scholarly journals Saliva and Serum Ghrelin and Obestatin in Iron Deficiency Anemia Patients

2018 ◽  
Author(s):  
Kader Ugur ◽  
Suleyman Aydin ◽  
Emir Donder ◽  
İbrahim Sahin ◽  
Meltem Yardim ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Binding Capacity ◽  
Total Iron ◽  
Deficiency Anemia ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Iron Treatment ◽  
Iron Binding Capacity ◽  
Serum Ghrelin

Ghrelin and obestatin, two antagonist peptide hormones, are purportedly involved in stimulating appetite and controlling energy balance in humans. Serum ghrelin level is also associated with iron deficiency anemia (IDA), but no study has yet been made of the obestatin level in patients with IDA, even though both hormones are a single gene product. Therefore, the purpose of this investigation is to see whether there is a link between IDA and these two hormones among other hematological parameters in patients with IDA. To measure ghrelin and obestatin, human saliva and serum were collected from 30 women with IDA, aged 31.7 ± 10.7 years, and 30 control women, aged 30.2 ± 8.0 years, with repeated collection of samples over a period of 1 week and 1 month. Saliva and serum ghrelin levels were measured by ELISA. Serum hemoglobin, ferritin, hematocrit and total iron-binding capacity (TIBC) values were determined with an Olympus AU2700.  Saliva and serum ghrelin and obestatin levels were significantly lower in the IDA group compared with controls; these levels increased slightly above baseline with iron treatment, but remained below the control values. Furthermore, and as expected, serum hemoglobin, ferritin, and hematocrit levels were significantly increased with iron treatment, while total iron-binding capacity decreased compared to baseline concentrations. The findings suggest that IDA might be linked to imbalance of circulating (serum) and non-circulating (saliva) ghrelin and obestatin levels. Decreased ghrelin and obestatin might destroy iron homeostasis through its effect on intestinal absorption. Measuring these hormone levels might be useful for monitoring the response to iron treatment. Also, serum and saliva levels for both hormones were well correlated. Thus, using saliva in place of serum for monitoring the two hormones should minimize inconvenience and patient discomfort.

Immunological Measurement of Transferrin Compared with Chemical Measurement of Total Iron-Binding Capacity

1975 ◽  
Vol 21 (8) ◽  
pp. 1063-1066 ◽  
Author(s):  
Swei H Tsung ◽  
Waldemar A Rosenthal ◽  
Karen A Milewski
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Binding Capacity ◽  
Total Iron ◽  
Deficiency Anemia ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Chronic Disorders ◽  
Birth Control Pills ◽  
Iron Binding Capacity

Abstract Because of uncertainty as to the molecular weight of transferrin, a previous comparison [Von der Heul et al., Clin. Chim. Acta 38, 347 (1972)] between transferrin content of serum and total iron-binding capacity cannot be definitive. We found a conversion factor for expressing transferrin as iron-binding capacity by measuring the maximum amount of iron bound by 1 mg of transferrin. We compared the resulting calculated value with values obtained by three other methods for measuring total iron-binding capacity. We agree with the previous observation that the latter, as measured radioisotopically, give higher results than would be judged from the transferrin content but the same as those for two chemical methods. The diffusion rate of transferrin in agar was the same irrespective of the degree of iron saturation. Serum transferrin concentrations were low in patients with anemia resulting from malignancy, chronic disorders, and cirrhosis of the liver, and high or normal in patients with iron deficiency anemia and in pregnant women or women who were taking birth-control pills. Measurement of transferrin concentration can be used to distinguish iron deficiency anemia from anemia resulting from chronic disorders, but offers no advantages over existing methods for estimating total ironbinding capacity.

Serum iron and total iron-binding capacity compared with serum ferritin in assessment of iron deficiency.

