Occurrence and antimicrobial susceptibility  of Salmonella spp. isolated from domestic pigeons  Columba livia var. domestica in 2007-2017 in Poland

AbstractOccurrence of Salmonella spp. in captive wild animal species in India is largely unknown. The purpose of this study was to determine the occurrence of different Salmonella serotypes, antimicrobial resistance patterns and genotypic relatedness of recovered isolates. A total of 370 samples including faecal (n = 314), feed and water (n = 26) and caretakers stool swabs (n = 30) were collected from 40 different wild animal species in captivity, their caretakers, feed and water in four zoological gardens and wildlife enclosures in India. Salmonellae were isolated using conventional culture methods and tested for antimicrobial susceptibility with the Kirby–Bauer disc diffusion method. Salmonella isolates were serotyped and genotyping was performed using enterobacterial repetitive intergenic consensus (ERIC) PCR and 16S rRNA sequencing. Animal faecal samples were also subjected to direct PCR assay. Salmonella was detected in 10 of 314 (3.1%) faecal samples by isolation and 18 of 314 (5.7%) samples by direct PCR assay; one of 26 (3.8%) feed and water samples and five of 30 (16.7%) caretakers stool swabs by isolation. Salmonella was more commonly isolated in faecal samples from golden pheasants (25%; 2/8) and leopard (10%; 2/20). Salmonella enterica serotypes of known public health significance including S. Typhimurium (37.5%; 6/14), S. Kentucky (28.5%; 4/14) and S. Enteritidis (14.3%; 2/14) were identified. While the majority of the Salmonella isolates were pan-susceptible to the commonly used antibiotics. Seven (43.7%; 7/16) of the isolates were resistant to at least one antibiotic and one isolate each among them exhibited penta and tetra multidrug-resistant types. Three S. Kentucky serotype were identified in a same golden pheasants cage, two from the birds and one from the feed. This serotype was also isolated from its caretaker. Similarly, one isolate each of S. Typhimurium were recovered from ostrich and its caretaker. These isolates were found to be clonally related suggesting that wildlife may serve as reservoir for infections to humans and vice versa. These results emphasise the transmission of Salmonella among hosts via environmental contamination of feces to workers, visitors and other wildlife.

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Incidence and antimicrobial susceptibility of Salmonella, Listeria, and Campylobacter spp. in raw “souvlaki” marketed in Thessaloniki (Northern Greece)

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.15859 ◽

2018 ◽

Vol 66 (3) ◽

pp. 161 ◽

Cited By ~ 1

Author(s):

N. SOULTOS (Ν. ΣΟΥΛΤΟΣ) ◽

E. IOSSIFIDOU (Ε. ΙΩΣΗΦΙΔΟΥ) ◽

E. PSOMAS (Ε. ΨΩΜΑΣ) ◽

Z. TZIKAS (Ζ. ΤΖΗΚΑΣ) ◽

T. LAZOU (Θ. ΛΑΖΟΥ)

Keyword(s):

