scholarly journals Radiosensitizer Effect of β-Apopicropodophyllin Against Colorectal Cancer via Induction of Reactive Oxygen Species and Apoptosis

2021 ◽  
Author(s):  
Na-Gyeong Lee ◽  
Jin-Hee Kwon ◽  
A-Ram Kang ◽  
Jie-Young Song ◽  
Sang-Gu Hwang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Reactive Oxygen Species ◽  
Radiation Treatment ◽  
Combined Treatment ◽  
Reactive Oxygen ◽  
Cell Counting ◽  
Cancer Drug ◽  
Oxygen Species ◽  
In Vivo Animal Model ◽  
Anti Cancer

β-apopicropodophyllin (APP), a derivative of podophyllotoxin (PPT), has been identified as a potential anti-cancer drug. This study tested whether APP acts as an an-ti-cancer drug and can sensitize colorectal cancer (CRC) cells to radiation treatment. APP had an anti-cancer effect against the CRC cell lines HCT116, and DLD-1, SW480 and COLO320DM with IC50 values of 7.88 nM, and 8.22 nM, 9.84 nM and 7.757 nM, respec-tively induction of DNA damage. Colonogenic and cell counting assays indicated that the combined treatment of APP and γ-ionizing radiation (IR) showed greater retardation of cell growth than either alone, suggesting that APP sensitizes CRC cells to IR. Annexin V-propidium iodide (PI) assays and immunoblot analysis showed that the combined treatment of APP and IR increased apoptosis in CRC cells compared with either APP or IR alone. Results obtained from the xenograft experiments also indicated that the combination of APP and IR enhanced apoptosis in in vivo animal model. Apoptosis induction by the combined treatment of APP and IR resulted from reactive oxygen species (ROS). Inhibition of ROS by N-acetylcysteine (NAC) restored cell viability and decreased the induction of apoptosis by APP and IR in CRC cells. Taken together, these results indicate that a combined treatment of APP and IR might promote apoptosis by inducing ROS in CRC cells.

scholarly journals Radiosensitizer Effect of β-Apopicropodophyllin against Colorectal Cancer via Induction of Reactive Oxygen Species and Apoptosis

2021 ◽  
Vol 22 (24) ◽  
pp. 13514
Author(s):  
Jin-Hee Kwon ◽  
Na-Gyeong Lee ◽  
A-Ram Kang ◽  
Jie-Young Song ◽  
Sang-Gu Hwang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Reactive Oxygen Species ◽  
Radiation Treatment ◽  
Combined Treatment ◽  
Reactive Oxygen ◽  
Cell Counting ◽  
Cancer Drug ◽  
Oxygen Species ◽  
In Vivo Animal Model ◽  
Anti Cancer

β-apopicropodophyllin (APP), a derivative of podophyllotoxin (PPT), has been identified as a potential anti-cancer drug. This study tested whether APP acts as an anti-cancer drug and can sensitize colorectal cancer (CRC) cells to radiation treatment. APP exerted an anti-cancer effect against the CRC cell lines HCT116, DLD-1, SW480, and COLO320DM, with IC50 values of 7.88 nM, 8.22 nM, 9.84 nM, and 7.757 nM, respectively, for the induction of DNA damage. Clonogenic and cell counting assays indicated that the combined treatment of APP and γ-ionizing radiation (IR) showed greater retardation of cell growth than either treatment alone, suggesting that APP sensitized CRC cells to IR. Annexin V–propidium iodide (PI) assays and immunoblot analysis showed that the combined treatment of APP and IR increased apoptosis in CRC cells compared with either APP or IR alone. Results obtained from the xenograft experiments also indicated that the combination of APP and IR enhanced apoptosis in the in vivo animal model. Apoptosis induction by the combined treatment of APP and IR resulted from reactive oxygen species (ROS). Inhibition of ROS by N-acetylcysteine (NAC) restored cell viability and decreased the induction of apoptosis by APP and IR in CRC cells. Taken together, these results indicate that a combined treatment of APP and IR might promote apoptosis by inducing ROS in CRC cells.

scholarly journals Generation of reactive oxygen species is the primary mode of action and cause of survivin suppression by sepantronium bromide (YM155)

2021 ◽  
Author(s):  
Tasaduq Hussain Wani ◽  
Goutam Chowdhury ◽  
Anindita Chakrabarty
Keyword(s):  
Reactive Oxygen Species ◽  
Dna Damage ◽  
Mode Of Action ◽  
Reactive Oxygen ◽  
Cancer Drug ◽  
Oxygen Species ◽  
Secondary Effects ◽  
Primary Mode ◽  
Anti Cancer ◽  
Sepantronium Bromide

