Biological properties and molecular and genetic characteristics of plague microbe strains circulating in sandy natural plague foci of the Republic of Kazakhstan

Bacteriology ◽  
2020 ◽  
Vol 5 (3) ◽  
pp. 25-33
Author(s):  
Z.Zh. Abdel ◽  
◽  
Т.V. Меkа-Меchеnkо ◽  
А.А. Аbdirasilova ◽  
R.S. Musagaliyeva ◽  
...  
Keyword(s):  
Molecular Genetic ◽  
Biological Properties ◽  
Genetic Characteristics ◽  
Central Asian ◽  
Genetic Features ◽  
Natural Foci ◽  
Parasitic System ◽  
The Republic ◽  
Main Component ◽  
Atypical Strains

Since 2010, an active course of epizootics with the release of the plague pathogen, isolated from hosts and vectors has been established in 8 autonomous foci of the plague from 14 autonomous foci of the Central Asian plague focus in Kazakhstan. It was necessary to take into account the parameters of variability of the main component of the parasitic system – the plague microbe in the process of certification of landscape and epizootological zoning of natural foci of plague in Kazakhstan. The aim of the work was to study the phenotypic and genetic properties of strains of the plague microbe isolated in natural sandy plague foci of Kazakhstan. Materials and methods. The work used 1196 strains of Yersinia pestis isolated over the past 10 years (2010–2019) from natural sandy plague foci, strain passports, literature sources, data on certification of plague foci in Kazakhstan. The study of the strains was carried out by bacteriological, serological and molecular genetic methods. Results. Certification and typification of the territories of sandy plague foci were carried out, taking into account the phenotypic and molecular-genetic properties of Y. pestis strains isolated from 12 autonomous foci of the Central Asian plague focus of Kazakhstan in 2010–2019. According to the results of the study, 84 atypical strains were identified. As a result of the analysis, 18 genotypes were identified among the studied strains, of which 13 (72.2%) were unique and did not repeat in the sample. The remaining 5 genotypes formed 5 clusters, combining 20 strains (60.6%) and all strains were phylogenetically assigned to representatives of the Mediaevalis biovar. Key words: plague microbe, plague foci, phenotypic features, molecular genetic features

scholarly journals Biological Properties and Genetic Characteristics of Francisella tularensis Strains Isolated in the Territory of the Rostov Region in 2020

2021 ◽  
pp. 134-140
Author(s):  
M. V. Tsimbalistova ◽  
V. M. Sorokin ◽  
N. V. Aronova ◽  
A. S. Anisimova ◽  
N. L. Pichurina ◽  
...  
Keyword(s):  
Francisella Tularensis ◽  
Molecular Genetic ◽  
Biological Properties ◽  
Snp Markers ◽  
Pcr Analysis ◽  
Snp Analysis ◽  
Genetic Characteristics ◽  
Tularensis Subsp ◽  
Rostov Region ◽  
Natural Foci

Objective of the study was to investigate biological properties and genetic characteristics of tularemia agent strains isolated from natural foci of the Rostov Region in 2020.Materials and methods. Field material from natural foci of the Rostov Region was examined by serological, bacteriological, biological, and molecular-genetic methods. Cultural-morphological, biochemical, antigenic and pathogenic properties of isolated cultures were studied. Protein profles were obtained through MALDI-TOF MS using mass spectrometer Autoflex speed III Bruker Daltonics and Flex Control of Biotyper software. The genetic characteristics of the strains were determined by VNTR and INDEL typing and SNP analysis.Results and discussion. Six strains of tularemia pathogen were isolated from mouse-like rodents using biological method. The investigation of their biological features and data of PCR analysis and INDEL typing with canonical markers showed that all strains are typical representatives of the Francisella tularensis subsp. holarctica biovar EryR. VNTR typing by six genetic loci revealed that all strains belong to four individual genotypes. The strain isolated in 2020 in the Salsky district was identical to the strain which was isolated in the same area in 1989. Based on the whole genome sequencing of two strains, we established that they are closest to the cultures isolated in Turkey (2009, 2012) and Khanty-Mansiysk (2013) by the studied set of SNP markers. Thus, we found that both identical (or closely related) clones of the tularemia agent and new strains with unique genotypes which previously were not described for the Rostov Region can circulate in natural foci of this region for a long period of time.

