Action of Trichodermil 1306 in the control of Fusarium oxysporum f. sp. lycopersici in tomato crops

ABSTRACT Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici, is one of the most important diseases of tomato crop in Brazil. Several alternatives to fungicides have been evaluated in search for products that control this disease and have low environmental impact and toxicity to living beings. In this context, the use of Trichoderma spp. is an alternative to agrochemicals, since they are bioregulators and antagonists of several phytopathogens. This work aimed to evaluate the antagonistic and mycoparasitory action of the Trichodermil 1306 biofungicide against F. oxysporum, and its action in the control of fusarium wilt in tomato seeds and seedlings. The biofungicide was used at concentrations of 0, 50, 100, and 150% of the dose recommended for application of 20 L with a backpack sprayer. The experiment was conducted in a completely randomized design with five replications. Data were submitted to analysis of variance and mean compared by Tukey’s test at 5% probability. The 100% Trichodermil concentration was the most efficient for treating tomato seeds. Although this concentration did not show differences in relation to the lowest dose used (50%) in the tomato seed antagonism, mycoparasitism and F. oxysporum incidence tests were recommended because that the lowest concentration resulted in 18% lower root protrusion than that observed with 100% dosage. The in vitro antagonistic and mycoparasitory action of this biological fungicide indicates its potential in the control of fusarium wilt in tomato crops.

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Tingkat Ketahanan 70 aksesi Plasma Nutfah Kenaf terhadap Fusarium oxysporum Schletch; Assessment of Kenaf Germplasm Against Fusarium oxysporum Schletch

Buletin Tanaman Tembakau Serat & Minyak Industri ◽

10.21082/btsm.v8n2.2016.65-73 ◽

2017 ◽

Vol 8 (2) ◽

pp. 65

Author(s):

Supriyono Supriyono ◽

Titiek Yulianti

Keyword(s):

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Spore Suspension ◽

Randomized Design ◽

Resistant Accession ◽

New Varieties ◽

Completely Randomized Design ◽

Screen House ◽

Important Disease ◽

Research Institute

Salah satu penyakit penting yang sangat merugikan tanaman kenaf adalah penyakit layu Fusarium yang disebabkan oleh Fusarium oxysporum Schlecht. Tujuan penelitian ini adalah untuk mengevaluasi tingkat ketahanan aksesi kenaf terhadap jamur Fusarium oxysporum. Penelitian dilakukan di laboratorium dan rumah kasa Balai Penelitian Tanaman Pemanis dan Serat, Malang menggunakan rancangan acak lengkap) yang diulang tiga kali. Dalam evaluasi ini digunakan 70 aksesi dan 1 aksesi tahan (BG-52-135) yang digunakan sebagai kontrol. Inokulasi dilakukan pada 7 hari setelah tanam (HST) menggunakan suspensi spora dengan kerapatan105/ml sebanyak 100 ml setiap bak. Pengamatan intensitas serangan dilakukan mulai 10–40 hari setelah inokulasi (HSI) dengan interval pengamatan lima hari. Pengamatan persentase diskolorisasi batang dilakukan sekali pada 50 HSI. Hasil pengujian memperoleh 1 aksesi (FJ/017) sangat tahan dengan intensitas serangan terrendah (0,83%) dan 14 aksesi tahan dengan intensitas serangan <10%, 28 aksesi dengan ketahanan moderat, dan 27 aksesi yang rentan terhadap infeksi F. oxysporum. Aksesisi FJ/017 (aksesi yang sangat tahan) dan 14 aksesi yang tahan: 1064(SUC/012), 1061(SRB/082), 1035(FJ/005), 839(PARC/2709), 955(FJ/003), 842(PARC/2712), 1095(SUC/003), 838(PARC/2708), 957(FJ/ 007), 1065(SUC/023), 1042(CHN/056), 145(BL/118), 1036(FJ/006), dan 778(PARC/2466) dapat digunakan sebagai sumber ketahanan pada perakitan varietas baru. One of the important disease that very detrimental to kenaf is Fusarium wilt caused by Fusarium oxysporum Schlecht. The purpose of this study was to evaluate the response of 70 kenaf germplasms accessions against F. oxysporum. The study was conducted at the Phytopatology Laboratory and screen house of Indonesian Sweetener and Fiber Crops Research Institute, Malang using completely randomized design with three replicates. Seventy accessions and one resistant accession as control (1267 (BG-52-135) were used in this study. Inoculation of Fusarium was done 7 days after sowing (das) by sprinkling 100 ml of spore suspension into the soil. Observation of disease intensity started at 10–40 days after inoculation (dai) and repeated every five days. Percentage of stalk discolorization was estimated at 50 dai. The results showed that accession 1040 (FJ/017) had the lowest disease intensity (0.83%), hence was categorized as a highly resitant accession. Fourteen accessions were categorized as resistant with disease intensity below or equal to 10%; 28 accessions were moderate resistant; and 27 accessions were susceptible. FJ/017 (the highset resistant accession) and 14 resis-tant accessions (1064(SUC/012), 1061(SRB/082), 1035(FJ/005), 839(PARC/2709), 955(FJ/003), 842(PARC/ 2712), 1095(SUC/003), 838PARC/2708), 957(FJ/007), 1065(SUC/023), 1042(CHN/056), 145(BL/118), 1036 (FJ/006), dan 778(PARC/2466)) could be used as resistant genetic sources in developing new varieties.

