scholarly journals Chitosan/alginate Hydrogel Dressing Loaded FGF/VE-cadherin: To Accelerate Full-thickness Skin Regeneration and More Normal Skin Repairs

2021 ◽  
Author(s):  
Lai Wei ◽  
Jianying Tan ◽  
Li Li ◽  
Sainan Liu ◽  
Huanran Wang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rheological Properties ◽  
Normal Skin ◽  
Healing Process ◽  
Antibacterial Properties ◽  
Hair Follicles ◽  
Skin Regeneration ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

The process of full-thickness skin regeneration is complex and has many parameters involved, which makes it difficult to use a single dressing to meet the various requirements of the complete regeneration at the same time. Therefore, developing hydrogel dressings with multifunction, including tunable rheological properties and aperture, hemostatic, antibacterial and super cytocompatibility, is a desirable candidate in wound healing. In this study, a series of complex hydrogels were developed via the hydrogen bond and covalent bond between chitosan (CS) and alginate (SA). These hydrogels exhibited suitable pore size and tunable rheological properties for cell adhesion. Chitosan endowed hemostatic, antibacterial properties and great cytocompatibility and thus solved two primary problems in the early stage of the wound healing process. Moreover, the sustained cytocompatibility of the hydrogels was further investigated after adding FGF and VE-cadherin via the co-culture of L929 and EC for 12 days. The confocal 3D fluorescent images showed that the cells were spherical and tended to form multicellular spheroids, which distributed in about 40-60μm thick hydrogels. Furthermore, the hydrogel dressings significantly accelerate defected skin turn to normal skin with proper epithelial thickness and new blood vessels and hair follicles through the histological analysis of in vivo wound healing. The findings mentioned above demonstrated that the CS/SA hydrogels with growth factors have tremendous potential as multifunctional hydrogel dressings for full-thickness skin regeneration incorporated with hemostatic, antibacterial, sustained cytocompatibility for 3D cell culture and normal skin repairing.

scholarly journals Wound healing in immunocompromised dogs: A comparison between the healing effects of moist exposed burn ointment and honey

2020 ◽  
Vol 13 (12) ◽  
pp. 2793-2797
Author(s):  
Musa Alshehabat ◽  
Wael Hananeh ◽  
Zuhair Bani Ismail ◽  
Safwan Abu Rmilah ◽  
Mahmoud Abu Abeeleh
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Analysis Data ◽  
Punch Biopsy ◽  
Histopathological Analysis ◽  
Full Thickness ◽  
Imaging Data ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Skin Punch Biopsy

Background and Aim: Natural topical products have been used to enhance wound healing, especially in immunocompromised animals. The aims of this study were to evaluate and to compare the effects of moist exposed burn ointment (MEBO) and honey on the healing of full-thickness skin wounds in immunocompromised dogs. Materials and Methods: The study was conducted using 12 adults, apparently healthy mongrel dogs. Immunosuppression was induced in six dogs by oral administration of prednisone (2 mg/kg) and azathioprine (2 mg/kg), once a day for 21 days. On each dog, a total of 9, 1.5 cm in diameter full-thickness skin circular wounds were created aseptically in the thoracolumbar area under general anesthesia using customized skin punch biopsy kit. In a random fashion, three wounds in each dog were treatment using MEBO (contains b-sitosterol, baicalin, and berberine as active ingredients in a base of beeswax and sesame oil), honey or no treatment (control), once per day for 21 days. Wounds were grossly evaluated once a day for signs of inflammation or infection. In addition, biopsy specimens and digital imaging data of each wound were obtained on days 7, 14, and 21 for histopathological evaluation of the healing process. Results: Wounds in immunocompromised dogs appeared to heal significantly in a slower fashion than in non-immunocompromised counterparts. Digital analysis data showed that MEBO-treated wounds expressed better epithelialization area, faster contraction, and smaller wound area percentage when compared with honey-treated wounds. Histopathological analysis showed significantly higher angiogenesis scores in MEBO-treated wounds when compared with other treatments. Conclusion: Results of this study showed that MEBO resulted in significant enhancement of wound healing in both healthy and immunocompromised dogs. However, when compared to honey, the wound healing effect of MEBO was superior to that of honey.

scholarly journals Multi-faceted enhancement of full-thickness skin wound healing by treatment with autologous micro skin tissue columns

