scholarly journals Authentication of commercially important tuna species landed in Tuticorin coast of Tamil Nadu, India by SE-AFLP method

2017 ◽  
Vol 64 ◽  
Author(s):  
G. Jeyasekaran ◽  
G. Arunkumar ◽  
P. Senthil Kumar ◽  
R. Jeya Shakila ◽  
D. Sukumar
Keyword(s):  
Tamil Nadu ◽  
Thunnus Albacares ◽  
Katsuwonus Pelamis ◽  
Closely Related Species ◽  
Important Species ◽  
Fish Meat ◽  
Thunnus Obesus ◽  
Commercially Important ◽  
Euthynnus Affinis ◽  
Species Specific

Seafood serves as a valuable protein source for human population. Among the seafood, tuna is considered as one of the commercially important species worldwide. Five tuna species namely Euthynnus affinis, Auxis thazard, Katsuwonus pelamis, Thunnus albacares and Thunnus obesus are commonly landed in the Tuticorin coast of Tamil Nadu. Among the five species, the exportable quality and grade of the meat is as follows: T. albacares> T. obesus> K. pelamis> E. affinis> A. thazard. In recent years, mislabeling is done by replacing high value tuna meat with low value tuna meat or with other low value fish meat to earn illegally. Usually, the species identification is done based on the morphological features, but this cannot be applied for the processed fish. PCR based identification methods have gained importance in the identification of fish species. PCR-AFLP is one of the molecular based methods, which can differentiate even closely related species. In this study, SE-AFLP method was employed to differentiate the above five tuna species viz., E. affinis, A. thazard, K. pelamis, T. albacares and T. obesus. Species specific AFLP marker was obtained in the primer combination of EcoR1 for fresh tuna. Band Sharing Index (BSI) analysis was also performed to find the similarities and variation among the five tuna species. AFLP profile of unknown tuna products was compared with the standard AFLP profile and the tuna species authentication was done by analysing BSI score.

scholarly journals Length–weight analysis of ten species (Actinopterygii) supporting subsistence fishery in Lakshadweep waters, southern Arabian Sea

2021 ◽  
Vol 51 (3) ◽  
pp. 257-261
Author(s):  
Pentam Veli Pura Shahul Hameed ◽  
Aliyyathumada Ishyyapura Muhsin ◽  
Pathummathada Pookoya ◽  
Kutty Ranjeet
Keyword(s):  
Fish Species ◽  
Biological Information ◽  
Thunnus Albacares ◽  
Katsuwonus Pelamis ◽  
Positive Allometry ◽  
Reported Study ◽  
Weight Analysis ◽  
Total Fish ◽  
Commercially Important ◽  
Euthynnus Affinis

The length–weight relations of ten fish species representing eight genera and four families and that formed the backbone of the subsistence fishery in the Lakshadweep islands were estimated. These fishes which included four species of tuna [Katsuwonus pelamis (Linnaeus, 1758); Thunnus albacares (Bonnaterre, 1788); Auxis thazard (Lacepède, 1800); Euthynnus affinis (Cantor, 1849)], three species of needlefishes[Ablennes hians (Valenciennes, 1846); Tylosurus crocodilus (Péron et Leseur, 1821); Tylosurus acus melanotus (Bleeker, 1850)], two species of bait fishes [Spratelloides delicatulus (Bennett, 1832); Spratelloides gracilis (Temminck et Schlegel, 1846)], and one species of halfbeak (Hemiramphus archipelagicus Collette et Parin, 1978) contributed to 96% of the total fish landings. The samples were collected from fish landing centers of ten inhabited islands of Lakshadweep from 2015 to 2017. Katsuwonus pelamis showed isometric growth, S. delicatulus and S. gracilis exhibited positive allometry, while negative allometric growth was seen in other species. The coefficient a of the LWR ranged from 0.001 (A. hians) to 0.035 (T. albacares), while b ranged from 2.7 (T. acus melanotus) to 3.4 (S. delicatulus). The results of the presently reported study provide useful biological information on the stock of ten commercially important pelagic fish species supporting the traditional fisheries in Lakshadweep waters.

scholarly journals Length–weight analysis of ten species (Actinopterygii) supporting subsistence fishery in Lakshadweep waters, southern Arabian Sea