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In Vitro Inhibition of Cellulolytic Enzymes of Fusarium Oxysporum by Trichoderma spp and Pseudomonas Fluorescens on Arachis Hypogaea L

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v3i1.12138 ◽

2015 ◽

Vol 3 (1) ◽

pp. 106-110

Author(s):

P. Rajeswari

Keyword(s):

Fusarium Oxysporum ◽

Pseudomonas Fluorescens ◽

Arachis Hypogaea ◽

Fusarium Wilt ◽

Trichoderma Viride ◽

Antagonistic Activity ◽

Cellulolytic Enzymes ◽

Trichoderma Spp ◽

In Vitro Inhibition

In an attempt to develop biocontrol system for management of Fusarium wilt in groundnut, Trichoderma viride, Trichoderma harzianum,and Pseudomonas fluorescens were evaluated for their antagonistic activity against Fusarium oxysporum in vitro. .Fusarium wilt diseasescaused by the fungus Fusarium oxysporum lead to significant yield losses of crops. Experiments were conducted on the effect of culture filtratesof T.viride (1%), T. harzianum (1.5%), and P. fluorescens (2%) on the in vitro inhibition of cellulolytic enzymes of Fusarium oxysporum. Theactivity of 1,4 endoglucanases, 1,4exoglucanase Cellobiase produced by Fusariumoxysporum was higher, when compared to control.Maximum inhibition of above Cellulolytic enzymes (1, 4 endoglucanases, 1,4exoglucanase, Cellobiase) was shown by T. viride treatment wasfollowed by T. harzianum and P. fluorescens. Of all the treatments, T. viride treatment showed higher rate of inhibition of Cellulolytic enzymesof Fusarium oxysporum followed by that of T. harzianum and P. fluorescens.This present study indicates that culture filtrate of T.viride(1%)is the best biocontrol agent in the inhibition of Fusarium oxysporum causing Fusarium wilt of Arachis hypogaea .LDOI: http://dx.doi.org/10.3126/ijasbt.v3i1.12138 Int J Appl Sci Biotechnol, Vol. 3(1): 106-110

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Aplikasi Beberapa Bentuk Formulasi Trichoderma spp dalam Mengendalikan Penyakit Layu Fusarium pada Tanaman Tomat

Jurnal Ilmiah Mahasiswa Pertanian ◽

10.17969/jimfp.v3i2.7478 ◽

2020 ◽

Vol 3 (2) ◽

pp. 20-30

Author(s):

Muhammad Fazil ◽

Tjut Chamzurni ◽

Rina Sriwati

Keyword(s):