scholarly journals The influence of seasonal influenza viruses biological features on the effectiveness of development strains for live influenza vaccine

2019 ◽  
pp. 24-34
Author(s):  
N. V. Larionova ◽  
I. V. Kiseleva ◽  
E. A. Bazhenova ◽  
E. P. Grigorieva ◽  
L. G. Rudenko
Keyword(s):  
Influenza Vaccine ◽  
Vaccine Development ◽  
Molecular Genetic ◽  
Biological Properties ◽  
Influenza Viruses ◽  
Biological Characteristics ◽  
Phenotypic Properties ◽  
Hyperimmune Serum ◽  
Genetic Characteristics ◽  
Effective Development

Aim. Evaluation of the efficiency of the method of reassortant strains for live influenza vaccine development and ways to optimize it, taking into account the differences in the current epidemic influenza viruses by key biological characteristics.Materials and methods. Influenza viruses — candidates for seasonal LAIVs, MDVs for Russian LAIVs A/Leningrad/134/17/57 (H2N2) and B/USSR/60/69. The vaccine strains development in developing chicken embryos included reassortment, selective passages at low temperature in the presence of hyperimmune serum to the MDV, several stages of reassortants cloning, their virological and molecular genetic characteristics. Epidemic influenza viruses and LAIVs strains were evaluated by their ability to reproduction at temperatures beyond optimal values, by sensitivity to serum inhibitors.Results. The assessment of phenotypic properties used in reassortment epidemic viruses is carried out. Presented the data on the efficiency of development reassortant strains for LAIV depending on the biological properties of circulating epidemic influenza viruses: their temperature-resistant, cold-sensitive phenotype, inhibitor resistance, and receptor specificity.Conclusion. Based on the assessment of the influence of the biological characteristics of the epidemic viruses, the rational methodological techniques for the most effective development of reassortants for LAIV are selected.

scholarly journals MOLECULAR-GENETIC CHARACTERISTICS OF SHIGELLA SONNEI-2013 STRAIN ISOLATED DURING THE OUTBREAK IN DYSENTERY IN THE REPUBLIC ABKHAZIA IN 2013

2018 ◽  
pp. 72-76
Author(s):  
O. V. Vasileva ◽  
A. S. Volynkina ◽  
I. V. Kuznetsova ◽  
S. V. Pisarenko ◽  
A. N. Kulichenko
Keyword(s):  
Nucleotide Sequence ◽  
Molecular Genetic ◽  
Housekeeping Genes ◽  
Shigella Sonnei ◽  
Personal Genome ◽  
Virulence Plasmid ◽  
Genetic Characteristics ◽  
E Coli ◽  
Type Iv ◽  
The Republic

Aim. Study of molecular-genetic properties of Shigella sonnei-2013 strain isolated during the outbreak in dysentery in the republic Abkhazia in 2013. Materials and methods. Genetic typing of the tested strains using multilocus sequence typing (MLST). Analyzed of nucleotide sequence fragments 7 of conservative «housekeeping» genes adk, fumC, icd, mdh, purA, recA, gyrB. Sequenced of DNA fragments compared with reference sequences from database of Escherichia coli MLST. Phylogenetic analysis was performed using UPGMA method and computer program START 2. Whole-genome sequencing performed on a genetic analyzer Ion Torrent Personal Genome Machine (PGM™) using fragment libraries (shot-gun). Aligning reads have been carried out with the program GS Reference Mapper. Results. Defined sequence - type of the studied strain - ST-152, one of the most common genotypes for S. sonnei. Demonstrated the high degree of similarity obtained contig to the sequences of the chromosome and plasmids А, В, С и E strains S. sonnei 53G and S. sonnei Ss046. Identified contigs with a high percentage similarity to the sequence of virulence plasmid p026-Vir of E. coli 026:H11 (H30). In the genomic S. sonnei-2013 revealed nucleotide sequence of 136 genes were found located on the p026-Vir strain of E. coli 026:Н11 (НЗО). Discovered genes controlling biosynthesis of type IV pili involved in adhesion to abiotic surfaces and biofilm formation. Conclusion. Identified structural peculiarities of strain induced by fragments of virulence plasmid p026-Vir strain E. coli 026:H 11 (H30).