2021 ◽  
Vol 51 (3) ◽  
pp. 257-261
Author(s):  
Pentam Veli Pura Shahul Hameed ◽  
Aliyyathumada Ishyyapura Muhsin ◽  
Pathummathada Pookoya ◽  
Kutty Ranjeet
Keyword(s):  
Fish Species ◽  
Biological Information ◽  
Thunnus Albacares ◽  
Katsuwonus Pelamis ◽  
Positive Allometry ◽  
Reported Study ◽  
Weight Analysis ◽  
Total Fish ◽  
Commercially Important ◽  
Euthynnus Affinis

The length–weight relations of ten fish species representing eight genera and four families and that formed the backbone of the subsistence fishery in the Lakshadweep islands were estimated. These fishes which included four species of tuna [Katsuwonus pelamis (Linnaeus, 1758); Thunnus albacares (Bonnaterre, 1788); Auxis thazard (Lacepède, 1800); Euthynnus affinis (Cantor, 1849)], three species of needlefishes[Ablennes hians (Valenciennes, 1846); Tylosurus crocodilus (Péron et Leseur, 1821); Tylosurus acus melanotus (Bleeker, 1850)], two species of bait fishes [Spratelloides delicatulus (Bennett, 1832); Spratelloides gracilis (Temminck et Schlegel, 1846)], and one species of halfbeak (Hemiramphus archipelagicus Collette et Parin, 1978) contributed to 96% of the total fish landings. The samples were collected from fish landing centers of ten inhabited islands of Lakshadweep from 2015 to 2017. Katsuwonus pelamis showed isometric growth, S. delicatulus and S. gracilis exhibited positive allometry, while negative allometric growth was seen in other species. The coefficient a of the LWR ranged from 0.001 (A. hians) to 0.035 (T. albacares), while b ranged from 2.7 (T. acus melanotus) to 3.4 (S. delicatulus). The results of the presently reported study provide useful biological information on the stock of ten commercially important pelagic fish species supporting the traditional fisheries in Lakshadweep waters.

scholarly journals KOMPOSISI JENIS DAN DISTRIBUSI UKURAN IKANPELAGIS BESAR HASILTANGKAPANPANCINGULURDI SENDANGBIRU, JAWATIMUR

2017 ◽  
Vol 3 (6) ◽  
pp. 377
Author(s):  
Ria Faizah ◽  
Aisayah Aisayah
Keyword(s):  
Species Composition ◽  
Size Distribution ◽  
Landing Site ◽  
Pelagic Fish ◽  
Yellowfin Tuna ◽  
Thunnus Albacares ◽  
Bigeye Tuna ◽  
Katsuwonus Pelamis ◽  
Individual Weight ◽  
Thunnus Obesus

Sendang Biru merupakan salah satu tempat pendaratan ikan pelagis besar di Jawa Timur. Penelitian tentang komposisi jenis dan ukuran ikan pelagis besar hasil tangkapan pancing ulur yang didaratkan di PPI Pondok Dadap, Sendang Biru, Jawa Timur, dilakukan pada bulanApril dan Oktober 2010. Hasil penelitian menunjukkan hasil tangkapan pancing ulur didominasi oleh jenis tuna (Thunnus albacares dan Thunnus obesus) 45%, cakalang (Katsuwonus pelamis) sebesar 38 %, dan lainnya (marlin, lemadang, lauro) sebesar 1,7 %. Ikan tuna yang didaratkan terdiri dari jenis yellowfin tuna (Thunnus albacares) dan bigeye tuna (T. obesus) dengan ukuran panjang cagakmasing –masing berkisar antara 40 - 170 cmFL dan 40 - 140 cmFL. Berat individumasing-masing berkisar antara 0.1 - 71 kg dan 0.5 - 43 kg. Sendang Biru is one of big pelagic’s landing site in East Java. Tuna on this research are caught by handline that landing in PPI Pondok Dadap, Sendang Biru, East Java. Research on the species composition and size distribution of big pelagic fish caught by handline were carried out during April and October 2010 at Sendang Biru, East Java. The result showed that Thunnus sp. are the most landed (45%) followed by Katsuwonus pelamis (38 %) and others (Xiphias gladius, Coriphaena sp., Elagatis bipinnulatus) of 1.7 %. The dominant fork lengthof Thunnus albacares and Thunnus obesus ranged from about 40 - 170 cm and 40 – 140 cm. Individual weight ranged between 0.1 - 71 kg and 0.5 - 43 kg respectivelly.

