scholarly journals Insect Crude Thoraxial Antigen-G Extracted from Apis mellifera to Enhance Serum Immunoglobulin of Goats: An Entomology Contribution in Animal Science

2019 ◽  
Vol 20 (2) ◽  
pp. 133
Author(s):  
Wisje Lusia Toar ◽  
Laurentius Rumokoy ◽  
Ivonne Maria Untu ◽  
Geertruida Assa
Keyword(s):  
Apis Mellifera ◽  
Serum Antibody ◽  
Treated Group ◽  
Serum Immunoglobulin ◽  
Control Group ◽  
Immunoglobulin Level ◽  
Animal Science ◽  
Serum Immunoglobulin Level ◽  
Pathogenic Microbes

This research was conducted to evaluate the influence of insect crude thoraxial antigen-G (CTA) extracted from Apis mellifera L. (Hymenoptera: Apidae) to enhance goat’s serum immunoglobulin level. The first part of this study was the determination of insect CTA proportion level. The insects were collected from four different places: Tomohon, Minahasa, North-Minahasa and Manado areas. The second part of the study was the application of A. mellifera CTA substance on serum immunoglobulin level classification. In this part, twelve young goats handled with traditional maintenance.  The animals experiment were divided in two groups: control group and the other treated with 100 µg CTA extract. The proportion of serum immunoglobulins level of goats was detected at 14th days after immunization with insects CTA extract, and compared with the animals immunoglobulin levels at the starting day of treatment.  The data of CTA extract proportion level of the insects collected were subjected to statistically analysis using the general linear model (GLM) procedure of SPSS 22. Concerning the classification level of the animal treated with CTA was statistically analyzed according to Mann-Whitney test. The results showed that the proportion level of thoraxial antigens-G of A. mellifera from all areas observed were not significant different (P>0.05). This crude thoraxial antigens-G of this insect were able to increase serum antibody level of the experiment animal after 14 days of immunization. The immunoglobulin level qualification of animals in treated group were significant higher (P<0.05) than in control group. We concluded that the CTA extract of the Apis mellifera could be empowered to improve the young goat immunity against the pathogenic microbes in their environment. 

scholarly journals Antigen-G of Larva Serum of Musca domestica as an Immunoglobulin Production Promoter in Goats under an Organic Maintenance

2020 ◽  
Vol 22 (1) ◽  
pp. 31-36
Author(s):  
Laurentius Rumokoy ◽  
Hengky Kiroh ◽  
Ivonne Maria Untu ◽  
Geertruida Assa ◽  
Santie Turangan ◽  
...  
Keyword(s):  
Serum Antibody ◽  
Treated Group ◽  
Serum Immunoglobulin ◽  
Control Group ◽  
Blood Collection ◽  
Immunoglobulin Production ◽  
Insect Rearing ◽  
Quality Classification ◽  
Serum Protein Level ◽  
Organically Managed

This experiment aimed to reveal the effect of serum G-antigen of M. domesticainsect larvae (LAS) as promoter antigen on serum immunoglobulin production in organically managed goat livestock. This study used 12 local goats which were divided into two groups: as a control group and treated group receiving treatment. Insect rearing was conducted to obtain the larvae, the antigen-G was then extracted from the larvae to be used as promoter antigen to enhance the serum antibody production which was subcutaneously immunized in experimental goats and incubated for a period of 14 days. Blood collection of 2.5 ml was taken through the jugular vein and then carried out a quantification of serum protein level indicating the antibody proportion of goats. The data of the LSA extract proportion level were statistically analyzed with t-test, and the quality classification level of serum immunoglobulin of animal groups were statistically analysed. The results showed that the serum of animal treated with LSA of M. domesticaresulted in a higher level of immunoglobulin (P <0.01) compared to the control. We conclude that the antigen-g substance (LSA) could support the efforts to improve the production of organic goat livestock by increasing the total level of antibodies circulating in the blood.