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Liquid Product ◽

Wilt Disease ◽

Trichoderma Spp ◽

Soil Pathogens ◽

Tomato Plants ◽

Product Formulation ◽

Fusarium Wilt Disease ◽

Randomized Design

Abstrak: Fusarium oxysporum f.sp. lycopersici (Fol) adalah salah satu patogen tular tanah yang sangat berbahaya bagi tanaman tomat, hal itu dikarenakan patogen ini mampu bertahan dalam jangka waktu yang lama di dalam tanah. Salah satu alternatif pengendalian secara biologi yang ramah lingkungan adalah dengan cara memanfaatkan cendawan antagonis sebagai agen biokontrol yaitu Trichoderma spp. penggunaan cendawan antagonis sebagai agen hayati harus dalam bentuk formula yang tepat dengan bahan yang mudah tersedia. Penelitian ini bertujuan untuk mengetahui pengaruh aplikasi beberapa bentuk formulasi Trichoderma spp dalam mengendalikan penyakit layu fusarium pada tanaman tomat. Penelitian ini menggunakan Rancangan Acak Lengkap pola non faktorial yang terdiri dari 4 perlakuan dengan menggunakan 5 ulangan, setiap ulangan terdiri dari 4 unit tanaman. Susunan perlakuan bentuk formulasi berbahan aktif Trichoderma spp yaitu F0 = Kontrol (tanpa perlakuan),F1 = Formulasi pelet daun katuk 3 g (+ 10 butir)/polibag, F2 = Formulasi padat jagung kering 3 g/polibag dan F3 = Formulasi cair produk komersil 100 ml/polibag. Hasil penelitian menunjukkan bahwa aplikasi beberapa bentuk formulasi Trichoderma spp mampu memberikan pengaruh yang nyata terhadap persentase layu tanaman dan tinggi tanaman serta persentase batang yang xylemnya terdiskolorasi. Application of Several Forms by Trichoderma spp Formulation in Controlling Fusarium Wilt Disease on Tomato PlantsAbstract. Fusarium oxysporum f.sp. lycopersici (Fol) is one of the soil pathogens that is very harmful for tomato plants, it is because these pathogens are able to survive for a long time in the soil. One of the alternative biological controls that are environmentally friendly is by utilizing the antagonist fungus as a biocontrol agent that is Trichoderma spp. the use of antagonistic fungi as biological agents should be in the form of the right formula with easily available materials. This study aims to determine the effect of application of some form of Trichoderma spp formulation in controlling fusarium wilt disease in tomato plants. This research uses Completely Randomized Design of non factorial pattern consisting of 4 treatments using 5 replications, each replication consisting of 4 plant units. the active formulation by several form formulation of Trichoderma spp is F0 = Control (without treatment), F1 = Leaf pelet formulation 3 g (+ 10 grain) / polybag, F2 = Dry corn solid formula 3 g / polybag and F3 = Commercial liquid product formulation 100 ml / polybag. The results showed that the application of some form of formulation Trichoderma spp able to give a real effect on the percentage of wilting plants and plant height as well as the percentage of stems that discoloration on xylem.

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PENGARUH INOKULASI MIKORIZA VESIKULA ARBUSKULA (MVA) CAMPURAN TERHADAP KEMUNCULAN PENYAKIT LAYU FUSARIUM PADA TANAMAN TOMAT (Solanum lycopersicum)

Scripta Biologica ◽

10.20884/1.sb.2017.4.1.382 ◽

2017 ◽

Vol 4 (1) ◽

pp. 31

Author(s):

Uswatun Hasanah ◽

Purnomowati Purnomowati ◽

Uki Dwiputranto

Keyword(s):

Fusarium Oxysporum ◽

Incubation Period ◽

Fusarium Wilt ◽

Main Parameter ◽

Arbuscular Mycorrhizae ◽

Experimental Treatment ◽

Alternative Control ◽

Tomato Plants ◽

Randomized Design ◽

Completely Randomized Design

Tomato has an important role to fullfil the nutrition of society. The most important problem in the cultivation of tomatoes is fusarium wilt caused by Fusarium oxysporum it will attacking the plants from nursery to adult. One of the alternative control is use the Vesicles Arbuscular Mycorrhizae (VAM). The success of VAM infection in plants is determined by the dose and the inoculation. The aim of this research is to determine the effect of dose and mixture VAM inoculation to against the emergence of fusarium wilt in tomato plants and to determine the dosage mixture VAM inoculation as the most effective way for controlling fusarium wilt in tomato plants. The method of this research used experimental with completely randomized design. The experimental treatment consists of two types of treatment that are combined with used 5 doses of VAM mixture (0 g/plant, 10 g/plant, 12,5 g/plant, 15 g/plant, 17,5 g/plant) and used two ways of inoculation ( inoculation when the seed is planted and inoculation when transplanting the seeds). Each treatment was repeated 3 times and each test are three plants. The parameters was observed the incubation period of the disease and the intensity of fusarium wilt as the main parameter and the measurement of pH, temperature, humidity room, and the degree of infection as supporting parameters. The results of this research showed that the dosage and inoculation of VAM mixture is not able to reduce the emergence of fusarium wilt on tomatoes, but it was able to extend the incubation period of fusarium wilt on tomato plants a dose with 10 g /plant inoculated plants when the seeds are planted and inoculation when transplanting the seeds.

