scholarly journals Molecular Detection and Antimicrobial Susceptibility of Salmonella Species in Chickens

2021 ◽  
Vol 10 (9) ◽  
pp. 560-566
Author(s):  
Gloria Sugun Ehizibolo David ◽  
Ebenezer Bukola Ogundejii Kaikabo Ahmed ◽  
Agada O. Godwin Benshak Audu ◽  
Sugun Manasa
Keyword(s):  
Antimicrobial Susceptibility ◽  
Biochemical Analysis ◽  
Molecular Detection ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Selective Media ◽  
Antimicrobial Sensitivity ◽  
Cultural Properties ◽  
Plateau State ◽  
Antimicrobial Sensitivity Test

The study was intended for molecular detection of Salmonella Spp isolated from chicken. A total of 160 samples comprising of 40 liver, 40 spleen, 40 lungs and 40 intestines were collected directly into buffered peptone in universal bottles at the poultry slaughter houses of the four districts in Jos South Local Government area of Plateau State. The samples were enriched in 10 ml of Rappaport-Vassiliades broth and cultured onto Xylems Lysine Deoxycholate (XLD) agar for the isolation of bacteria. The isolated bacteria were identified by studying staining characteristics, cultural properties on different selective media, biochemical tests, catalase and coagulase test, and finally by PCR. Out of 160 samples, 65 (41%) samples were found positive for Salmonella Spp on XLD and 24 (37%) positive by biochemical analysis. Two(2) Salmonella isolates were amplified by 942 bp gene based PCR. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the Salmonella isolates were resistant to amoxycillin (100%) and erythromycin (100%), gentamicin (100%), Clindamycin (100%) streptomycin (100%), tetracycline (100%), sulphamethoxazole/trimethoprim (100%) but sensitive to Ceftiofur (100%).

scholarly journals MOLECULAR DETECTION AND CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM RAW MILK SOLD IN DIFFERENT MARKETS OF BANGLADESH

2017 ◽  
Vol 14 (2) ◽  
pp. 277-282 ◽  
Author(s):  
M. A. Islam ◽  
S. M. L. Kabir ◽  
M. T. Rahman
Keyword(s):  
Molecular Detection ◽  
Culture Media ◽  
Raw Milk ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Selective Media ◽  
Milk Samples ◽  
Antimicrobial Sensitivity ◽  
Antimicrobial Sensitivity Test

The study was intended for molecular detection of S. aureus isolated from raw cow’s milk. A total of 20 milk samples were collected from different upazila markets of Jamalpur, Tangail, Kishoreganj and Netrokona districts of Bangladesh. Milk samples were cultured onto various culture media for the isolation of bacteria. The isolated bacteria were identified by studying cultural properties on different selective media, biochemical tests, and finally by PCR. Out of 20 samples, 15 (75%) milk samples were found to be positive for S. aureus. S. aureus specific 16S rRNA gene was amplified from all isolates and identified as S. aureus. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the S. aureus isolates were resistant to amoxicillin (100%), erythromycin (73.33%) and tetracycline (73.33%) but sensitive to azithromycin (93.33%), ciprofloxacin (93.33%), gentamicin (100%), norfloxacin (86.67%) and streptomycin (86.67%).

scholarly journals Occurrence and antimicrobial susceptibility of Salmonella spp. isolated from domestic pigeons Columba livia var. domestica in 2007-2017 in Poland

2019 ◽  
Vol 75 (05) ◽  
pp. 6280-2019
Author(s):  
ALEKSANDRA LEDWOŃ ◽  
MAGDALENA RZEWUSKA ◽  
MICHAŁ CZOPOWICZ ◽  
MAGDALENA KIZERWETTER-ŚWIDA ◽  
DOROTA CHROBAK-CHMIEL ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Columba Livia ◽  
Diffusion Method ◽  
Carrier Status ◽  
Salmonella Spp ◽  
Internal Organs ◽  
Selective Media ◽  
Bacterial Diseases ◽  
Domestic Pigeons

