scholarly journals Insights into Nitric Oxide-Mediated Water Balance, Antioxidant Defence and Mineral Homeostasis in Rice (Oryza sativa L.) under Chilling Stress

2020 ◽  
Author(s):  
Abdullah Al Mamun Sohag ◽  
Md Tahjib-Ul-Arif ◽  
Sonya Afrin ◽  
Md Kawsar Khan ◽  
Md. Abdul Hannan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Oryza Sativa ◽  
Molecular Mechanisms ◽  
Oryza Sativa L ◽  
Chilling Stress ◽  
Soluble Sugar ◽  
Chilling Injury ◽  
Chilling Tolerance ◽  
No Donor ◽  
Mineral Homeostasis

Being a chilling-sensitive staple crop, rice (Oryza sativa L.) is vulnerable to climate change. The competence of rice to withstand chilling stress should, therefore, be enhanced through technological tools. The present study employed chemical intervention like application of sodium nitroprusside (SNP) as nitric oxide (NO) donor and elucidated the underlying molecular mechanisms of NO-mediated chilling tolerance in rice. At germination stage, germination indicators were interrupted by chilling stress (5.0 ± 1.0°C for 8 h day‒1), while pretreatment with 100 μM SNP markedly improved the indicators. At seedling stage (14-day-old), chilling stress caused stunted growth with visible toxicity along with alteration of biochemical markers, for example, increase in oxidative stress markers (superoxide, hydrogen peroxide, and malondialdehyde) and osmolytes (total soluble sugar; proline and soluble protein content, SPC), and decrease in chlorophyll (Chl), relative water content (RWC), and antioxidants. However, NO application attenuated toxicity symptoms with improving growth performance which might be attributed to enhanced activities of antioxidants, mineral contents, Chl, RWC and SPC. Furthermore, principal component analysis indicated that water imbalance and increased oxidative damage were the main contributors to chilling injury, whereas NO-mediated mineral homeostasis and antioxidant defense were the critical determinants for chilling tolerance in rice. Collectively, our findings revealed that NO protects against chilling stress through valorizing cellular defense mechanisms, suggesting that exogenous application of NO could be a potential tool to evolve cold tolerance as well as climate resilience in rice.

scholarly journals Insights into nitric oxide-mediated water balance, antioxidant defence and mineral homeostasis in rice (Oryza sativa L.) under chilling stress

Nitric Oxide ◽  
2020 ◽  
Vol 100-101 ◽  
pp. 7-16 ◽  
Author(s):  
Abdullah Al Mamun Sohag ◽  
Md Tahjib-Ul-Arif ◽  
Sonya Afrin ◽  
Md Kawsar Khan ◽  
Md Abdul Hannan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Oryza Sativa ◽  
Water Balance ◽  
Oryza Sativa L ◽  
Chilling Stress ◽  
Antioxidant Defence ◽  
Mineral Homeostasis

scholarly journals Exogenous Nitric Oxide Pretreatment Enhances Chilling Tolerance of Anthurium

2018 ◽  
Vol 143 (1) ◽  
pp. 3-13 ◽  
Author(s):  
Lijian Liang ◽  
Yanming Deng ◽  
Xiaobo Sun ◽  
Xinping Jia ◽  
Jiale Su
Keyword(s):  
Nitric Oxide ◽  
Fruits And Vegetables ◽  
Chilling Stress ◽  
Chilling Injury ◽  
Photochemical Efficiency ◽  
Membrane Integrity ◽  
Antioxidant Response ◽  
Chilling Tolerance ◽  
No Donor ◽  
Cell Membrane Integrity

Nitric oxide (NO) is well known for its multifaceted physiological roles as a signaling molecule in plants. Previous studies have indicated that exogenous application of NO may be useful for alleviating chilling injury (CI) in fruits and vegetables. However, the potential role and mechanism of NO in mitigating chilling stress in anthurium (Anthurium andraeanum) remain unclear. In this study, physiological and biochemical analysis were performed to investigate the effects of exogenous NO in alleviating CI in anthurium. Anthurium seedling plants were treated with the NO donor sodium nitroprusside (SNP) at four concentrations (0, 0.2, 0.4, and 0.8 mm) and stored at 12/5 °C (day/night) for 15 day. The results showed that exogenous SNP mitigated the adverse effects of chilling on anthurium, and the most effective concentration was 0.2 mm. In addition, NO effectively improved the CI index, malondialdehyde (MDA) content, electrolyte leakage, photochemical efficiency (Fv/Fm), and chlorophyll loss of anthurium during low temperatures. Pretreatment with SNP also increased the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX); the content of antioxidants including glutathione (GSH), ascorbic acid (AsA), and phenolics; and reduced the accumulation of hydrogen peroxide and O2−. SNP pretreatment at 0.2 mm also significantly promoted the accumulation of proline, increased the activity of Δ1-pyrroline-5-carboxylate synthetase (P5CS), and reduced the activity of proline dehydrogenase (PDH), when compared with control (0 mm SNP→Chilling) under chilling stress. These results indicated that NO could enhance the chilling tolerance of anthurium by elicitation of an antioxidant response and proline accumulation for maintaining cell membrane integrity.