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Christiane Fuchs ◽  
Linh Pham ◽  
Jermaine Henderson ◽  
Katherine J. Stalnaker ◽  
R. Rox Anderson ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Donor Site ◽  
Skin Grafting ◽  
Donor Site Morbidity ◽  
Skin Tissue ◽  
Full Thickness ◽  
Impaired Wound Healing ◽  
Full Thickness Skin ◽  
Thickness Skin

AbstractImpaired wound healing is an immense medical challenge, and while autologous skin grafting remains the “gold-standard” therapeutic option for repairing wounds that cannot be closed by primary or secondary intention, it is limited by substantial donor site morbidity. We previously developed the alternative approach of harvesting full-thickness skin tissue in the form of “micro skin tissue columns” (MSTCs), without causing scarring or any other long-term morbidity. In this study we investigated how MSTC treatment affects the different cellular processes involved in wound healing. We found that MSTC-derived cells were able to remodel and repopulate the wound volume, and positively impact multiple aspects of the wound healing process, including accelerating re-epithelialization by providing multiple cell sources throughout the wound area, increasing collagen deposition, enhancing dermal remodeling, and attenuating the inflammatory response. These effects combined to enhance both epidermal and dermal wound healing. This MSTC treatment approach was designed for practical clinical use, could convey many benefits of autologous skin grafting, and avoids the major drawback of donor site morbidity.

scholarly journals THE DYNAMICS OF WOUND HEALING IN THE TREATMENT WITH THE OINTMENT WITH A DRY EXTRACT OF POTENTILLA

2018 ◽  
Vol 25 (6) ◽  
pp. 105-109
Author(s):  
L. P. Lezhnevа ◽  
A. M. Temirbulatova ◽  
E. F. Stepanova ◽  
D. V. Veselova
Keyword(s):  
Wound Healing ◽  
Dosage Form ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Skin Wounds ◽  
Full Thickness ◽  
Dry Extract ◽  
Full Thickness Skin ◽  
Thickness Skin

Aim. The study was designed for the evaluation of the effect of ointments with dry extracts of potentilla on the dynamics of the healing process of full-thickness skin wounds.Materials and methods. The wound healing activity estimation of the proposed dosage form was conducted on white Wistar rats. The results of macroscopic studies (the area of the wound defect, the state of the scab, the presence of hemorrhage beneath it, the degree of inflammatory processes in the underlying tissues, including deeply located tissue structures, up to the muscle elements) and the results of histological examination were the criterion for evaluating the effectiveness of the wound healing agent. In the course of the experiment, the animals were divided into 4 groups: the main group (treatment with the ointment with a dry extract of potentilla), control group No. 1 (treatment with the ointment base), control group No. 2 (without treatment), and control group No. 3 (treatment with the «Chlorophyllipt» drug.Results. The results of the macroscopic and histological studies made it possible to conclude that the wound healing process in the treatment with the ointment with a dry extract of potentilla had a number of features. The proposed dosage form enhanced the regeneration of the multilayered squamous epithelium, accelerated the maturation of the granulation tissue, and eliminated the inflammation and infiltration in the first days of treatment, which led to a significant reduction in the healing time when it was applied (15-18 days). In the animals of the control groups No. 1 and No. 2, the described processes occurred much more slowly, and inflammatory infiltration persisted almost throughout the entire experiment. The wound surface was healed on the 27-30th day. A lot of common features were noted in the mechanism and timing of wound healing in the treatment with the ointment with a dry extract of potentilla and a reference preparation – «Сhlorophyllipt». Conclusion. The ointment with a dry extract of potentilla significantly speeds up the healing process of full-thickness skin wounds.

Sericin hydrogels promote skin wound healing with effective regeneration of hair follicles and sebaceous glands after complete loss of epidermis and dermis

2018 ◽  
Vol 6 (11) ◽  
pp. 2859-2870 ◽  
Author(s):  
Chao Qi ◽  
Luming Xu ◽  
Yan Deng ◽  
Guobin Wang ◽  
Zheng Wang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Hair Follicles ◽  
Complete Loss ◽  
Full Thickness ◽  
Sebaceous Glands ◽  
Skin Injury ◽  
Skin Wound Healing ◽  
Full Thickness Skin ◽  
Thickness Skin

Treating full-thickness skin injury with photo-crosslinkable sericin hydrogel for scarless regeneration with effective restoration of skin appendages.

scholarly journals The Effectivity of Ozonated VCO on Healing Full Grafted Skin graft Study on Progenitor Cell Endothelial Proliferation and Angiogenesis in Sprague Dawley Mice