Antimicrobial Susceptibility ◽

Thermal Processing ◽

Antimicrobial Agents ◽

Meat Product ◽

Diffusion Method ◽

Salmonella Spp ◽

Northern Greece ◽

Food Borne ◽

Food Borne Infections ◽

Campylobacter Spp

Souvlaki is a popular Greek meat product consisting of small chunks or cubes of pork threaded on a small wooden or metal skewer. In the present study, 105 samples of raw pork souvlaki obtained from retail shops in Thessaloniki (Northern Greece) were screened for the incidence of Salmonella, Listeria, and Campylobacter and their susceptibility to various antimicrobial agents; serotyping of the isolates was also performed. Of the samples tested, 1.9% were positive for Salmonella and yielded 3 serovars (S. SaintPaul, S. Fyris and S. Typhimurium); 31.4% proved positive for Listeria spp. with 6.7% yielding L. monocytogenes isolates belonged to molecular serogroups 2 (serotypes 1/2c and 3c) and 4 ( serotypes 4b, 4d, and 4e). Campylobacter spp. were not detected in any of the samples tested. The antimicrobial susceptibility to various antimicrobial agents of 11 Salmonella strains and 7 L. monocytogenes strains was also determined by disc diffusion method. Salmonella spp. were susceptible to a panel of 12 antibiotics but displayed intermediate resistance to tetracycline. L. monocytogenes isolates were resistant to nalidixic acid and ceftriaxone, partly resistant to clindamycin and cefotaxime, but sensitive to all antibiotics commonly used in veterinary and human listeriosis. Our findings indicate that souvlaki could be a potential vehicle of food borne infections due to strains of L. monocytogenes and Salmonella spp. in the case of inadequate thermal processing. In addition, good hygienic practices must be applied to avoid cross-contamination during preparation or handling of the product.

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Antimicrobial Susceptibility Pattern of Salmonella spp. Isolated from Enteric Fever Patients in Nepal

Infectious Disease Reports ◽

10.3390/idr13020037 ◽

2021 ◽

Vol 13 (2) ◽

pp. 388-400

Author(s):

Anu Maharjan ◽

Binod Dhungel ◽

Anup Bastola ◽

Upendra Thapa Shrestha ◽

Nabaraj Adhikari ◽

...

Keyword(s):

Drug Resistance ◽

Minimum Inhibitory Concentration ◽

Nalidixic Acid ◽

Antimicrobial Susceptibility ◽

Enteric Fever ◽

Inhibitory Concentration ◽

Diffusion Method ◽

Susceptibility Pattern ◽

Salmonella Spp ◽

Blood Samples

Introduction: Enteric fever, a systemic infection caused by Salmonella enterica Typhi and S. enterica Paratyphi is one of the most common infections in developing countries such as Nepal. Aside from irrational practices of antibiotic use, mutations in chromosomal genes encoding DNA gyrase and Topoisomerase IV and by plasmid mediated quinolone resistant (PMQR) genes are suggested mechanisms for the development of resistance to nalidixic acid and reduced susceptibility to ciprofloxacin. Regardless of high endemicity of enteric fever in Nepal, there is paucity of studies on prevalence and drug-resistance of the pathogen. Therefore, this study aimed to assess the antibiotic susceptibility pattern of Salmonella isolates and determine the minimum inhibitory concentration of ciprofloxacin. Methods: A total of 1298 blood samples were obtained from patients with suspected enteric fever, attending Sukraraj Tropical and Infectious Disease Hospital (STIDH) during March–August, 2019. Blood samples were inoculated immediately into BACTEC culture bottles and further processed for isolation and identification of Salmonella Typhi and S. Paratyphi. Axenic cultures of the isolates were further subjected to antimicrobial susceptibility testing (AST) by using the modified Kirby–Bauer disc diffusion method based on the guidelines by CLSI. The minimum inhibitory concentration (MIC) of ciprofloxacin was determined by agar-dilution method. Results: Out of 1298 blood cultures, 40 (3.1%) were positive for Salmonella spp. among which 29 (72.5%) isolates were S. Typhi and 11 (27.5%) isolates were S. Paratyphi A. In AST, 12.5% (5/40), 15% (6/40) and 20% (8/40) of the Salmonella isolates were susceptible to nalidixic acid, ofloxacin and levofloxacin, respectively, whereas none of the isolates were susceptible to ciprofloxacin. The MIC value for ciprofloxacin ranged from 0.06-16 µg/mL in which, respectively, 5% (2/40) and 52.5% (21/40) of the isolates were susceptible and resistant to ciprofloxacin. None of the isolates showed multidrug-resistance (MDR) in this study. Conclusion: This study showed high prevalence of quinolone-resistant Salmonella spp., while there was marked re-emergence of susceptibilities to traditional first option drugs. Hence, conventional first-line-drugs and third-generation cephalosporins may find potential usage as the empirical drugs for enteric fever. Although our reporting was free of MDR strains, extensive surveillance, augmentation of diagnostic facilities and treatment protocol aided by AST report are recommended for addressing the escalating drug-resistance in the country.