Rapid Seafood Species Identification Using Chip-Based Capillary Electrophoresis and Protein Pattern Matching

2017 ◽  
Vol 100 (5) ◽  
pp. 1500-1510 ◽  
Author(s):  
Calvin C Walker ◽  
Cheryl L Lassitter ◽  
Shannara N Lynn ◽  
Courtney B Ford ◽  
Kevin R Rademacher ◽  
...  
Keyword(s):  
Protein Extraction ◽  
Protein Pattern ◽  
Screening Method ◽  
Fish Muscle ◽  
Protein Profiling ◽  
Rapid Screening ◽  
Important Species ◽  
Commercially Important ◽  
Species Specific ◽  
High Level

Abstract Authenticity is crucial to the seafood industry, as substitution and mislabeling have important economic, environmental, and food safety consequences. Toaddress this problem, protein profiling and softwarealgorithm techniques were developed to classify fishmuscle samples by species. The method uses water-based protein extraction, chip-based microfluidic electrophoresis (Agilent 2100 Bioanalyzer) for the analysis of high abundance fish muscle proteins, and a novel data analysis method for species-specific proteinpattern recognition. The method's performance in distinguishing commercially important fish from commonly reported substitutions was evaluated using sensitivity, specificity, and accuracy determinations with all three performance measures at >98% for commonsubstitutions. This study demonstrates that uncookedseafood products of commercially important species of catfish, snapper, and grouper can be rapidly distinguished from commonly substituted species with a high level of confidence. A tiered testing approach toseafood species verification by sequentially applying a rapid screening method and DNA testing is proposed to more effectively ensure accurate product labeling.

scholarly journals PERIKANAN TUNA YANG BERBASIS DI KENDARI, SULAWESI TENGGARA

2017 ◽  
Vol 2 (6) ◽  
pp. 299
Author(s):  
Agustinus Anung Widodo ◽  
Budi Nugraha
Keyword(s):  
Thunnus Albacares ◽  
Katsuwonus Pelamis ◽  
Thunnus Obesus

Kendari merupakan salah satu basis perikanan tangkap di Kawasan Timur Indonesia yang berhadapan langsung dengan Laut Banda. Produksi perikanan tuna di Kendari cukup besar, hal ini dikarenakan alat tangkap yang digunakan merupakan alat tangkap yang dikhususkan untuk menangkap ikan tuna, yaitu huhate, pukat cincin mini, dan pancing tonda. Pada bulan April, Agustus, dan Desember 2007 dilakukan penelitian dengan mengambil pengambilan contoh di PPS Kendari. Pengambilan contoh dilakukan secara acak terhadap kapal-kapal yang mendarat pada minggu terakhir bulan April, Agustus, dan Desember. Masing-masing jenis kapal (huhate, pukat cincin mini, dan tonda) diambil satu unit sebagai contoh. Jenis data yang diambil adalah aspek eksploitasi yang meliputi upaya, jumlah, dan jenis hasil tangkapan dan daerah penangkapan. Data lain yang dikumpulkan adalah produksi tuna tahunan dari PPS Kendari. Hasil penelitian menunjukkan bahwa produksi tuna selama 10 terakhir cenderung naik, pada tahun 2007 mencapai 8.381 ton. Daerah operasi penangkapan huhate dan pukat cincin mini meliputi perairan Sulawesi Tenggara dan Sulawesi Tengah, adapun pancing tonda sampai Laut Banda. Rata-rata CPUE huhate rata-rata 6,6 ton/trip, rata-tara CPUE pukat cincin mini 0,9 ton per setting dan rata-rata CPUE pancing tonda 1,3 ton/trip. Penangkapan tuna terjadi sepanjang tahun, puncak musim tahun 2007 terjadi pada bulan September dengan indeks mencapai 0,4. Jenis tuna yang tertangkap huhate, pukat cincin mini, maupun tonda ada empat, yaitu ikan cakalang (Katsuwonus pelamis), madidihang (Thunnus albacares), tuna mata besar (Thunnus obesus), dan tongkol (Auxis sp.). Komposisi dari keempat jenis tuna tersebut didominansi oleh ikan cakalang yaitu mencapai lebih dari 65%.

scholarly journals Filogenetik ikan tuna (Thunnus spp.) di Perairan Maluku Utara, Indonesia

