scholarly journals In vitro Efficacy of Bacillus spp. against Fusarium oxysporum f. sp. ciceri, the Causal Agent of Fusarium wilt of Chickpea

2017 ◽  
Vol 6 (11) ◽  
pp. 2751-2756 ◽  
Author(s):  
M. Karthick ◽  
C. Gopalakrishnan ◽  
E. Rajeswari ◽  
V. Karthik Pandi
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Causal Agent ◽  
Bacillus Spp

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

2019 ◽  
Vol 9 (2) ◽  
pp. 91
Author(s):  
Ghea Dotulong ◽  
Stella Umboh ◽  
Johanis Pelealu
Keyword(s):  
Solanum Tuberosum ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Leaf Extract ◽  
Solanum Tuberosum L ◽  
In Vitro Toxicity ◽  
Potato Plants ◽  
Colony Diameter ◽  
In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

scholarly journals Antagonisme Bacillus terhadap Infeksi Layu Fusarium pada Bibit Pisang Hasil Kultur Jaringan

2013 ◽  
Vol 15 (1) ◽  
pp. 21
Author(s):  
Hadiwiyono Hadiwiyono ◽  
Arief Widyantoro ◽  
Salim Widono
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
In Planta ◽  
Physiological Character ◽  
Important Disease

<p>Fusarium wilt (Fusarium oxysporum f.sp. cubense) is an important disease in banana. Fusarium wilt was hard to control because the pathogen can survive in many kind of soils type although there is no host. Therefore, overcoming the disease is urgently needed such as biological control. The endophytic Bacillus of banana was begun to use as antagonist agent to the pathogen. This research aimed to study the mechanism of antagonism and physiological character of Bacillus. There were 27 Bacillus isolates examined in-vitro to test the production of IAA, HCN, chitinase, pectinase, and antagonism. The top ten isolates based on the test in vitro were used to test in planta. The research showed that Bacillus were able to produce IAA, HCN, chitinase, pectinase, and able to retard the growth of Foc colony. The application of isolat B25 on banana seedling could decrease the disease intensity but still unable to prevent the fusarium wilt infection.</p>

scholarly journals Characterization and Pathogenicity of Fusarium oxysporum as the Causal Agent of Fusarium Wilt in Chili (Capsicum annuum L.)

2014 ◽  
Vol 8 (3) ◽  
pp. 121-126
Author(s):  
REJEKI SITI FERNIAH ◽  
◽  
BUDI SETIADI DARYONO ◽  
RINA SRI KASIAMDARI ◽  
ACHMADI PRIYATMOJO
Keyword(s):  
Fusarium Oxysporum ◽  
Capsicum Annuum ◽  
Fusarium Wilt ◽  
Causal Agent ◽  
Capsicum Annuum L

scholarly journals Action of Trichodermil 1306 in the control of Fusarium oxysporum f. sp. lycopersici in tomato crops

2020 ◽  
Vol 87 ◽  
Author(s):  
Isis Bruna Portolan ◽  
Silmara Rodrigues Pietrobelli ◽  
Gabriela Silva Moura ◽  
Aline Pomari Fernandes ◽  
Lisandro Tomas da Silva Bonome ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Tomato Seed ◽  
Trichoderma Spp ◽  
Tomato Crop ◽  
Tomato Seeds ◽  
Randomized Design ◽  
Lower Root ◽  
Completely Randomized Design

ABSTRACT Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici, is one of the most important diseases of tomato crop in Brazil. Several alternatives to fungicides have been evaluated in search for products that control this disease and have low environmental impact and toxicity to living beings. In this context, the use of Trichoderma spp. is an alternative to agrochemicals, since they are bioregulators and antagonists of several phytopathogens. This work aimed to evaluate the antagonistic and mycoparasitory action of the Trichodermil 1306 biofungicide against F. oxysporum, and its action in the control of fusarium wilt in tomato seeds and seedlings. The biofungicide was used at concentrations of 0, 50, 100, and 150% of the dose recommended for application of 20 L with a backpack sprayer. The experiment was conducted in a completely randomized design with five replications. Data were submitted to analysis of variance and mean compared by Tukey’s test at 5% probability. The 100% Trichodermil concentration was the most efficient for treating tomato seeds. Although this concentration did not show differences in relation to the lowest dose used (50%) in the tomato seed antagonism, mycoparasitism and F. oxysporum incidence tests were recommended because that the lowest concentration resulted in 18% lower root protrusion than that observed with 100% dosage. The in vitro antagonistic and mycoparasitory action of this biological fungicide indicates its potential in the control of fusarium wilt in tomato crops.

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