Abscisic Acid-Induced Chilling Tolerance in Maize Seedlings Is Mediated by Nitric Oxide and Associated with Antioxidant System

2011 ◽  
Vol 378-379 ◽  
pp. 423-427 ◽  
Author(s):  
Hai Yan Li ◽  
Wan Zhong Zhang
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Abscisic Acid ◽  
Chilling Stress ◽  
Chilling Tolerance ◽  
Maize Seedlings ◽  
No Donor ◽  
Contrary Effect

Abscisic acid (ABA) and sodium nitroprusside (SNP) treatment significantly increased chilling tolerance in maize seedlings. ABA in combination with nitric oxide (NO) donor SNP further enhanced the ABA-induced chilling tolerance. But the addition of NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO) nullified the increasing effect of SNP on chilling tolerance. In addition, the combination of ABA and PTIO decreased the ABA-induced chilling tolerance. Measurement of activities of superoxide dismutase (SOD) and catalase (CAT), hydrogen peroxide (H2O2) content and the level of lipid peroxidation (in terms of malondialdehyde) indicated that chilling stress induced an oxidative stress in maize seedlings. ABA treatment enabled maize seedlings to maintain higher SOD and CAT activities and lower level of H2O2 and lipid peroxidation under chilling stress. ABA in combination with SNP further enhanced the ABA-induced increase in SOD and CAT activities and lowered the chilling stress-induced lipid peroxidation in the ABA-treated seedlings. But the addition of PTIO scavenged the increasing effect of SNP. In addition, the combination of ABA and PTIO had a contrary effect with that of ABA and SNP. These results suggest that the ABA-induced chilling tolerance is mediated by NO, NO is involved in ABA-induced chilling tolerance by increasing activities of antioxidant enzymes and reduced endogenous H2O2 accumulation.

scholarly journals Temporal profiling of primary metabolites under chilling stress and its association with seedling chilling tolerance of rice (Oryza sativa L.)

Rice ◽  
2013 ◽  
Vol 6 (1) ◽  
pp. 23 ◽  
Author(s):  
Xiu-Qin Zhao ◽  
Wen-Sheng Wang ◽  
Fan Zhang ◽  
Ting Zhang ◽  
Wen Zhao ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Chilling Stress ◽  
Chilling Tolerance ◽  
Primary Metabolites

CO2 laser enhances the chilling tolerance of wheat seedlings by stimulating NO synthesis

2016 ◽  
Vol 96 (5) ◽  
pp. 796-807
Author(s):  
Yi-ping Chen ◽  
Qiang Liu ◽  
Dong Chen
Keyword(s):  
Nitric Oxide ◽  
Laser Irradiation ◽  
Sodium Tungstate ◽  
Chilling Stress ◽  
Chilling Tolerance ◽  
The Other ◽  
Control Group ◽  
Wheat Seedlings ◽  
Oxide Synthase ◽  
Protein Treatment

To investigate the mechanism by which laser irradiation enhances the chilling tolerance of wheat seedlings, seeds were exposed to different treatments, and biochemical parameters were measured. Compared with the control group, chilling stress (CS) led to an increase in the concentrations of malondialdehyde (MDA) and H2O2, and decreases in the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), catalase (CAT), peroxidase (POD), and nitric oxide synthase (NOS), and the concentrations of nitric oxide (NO) and protein. Treatment with 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), sodium tungstate (ST), and NG-nitro-L-arginine methyl ester (L-NAME) followed by CS resulted in further increases in the concentrations of MDA and H2O2 and further decreases in the other parameters. However, treatment with PTIO, ST, and L-NAME followed by laser irradiation had the opposite effects on these parameters. When the seeds were treated with PTIO, ST, and L-NAME followed by laser and CS, the concentrations of MDA and H2O2 were significantly lower and the other parameters were higher than in the PTIO, ST, and L-NAME plus CS groups. These results suggest that CO2 laser irradiation enhances the chilling tolerance of wheat seedlings by stimulating endogenous NO synthesis.

scholarly journals Molecular insights into the functional role of nitric oxide (NO) as a signal for plant responses in chickpea