2018 ◽  
Vol 18 (1) ◽  
pp. 1
Author(s):  
Nebuchadnezzar Akbar ◽  
Muhammad Aris ◽  
Muhammad Irfan ◽  
Abdurrachman Baksir ◽  
Surahman Surahman ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Control Region ◽  
Research Method ◽  
Thunnus Albacares ◽  
Neighbor Joining ◽  
Chain Reaction ◽  
Tuna Fish ◽  
Katsuwonus Pelamis ◽  
Thunnus Obesus ◽  
Polymerase Chain

The tuna fish (Thunnus spp.) is highly migratory and commercial tuna fishery. The fish tuna abudance supported ocea-nography and geography condition in North Mallucas Sea. The fishery targets catch increase on fish tuna provided a view of the need for assessment of phylogenetic tuna. The study was conducted to infer the phylogenetic in North Mollucas Sea. The research method was PCR-Sequensing. Moleculer analysis included extraction, Polymerase Chain Reaction (PCR), electrophoresis and DNA sequencing in control region mtDNA locus. Phylogenetic reconstructed with Neigbor joining with Kimura 2-parameter model using MEGA5. The result showed that four clade (bigeye, yellowfin, alalunga and skipjack). Genetic distance between bigeye with yellowfin was (0.084), bigeye with alalunga (0.163), ye-llowfin with alalunga (0.174), bigeye with skipjack (0.294), skipjack with alalunga (0.312) and yellowfin with skipjack (0.297). The overall result showed significant genetic different. That information explain about one populations species tuna. The tuna phylogeography unlimitedin geographic distributions. AbstrakIkan tuna (Thunnus spp.) adalah ikan pelagis yang memiliki kemampuan ruaya dan nilai komersial. Kondisi oseanogra-fis dan letak geografis mendukung kelimpahan stok sumber daya ikan tuna di Perairan Maluku Utara. Aktifitas penang-kapan yang meningkat memberikan pandangan perlu adanya pengkajian filogenetik ikan tuna. Penelitian ini bertujuan untuk memperoleh informasi filogenetik ikan tuna di perairan Maluku Utara. Metode yang digunakan adalah metode PCR-Sekuensing pada lokus mtDNA control region. Analisis molekuler meliputi ekstraksi, Polymerase Chain Reaction (PCR), elektroforesis dan sekuensing DNA. Rekonstruksi pohon filogenetik dengan metode Neighbor joining dengan model evolusi Kimura 2-parameter dilakukan menggunakan aplikasi MEGA5. Hasil penelitian menemukan empat clade spesies ikan tuna yang berbeda (tuna mata besar, sirip kuning, alalunga, dan cakalang). Jarak genetik tuna mata besar (Thunnus obesus) dengan sirip kuning (Thunnus albacares) adalah 0,084; tuna mata besar dengan tuna alalunga (Thunnus albacore) adalah 0,163; tuna sirip kuning dengan tuna alalunga sebesar 0,174; tuna mata besar dengan caka-lang (Katsuwonus pelamis) adalah 0,294; cakalang dengan tuna alalunga adalah 0,312; dan tuna sirip kuning dengan cakalang adalah 0,297. Semua hasil menunjukkan perbedaan genetik signifikan. Namun dapat dijelaskan bahwa spesies tuna berasal dari satu keturunan. Filogeografi tuna tidak memiliki batas distribusi yang nyata spesies.

scholarly journals NEMATODOS ANISAKIDAE Y RAPHIDASCARIDIDAE PARÁSITOS DE ATUNES (PERCIFORMES: SCOMBRIDAE) DEL ESTADO DE RÍO DE JANEIRO, BRASIL

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Marcelo Knoff ◽  
Michelle Cristie Gonçalves da Fonseca ◽  
Nilza Nunes Felizardo ◽  
Antonia Lúcia Dos Santos ◽  
Sérgio Carmona De São Clemente ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Thunnus Albacares ◽  
Thunnus Thynnus ◽  
Katsuwonus Pelamis ◽  
Thunnus Obesus ◽  
Auxis Thazard ◽  
Cabo Frio