Salmonellosis is one of the most important bacterial diseases in pigeons. The disease occurs in birds of different ages, and long-term Salmonella spp. carrier status is a major problem. The study included 499 samples from live pigeons and internal organs from 265 necropsied birds. The samples were cultured on a standard and selective media. Their antimicrobial susceptibility was carried out by the Kirby-Bauer diffusion method. Thirty one Salmonella spp. isolates were cultured. Most isolates were obtained from the internal organs of dead or euthanised pigeons, and only single isolates were obtained from droppings and cloacal swabs. A total of 4% of the samples tested were positive. The greatest percentage of isolates was susceptible to enrofloxacin (96.3%) and florfenicol (95.7%). Sulfamethoxazole and trimethoprim combination was effective against 73.9% of the isolates. Despite the fact of increasing antibiotic resistance of the investigated bacteria, it is comforting that enrofloxacin remains highly effective despite its widespread use in birds.

scholarly journals MOLECULAR DETECTION AND CHARACTERIZATION OF SALMONELLA SPP. ISOLATED FROM FRESH FISHES SOLD IN SELECTED UPAZILA MARKETS OF BANGLADESH

2017 ◽  
Vol 14 (2) ◽  
pp. 283-287 ◽  
Author(s):  
S. K. Seel ◽  
S. M. L. Kabir ◽  
M. A. Islam
Keyword(s):  
Antimicrobial Susceptibility ◽  
Molecular Detection ◽  
Antimicrobial Agents ◽  
Susceptibility Test ◽  
Aquatic Environments ◽  
Antimicrobial Susceptibility Test ◽  
Salmonella Spp ◽  
Resistance Patterns ◽  
Health Significance

Aquatic environments are the major reservoirs of Salmonella. Therefore, fishery products have been recognized as a major carrier of food-borne organism. Fish is known to harbor bacteria of public health significance. Aquatic environments are known to influence the bacterial loads in the harvested fish. The present work was undertaken for molecular detection and characterization of Salmonella species isolated from fresh fishes sold in different markets of Jamalpur,Tangail, Kishorganj and Netrokona districts of Bangladesh. The isolates were identified by their morphological, cultural and biochemical characteristics with standard reference organisms, and molecular methods. Out of 20 pangas fish (Pangasius spp.) samples the number of samples found to be positive for Salmonella spp was 14 (70%); of 20 koi fish (Anabas spp.) samples this number was 17 (85%); and of 20 tilapia fish (Oreochromis spp.) samples it was 15 (75%). All the isolates of Salmonella were confirmed by targeting genus specific histidine transport operon gene. Antimicrobial susceptibility test was performed to know the susceptibility and resistance patterns of the isolates to different antimicrobial agents. Results of antimicrobial susceptibility test shows that 40 (86.95%) isolates were found to be resistant to azithromycin, 42 (91.30%) were resistant to erythromycin. On the other hand all isolates were 100% susceptible to ciprofloxacin and gentamicin, 38 (82.62%) were susceptible to norfloxacin, 40 (86.95%) were susceptible to streptomycin.

scholarly journals Assessment of Antimicrobial Resistance of Salmonella spp. Isolated from Farmed Asian Clam Corbicula Fluminea

2020 ◽  
Vol 78 (2) ◽  
pp. 99-103
Author(s):  
Jing Jing Ngiam ◽  
Yusof Akrimah ◽  
Eh Rak Aweng ◽  
Seong Wei Lee
Keyword(s):  
Antibiotic Resistance ◽  
Corbicula Fluminea ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
Salmonella Spp ◽  
Asian Clam ◽  
Antimicrobial Sensitivity ◽  
Clam Corbicula ◽  
Mar Index ◽  
Antimicrobial Sensitivity Test