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Potensi jamur rizosfer bawang merah dalam menekan Fusarium oxysporum f.sp. cepae, penyebab penyakit busuk umbi bawang merah

Kultivasi ◽

10.24198/kultivasi.v19i1.22877 ◽

2020 ◽

Vol 19 (1) ◽

pp. 1015

Author(s):

Satriyo Restu Adhi ◽

Tarkus Suganda

Keyword(s):

Fusarium Oxysporum ◽

Rhizosphere Soil ◽

Control Methods ◽

Pests And Diseases ◽

Plant Pests ◽

Fungal Isolates ◽

Basal Rot ◽

Randomized Design ◽

Completely Randomized Design

Sari. Penyakit busuk umbi yang disebabkan oleh Fusarium oxysporum f.sp. cepae (Foc) merupakan salah satu penyakit penting pada bawang merah. Pengendalian penyakit busuk umbi yang ramah lingkungan adalah dengan menggunakan pengendalian biologis. Tujuan dari penelitian ini adalah untuk mendapatkan isolat jamur asal rizosfer tanaman bawang merah yang memiliki sifat antagonis terhadap Foc. Penelitian dilaksanakan di Laboratorium Fitopatologi Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran, pada bulan November 2017 hingga Januari 2018. Tahapan penelitian ini terdiri atas: (1) isolasi dari tanah rizosfer pertanaman bawang merah asal Desa Pelayangan Kabupaten Cirebon, (2) uji antagonisme secara in-vitro dengan menggunakan Rancangan Acak Lengkap (RAL), dan (3) uji kemampuan jamur rizosfer dalam memicu perkecambahan benih bawang merah. Dari hasil percobaan diperoleh 11 isolat jamur rizosfer yang terdiri atas genus Aspergillus, Penicillium, Paecilomyces, dan Trichoderma yang memiliki karakteristik mikroskopis yang berbeda satu sama lain. Hasil uji antagonisme menunjukkan bahwa 11 isolat jamur rizosfer yang diuji memiliki sifat antagonistik dan dapat menghambat jamur Foc secara in-vitro antara 65,58% hingga 84,71%. Isolat JRC1 (Aspergillus) dan JRC6 (Paecilomyces) memiliki sifat memicu perkecambahan benih bawang merah.Kata kunci: Bawang merah ∙ Jamur antagonis rizosfer ∙ Busuk umbi ∙ Fusarium oxysporum f.sp cepaeAbstract. Basal rot caused by Fusarium oxysporum f.sp. cepae (Foc) is one of the important diseases in shallot. Biological control is one of the environmentally friendly control methods. The purpose of this research was to obtain isolates of rhizospheric fungi of shallot which were antagonistic against Foc. Research has been conducted at the Laboratory of Phytopathology Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran, from November 2017 up to January 2018. The research consisted of: (1) isolation of fungi from shallot rhizosphere soil of shallot plantation located at Desa Pelayangan Cirebon, West Java, (2) in-vitro antagonistic test using a completely randomized design (CRD), and (3) test the ability of selected fungal isolates to triggering shallot seed germination. The experiment obtained 11 isolates of the antagonistic rhizospheric fungi consisted of Aspergillus, Penicillium, Paecilomyces, and Trichoderma which have different microscopic characteristics. The results showed that antagonistic rhizospheric fungi inhibit the growth of Foc. Their inhibitions rate ranged from 65.58% to 84.71%. The isolates of JRC1 (Aspergillus) and JRC6 (Paecilomyces) were able to trigger the germination of shallot seeds.Keywords: Shallot ∙ Antagonistic rhizospheric fungi ∙ Basal rot ∙ Fusarium oxysporum f.sp. cepae

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Invigorasi benih tomat kadaluarsa dengan ekstrak bawang merah pada berbagai konsentrasi dan lama perendaman

Jurnal Ilmiah Mahasiswa Pertanian ◽

10.17969/jimfp.v3i4.9392 ◽

2018 ◽

Vol 3 (4) ◽

pp. 175-184

Author(s):

Rahmat Rivai Lubis ◽

Trisda Kurniawan ◽

Zuyasna Zuyasna

Keyword(s):