scholarly journals INFLUENCE OF BLOOD GROUPS, CAST AND BLG GENES ON PRODUCTIVITY OF THE SHEEP AND GOATS OF THE ALTAY REPUBLIC

2018 ◽  
Vol 48 (4) ◽  
pp. 63-71
Author(s):  
G. M. Goncharenko ◽  
N. V. Grishina ◽  
T. S. Khoroshilova ◽  
I. V. Romanchuk ◽  
T. B. Kargachakova ◽  
...  
Keyword(s):  
Genetic Similarity ◽  
Molecular Genetic ◽  
Live Weight ◽  
Molecular Genetic Analysis ◽  
Pcr Analysis ◽  
Dna Diagnosis ◽  
Genetic Characteristics ◽  
High Productivity ◽  
The Republic ◽  
Cast Gene

The use of genetic markers in addition to traditional methods of animal selection with desirable genotypes allows to increase the share of animals with high productivity in the next generations and ensures improvement of breeding efficiency. Genetic features of the Prikatun type of the Gorno-Altay semi-fine wool breed of sheep and the white downy goat breed in the Republic of Altay were studied by the method of immunogenetic and molecular genetic analysis. The frequency of antigenic factors was identified and the index of genetic similarity between goats and sheep and their separate herds was calculated. Gene polymorphism of β-lactoglobulin (BLG) and calpastatin (CAST) was revealed by the method of DNA diagnosis. Population and genetic characteristics of the herds was studied by the genes specified. Associative genotype relation to productivity and quality of the produce obtained was analyzed. The index of genetic similarity between the goats and the sheep was at the level of 0.713, between the separate herds of the goats the index was 0.861. The ratio of genotypes in the BLG gene determined by PCR analysis in the white downy goats was S1S1– 16.1%; S1S2– 50.6%; S2S2– 33.3%. In the Prikatun type two genotypes were identified in this gene: BB with the frequency of 59.2%, and AB – 40.8%. Two different alleles were identified in the CAST gene of sheep (M and N). The genotype MM was the predominant variant in the CAST sheep gene, whose frequency was 88%. The frequency of occurrence of animals with NN genotype was 1%, MN – 11%. It was shown that the gene equilibrium in the herds was not broken, χ2= 0.931. It was noted that heterozygous goats (S1S2) by BLG gene had a higher live weight by 0.30-0.61 kg compared to other variants of BLG gene (p<0.05). It was also found that lambs with genotype MM of the CAST gene had a higher live weight by 5.5 kg than MN heterozygotes (p< 0.01). However, this difference was not revealed in other age and sex groups of animals.

Clinical and molecular genetic description of a new type of mucopolysaccharidosis in Yakutia

2021 ◽  
pp. 33-40
Author(s):  
С.Н. Новгородова ◽  
Е.Е. Гуринова ◽  
А.Л. Сухомясова ◽  
В.М. Аргунова ◽  
Л.Н. Николаева ◽  
...  
Keyword(s):  
Molecular Genetic ◽  
Clinical Signs ◽  
Autosomal Recessive Inheritance ◽  
Hereditary Disease ◽  
Hereditary Diseases ◽  
Genetic Characteristics ◽  
Lysosomal Diseases ◽  
Turkish Family ◽  
New Type ◽  
The Republic