1981 ◽  
Vol 27 (2) ◽  
pp. 276-279 ◽  
Author(s):  
F Peter ◽  
S Wang
Keyword(s):  
Iron Deficiency ◽  
Serum Ferritin ◽  
Serum Iron ◽  
Binding Capacity ◽  
Transferrin Saturation ◽  
Total Iron ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Iron Deficient ◽  
Iron Binding Capacity

Abstract Ferritin values for 250 selected sera were compared with values for iron, total iron-binding capacity (TIBC), and transferrin saturation, to assess the potential of the ferritin assay for the detection of latent iron deficiency. The specimens were grouped (50 in each group) according to their values for iron and TIBC. In Group 1 (low iron, high TIBC) the saturation and ferritin values both indicated iron deficiency in all but one. In the 100 specimens of Groups 2 (normal iron, high TIBC) and 4 (normal iron, high normal TIBC), the saturation values revealed 16 iron-deficient cases, the ferritin test 55. For Groups 3 (low iron, normal TIBC) and 5 (low iron, low TIBC), the ferritin test revealed fewer cases of iron deficiency than did the saturation values (37 cases vs 51 cases, in the 100 specimens). Evidently the ferritin test detects iron deficiency in many cases for whom the serum iron and TIBC tests are not positively indicative. The correlation of serum ferritin with iron, TIBC, and transferrin saturation in the five groups was good only in the case of specimens for which the TIBC was normal; if it was abnormal the correlation was very poor.

Saliva and serum ghrelin and obestatin in iron deficiency anemia patients

2018 ◽  
Vol 42 (5) ◽  
pp. 183-188 ◽  
Author(s):  
Kader Ugur ◽  
Suleyman Aydin ◽  
Emir Donder ◽  
İbrahim Sahin ◽  
Meltem Yardim ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Binding Capacity ◽  
Single Gene ◽  
Hematological Parameters ◽  
Human Saliva ◽  
Deficiency Anemia ◽  
Enzyme Linked Immunosorbent Assay ◽  
Iron Treatment ◽  
Serum Ghrelin

AbstractBackgroundSerum ghrelin level is also associated with iron deficiency anemia (IDA), but no study has yet been published on the obestatin level in patients with IDA, even though both hormones are a single gene product. Therefore, the purpose of this investigation was to determine whether there is a link between IDA and these two hormones among other hematological parameters in patients with IDA.MethodsTo measure ghrelin and obestatin, human saliva and serum were collected from 30 women with IDA and 30 control women with repeated collection of samples over a period of 1 week and 1 month. Saliva and serum ghrelin levels were measured by enzyme-linked immunosorbent assay.ResultsSaliva and serum ghrelin and obestatin levels were significantly lower in the IDA group compared with controls; these levels increased slightly above baseline with iron treatment, but remained below the control values. Serum hemoglobin (Hb), ferritin and hematocrit (Hct) levels significantly increased with iron treatment, while total iron-binding capacity (TIBC) decreased compared to baseline concentrations.ConclusionsThe findings suggest that IDA might be linked to imbalance of circulating (serum) and non-circulating (saliva) ghrelin and obestatin levels. Using saliva in place of serum for monitoring the two hormones should minimize inconvenience and patient discomfort.

scholarly journals Hemodialysis Duration with Levels of Hemoglobin, Hematocrit, Serum Iron and Total Iron Binding Capacity on Chronic Kidney Disease

2021 ◽  
Vol 19 (2) ◽  
pp. 197-205
Author(s):  
Supriati Wila Djami ◽  
Marni Tangkelangi
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Laboratory Tests ◽  
Serum Iron ◽  
Binding Capacity ◽  
Total Iron ◽  
Deficiency Anemia ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Iron Binding Capacity

The correlation of the length of time undergoing hemodialysis with the levels of hemoglobin, hematocrit, serum iron and total iron binding capacity in patients with chronic kidney disease at RSUD Prof. Dr. W. Z. Johannes Kupang. People with chronic kidney disease who are performing Hemodialysis suffer from anemia. Blood loss during the hemodialysis process is affected by frequent blood sampling for laboratory tests. To diagnose the occurrence of iron deficiency, anemia laboratory tests such as complete blood, serum iron (SI), TIBC, transferrin saturation, and serum ferritin can be conducted. The objective of this study is to determine the correlation between the length of undergoing Hemodialysis with the levels of Hemoglobin, Hematocrit, Serum Iron, and Total Iron Binding Capacity (TIBC) in patients with chronic kidney disease in RSUD Prof. Dr.W. Z Johannes Kupang. This study employed an anon-reactive research design or unobtrusive research based on secondary data derived from medical record records at RSUD Prof. Dr.W. Johannes in August - September 2019 on 92 hemodialysis patients who met the inclusion criteria. The collected data were calculated with the SPSS application, while the correlation analysis between variables was performed by administering a linear regression analysis test. Ninety-two (92) respondents were obtained by employing the purposive sampling technique. The majority of patients with chronic kidney disease undergoing Hemodialysis were men (57.6%) with ages 39 - 59 years (63.0%), and the majority of patients undergoing Hemodialysis in patients with chronic kidney failure (CKD) were <12 months (41.3%). It was discovered that there was no relationship between the length of Hemodialysis with hemoglobin, hematocrit, serum iron, and total iron-binding capacity levels in patients with chronic kidney disease (CKD).