The anti-cancer drug YM155's primary mode of action is generation of reactive oxygen species, while survivin suppression and DNA damage are secondary effects.

scholarly journals Targeted production of reactive oxygen species in mitochondria to overcome cancer drug resistance

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Hai Wang ◽  
Zan Gao ◽  
Xuanyou Liu ◽  
Pranay Agarwal ◽  
Shuting Zhao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Drug Resistance ◽  
Reactive Oxygen ◽  
Cancer Drug ◽  
Oxygen Species ◽  
Cancer Drug Resistance

Superoxide dismutase mRNA and protein level in human colorectal cancer

2010 ◽  
Vol 5 (5) ◽  
pp. 590-599 ◽  
Author(s):  
Michał Skrzycki ◽  
Monika Majewska ◽  
Hanna Czeczot
Keyword(s):  
Colorectal Cancer ◽  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Liver Metastases ◽  
Tumor Cells ◽  
Protein Level ◽  
Colorectal Adenocarcinoma ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Colorectal Cancer Liver Metastases

AbstractImpairments of antioxidant enzyme expression are often concomitant with the onset of cancer. Due to epigenetic factors causing an inflammatory state the gastrointestinal tract can become exposed to reactive oxygen species. The purpose of our work was to evaluate mRNA and protein levels of superoxide dismutase isoenzymes in human colorectal adenocarcinoma due to its clinical advancement, and in colorectal cancer liver metastases. Evaluation of SOD expression in regard to CRC advancement, seems useful for clinical applications due to different tumor cells sensitivity to reactive oxygen species based treatment. Studies were conducted on a group of 27 patients: 15 diagnosed with colorectal adenocarcinoma and 12 diagnosed with colorectal cancer liver metastases. The mRNA level was determined by RT-PCR, and protein level by Western blotting. We observed significant (P≤0.05) changes of mRNA and protein level of SOD isoenzymes in subsequent stages of colorectal adenocarcinoma advancement and in colorectal cancer liver metastases. Differences in mRNA and protein level of SOD isoenzymes in colorectal adenocarcinoma and its liver metastases indicates that SOD participate in adaptation of tumor cells to oxidative stress, and maintain certain level of ROS, necessary for appropriate cell proliferation. Expression of superoxide dismutase isoenzymes seems to be regulated not only at transcriptional level, but also posttranscriptional.

Reactive Oxygen Species, Cancer and Anti-Cancer Therapies

2009 ◽  
Vol 3 (1) ◽  
pp. 342-366 ◽  
Author(s):  
Gina Manda ◽  
Marina T. Nechifor ◽  
Teodora-Monica Neagu
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Cancer Therapies ◽  
Oxygen Species ◽  
Anti Cancer

scholarly journals Microenvironmental Reactive Oxygen Species in Colorectal Cancer: Involved Processes and Therapeutic Opportunities

Cancers ◽  
2021 ◽  
Vol 13 (20) ◽  
pp. 5037
Author(s):  
Maria Alba Sorolla ◽  
Ivan Hidalgo ◽  
Anabel Sorolla ◽  
Robert Montal ◽  
Ona Pallisé ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Reactive Oxygen Species ◽  
Cancer Progression ◽  
Cellular Proliferation ◽  
Therapeutic Potential ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Mesenchymal Transition ◽  
Screening Programs ◽  
Systemic Treatments

Colorectal cancer (CRC) is the fourth most common cause of cancer deaths worldwide. Although screening programs have reduced mortality rates, there is a need for research focused on finding the main factors that lead primary CRC to progress and metastasize. During tumor progression, malignant cells modify their habitat, corrupting or transforming cells of different origins and creating the tumor microenvironment (TME). Cells forming the TME like macrophages, neutrophils, and fibroblasts generate reactive oxygen species (ROS) that modify the cancer niche. The effects of ROS in cancer are very diverse: they promote cellular proliferation, epithelial-to-mesenchymal transition (EMT), evasion of cell death programs, migration, and angiogenesis. Due to the multifaceted role of ROS in cancer cell survival and function, ROS-modulating agents such as antioxidants or pro-oxidants could have therapeutic potential in cancer prevention and/or as a complement to systemic treatments. In this review, we will examine the main ROS producer cells and their effects on cancer progression and metastasis. Furthermore, we will enumerate the latest clinical trials where pro-oxidants and antioxidants have therapeutic uses in CRC.

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