Seedling Growth ◽

Significant Interaction ◽

Seed Viability ◽

Tomato Seed ◽

Onion Extract ◽

Tomato Seeds ◽

Randomized Design ◽

Completely Randomized Design ◽

Unit Unit ◽

Banda Aceh

Abstrak. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi dan lama perendaman ekstrak bawang merah serta interaksi keduanya terhadap viabilitas benih tomat kadaluarsa. Penelitian ini dilaksanakaan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi Fakultas Pertanian Universitas Syiah Kuala, Banda Aceh dari bulan April sampai Juni 2018. Unit-unit penelitian disusun berdasarkan Rancangan Acak Lengkap pola faktorial 5x3 dengan 3 ulangan dan dilanjutkan dengan uji Beda Nyata Jujur taraf 5% pada hasil uji F yang signifikan. Faktor pertama adalah konsentrasi ekstrak bawang merah dengan 5 taraf yaitu 0, 25, 50, 75 dan 100%, sedangkan faktor kedua adalah lama perendaman dengan 3 taraf yaitu 12, 24 dan 36 jam. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak bawang merah terbaik terdapat pada konsentrasi ekstrak bawang merah 25%. Lama perendaman tidak berpengaruh nyata terhadap tolok ukur pertumbuhan bibit. Tidak terjadi interaksi yang nyata antara konsentrasi ekstrak bawang merah dan lama perendaman terhadap tolok ukur pertumbuhan bibit.Invigoration of Expired Tomato Seeds with Onion Extract at Various Concentrations and Soaking DurationsAbstract. The purpose of this research was to investigate the effect of onion concentration and soaking duration and interaction between them on the expired tomato seed viability. The research was conducted at the Laboratory of Seed Science and Technology Department of Agrotechnology Faculty of Agriculture, Syiah Kuala University, Banda Aceh from April to June 2018. Treatment units were arranged according to Factorial Completely Randomized Design 5x3 in 3 replications, and the significant data than continued analized by Honestly Significant Different. The first factor was the concentrations of extract onion i.e 0, 25, 50, 75 and 100%, and the second factor was durations of soaking i.e 12, 24 and 36 hours. The result showed the best concentration for invigoration was 25% of onion extract. Soaking duration does not have a significant effect on seedling growth. There is no significant interaction between the concentration of onion extract and soaking duration on seedling growth.

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Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense

Semina Ciências Agrárias ◽

10.5433/1679-0359.2020v41n5supl1p2401 ◽

2020 ◽

pp. 2401-2410

Author(s):

Liliane Santana Luquine ◽

Luciano Ricardo Braga Pinheiro ◽

Saulo Alves Santos de Oliveira ◽

Harllen Sandro Alves Silva

Keyword(s):

Fusarium Oxysporum ◽

Biological Control Agent ◽

Control Agent ◽

In Vitro Tests ◽

Panama Disease ◽

Randomized Design ◽

Alternative Materials ◽

Completely Randomized Design ◽

Set Up

Detached plant organs are alternative materials to in vitro tests for selecting biocontrol agents. On the other hand, the use of scales to quantify injured areas can generate inconsistent results. Rhizospheric and endophytic bacteria were selected as growth inhibitors of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Panama disease of banana. For this, rhizome discs were treated with 200 µL of antagonist suspension (109 CFU mL?1) and inoculated with the pathogen. The material was placed in plastic gerbox boxes and incubated in BOD at 25 °C for 12 days. Afterward, the area of rhizome discs (mm2) colonized by Foc was quantified by digital images. The assay was set up in a completely randomized design, with four replications and three discs per replication. The control consisted of untreated and inoculated discs. The results showed the efficiency of this method in selecting the biological control agent, as the 26 isolates were group into five different clusters, with isolates belonging to four of these groups (from ‘1’ to ‘4’) being able to reduce Foc colonization. Isolates 520EB, 993EB, and 531EB had the highest potential for inhibition, with areas of 343.3, 344.1, 364.8 mm2, respectively, promoting inhibition ranging from 53 to 56 % of the colonized area compared to the control (782.6 mm2).