2018 ◽  
Vol 45 (2) ◽  
pp. 267 ◽  
Author(s):  
Parankusam Santisree ◽  
Pooja Bhatnagar-Mathur ◽  
Kiran K. Sharma
Keyword(s):  
Nitric Oxide ◽  
Large Scale ◽  
Molecular Mechanisms ◽  
Lipid Transfer Protein ◽  
Positive Impact ◽  
Large Subunit ◽  
Plant Responses ◽  
Acid Oxidase ◽  
No Donor ◽  
Bisphosphate Carboxylase

The molecular mechanisms and targets of nitric oxide (NO) are not fully known in plants. Our study reports the first large-scale quantitative proteomic analysis of NO donor responsive proteins in chickpea. Dose response studies carried out using NO donors, sodium nitroprusside (SNP), diethylamine NONOate (DETA) and S-nitrosoglutathione (GSNO) in chickpea genotype ICCV1882, revealed a dose dependent positive impact on seed germination and seedling growth. SNP at 0.1 mM concentration proved to be most appropriate following confirmation using four different chickpea genotypes. while SNP treatment enhanced the percentage of germination, chlorophyll and nitrogen contents in chickpea, addition of NO scavenger, cPTIO reverted its impact under abiotic stresses. Proteome profiling revealed 172 downregulated and 76 upregulated proteins, of which majority were involved in metabolic processes (118) by virtue of their catalytic (145) and binding (106) activity. A few crucial proteins such as S-adenosylmethionine synthase, dehydroascorbate reductase, pyruvate kinase fragment, 1-aminocyclopropane-1-carboxylic acid oxidase, 1-pyrroline-5-carboxylate synthetase were less abundant whereas Bowman-Birk type protease inhibitor, non-specific lipid transfer protein, chalcone synthase, ribulose-1-5-bisphosphate carboxylase oxygenase large subunit, PSII D2 protein were highly abundant in SNP treated samples. This study highlights the protein networks for a better understanding of possible NO induced regulatory mechanisms in plants.

scholarly journals Comparison of gene co-networks reveals the molecular mechanisms of the rice (Oryza sativa L.) response to Rhizoctonia solani AG1 IA infection

2018 ◽  
Vol 18 (5) ◽  
pp. 545-557 ◽  
Author(s):  
Jinfeng Zhang ◽  
Wenjuan Zhao ◽  
Rong Fu ◽  
Chenglin Fu ◽  
Lingxia Wang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Rhizoctonia Solani ◽  
Molecular Mechanisms ◽  
Oryza Sativa L

scholarly journals Reactive Oxygen Species (ROS) Metabolism and Nitric Oxide (NO) Content in Roots and Shoots of Rice (Oryza sativa L.) Plants under Arsenic-Induced Stress

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1014 ◽  
Author(s):  
Ernestina Solórzano ◽  
Francisco J. Corpas ◽  
Salvador González-Gordo ◽  
José M. Palma
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Antioxidative Systems ◽  
Ros Metabolism ◽  
As Stress

Arsenic (As) is a highly toxic metalloid for all forms of life including plants. Rice is the main food source for different countries worldwide, although it can take up high amounts of As in comparison with other crops, showing toxic profiles such as decreases in plant growth and yield. The induction of oxidative stress is the main process underlying arsenic toxicity in plants, including rice, due to an alteration of the reactive oxygen species (ROS) metabolism. The aim of this work was to gain better knowledge on how the ROS metabolism and its interaction with nitric oxide (NO) operate under As stress conditions in rice plants. Thus, physiological and ROS-related biochemical parameters in roots and shoots from rice (Oryza sativa L.) were studied under 50 μM arsenate (AsV) stress, and the involvement of the main antioxidative systems and NO in the response of plants to those conditions was investigated. A decrease of 51% in root length and 27% in plant biomass was observed with 50 μM AsV treatment, as compared to control plants. The results of the activity of superoxide dismutase (SOD) isozymes, catalase, peroxidase (POD: total and isoenzymatic), and the enzymes of the ascorbate–glutathione cycle, besides the ascorbate and glutathione contents, showed that As accumulation provoked an overall significant increase of most of them, but with different profiles depending on the plant organ, either root or shoot. Among the seven identified POD isozymes, the induction of the POD-3 in shoots under As stress could help to maintain the hydrogen peroxide (H2O2) redox homeostasis and compensate the loss of the ascorbate peroxidase (APX) activity in both roots and shoots. Lipid peroxidation was slightly increased in roots and shoots from As-treated plants. The H2O2 and NO contents were enhanced in roots and shoots against arsenic stress. In spite of the increase of most antioxidative systems, a mild oxidative stress situation appears to be consolidated overall, since the growth parameters and those from the oxidative damage could not be totally counteracted. In these conditions, the higher levels of H2O2 and NO suggest that signaling events are simultaneously occurring in the whole plant.

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