scholarly journals Peran Reticulocyte Hemoglobin Content (Ret-He) dalam Mendeteksi Defisiensi Besi pada Anak

Sari Pediatri ◽  
2019 ◽  
Vol 20 (5) ◽  
pp. 316
Author(s):  
Ika Maya Sandy ◽  
Murti Andriastuti
Keyword(s):  
Iron Deficiency ◽  
Binding Capacity ◽  
Total Iron ◽  
Google Scholar ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Hemoglobin Content ◽  
Level Of Evidence ◽  
Iron Binding Capacity ◽  
Reticulocyte Hemoglobin Content

Latar belakang. Defiseinsi besi (DB) didefinisikan sebagai penurunan total kandungan besi dalam tubuh yang ditandai dengan turunnya kadar feritin atau saturasi transferin. Gangguan perkembangan neurokognitif yang timbul akibat DB bersifat permanen. Identifikasi adanya DB penting untuk memulai terapi besi untuk mencegah komplikasi jangka panjang.Tujuan. Mengetahui peran Ret-He dalam mendeteksi DB pada anak dibandingkan dengan pemeriksaan biomarker penanda status besi lain (feritin, saturasi transferin, kadar besi serum, dan total iron binding capacity (TIBC)).Metode. Penelusuran pustaka database elektronik, yaitu Pubmed, Cochrane, ProQuest dan Google scholar dengan kata kunci “iron deficiency”, “AND” “reticulocyte hemoglobin  content”, “AND” “children”.Hasil. Penelusuran literatur diperoleh 4 artikel yang terpilih kemudian dilakukan telaah kritis. Studi oleh Mateos dkk, dengan level of evidence 1c, diperoleh nilai cut-off: ≤ 25 pg, memiliki sensitivitas tertinggi dibanding studi lainnya, yaitu 94% dan spesifisitas 80%, NDP 54%, NDN 97%. Studi lain menentukan nilai cut-off yang berbeda-beda sehingga mempengaruhi nilai sensitivitas, spesifisitas, nilai duga positif dan negatif.Kesimpulan. Berdasarkan penelitian ilmiah yang telah dipaparkan dapat disimpulkan bahwa Ret-He dapat digunakan sebagai parameter untuk mendeteksi DB pada anak.

scholarly journals CLINICAL PROFILES

Blood ◽  
1968 ◽  
Vol 32 (4) ◽  
pp. 690-695 ◽  
Author(s):  
RALPH O. WALLERSTEIN
Keyword(s):  
Iron Deficiency ◽  
Intravenous Injection ◽  
Binding Capacity ◽  
Total Iron ◽  
Deficiency Anemia ◽  
Iron Dextran ◽  
Proper Place ◽  
Iron Treatment ◽  
Clinical Profiles ◽  
Iron Binding Capacity

Abstract Experience by many observers in the last few years has shown that intravenously administered iron dextran is a useful and safe agent in the management of iron-deficiency anemia. The conscientious physician, who has diagnosed iron deficiency by the absence of hemosiderin in marrow or by the characteristic serum values for iron and total iron-binding capacity, may well prefer to inject the calculated correct amount of iron rather than depend on the capriciousness of the patient’s gastrointestinal tract or pill-taking habits. In clinical situations in which the doctor-patient contacts are severely limited in number, intravenous injection may be the only reliable way to administer the proper amount of iron. Treatment by intravenous injection, only after employing firm diagnostic criteria, may restore iron to its proper place in the physician’s armamentarium and elevate it from buckshot to bullet.