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Short Communication: Biological control of Fusarium wilt on banana plants using biofertilizers

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210540 ◽

2020 ◽

Vol 21 (5) ◽

Author(s):

Arief Widyantoro ◽

HADIWIYONO ◽

SUBAGIYA

Keyword(s):

Biological Control ◽

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Short Communication ◽

Wilt Disease ◽

In Planta ◽

Carrier Material ◽

Randomized Design ◽

Completely Randomized Design ◽

Important Disease

Abstract. Widyantoro A, Hadiwiyono, Subagiya. 2020. Biological control of Fusarium wilt on banana plants using biofertilizers. Biodiversitas 21: 2119-2123. Fusarium wilt is an important disease of banana caused by Fusarium oxysporum f. sp. cubense (FOC). The FOC is a weak parasite that attacks many bananas whose conditions are weak such as nutrients. Therefore, controlling Fusarium wilt through fertilization is important so that bananas are not nutrient deficient which can cause plants to be susceptible to FOC. The research aimed to study the effect of biofertilizer applications on FOC suppression in planta. Seven treatments were tested on banana seedlings cv. Ambon Kuning under a completely randomized design: (i) no biofertilizer, (ii) carrier material of biofertilizer, (iii) comparative biofertilizer product, (iv) Azotobacter, (v) Azospirillum, (vi) Streptomyces and (vii) Bacillus. The present study showed that biofertilizer agents were antagonistic to Fusarium wilt. The results showed that biofertilizer agents had the potential to suppress the Fusarium wilt in planta. Streptomyces and Bacillus were the most effective in controlling the Fusarium wilt. Azotobacter and Azospirillum had not been able to prevent the incidence of wilt disease.

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Efektivitas Rizobakteri Isolat Tanaman Tomat (Lycopersicum esculentum Mill.) terhadap Daya Hambat Pertumbuhan Koloni Patogen Fusarium oxysporum Tanaman Terung (Solanum melongena L.) secara in vitro.

Jurnal Ilmiah Mahasiswa Pertanian ◽

10.17969/jimfp.v3i4.9549 ◽

2018 ◽

Vol 3 (4) ◽

pp. 149-158

Author(s):

Rivayani Ahyamaqvirah ◽

Nanda Mayani ◽

Syamsuddin Syamsuddin ◽

Syamsuddin Syamsuddin

Keyword(s):

Fusarium Oxysporum ◽

High Activity ◽

Tomato Plant ◽

Solanum Melongena ◽

Lycopersicum Esculentum ◽

In Vitro Method ◽

Randomized Design ◽

Completely Randomized Design ◽

Banda Aceh

Abstrak. Penelitian ini bertujuan untuk mendapatkan rizobakteri isolat tanaman tomat yang mampu berperan sebagai agen biokontrol dalam menghambat pertumbuhan koloni patogen Fusarium oxysporum tanaman terung secara in vitro. Penelitian ini dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala, Darussalam Banda Aceh dari bulan Mei sampai Juli 2018. Rancangan yang digunakan pada uji antagonisme rizobakteri dengan patogen adalah Rancangan Acak Lengkap (RAL) non faktorial. Parameter yang diamati adalah persentase penghambatan pertumbuhan koloni patogen dan laju penghambatan pertumbuhan koloni patogen. Hasil penelitian menunjukkan persentase daya hambat rizobakteri yang tertinggi dalam menghambat pertumbuhan koloni patogen yaitu isolat SRK 5/3 dengan nilai 61,97% dengan aktivitas tinggi dan laju penghambatan isolat rizobakteri yang menekan pertumbuhan koloni, hifa, dan spora yaitu isolat SRK 5/3 dengan nilai 11,50 mm/hari.EFFECTIVENESS OF RIZOBACTERIAL TOMATO PLANT (Lycopersicum esculentum Mill.) ISOLATES ON PATHOGENIC COLUMN GROWTH POWER OF Fusarium oxysporum EGGPLANT (Solanum melongena L.) IN VITROAbstract. This study aims to obtain rhizobacteria from tomato isolates which are able to act as biocontrol agents for inhibiting the growth of pathogenic colonies of Fusarium oxysporum in eggplant plants by in vitro method. This research was carried out at the Laboratory of Seed Science and Technology, Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Darussalam Banda Aceh, from May to July 2018. The test of rhizobacterial antagonism with pathogens used non-factorial Completely Randomized Design (CRD). The parameters of this research were the percentage of inhibition of the pathogenic colonies growth and the rate of inhibition of the pathogenic colonies growth. The results showed that the highest percentage of rhizobacterial inhibition for hampering the growth of pathogenic colonies, SRK 5/3 isolates with 61.97% values with high activity and the rate of inhibition of rhizobacterial isolates which suppressed the growth of colony, hyphae, and spores, is 5/3 SRK isolates with the value of 11.50 mm / day.

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