2021 ◽  
Vol 8 (2) ◽  
pp. 133-138
Author(s):  
Rollin Hansen Sidauruk ◽  
Renny Yuniati ◽  
Djoko Handojo
Keyword(s):  
Wound Healing ◽  
Skin Graft ◽  
Healing Process ◽  
Wound Healing Process ◽  
Full Thickness ◽  
Sprague Dawley ◽  
Full Thickness Skin Graft ◽  
Accelerate Wound Healing ◽  
Full Thickness Skin ◽  
Thickness Skin

Abstract   Background : Full thickness  Skin graft is one technique to accelerate wound healing. EPC proliferation and new blood vessel formation have an important role in the success of Ful thickness  Skin graft. Ozone content in Ozonated VCO  has the ability to increase growth factors so that it can accelerate wound healing   Aims : This study was aimed to prove the effects of ozonated vco  in various doses in increasing EPC proliferation and angiogenesis in full thickness skin graft wounds.   Methods : This research is an experimental study with randomized post test only with parallel group design. 40 Sprague Dawley rats performed autologous  Skin grafts at the same time. Samples were randomly divided into 8 groups, two control groups (X1 and X5), and 6 other groups were given a multi-dose ozonated oil  50.6 mg / ml (X2, X6), 101.3 mg / ml (X3, X7) , 202.6 mg / ml (X4, X8). Conduct termination on day 6 and day 12. Examination of the number of  EPC proliferation and the number of angiogenesis in each group. Data were analyzed by Saphiro Wijk, ANOVA, post hoc benferonni and pearsons   Results : The number of EPC proliferation in groups X1, X2, X3, X4, X5, X6, X7, X8 has a mean of 6.2 + 0.837, 10 + 1.581, 13.80 + 2.049, 14.80+ 2,280, 9.40 + 2,074 , 13.20 + 1,483, 19,0 + 2,055 and 19.20 + 1,095.The number of Angiogenesis in groups X5, X6, X7, X8 has a mean of 9.20 + 1,095,14,60 + 2,881,17,40+ 3,286, 18,80 + 2,168,17,40 + 3,286,18.80+ 2,168. The administration of ozonated VCO  dose of 202.6 mg / ml gave the best results in the amount of angiogenesis (p = 0.001) and the amount of angiogenesis (p=0,001).Correlation analysis between EPC proliferation and angiogenesis showed a significant correlation (p = 0.001 and r = 0.738).   Conclusion : Ozonated oil can increase the number of EPC proliferation and angiogenesis in the wound healng process of Full thickness  Skin graft. The ozone dose of 202,6 mg / ml showed the best effect on increase wound healing process. Keyword : Full thickness skin graft, Ozonated oil , EPC, Angiogenesis  

scholarly journals Development of a Novel Pre-Vascularized Three-Dimensional Skin Substitute Using Blood Plasma Gel

2018 ◽  
Vol 27 (10) ◽  
pp. 1535-1547 ◽  
Author(s):  
Niann-Tzyy Dai ◽  
Wen-Shyan Huang ◽  
Fang-Wei Chang ◽  
Lin-Gwei Wei ◽  
Tai-Chun Huang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Plasma ◽  
Three Dimensional ◽  
Skin Substitute ◽  
Strength Analysis ◽  
Full Thickness ◽  
Skin Substitutes ◽  
Full Thickness Skin ◽  
Post Surgery ◽  
Thickness Skin

Skin substitutes with existing vascularization are in great demand for the repair of full-thickness skin defects. In the present study, we hypothesized that a pre-vascularized skin substitute can potentially promote wound healing. Novel three-dimensional (3D) skin substitutes were prepared by seeding a mixture of human endothelial progenitor cells (EPCs) and fibroblasts into a human plasma/calcium chloride formed gel scaffold, and seeding keratinocytes onto the surface of the plasma gel. The capacity of the EPCs to differentiate into a vascular-like tubular structure was evaluated using immunohistochemistry analysis and WST-8 assay. Experimental studies in mouse full-thickness skin wound models showed that the pre-vascularized gel scaffold significantly accelerated wound healing 7 days after surgery, and resembled normal skin structures after 14 days post-surgery. Histological analysis revealed that pre-vascularized gel scaffolds were well integrated in the host skin, resulting in the vascularization of both the epidermis and dermis in the wound area. Moreover, mechanical strength analysis demonstrated that the healed wound following the implantation of the pre-vascularized gel scaffolds exhibited good tensile strength. Taken together, this novel pre-vascularized human plasma gel scaffold has great potential in skin tissue engineering.

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