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Factors Associated with Salmonella Infection in Patients with Gastrointestinal Complaints Seeking Health Care at Regional Hospital in Southern Highland of Tanzania

10.21203/rs.2.12908/v4 ◽

2020 ◽

Author(s):

Fadhili Ashery Ngogo ◽

Agricola Joachim ◽

Ahmed M Abade ◽

Susan F Rumisha ◽

Mucho M Mizinduko ◽

...

Keyword(s):

Abdominal Pain ◽

Antimicrobial Susceptibility ◽

Public Health Problem ◽

Regional Hospital ◽

Diffusion Method ◽

Salmonella Infection ◽

Salmonella Spp ◽

Factors Associated ◽

Gastrointestinal Complaints ◽

Southern Highland

Abstract Background Salmonellosis remains an important public health problem globally. The disease is among the leading causes of morbidity and mortality in developing countries that experience poor hygiene and lack of access to clean and safe water. There was an increase in reported cases of Salmonellosis in Njombe region, Southern Highland of Tanzania between 2015 and 2016, based on clinical diagnosis. Nevertheless, little is known about the factors contributing to the transmission of this disease in the region. This study was conducted to determine the prevalence, antimicrobial susceptibility, and factors associated with Salmonella infection among patients who report gastrointestinal complaints. Methods A cross-sectional study was conducted from December 2017 to February 2018 among patients with gastrointestinal complaints at Kibena Regional Hospital. Stool samples were submitted for isolation of Salmonella spp. Identification was based on conventional biochemical tests and serotyping to differentiate typhoid and non-typhoid Salmonella (NTS). Antimicrobial susceptibility was performed using the Kirby-Bauer disc diffusion method. Multivariable logistic regression analysis was performed to examine the factors independently associated with Salmonella infection. Results The prevalence of Salmonella infection among participants with gastrointestinal complaints was 16.5% (95% CI: 12.7-21.1) of them, 83.7%, 95% CI: 70.9-91.5 were NTS while 16.3%, 95% CI: 8.5-29.0 were Typhoid Salmonella species. All isolates were sensitive to ceftriaxone and ciprofloxacin, whereas 27.8% and 100% were resistant to co-trimoxazole and ampicillin respectively. The odd of Salmonella infection was fourfold higher among participants with formal employment (AOR 3.8, 95% CI, 1.53-9.40). Use of water from wells/rivers (AOR 2.2, 95% CI, 1.07-4.45), drinking untreated water (AOR 2.6, 95% CI, 1.21-5.48) and often eating at a restaurant (AOR 3.4, 95% CI, 1.28-8.93) had increased odds of Salmonella infection. Likewise, having abdominal pain (AOR 8.5, 95% CI, 1.81-39.78) and diarrhea (AOR 2.3, 95% CI, 1.12-4.68) were independent symptoms that predict Salmonella infection. Conclusion There is a high prevalence of Salmonella infection among people who report gastrointestinal complaints and it is clinically predicated by diarhoea and abdominal pain. Employed participants and those eating at restaurant and drinking unsafe water had higher risk of infection. Salmonella spp causing gastroenteritis has developed resistance to commonly used antibiotics.

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Antibiotics Susceptibility Pattern of Bacteria Isolated from Ready-To-Eat Vegetables Sold in Mohammadu Gumi Market, Kaduna, Nigeria

Nigerian Journal of Pharmaceutical Research ◽

10.4314/njpr.v16i2.15s ◽

2021 ◽

Vol 16 (2) ◽

pp. 131-137

Author(s):