Введение. В статье приведены клинические и молекулярно-генетические характеристики нового наследственного заболевания с аутосомно-рецессивным типом наследования - мукополисахаридоз-плюс синдрома, впервые описанного в якутской популяции и в одной турецкой семье. Синдром относится к группе лизосомных заболеваний и был внесен в международную базу наследственных заболеваний OMIM под номером # 617303. Цель: дать клиническую и молекулярно-генетическую характеристику мукополисахаридоз-плюс синдрома у пациентов, наблюдавшихся в Якутии с 2006 по 2020 год. Методы. Объектом исследования послужили клинические данные из генетических карт 17 пациентов из 15 якутских семей, наблюдавшихся и состоявших в Республиканском генетическом регистре наследственной и врожденной патологии Медико-генетического центра «Республиканской больницы №1 - Национального центра медицины» в городе Якутске. Результаты. Клиническая картина у пациентов проявляется типичными признаками мукополисахаридозов, но с более тяжелым течением заболевания, приводящим к ранней младенческой смерти. У всех больных обнаружена мутация p.R498W в гене VPS33A в гомозиготном состоянии, у их родителей - в гетерозиготном. Заключение. Характерным признаком мукополисахаридоз-плюс синдрома является ранняя манифестация, быстропрогрессирующее течение с мультисистемным поражением легких, почек, сердца, центральной нервной и гемопоэтической систем и младенческая смертность. Background. This article provides data on the clinical and molecular genetic description of a new hereditary disease with an autosomal recessive inheritance - mucopolysaccharidosis-plus syndrome, first described in the Yakut population and in one Turkish family. The syndrome belongs to the group of lysosomal diseases and was included into the international database of hereditary diseases OMIM under the number # 617303. Aim. To describe clinical and molecular genetic characteristics of mucopolysaccharidosis-plus syndrome in patients observed in Yakutia from 2006 to 2020. Methods. The material for the study was clinical data from genetic records of 17 patients from 15 Yakut families, who were observed and registered in the «Republican genetic register of hereditary and congenital pathology of the Medical Genetic Center of the Republic hospital No. 1 - National Center of Medicine» in Yakutsk city. Results. The clinical phenotype is manifested by typical clinical signs of mucopolysaccharidosis, but with a more severe course of the disease, leading to early infant death of patients. Mucopolysaccharidosis-plus syndrome cannot be diagnosed enzymatically. All patients were found to have a specific p.R498W mutation in the VPS33A gene in a homozygous state, and in their parents in a heterozygous state. Conclusions. A characteristic sign of mucopolysaccharidosis plus syndrome is early manifestation and infant mortality, as well as multisystem damage to organs: lungs, kidneys, heart, central nervous and hemopoietic systems.

OCCURRENCE AND MOLECULAR GENETIC CHARACTERISTICS OF GRAPEVINE PINOT GRIS VIRUS IN RUSSIA

2021 ◽  
Vol 1 (19) ◽  
pp. 257-259
Author(s):  
D. Shvets ◽  
S. Vinogradova
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Molecular Genetic ◽  
Genetic Characteristics ◽  
Percentage Similarity ◽  
Grapevine Pinot Gris Virus ◽  
The Republic ◽  
First Time

For the first time in Russia Grapevine Pinot gris virus (GPGV) was detected on the vineyards of the Stavropol Territory and the Republic of Crimea. It has been discovered by real-time PCR that the distribution of GPGV in the vineyards of the Krasnodar Territory is 82%, in the Stavropol Territory - 94% and in the Republic of Crimea - 68%. Nucleotide percentage similarity of the Russian GPGV isolates in comparison with the closest isolates from Genbank was 97,5-99,5%.

scholarly journals Epidemiology and molecular genetic characteristics of Lyme borreliosis pathogens circulating in tick’s population in the Almaty oblast of the Republic of Kazakhstan

2021 ◽  
Vol 97 (6) ◽  
pp. 535-545
Author(s):  
A. O. Bissenbay ◽  
A. V. Zhigailov ◽  
Yu. V. Perfilyeva ◽  
D. A. Naizabayeva ◽  
A. S. Neupokoyeva ◽  
...  
Keyword(s):  
Lyme Borreliosis ◽  
Molecular Genetic ◽  
Clinical Manifestations ◽  
Epidemiological Data ◽  
Rrna Gene ◽  
Ixodes Persulcatus ◽  
Genetic Characteristics ◽  
Haemaphysalis Punctata ◽  
The Republic ◽  
Dna Genotyping

