

2011 ◽  
Vol 10 (5) ◽  
pp. 150-154
Author(s):  
A. V. Kurkina
Keyword(s):  
Medicinal Plants ◽  
High Performance ◽  
Raw Materials ◽  
Mentha Piperita ◽  
Standard Samples ◽  
Tanacetum Vulgare ◽  
New Approaches ◽  
Plant Drugs ◽  
Aerva Lanata ◽  
Layer Chromatography

The main purpose of our paper is the studies on the substantiation of the new approaches to the chemical standardization of plant drugs and their medicines containing flavonoids. On the basis of the study of chemical composition of medicinal plants there were developed the methodic and methodological approaches to the standardization of plant drugs and preparations containing flavonoids. We studied such medicinal plants as Helichrysum arenarium (L.) Moench., Tanacetum vulgare L., Artemisia dracunculus L., Mentha piperita L., Agrimonia eupatoria L., Aerva lanata L., Matricaria chamomilla L. We used such methods as spectrophotometry, thin layer chromatography, high performance liquid chromatography. For purpose of standardization were recommended the state standard samples of rutin, cynaroside and isosalipurposide. The developed new approaches to standardization of medicinal plants containing flavonoids and their medicines will be useful in elaboration of new phytopharmaceuticals.

scholarly journals DETERMINATION OF SIMPLE SUGARS IN MEDICINAL PLANT MATERIALS BY THE METHOD OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY (FOR EXAMPLE, THE FRUITS OF SEA BUCKTHORN L. AND NETTLE LEAVES L.)

2020 ◽  
pp. 215-222
Author(s):  
Ol'ga Valer'yevna Trineeva ◽  
Aleksey Ivanovich Slivkin
Keyword(s):  
Medicinal Plant ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Raw Materials ◽  
Sea Buckthorn ◽  
Standard Samples ◽  
Plant Materials ◽  
Layer Chromatography

An analysis of the literature over the past 20 years has shown that when controlling the quality of drugs containing monosaccharides, as well as studying the composition of simple sugars in polysaccharide complexes of medicinal plants and not only, preference is given to physicochemical methods, as the most express, sensitive and informative. No means have been found in the scientific literature to identify and quantify simultaneously various monosaccharides by high performance thin layer chromatography (HPTLC). An economical and rapid method has been developed for the identification and quantitative determination of simple reducing sugars (by the example of glucose, rhamnose and xylose) by the HPTLC method. The optimal conditions for their chromatography in a thin layer of sorbent with a quantitative interpretation of HPTLC data on a personal computer were experimentally selected and theoretically substantiated. In a detailed study of the influence of the polarity of the system on the value of Rf, the intervals of values of the polarity of the eluent were chosen, in which these dependences become linear. Using the proposed dependencies, you can select different systems for the separation of monosaccharides in a thin layer of sorbent, so that the value of Rf fit into the optimal values. The proposed method was tested on medicinal plant raw materials of nettle dioica and sea buckthorn fruits of various conservation methods. Zones of simple sugars of characteristic color were found on the chromatograms of extracts from the studied raw materials, among which glucose, xylose and rhamnose were identified by the characteristic value of Rf values in comparison with reliable standard samples. The technique can be used in quality control of substances, single-component and complex preparations, plant objects, dietary supplements, premixes and products of the food industry.

High Performance Thin Layer Chromatography (HPTLC) for the Investigation of Medicinal Plants

2020 ◽  
Vol 16 ◽  
Author(s):  
Alper Gökbulut
Keyword(s):  
Medicinal Plants ◽  
High Performance ◽  
Separation Efficiency ◽  
Rapid Development ◽  
Herbal Remedies ◽  
Qualitative And Quantitative ◽  
Powerful Analytical Tool ◽  
Quantitative Examination ◽  
Data Acquisition And Processing ◽  
Layer Chromatography

Background: Chromatographic techniques such as TLC basically and, HPLC, GC, HPTLC equipped with various detectors are most frequently used for the qualitative and quantitative examination of herbals. Method: An overview of the recent literature concerning the usage of HPTLC for the analysis of medicinal plants has been reviewed. Results: During the last decade/s, HPTLC, a modern, sophisticated and automatized TLC technique with better and advanced separation efficiency, detection limit, data acquisition and processing, has been used for the analysis of herbal materials and preparations since the rapid development of technology in chromatography world. HPTLC with various detectors is a powerful analytical tool especially for the phytochemical applications such as herbal drug quantification and fingerprint analysis. Conclusion: In this review, a latest perspective has been established and some of the previous studies were summarized for the usage of HPTLC in the analysis of herbal remedies, dietary supplements and nutraceuticals.

scholarly journals Scientific-methodological approach for standardization of herbal raw materials and herbal preparations using high-performance thin-layer chromatography procedure

2021 ◽  
pp. 76-89
Author(s):  
К. О. Хохлова
Keyword(s):  
Quality Control ◽  
Thin Layer ◽  
Related Species ◽  
High Performance ◽  
Raw Materials ◽  
Methodological Approach ◽  
Herbal Drugs ◽  
Closely Related Species ◽  
Layer Chromatography