A.F. Obajuluwa ◽

A. Kefas ◽

S.K. Parom

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Susceptibility ◽

Diffusion Method ◽

Resistance Pattern ◽

Resistant Bacteria ◽

Antibiotics Resistance ◽

Salmonella Spp ◽

Methicillin Resistant ◽

Agar Disc

Background: Freshly consumed vegetables are considered to have more nutritional value than cooked ones. However, they are a potential source of foodborne illnesses due to possible microbial contamination, this poses safety threat.Objectives: This study was carried out to determine the bacteriological quality of ready-to-eat vegetables sold in Mohammadu Gumi market, Kaduna and to determine the antibiotics resistance pattern of the bacteria isolates.Methods: A total of 40 samples of cabbage were collected. Gram staining, growth on selective media and biochemical tests were carried out to identify the isolates. Antimicrobial susceptibility testing was done using the Kirby-Bauer agar disc diffusion method. Methicillin resistant Staphylococcus aureus isolates were detected with the use of cefoxitin disc agar diffusion test.Results: A total of 46 bacteria isolates were obtained with a total colony count range from 4 – 9 x 106CFU/ml. The following bacteria were isolated: aureus (41.3%), Escherichia coli (28.3%), Salmonella spp (19.6%), Pseudomonas aeruginosa (8.7%) and Serratia spp (2.2%). The result of antimicrobial susceptibility test showed thatthe isolates were highly susceptible to ofloxacin: Staphylococcus aureus, Pseudomonas aeruginosa, Serratia spp (100%), E. coli (92.3%) and Salmonella spp (87.5%). The isolates were all resistant to penicillin and ampicillin while 89.5% of the Staphylococcus aureus isolates were phenotypically methicillin resistant Staphylococcus aureus.Conclusion: This study showed that the vegetables (cabbage) were highly contaminated with antibiotic resistant bacteria, this can be a source of infection to the consumers and a potential means of transmitting multidrug resistant bacteria strains in the community.

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Molecular Detection and Antimicrobial Susceptibility of Salmonella Species in Chickens

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2021.1009.064 ◽

2021 ◽

Vol 10 (9) ◽

pp. 560-566

Author(s):

Gloria Sugun Ehizibolo David ◽

Ebenezer Bukola Ogundejii Kaikabo Ahmed ◽

Agada O. Godwin Benshak Audu ◽

Sugun Manasa

Keyword(s):

Antimicrobial Susceptibility ◽

Biochemical Analysis ◽

Molecular Detection ◽

Biochemical Tests ◽

Salmonella Spp ◽

Selective Media ◽

Antimicrobial Sensitivity ◽

Cultural Properties ◽

Plateau State ◽

Antimicrobial Sensitivity Test

The study was intended for molecular detection of Salmonella Spp isolated from chicken. A total of 160 samples comprising of 40 liver, 40 spleen, 40 lungs and 40 intestines were collected directly into buffered peptone in universal bottles at the poultry slaughter houses of the four districts in Jos South Local Government area of Plateau State. The samples were enriched in 10 ml of Rappaport-Vassiliades broth and cultured onto Xylems Lysine Deoxycholate (XLD) agar for the isolation of bacteria. The isolated bacteria were identified by studying staining characteristics, cultural properties on different selective media, biochemical tests, catalase and coagulase test, and finally by PCR. Out of 160 samples, 65 (41%) samples were found positive for Salmonella Spp on XLD and 24 (37%) positive by biochemical analysis. Two(2) Salmonella isolates were amplified by 942 bp gene based PCR. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the Salmonella isolates were resistant to amoxycillin (100%) and erythromycin (100%), gentamicin (100%), Clindamycin (100%) streptomycin (100%), tetracycline (100%), sulphamethoxazole/trimethoprim (100%) but sensitive to Ceftiofur (100%).