Background. Information on the geographical distribution of different species of the Borrelia burgdorferi sensu lato (B. burgdorferi s.l.) complex is of great epidemiological importance, since different genospecies are associated with certain clinical manifestations of Lyme borreliosis. Although Almaty region of the Republic of Kazakhstan is considered to be endemic for tick-borne borreliosis, there is still no accurate data on the level of borrelia infection in ticks in the region, including information on the genotypes of circulating borrelia.The aim of this work was to study ticks collected from humans in the Almaty region of the Republic of Kazakhstan in 2018.Materials and methods. Ticks were tested for the presence of B. burgdorferi s.l. DNA, genotyping of the identified borrelia was done by sequencing of the fragment of 16S rRNA gene. The analysis of epidemiological data on the incidence of Lyme borreliosis in the Almaty region in 2013–2018 was performed.Results. Rhipicephalus turanicus (116/253), Haemaphysalis punctata (74/253), Dermacentor marginatus (28/253), and Ixodes persulcatus (23/253) were the predominant species of ticks taken from humans. The prevalence of B. burgdorferi s.l. infection in I. persulcatus ticks was 39.13% (9/23) It should be noted that the DNA of B. burgdorferi s.l. was also detected in single individuals of D. marginatus, H. punctata, and R. turanicus, although these species are not considered as competent B. burgdorferi s.l. vectors.Conclusion. As a result of sequencing of the positive homogenates of ticks, two genotypes of B. burgdorferi s.l. were identified: B. afzelii and B. garinii and/or B. bavariensis. Thus, at least two genospecies, B. afzelii and B. garinii and/or B. bavariensis, circulate in the territory of the Almaty region.

scholarly journals Epidemiological Features of Pandemic Influenza in Kyrgyzstan due to Influenza A(H1N1)pdm

2016 ◽  
Vol 15 (5) ◽  
pp. 30-37
Author(s):  
K. T. Kasymbekova ◽  
Z. S. Nurmatov ◽  
A. B. Komissarov ◽  
A. V. Fadeev ◽  
D. V. Pereyaslov ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Molecular Genetic ◽  
Influenza Viruses ◽  
Kyrgyz Republic ◽  
Genetic Characteristics ◽  
Influenza A H1n1 ◽  
The Republic ◽  
Reference Strains

Relevance. Virological laboratory of the Department of prevention of diseases and Sanitary Inspection Healthcare Ministry f of the Kyrgyz Republic is nominated as the National Centre for Influenza Surveillance and accreditated by WHO, and in 2009 is included into a global network of influenza Goal. The purpose of this study - Assessment of epidemic features of manifestations of pandemic influenza A (H1N1) pdm09 in 2009 on the territory of the Kyrgyz Republic, as a comparative study of the molecular and genetic characteristics of influenza A virus (H1N1) pdm09, circulating on the territory of the Republic and the influenza virus reference strains recommended WHO for inclusion in the vaccine for the northern hemisphere. Materials and methods. On the basis of long-term retrospective analysis of the incidence of influenza and severe acute respiratory infections (SARI), and sentinel epidemiological surveillance (SS) data studied pandemic especially in Kyrgyzstan due to influenza A(H1N1)pdm09. Comparative molecular genetic characteristics of influenza viruses A(H1N1)pdm09 allocated in Kyrgyzstan, with reference strains recommended by the World Health Organization (WHO) for inclusion in the vaccine. Results. Overall, the analysis of the results of laboratory tests carried out as part of the routine and sentinel surveillance showed that from January 2009 to March 2010 at 38.8% of those surveyed from among, influenza viruses (at 655 out of 1687) were found. From January to April 2009 in the main circulating influenza A virus (H3N2), which accounted for 55.9 - 77.9% of the number of positive findings. Seasonal influenza A virus (H1N1) was detected in January - February (35.3 and 13.4%, respectively). The share of the flu virus in January accounted for 8%, in March-April - 25%. Not typeable influenza A virus was detected in February in 8.7% of cases. Conclusions. The results of the molecular genetic and virological studies have shown that the influenza pandemic in 2009 in the Republic was due to the spread of pandemic influenza virus A (H1N1) pdm09.

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