The high-performance thin-layer chromatography (HPTLC) is widely used for quality control of herbal raw materials (HRM). The purpose of this work were to define the problematic issues of quality control of herbal drugs in Ukraine and to develop a systematic scientific and methodological approach for the standardization of HRM and herbal drugs using the modern analytical technique of HPTLC: to optimize the existing and develop new methods of identification and determination of adulterations, and to develop alternative methods of quantitative determination, as well as studying of the stability. As a result of a critical evaluation of existing approaches for the standardization of HRM and herbal drugs of Ukraine’s flora, numerous problematic issues were identified, including the existence of a limited number of national monographs / national parts of monographs on the HRM of the Ukraine’s flora, which were not previously standardized by other pharmacopeias; non-consideration of Ukraine’s flora species (non-pharmacopoeial) in monographs on closely related species that introduced in the State Pharmacopoeia of Ukraine; lack of specificity of combinations of physical and chemical methods used for quality control; lack of specificity and reproducibility, the labor intensity of methods; necessity of usage of toxic solvents and precursors; use of different groups of markers and methods for the HRM and its finished product or HRM and its closely related species, etc. The proposed systematic scientific and methodological approach includes: a preliminary collection of information about the object of study, markers/groups of bioactive substances, approaches to its standardization; theoretical evaluation of existing techniques of quality control in monographs on HRM and experimental approbation using samples of domestic plants, determination of the need for optimization of existed methods by the parallel development of specific, reproducible and documented methods for identification, adulterations’ detection, quantification, and bio-detection (if necessary) by HPTLC method. The approach is illustrated with examples. The proposed approach can be used for comprehensive HPTLC analysis of domestic HRM and herbal drugs for research, prevention of adulteration, standardization of initial HRM and its finished product, pharmaceutical development, and stability studies.

scholarly journals Recent Trends in the Application of Chromatographic Techniques in the Analysis of Luteolin and Its Derivatives

Biomolecules ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 731 ◽  
Author(s):  
Juszczak ◽  
Zovko-Končić ◽  
Tomczyk
Keyword(s):  
Liquid Chromatography ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Analytical Methods ◽  
High Performance ◽  
Biological Effects ◽  
Immunomodulatory Activity ◽  
Layer Chromatography

Luteolin is a flavonoid often found in various medicinal plants that exhibits multiple biological effects such as antioxidant, anti-inflammatory and immunomodulatory activity. Commercially available medicinal plants and their preparations containing luteolin are often used in the treatment of hypertension, inflammatory diseases, and even cancer. However, to establish the quality of such preparations, appropriate analytical methods should be used. Therefore, the present paper provides the first comprehensive review of the current analytical methods that were developed and validated for the quantitative determination of luteolin and its C- and O-derivatives including orientin, isoorientin, luteolin 7-O-glucoside and others. It provides a systematic overview of chromatographic analytical techniques including thin layer chromatography (TLC), high performance thin layer chromatography (HPTLC), liquid chromatography (LC), high performance liquid chromatography (HPLC), gas chromatography (GC) and counter-current chromatography (CCC), as well as the conditions used in the determination of luteolin and its derivatives in plant material.

A preliminary study of multi-mycotoxins contamination in some selected South Africa medicinal plants

2020 ◽  
pp. 426
Author(s):  
Oluwaseun Mary Areo ◽  
Judith Zanle Phoku ◽  
Sefater Gbashi ◽  
Patrick Berka Njobeh
Keyword(s):  
Medicinal Plants ◽  
South African ◽  
High Performance ◽  
Raw Materials ◽  
High Performance Liquid Chromatographic ◽  
Hazardous Substances ◽  
Control Programmes ◽  
Pharmaceutical Industries ◽  
Preliminary Study ◽  
Liquid Chromatographic

The use of medicinal plants in folklore remedies and as sources of raw materials for pharmaceutical industries is extensively increasing. The problem surrounding the use of such plants rests with the manner in which such plants like other agricultural commodities are contaminated with fungi, some of which are toxigenic, with possible production of mycotoxins in such plants. This study was aimed at investigating the degree of mycotoxin contamination of 36 South African medicinal plants. A multi-mycotoxin extraction method was followed and mycotoxins so extracted were quantified by high performance liquid chromatography (HPLC). High performance liquid chromatographic data revealed the presence of aflatoxins (AFs), ochratoxin A (OTA) and zearalenone (ZEA) 0.03 to 31.46 µg/kg, 0.2 to 10.09 µg/kg and 0.1 to 23.35 µg/kg, respectively. Most of the plants were found to be contaminated with one or two mycotoxins tested for. The use of such contaminated medicinal plants may lead to high risk of mycotoxins consumption which might result to adverse human health problems and therefore represents a special hazard. In view of this, it is crucial to establish and implement fungal and mycotoxin control programmes so as to limit quality loss and exposure of consumers of these products to these hazardous substances that could be accompanied by ill-health.

Sign in / Sign up

Export Citation Format

Share Document