AbstractIn the present study, Salmonella spp. was successfully isolated from Asian clam Corbicula fluminea by using Xylose Lysine Deoxycholate (XLD) selective agar. A total of 200 bacterial colonies from live Asian clams were isolated and subjected to antimicrobial sensitivity test by using disc diffusion method. A total of 18 antibiotics was applied in the present study, namely oxolinic acid, nalidixic acid, erythromycin, tetracycline, doxycycline, oleandomycin, oxytetracycline, spiramycin, ampicillin, kanamycin, fosfomycin, florfenicol, lincomycin, novobiocin, chloramphenicol, amoxycillin, flumequine and sulphamethoxazole. The findings of the present study showed that total antibiotic sensitive case for Salmonella spp. isolated from C. fluminea sample was 50% or 1800 cases. This was followed by antibiotic resistance case 45% or 1620 cases and intermediary antibiotic sensitive case (5% or 180 cases). Based on the results of the present study, tetracycline, doxycycline, oxytetracycline and flumequine showed the highest inhibition of isolated Salmonella spp. The multiple antibiotic resistance (MAR) index was 0.36, indicating the sampled clams were highly exposed to the tested antibiotics.

scholarly journals Prevalence and antimicrobial susceptibility of salmonella species isolate from slaughtered cows in Iraq

2013 ◽  
Vol 37 (1) ◽  
pp. 96-101
Author(s):  
Afaf Abdulrahman Yousif
Keyword(s):  
Antimicrobial Susceptibility ◽  
Brilliant Green ◽  
Diagnostic Study ◽  
Mesenteric Lymph Nodes ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Antimicrobial Drugs ◽  
Antibiotic Susceptibility Pattern ◽  
Central Public Health ◽  
Mesenteric Lymph

This study was planned to determine the prevalence and antimicrobial susceptibility of Salmonella spp. One hundred bovine with different age and both sexes at slaughterhouse were examined. Four hundred samples were collected from different internal organ (bile, Liver, spleen and mesenteric lymph nodes). Diagnostic study depended upon the morphological and cultural properties of the isolates on some selective media like Brilliant green, XLD, SS agar which used in addition to different biochemical tests, API-20E )Enterobacteriaceae Identification Kit.). Isolates were serotyped at the Central Public Health Laboratories (National Center of Salmonellae in Baghdad, Iraq). Eleven isolates consisting of Salmonella anatum (9.09%), S.newport (27.27%), S.enteritidis (45.45 %) and S.ohio (18.18%). The percentage of infection were 3% in the mesenteric lymph node and liver, (4%) in bile, and (1%) in spleen. The antibiotic susceptibility pattern of Salmonella against 15 antimicrobial drugs revealed that all isolates were sensitive (100%) to ciprofloxacin and amicacin. And most of isolates were resistance to cloxacillin, cefixime, amoxicillin. and gave intermediate results to neomycin, gentamycin, tetracycline and streptomycin.

scholarly journals MOLECULAR DETECTION AND CHARACTERIZATION OF ESCHERICHIA COLI ISOLATED FROM RAW MILK SOLD IN DIFFERENT MARKETS OF BANGLADESH

2017 ◽  
Vol 14 (2) ◽  
pp. 271-275 ◽  
Author(s):  
M. A. Islam ◽  
S. M. L. Kabir ◽  
S. K. Seel
Keyword(s):  
Escherichia Coli ◽  
Molecular Detection ◽  
Culture Media ◽  
Raw Milk ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
E Coli ◽  
Milk Samples ◽  
Antimicrobial Sensitivity

     The study was intended for molecular detection of E. coli isolated from raw cow’s milk. A total of 20 milk samples were collected from different upazila markets of Jamalpur, Tangail, Kishoreganj and Netrokona districts of Bangladesh. Milk samples were cultured onto various culture media for the isolation of bacteria. The isolated bacteria were identified by studying staining characteristics, cultural properties on different selective media, biochemical tests, catalase and coagulase test, and finally by PCR. Out of 20 samples, 15 (75%) milk samples were found positive for E. coli. 15 Escherichia coli isolates were amplified by 16S rRNA gene based PCR. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the E. coli isolates were resistant to amoxycillin (86.67%) and erythromycin (73.33%) but sensitive to azithromycin (53.33%), ciprofloxacin (86.67%), gentamicin (86.67%), norfloxacin (80%) and streptomycin (66.67%).

