Effects of a high-fat, high-carbohydrate diet on blood cells of rats

Aim. To study the effects of a high-fat, high-carbohydrate diet on erythrocytes and platelets of rats.Materials and methods. Male Wistar rats (n = 23) were used for the study. The rats were divided into a control group and an experimental group. The rats from the control group were fed with standard rat chow. The rats from the experimental group had received a high-fat and high-carbohydrate diet for 12 weeks. In the rats, body weight and blood pressure (BP) were measured, an oral glucose tolerance test was carried out, and hematological and lipid metabolism parameters were analyzed. The conductance of erythrocyte KCa-channels was measured by the potentiometric method, and platelet aggregation was determined by the turbidimetric method.Results. Feeding the rats with a high-fat, high-carbohydrate diet for 12 weeks resulted in obesity, BP elevation, hyperglycemia, impaired glucose tolerance, and dyslipidemia with pronounced triglyceridemia. In the experimental group, a rise in the number of leukocytes, mainly due to granulocytes, and an increase in the number of platelets and their collagen-induced aggregation were observed. The red blood cell count in the rats of the experimental group did not significantly differ from that of the control group. In the experimental group, multidirectional changes in the membrane potential were observed in response to the stimulation of the KCa-channels in the erythrocyte membrane with the Ca2+ ionophore A23187 or artificial redox systems.Conclusion. The obtained data indicate that a high-fat, high-carbohydrate diet leads to metabolic and hemorheological disorders that are typical of metabolic syndrome.

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High Carbohydrate Diet Increased Glucose Transporter Protein Levels in Jejunum but Did Not Lead to Enhanced Post-Exercise Skeletal Muscle Glycogen Recovery

Nutrients ◽

10.3390/nu13072140 ◽

2021 ◽

Vol 13 (7) ◽

pp. 2140

Author(s):

Yumiko Takahashi ◽

Yutaka Matsunaga ◽

Hiroki Yoshida ◽

Terunaga Shinya ◽

Ryo Sakaguchi ◽

...

Keyword(s):

Skeletal Muscle ◽

Glucose Transporter ◽

High Carbohydrate Diet ◽

Oral Glucose ◽

Carbohydrate Diet ◽

Post Exercise ◽

Glucose Administration ◽

Protein Levels ◽

High Carbohydrate ◽

Glucose Transporter 2

We examined the effect of dietary carbohydrate intake on post-exercise glycogen recovery. Male Institute of Cancer Research (ICR) mice were fed moderate-carbohydrate chow (MCHO, 50%cal from carbohydrate) or high-carbohydrate chow (HCHO, 70%cal from carbohydrate) for 10 days. They then ran on a treadmill at 25 m/min for 60 min and administered an oral glucose solution (1.5 mg/g body weight). Compared to the MCHO group, the HCHO group showed significantly higher sodium-D-glucose co-transporter 1 protein levels in the brush border membrane fraction (p = 0.003) and the glucose transporter 2 level in the mucosa of jejunum (p = 0.004). At 30 min after the post-exercise glucose administration, the skeletal muscle and liver glycogen levels were not significantly different between the two diet groups. The blood glucose concentration from the portal vein (which is the entry site of nutrients from the gastrointestinal tract) was not significantly different between the groups at 15 min after the post-exercise glucose administration. There was no difference in the total or phosphorylated states of proteins related to glucose uptake and glycogen synthesis in skeletal muscle. Although the high-carbohydrate diet significantly increased glucose transporters in the jejunum, this adaptation stimulated neither glycogen recovery nor glucose absorption after the ingestion of post-exercise glucose.

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Mixed lineage kinase 3 deficient mice are protected against the high fat high carbohydrate diet-induced steatohepatitis

Liver International ◽

10.1111/liv.12353 ◽

2013 ◽

Vol 34 (3) ◽

pp. 427-437 ◽

Cited By ~ 28

Author(s):

Samar H. Ibrahim ◽

Gregory J. Gores ◽

Petra Hirsova ◽

Michelle Kirby ◽

Lili Miles ◽

...

Keyword(s):

High Carbohydrate Diet ◽

High Fat ◽

Carbohydrate Diet ◽

Mixed Lineage Kinase ◽

High Carbohydrate ◽

Mixed Lineage Kinase 3 ◽

Deficient Mice

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Biochemical aspects of malabsorption in marasmus: effect of dietary rehabilitation

British Journal Of Nutrition ◽

10.1079/bjn19850143 ◽

1985 ◽

Vol 54 (3) ◽

pp. 567-575 ◽

Cited By ~ 3

Author(s):

H. C. Mehta ◽

A. S. Saini ◽

Harjit Singh ◽

P. S. Dhatt

Keyword(s):

Total Energy ◽

High Fat Diet ◽

High Protein Diet ◽

High Carbohydrate Diet ◽

High Protein ◽

Protein Diet ◽

Duodenal Juice ◽

High Fat ◽

Carbohydrate Diet ◽

High Carbohydrate

1. Sixty marasmic children were investigated for the absorption of xylose, proteins and fats. Their duodenal juice samples were also analysed for bile salts and microflora.2. The marasmic children were then studied in three groups of twenty by allocating them to three different dietary schedules: a high-protein diet (30% of the total energy from protein), a high-fat diet (40% of the total energy from fat) and a high-carbohydrate diet (70% of the total energy from carbohydrate) for 2 weeks and the previous measurements repeated.3. Whereas the high-fat diet resulted in improved fat absorption, along with an increase in total and conjugated bile acids, and the high-carbohydrate diet led to improved xylose absorption, the diet rich in protein resulted in an improvement in the absorption of all three dietary ingredients. It appears that a high-protein diet improves the overall absorption process by improving the intestinal environment as a whole, while high-carbohydrate and high-fat diets bring about adaptive changes related to the respective absorptive processes.

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