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Isolation, Identification, and Antimicrobial Susceptibility Test of Bacteria from Vulva Swab of African Pygmy Hedgehog (Atelerix albiventris) and Sunda Porcupine (Hystrix javanica)

BIO Web of Conferences ◽

10.1051/bioconf/20213306009 ◽

2021 ◽

Vol 33 ◽

pp. 06009

Author(s):

Anindya Dwi Ash-Santri ◽

Vinsa Cantya Prakasita ◽

Yosua Kristian Adi ◽

Teguh Budipitojo ◽

Agnesia Endang Tri Hastuti Wahyuni

Keyword(s):

Escherichia Coli ◽

Antimicrobial Susceptibility ◽

Proteus Mirabilis ◽

Susceptibility Test ◽

Penicillin G ◽

Diffusion Method ◽

Antimicrobial Susceptibility Test ◽

Biochemical Tests ◽

Disk Diffusion Method ◽

Selective Media

Atelerix albiventris and Hystrix javanica are currently traded as pets or consumed in Indonesia, but there has been no research about bacteria from the vulva swab before. This research aims to isolate and identify bacteria from the vulva swabs of Atelerix albiventris and Hystrix javanica, and identify their antibiotic susceptibility. Samples were isolated by blood agar plates and selective media and identified by biochemical tests. Kirby Bauer’s disk diffusion method was used for the antimicrobial susceptibility test. The result showed that from Atelerix albiventris was isolated and identified Escherichia coli and Proteus mirabilis, while from Hystrix javanica was isolated and identified Escherichia coli. The identified Escherichia coli was sensitive to Amikacin, Amoxycillin, Ampicillin, Enrofloxacin, Fosfomycin, Chloramphenicol, Tetracycline, Trimethoprim, and Kanamycin; intermediate to Streptomycin; and resistant to Erythromycin and Penicillin G. The identified Proteus mirabilis was sensitive to Amikacin, Amoxycillin, Kanamycin, Enrofloxacin, and Fosfomycin; and resistant to Erythromycin, Penicillin G, Streptomycin, Ampicillin, Chloramphenicol, Tetracycline, and Trimethoprim. This research concludes that Escherichia coli and Proteus mirabilis were isolated from vulva swab of Atelerix albiventris and Hystrix javanica, Escherichia coli was sensitive to eight antibiotics, while Proteus mirabilis was sensitive to five antibiotics.

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Factors Associated with Salmonella Infection in Patients with Gastrointestinal Complaints Seeking Health Care at Regional Hospital in Southern Highland of Tanzania

10.21203/rs.2.12908/v2 ◽

2019 ◽

Author(s):

Fadhili Ashery Ngogo ◽

Agricola Joachim ◽

Ahmed M Abade ◽

Susan F Rumisha ◽

Mucho M Mizinduko ◽

...

Keyword(s):

Abdominal Pain ◽

Antimicrobial Susceptibility ◽

Public Health Problem ◽

Regional Hospital ◽

Diffusion Method ◽

Salmonella Infection ◽

Salmonella Spp ◽

Factors Associated ◽

Gastrointestinal Complaints ◽

Southern Highland

Abstract Background Salmonellosis remains an important public health problem globally. The disease is among the leading causes of morbidity and mortality in developing countries that experience poor hygiene and lack of access to clean and safe water. There was an increase in reported cases of Salmonellosis in the Njombe region Southern Highland of Tanzania between 2015 and 2016, based on clinical diagnosis. Nevertheless, little is known about the factors contributing to the transmission of this disease in the region. This study was conducted to determine the prevalence, antimicrobial susceptibility, and factors associated with Salmonella infection among patients who report gastrointestinal complaints. Methods A cross-sectional study was conducted from December 2017 to February 2018 among patients with gastrointestinal complaints at Kibena Regional Hospital. Stool samples were submitted for culture on Xylose Lysine Deoxycholate Agar, intended for isolation of Salmonella spp. Identification was based on conventional biochemical tests and serotyping to differentiate typhoid and non-typhoid Salmonella. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disc diffusion method. Multivariable logistic regression analysis was performed to examine the factors independently associated with Salmonella infection after adjustment for other explanatory variables. Results The prevalence of Salmonella infection among participants with gastrointestinal complaints was 16.5% (95% CI: 12.7-21.1). All isolates were sensitive to ceftriaxone and ciprofloxacin, whereas 27.8% and 100% were resistant to co-trimoxazole and ampicillin respectively. The odd of Salmonella infection was fourfold higher among participants with formal employment (AOR 3.8, 95% CI, 1.53-9.40). Use of water from wells/rivers (AOR 2.2, 95% CI, 1.07-4.45), drinking untreated water (AOR 2.6, 95% CI, 1.21-5.48) and often eating at a restaurant (AOR 3.4, 95% CI, 1.28-8.93) had increased odds of Salmonella infection. Likewise, having abdominal pain (AOR 8.5, 95% CI, 1.81-39.78) and diarrhea (AOR 2.3, 95% CI, 1.12-4.68) were independent symptoms that predict Salmonella infection. Conclusion There is a high prevalence of Salmonella infection among people who report gastrointestinal complaints and it is clinically predicated by diarhoea and abdominal pain. Employed participants and those eating at restaurant and drinking unsafe water had higher risk of infection. Salmonella spp causing gastroenteritis has developed resistance to commonly used antibiotics.