The Assessment of Iron Deficiency in Pregnancy

1969 ◽  
Vol 14 (6) ◽  
pp. 209-214 ◽  
Author(s):  
J. Bonnar ◽  
A. Goldberg
Keyword(s):  
Iron Deficiency ◽  
Haemoglobin Level ◽  
Early Pregnancy ◽  
Binding Capacity ◽  
Total Iron ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Not Given ◽  
Iron Binding Capacity ◽  
In Pregnancy

Serial observations were made on two groups of women from early pregnancy to term to assess the effect of pregnancy on the iron status. The first group had no evidence of iron deficiency in early pregnancy and were not given supplementary iron. The second group of patients were a random selection and each patient was given a regular supply of iron tablets. The cooperation of the patient in taking the iron was verified by stool tests. The measurements of the serum iron, total iron binding capacity (transferrin), and erythrocyte porphyrins in addition to the other haematological investigations indicated that in the patients not given iron and those who did not take the supplementary iron the haemoglobin level gradually decreased, and iron deficiency was present. In the patients regularly taking supplementary iron the haemoglobin level was over 12 g. per 100 ml. at term, and the biochemical findings showed no evidence of iron deficiency. The total iron-binding capacity gradually increased during pregnancy with and without iron deficiency. Red cell morphology and the mean haemoglobin concentration were not sensitive indices of iron deficiency. The results of the study suggest that ‘physiological’ anaemia does not exist in pregnancy and reduced haemoglobin values in late pregnancy are most likely the result of an inadequate iron intake.

SERUM IRON AND IRON-BINDING CAPACITY OF THE SERUM IN CHILDREN WITH SEVERE MEDITERRANEAN (COOLEY'S) ANEMIA

PEDIATRICS ◽  
1950 ◽  
Vol 5 (5) ◽  
pp. 799-807
Author(s):  
CARL H. SMITH ◽  
THOMAS R. C. SISSON ◽  
WILLIAM H. FLOYD ◽  
SHIRLEY SIEGAL
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Binding Protein ◽  
High Serum ◽  
Total Iron ◽  
Deficiency Anemia ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Normal Children ◽  
Iron Binding Capacity

The blood of children with severe Mediterranean anemia was found to have a high serum iron and an absence of a latent or unbound iron capacity of the serum. Whereas serum iron levels comprise approximately one third of the total iron-binding capacity in normal children and adults, the two values are equal in the severe form of Mediterranean anemia. The iron-binding capacity of the serum in this condition is therefore fully saturated and quantitatively lower than in normal children. The absence of a measurable latent iron-binding capacity does not depend entirely on the liberation of iron from exogenously administered blood. Comparable changes were also noted in two nonanemic adult carriers possessing the trait of the disease, in two children with the mild form not requiring transfusions, and in one severely anemic child with Mediterranean anemia who had never been transfused. Similar changes occurred in three patients with spherocytic anemia and in five children with sickle cell anemia, one of whom had never been transfused. In every instance therefore of the advanced form of Mediterranean anemia and in some of the nonanemic and mildly anemic forms of this disease, a fully saturated metal-binding protein is present in total amounts usually less than those found in normal children. In contrast, children with iron deficiency anemia manifest a reduction in percentage saturation of iron-binding protein, an increased level of unbound latent iron-binding capacity and a markedly elevated total iron-binding capacity. The failure to demonstrate a latent iron-binding capacity of the serum in asymptomatic persons adds another criterion to those already established for the identification of carriers of the trait of Mediterranean anemia who constitute the source of its transmission. The presence of bilirubin in increased concentrations in the serum of patients with severe Mediterranean anemia does not interfere with its capacity to combine with iron. The capacity for binding iron was restored when Fraction IV-7 was added to samples of icteric sera of patients in whom no capacity had previously been demonstrated. The high serum iron and absence of latent iron-binding capacity observed in children with severe Mediterranean anemia receiving multiple transfusions correspond to the changes regarded as diagnostic of transfusion hemosiderosis and hemochromatosis. While these changes remain unaltered in Mediterranean anemia, their reversibility has been demonstrated in two children with hemophilia who received weekly transfusions of small amounts of blood. Following cessation of this form of therapy a decrease of serum iron and a gradual restoration of iron-binding capacity were noted.

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