High Performance Thin Layer Chromatography (HPTLC) for the Investigation of Medicinal Plants

2020 ◽  
Vol 16 ◽  
Author(s):  
Alper Gökbulut
Keyword(s):  
Medicinal Plants ◽  
High Performance ◽  
Separation Efficiency ◽  
Rapid Development ◽  
Herbal Remedies ◽  
Qualitative And Quantitative ◽  
Powerful Analytical Tool ◽  
Quantitative Examination ◽  
Data Acquisition And Processing ◽  
Layer Chromatography

Background: Chromatographic techniques such as TLC basically and, HPLC, GC, HPTLC equipped with various detectors are most frequently used for the qualitative and quantitative examination of herbals. Method: An overview of the recent literature concerning the usage of HPTLC for the analysis of medicinal plants has been reviewed. Results: During the last decade/s, HPTLC, a modern, sophisticated and automatized TLC technique with better and advanced separation efficiency, detection limit, data acquisition and processing, has been used for the analysis of herbal materials and preparations since the rapid development of technology in chromatography world. HPTLC with various detectors is a powerful analytical tool especially for the phytochemical applications such as herbal drug quantification and fingerprint analysis. Conclusion: In this review, a latest perspective has been established and some of the previous studies were summarized for the usage of HPTLC in the analysis of herbal remedies, dietary supplements and nutraceuticals.

scholarly journals Actual aspects of standardization of medicinal vegetative raw materials containing flavonoids

2011 ◽  
Vol 10 (5) ◽  
pp. 150-154
Author(s):  
A. V. Kurkina
Keyword(s):  
Medicinal Plants ◽  
High Performance ◽  
Raw Materials ◽  
Mentha Piperita ◽  
Standard Samples ◽  
Tanacetum Vulgare ◽  
New Approaches ◽  
Plant Drugs ◽  
Aerva Lanata ◽  
Layer Chromatography

The main purpose of our paper is the studies on the substantiation of the new approaches to the chemical standardization of plant drugs and their medicines containing flavonoids. On the basis of the study of chemical composition of medicinal plants there were developed the methodic and methodological approaches to the standardization of plant drugs and preparations containing flavonoids. We studied such medicinal plants as Helichrysum arenarium (L.) Moench., Tanacetum vulgare L., Artemisia dracunculus L., Mentha piperita L., Agrimonia eupatoria L., Aerva lanata L., Matricaria chamomilla L. We used such methods as spectrophotometry, thin layer chromatography, high performance liquid chromatography. For purpose of standardization were recommended the state standard samples of rutin, cynaroside and isosalipurposide. The developed new approaches to standardization of medicinal plants containing flavonoids and their medicines will be useful in elaboration of new phytopharmaceuticals.

scholarly journals Recent Trends in the Application of Chromatographic Techniques in the Analysis of Luteolin and Its Derivatives

Biomolecules ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 731 ◽  
Author(s):  
Juszczak ◽  
Zovko-Končić ◽  
Tomczyk
Keyword(s):  
Liquid Chromatography ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Analytical Methods ◽  
High Performance ◽  
Biological Effects ◽  
Immunomodulatory Activity ◽  
Layer Chromatography

Luteolin is a flavonoid often found in various medicinal plants that exhibits multiple biological effects such as antioxidant, anti-inflammatory and immunomodulatory activity. Commercially available medicinal plants and their preparations containing luteolin are often used in the treatment of hypertension, inflammatory diseases, and even cancer. However, to establish the quality of such preparations, appropriate analytical methods should be used. Therefore, the present paper provides the first comprehensive review of the current analytical methods that were developed and validated for the quantitative determination of luteolin and its C- and O-derivatives including orientin, isoorientin, luteolin 7-O-glucoside and others. It provides a systematic overview of chromatographic analytical techniques including thin layer chromatography (TLC), high performance thin layer chromatography (HPTLC), liquid chromatography (LC), high performance liquid chromatography (HPLC), gas chromatography (GC) and counter-current chromatography (CCC), as well as the conditions used in the determination of luteolin and its derivatives in plant material.

High Performance Thin Layer Chromatography: A Mini Review

2016 ◽  
Vol 2 (4) ◽  
pp. 219-226
Author(s):  
S. V. Saibaba ◽  
P. Shanmuga Pandiyan
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Separation Efficiency ◽  
Analytical Tool ◽  
Combinatorial Approach ◽  
Herbal Drug ◽  
Biomedical Analysis ◽  
Layer Chromatography

High Performance Thin layer Chromatography (HPTLC) technique is a sophisticated and automated form of the thin-layer chromatography (TLC) with better and advanced separation efficiency and detection limits, and is often an excellent alternative to GC and HPLC. Applications of HPTLC include phytochemical and biomedical analysis, herbal drug quantification, active ingredient quantification, fingerprinting of formulations, and check for adulterants in the formulations. HPTLC is useful in detecting chemicals of forensic concern. Various advance techniques in reference to HPTLC like hyphenations in HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser have made HPTLC a power analytical tool in the field of analysis. Experts are of the opinion that HPTLC future to combinatorial approach and the utilization of instrumental HPTLC toward the analysis of drug formulations, bulk drugs, and natural products will increase in the future

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