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Factors Associated with Salmonella Infection in Patients with Gastrointestinal Complaints Seeking Health Care at Regional Hospital in Southern Highland of Tanzania

10.21203/rs.2.12908/v3 ◽

2020 ◽

Author(s):

Fadhili Ashery Ngogo ◽

Agricola Joachim ◽

Ahmed M Abade ◽

Susan F Rumisha ◽

Mucho M Mizinduko ◽

...

Keyword(s):

Abdominal Pain ◽

Antimicrobial Susceptibility ◽

Public Health Problem ◽

Regional Hospital ◽

Diffusion Method ◽

Salmonella Infection ◽

Salmonella Spp ◽

Factors Associated ◽

Gastrointestinal Complaints ◽

Southern Highland

Abstract Background Salmonellosis remains an important public health problem globally. The disease is among the leading causes of morbidity and mortality in developing countries that experience poor hygiene and lack of access to clean and safe water. There was an increase in reported cases of Salmonellosis in Njombe region, Southern Highland of Tanzania between 2015 and 2016, based on clinical diagnosis. Nevertheless, little is known about the factors contributing to the transmission of this disease in the region. This study was conducted to determine the prevalence, antimicrobial susceptibility, and factors associated with Salmonella infection among patients who report gastrointestinal complaints. Methods A cross-sectional study was conducted from December 2017 to February 2018 among patients with gastrointestinal complaints at Kibena Regional Hospital. Stool samples were submitted for isolation of Salmonella spp. Identification was based on conventional biochemical tests and serotyping to differentiate typhoid and non-typhoid Salmonella (NTS). Antimicrobial susceptibility was performed using the Kirby-Bauer disc diffusion method. Multivariable logistic regression analysis was performed to examine the factors independently associated with Salmonella infection. Results The prevalence of Salmonella infection among participants with gastrointestinal complaints was 16.5% (95% CI: 12.7-21.1) of them, 83.7%, 95% CI: 70.9-91.5 were NTS while 16.3%, 95% CI: 8.5-29.0 were Typhoid Salmonella species. All isolates were sensitive to ceftriaxone and ciprofloxacin, whereas 27.8% and 100% were resistant to co-trimoxazole and ampicillin respectively. The odd of Salmonella infection was fourfold higher among participants with formal employment (AOR 3.8, 95% CI, 1.53-9.40). Use of water from wells/rivers (AOR 2.2, 95% CI, 1.07-4.45), drinking untreated water (AOR 2.6, 95% CI, 1.21-5.48) and often eating at a restaurant (AOR 3.4, 95% CI, 1.28-8.93) had increased odds of Salmonella infection. Likewise, having abdominal pain (AOR 8.5, 95% CI, 1.81-39.78) and diarrhea (AOR 2.3, 95% CI, 1.12-4.68) were independent symptoms that predict Salmonella infection. Conclusion There is a high prevalence of Salmonella infection among people who report gastrointestinal complaints and it is clinically predicated by diarhoea and abdominal pain. Employed participants and those eating at restaurant and drinking unsafe water had higher risk of infection. Salmonella spp causing gastroenteritis has developed resistance to commonly used antibiotics.

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