Effect of Apitherapy in Piglets with Preweaning Diarrhea

2003 ◽  
Vol 31 (02) ◽  
pp. 321-326 ◽  
Author(s):  
Seok Hwa Choi ◽  
Seong Koo Cho ◽  
Seong Soo Kang ◽  
Chun Sik Bae ◽  
Young Hoon Bai ◽  
...  
Keyword(s):  
Body Weight ◽  
Apis Mellifera ◽  
Standard Dose ◽  
Treated Group ◽  
Bee Venom ◽  
Control Group ◽  
Main Concern ◽  
Antibacterial Drug ◽  
Control Groups ◽  
Bacterial Diarrhea

This study was designed to examine the therapeutic effect of honeybee (Apis mellifera L.) venom in piglets with bacterial diarrhea. Comparison between bee venom- and drug-treated groups was our main concern in the present study. Preweaning piglets were assigned to treated and non-treated control groups. In the treated group, 47 piglets were acupunctured with the worker honeybee once a day for three consecutive days. Two acupoints, GV-1 (Jiao-chao) and ST-25 (Hai-men), were selected for apitherapy. In the control group, 44 piglets were intramuscularly injected with a standard dose of a known antibacterial drug, colistin sulfate (300,000 IU/kg of body weight), and an antidiarrheal drug (berberine, 2 ml/kg) once a day for three consecutive days. At post-treatment, 90.9% of the control piglets and 93.6% of piglets in the treated group recovered from bacterial diarrhea. Bee acupuncture therapy did not show any side effects such as allergy, intoxication, hemorrhage or infection. It is concluded that bee venom therapy was effective in controlling bacterial diarrhea in preweaning piglets.

scholarly journals Amelioratory Effect of Nanoconjugated Vancomycin on Spleen during VRSA-Induced Oxidative Stress

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Subhankari Prasad Chakraborty ◽  
Santanu KarMahapatra ◽  
Sumanta Kumar Sahu ◽  
Panchanan Pramanik ◽  
Somenath Roy
Keyword(s):  
Oxidative Stress ◽  
Reduced Glutathione ◽  
Treated Group ◽  
Control Group ◽  
Myeloperoxidase Activity ◽  
Viable Bacteria ◽  
Antioxidant Effects ◽  
Potential Use

Objective. The aim of the present study was to evaluate the possible antioxidant effects of nanoconjugated vancomycin against VRSA infection on select makers of oxidative damage and antioxidant status in spleen. Methods. A coagulase-positive VRSA strain was used for this study. VRSA infection was developed in Swiss mice by intraperitoneal injection of 5 × 106 CFU/mL bacterial solutions. VRSA-infected mice were treated with nanoconjugated vancomycin at its effective dose for 10 days. After decapitation, blood was used for determination of viable bacteria count and spleen was excised from control and experimental groups, homogenized and used for different biochemical estimations. Results. Nitrate level, myeloperoxidase activity, lipid peroxidation, protein oxidation, oxidized glutathione, and DNA fragmentation level were increased significantly (P<0.05) in spleen of VRSA-infected group as compared to control group, and reduced glutathione level, activity of SOD, CAT, GPx, GR, and GST were decreased significantly (P<0.05); which were increased or decreased significantly (P<0.05) near to normal in nanoconjugated vancomycin-treated group. Conclusion. These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VRSA-infection-induced oxidative stress and DNA damage in spleen.

scholarly journals Effects ofSalvadora persicaExtract on the Hematological and Biochemical Alterations against Immobilization-Induced Rats

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Kholoud S. Ramadan ◽  
Salha A. Alshamrani
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Hdl Cholesterol ◽  
Treated Group ◽  
Control Group ◽  
Serum Samples ◽  
Salvadora Persica ◽  
Biochemical Alterations ◽  
Hematological And Biochemical Parameters

A total of 24 rats were divided into 4 groups: control, stress, extract alone, and stress + extract (n=6each), for total 21 days of treatment. The immobilization stress was induced in rats by putting them in 20 cm × 7 cm plastic tubes for 2 h/day for 21 days. Rats were postorally treated withSalvadora persicaat a dose of 900 mg/kg body weight via intragastric intubations. At the end of the test period, hematological and biochemical parameters were determined in blood and serum samples with determination of vital organs weights. The vital organ weights were not significantly affected in stressed rats as compared to control rats. Compared to the control group, the stress treated group showed significances in several hematological parameters, including decreases in WBC, RBC, and PLT counts. Furthermore, in comparison to the control group, the stress group showed significantly increased blood glucose, serum total cholesterol, LDL-cholesterol, and triacylglycerols levels and decreased HDL-cholesterol level. The hematological and biochemical parameters in the stress + extract treated group were approximately similar to control group. The SP extract restored the changes observed following stress treatment.

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