scholarly journals Isolation, Identification, and Antimicrobial Susceptibility Test of Bacteria from Vulva Swab of African Pygmy Hedgehog (Atelerix albiventris) and Sunda Porcupine (Hystrix javanica)

2021 ◽  
Vol 33 ◽  
pp. 06009
Author(s):  
Anindya Dwi Ash-Santri ◽  
Vinsa Cantya Prakasita ◽  
Yosua Kristian Adi ◽  
Teguh Budipitojo ◽  
Agnesia Endang Tri Hastuti Wahyuni
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Proteus Mirabilis ◽  
Susceptibility Test ◽  
Penicillin G ◽  
Diffusion Method ◽  
Antimicrobial Susceptibility Test ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Selective Media

Atelerix albiventris and Hystrix javanica are currently traded as pets or consumed in Indonesia, but there has been no research about bacteria from the vulva swab before. This research aims to isolate and identify bacteria from the vulva swabs of Atelerix albiventris and Hystrix javanica, and identify their antibiotic susceptibility. Samples were isolated by blood agar plates and selective media and identified by biochemical tests. Kirby Bauer’s disk diffusion method was used for the antimicrobial susceptibility test. The result showed that from Atelerix albiventris was isolated and identified Escherichia coli and Proteus mirabilis, while from Hystrix javanica was isolated and identified Escherichia coli. The identified Escherichia coli was sensitive to Amikacin, Amoxycillin, Ampicillin, Enrofloxacin, Fosfomycin, Chloramphenicol, Tetracycline, Trimethoprim, and Kanamycin; intermediate to Streptomycin; and resistant to Erythromycin and Penicillin G. The identified Proteus mirabilis was sensitive to Amikacin, Amoxycillin, Kanamycin, Enrofloxacin, and Fosfomycin; and resistant to Erythromycin, Penicillin G, Streptomycin, Ampicillin, Chloramphenicol, Tetracycline, and Trimethoprim. This research concludes that Escherichia coli and Proteus mirabilis were isolated from vulva swab of Atelerix albiventris and Hystrix javanica, Escherichia coli was sensitive to eight antibiotics, while Proteus mirabilis was sensitive to five antibiotics.

scholarly journals Pathology of Fowl Paratyphoid and Molecular Detection of Its Pathogen in Layer Chickens

2021 ◽  
Vol 24 (2) ◽  
pp. 47-57
Author(s):  
J Alam ◽  
T Chakma ◽  
MS Islam ◽  
MT Islam ◽  
MAHNA ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Salmonella Enteritidis ◽  
Molecular Detection ◽  
Inflammatory Cells ◽  
Chain Reaction ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Gross Lesions ◽  
Pcr Method ◽  
Polymerase Chain

The study was aimed to ascertain the pathology of fowl paratyphoid and molecular detection of its causal agent (Salmonella spp) in chickens. Pathological and swab samples were collected from layers in Gazipur district, Bangladesh. For observing the gross and microscopic lesions of different organs necropsy and histopathology were done, and to isolate and identify the Salmonella spp, different bacteriological tests and Polymerase Chain Reaction (PCR) were performed. Swabs from 150 chickens showed 66% of salmonellosis. Gram’s staining of isolated bacteria showed pink colored rod shaped bacilli. In biochemical tests, Salmonella fermented dextrose, maltose, xylose, arabinose, dulcitol, mannitol except lactose and sucrose. Investigation of gross lesions at necropsy revealed hemorrhage and congestion in intestine, liver, spleen and ovaries. Necrotic foci were found in liver and spleen, and button like ulceration in cecal tonsils as well. Microscopic lesions included hemorrhage and focal necrosis in liver and spleen. Congestion and infiltrations of inflammatory cells were observed in small intestine. Ovary was hemorrhagic and there was infiltration of heterophils. Biochemically positive and isolated Salmonella organisms were confirmed by PCR method using invA and IE1 primers. The final results showed that a total of 91.7% Salmonella suspected cultures were confirmed as Salmonella Enteritidis. Ann. Bangladesh Agric. (2020) 24(2) : 47-57

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