Para el presente estudio, fueron adquiridos 276 atunes, entre enero de 2000 y diciembre de 2002, en mercados de peces, siendo 107 ejemplares de Katsuwonus pelamis (Linnaeus, 1758), 5 Auxis thazard (Lacepède, 1800), 92 Thunnus albacares (Bonnaterre, 1788), 30 Thunnus atlanticus (Lesson, 1830), 25 Thunnus obesus (Lowe, 1839), y 17 Thunnus thynnus (Linnaeus, 1758) pescados en el municipio de Cabo Frio, Estado de Rio de Janeiro, Brasil. Cincuenta y seis (20,28%) de los peces fueron parasitados por lo menos con una especie de parásito. Se colectaron un total de 196 nemátodos larvarios y fueron identificados como: Anisakis (Rudolphi, 1809, det. Krabbe, 1878), A. physeteris Baylis 1923, Contracaecum sp. y Raphidascaris sp. Este estudio es el primer registro en Brasil con nuevos registros para los hospederos de A. simplex en T. albacares, T. atlanticus y T. obesus, de A. physeteris en T. albacares, de Contracaecum sp. en T. atlanticus y T. obesus y de Raphidascaris sp. en T. albacares y T. obesus. Se presentaron sus índices parasitarios de prevalencia, intensidad/intensidad media, abundancia/abundancia media y el rango de la variación de la infección. Los nemátodos se colectaron en la mayoría de los cinco sitios de infección, estómago, intestino, ciego, hígado y cavidad abdominal. A. simplex parasitando T. obesus presentó el mayor nivel de prevalencia (32%), intensidad media (4,6) y abundancia media (1,48); y K. pelamis presentó el mayor rango de la variación de la infección (1-17 parásitos por pez) y el número de sitios de infección (estómago, intestino, ciego, hígado y cavidad abdominal). Se evaluó la importancia de la presencia de larvas de nematodos en estos peces para la salud pública.

scholarly journals Isolation and characterization of 10 polymorphic microsatellite loci for the endangered Galapagos-endemic whitespotted sandbass (Paralabrax albomaculatus)

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e1253 ◽  
Author(s):  
Alicia C. Bertolotti ◽  
Sarah M. Griffiths ◽  
Nathan K. Truelove ◽  
Stephen J. Box ◽  
Richard F. Preziosi ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Marine Reserve ◽  
Iucn Red List ◽  
Closely Related Species ◽  
Important Species ◽  
Isolation And Characterization ◽  
Commercially Important ◽  
Paired End Sequencing ◽  
Galapagos Marine Reserve

The white-spotted sandbass (Paralabrax albomaculatus)is a commercially important species in the Galapagos Marine Reserve, but is classified as endangered in the IUCN Red List. For this study, 10 microsatellite loci were isolated and characterized using Illumina paired-end sequencing. These loci can be used for genetic studies of population structure and connectivity to aid in the management of the white-spotted sandbass and other closely-related species. The 10 characterized loci were polymorphic, with 11–49 alleles per locus, and observed heterozygosity ranged from 0.575 to 0.964. This set of markers is the first to be developed for this species.

scholarly journals Identification of yellowfin tuna (Thunnus albacares), mackerel tuna (Euthynnus affinis), and skipjack tuna (Katsuwonus pelamis) using deep learning

2021 ◽  
Vol 944 (1) ◽  
pp. 012009
Author(s):  
I Ayuningtias ◽  
I Jaya ◽  
M Iqbal
Keyword(s):  
Deep Learning ◽  
High Accuracy ◽  
Yellowfin Tuna ◽  
Thunnus Albacares ◽  
Skipjack Tuna ◽  
Learning Methods ◽  
Katsuwonus Pelamis ◽  
Economic Values ◽  
Euthynnus Affinis

Abstract Yellowfin tuna (Thunnus albacares), mackerel tuna (Euthynnus affinis), and skipjack tuna (Katsuwonus pelamis) have important economic values for the capture fisheries in Indonesia. Activities of identifying these fish and other types of tuna have been done manually, which can lead to errors and ultimately affect statistics, stock estimates, or traceability. The aim of this research is to use deep learning methods in identifying three species of tuna, specifically yellowfin tuna, mackerel tuna, and skipjack tuna. YOLO’s newest model, YOLOv5, was used to identify the fish. The number of epochs that produces the optimum accuracy value for use in the YOLOv5 model is 400. The values for training loss, accuracy, precision, recall and F1-Score when the model is learning with a total of 400 epochs are 0.000253, 95%, 98.1%, 93.9%, and 96%. Based on these results, the three species of tuna can be identified with high accuracy.

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