scholarly journals PENGARUH MEDIA DAN ZAT PENGATUR TUMBUH TERHADAP MULTIPLIKASI TUNAS SELASIH (Ocimum basilicum) IN VITRO

2020 ◽  
Vol 12 (1) ◽  
pp. 15
Author(s):  
SITTI FATIMAH SYAHID ◽  
ENDANG HADIPOENTYANTI
Keyword(s):  
Tissue Culture ◽  
Growth Regulator ◽  
Research Result ◽  
Shoot Multiplication ◽  
Ocimum Basilicum ◽  
Shoot Length ◽  
Plant Materials ◽  
Solid Media ◽  
Number Of Leaves

ABSTRAK<br />Selasih (Ocimum basilicum) merupakan salah satu tanaman<br />penghasil minyak atsiri yang berkhasiat obat maupun pestisida nabati.<br />Untuk mendukung pengembangan peningkatan ragam genetik tanaman,<br />maka dilakukan perbanyakan bahan tanaman melalui teknik kultur in vitro.<br />Penelitian dilaksanakan mulai bulan Maret sampai Desember 2004 di<br />Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Rempah dan<br />Obat Bogor. Bahan tanaman yang digunakan sebagai eksplan adalah mata<br />tunas selasih daun ungu yang berasal dari rumah kaca. Perlakuan yang<br />diuji adalah pengaruh fisik media dan beberapa taraf konsentrasi zat<br />pengatur tumbuh Benzyl Adenin (BA). Rancangan yang digunakan adalah<br />rancangan acak lengkap dalam pola faktorial (dua faktor). Faktor pertama<br />adalah fisik media (padat dan cair) dan faktor ke dua adalah konsentrasi<br />BA (0,1 ; 0,3 dan 0,5 mg/l). Setiap perlakuan terdiri dari sepuluh ulangan.<br />Parameter yang diamati adalah jumlah tunas, panjang tunas, jumlah daun<br />dan akar serta penampakan biakan secara visual, umur sembilan minggu.<br />Hasil penelitian menunjukkan bahwa penggunaan media padat yang<br />diperkaya BA 0,3 mg/l menghasilkan jumlah tunas terbanyak yang tidak<br />berbeda nyata dengan perlakuan media padat + BA 0,1 mg/l ataupun<br />media cair + BA ( 0.1 – 0.5 mg/l ). Panjang tunas hanya dipengaruhi oleh<br />perlakuan konsentrasi BA dan tunas terpanjang diperoleh pada perlakuan<br />BA 0,3 mg/l yang tidak berbeda nyata dengan BA 0,1 mg/l. Untuk<br />parameter jumlah daun dan akar tidak dipengaruhi oleh perlakuan yang<br />diuji baik fisik media maupun konsentrasi BA.<br />Kata kunci : Selasih, Ocimum basilicum, media, pengatur tumbuh,<br />multiplikasi tunas, in vitro, Jawa Barat<br />ABSTRACT<br />Effect of media and growth regulator on shoot<br />multiplication of ocimum (Ocimum basilicum) in vitro<br />Ocimum (Ocimum basilicum) is one of important essential oil plants<br />in Indonesia which is generally used as medicine or pesticide. For plant<br />development and genetic variants improvement, tissue culture propagation<br />was conducted. The studies was conducted in March to December 2004 at<br />the Tissue Culture Laboratory of the ISMECRI Bogor. Plant materials<br />used were ocimum explants apical shoot taken from a green house.<br />Treatments tested were the effect of physical media and concentration<br />level of Benzyl Adenin (BA). The experiment used a completely<br />randomized design with two factors. First factor was physical media (solid<br />and liquid) and the second factor was BA concentrations (0.1 ; 0.3 and 0.5<br />mg/l ). Each treatment consisted of ten replications. The parameters<br />observed were number of shoots, shoot length, number of leaves and roots<br />and culture performance, nine weeks after culturing. Research result<br />showed that the use of solid media in combination with 0.3 mg/l BA was<br />the best media for shoot multiplication of ocimum in vitro and it was not<br />significantly different with liquid medium enriched with BA (0.1 -0.5<br />mg/l). Shoot length was only affected by BA concentration and the longest<br />shoot was obtained by BA 0.3 mg/l but it was not significantly different<br />with BA 0.1 mg/l. Both treatments had no effect on the number of leaves<br />and roots.<br />Key words : Ocimum, Ocimum basilicum, media, growth regulator, shoot<br />multiplication, in vitro, West Java

scholarly journals PENGGUNAAN AIR KELAPA SEBAGAI ZAT PENGATUR TUMBUH PADA MULTIPLIKASI TUNAS TEMULAWAK (Curcuma xanthorrhiza Roxb.) IN VITRO

2020 ◽  
Vol 16 (4) ◽  
pp. 135
Author(s):  
DELIAH SESWITA
Keyword(s):  
Tissue Culture ◽  
Growth Regulator ◽  
Culture Technique ◽  
Coconut Water ◽  
Raw Material ◽  
Plant Materials ◽  
Pests And Diseases ◽  
Shoot Number ◽  
Curcuma Xanthorrhiza

<p>ABSTRAK</p><p>Tanaman temulawak (Curcuma xanthorrhiza Roxb.) merupakansalah satu tanaman obat potensial unggulan yang memiliki khasiatmultifungsi. Rimpangnya yang berkhasiat obat mampu mengobati ber-bagai penyakit seperti kelainan pada hati/lever, kantong empedu, danpankreas. Adanya kecenderungan masyarakat ingin menggunakan pengo-batan dengan bahan alami, menjadikan permintaan benih temulawaksebagai bahan baku obat maupun industri jamu di Indonesia meningkatdengan pesat. Kondisi ini memberi peluang kepada petani sebagaipenyedia bahan tanaman. Upaya penyediaan bahan tanaman secara massaldalam waktu singkat serta bebas hama dan penyakit dapat dilakukanmelalui teknik kultur jaringan. Teknik ini dibatasi oleh tingginya biayaperbanyakan, di antaranya penggunaan bahan kimia. Oleh karena itu perludikaji penggunaan zat pengatur tumbuh (ZPT) yang berasal dari bahanalami (salah satunya adalah air kelapa) sebagai substitusi ZPT sintetik.Penelitian penggunaan air kelapa sebagai ZPT dilakukan di LaboratoriumKultur Jaringan Plasma Nutfah Pemuliaan dan Perbenihan, BalaiPenelitian Tanaman Obat dan Aromatik Bogor, dari bulan Mei sampaidengan bulan Desember 2009. Eksplan berasal dari tunas temulawak sterilhasil perbanyakan sebelumnya. Media yang digunakan adalah mediaMurashige and Skoog (MS) yang dikombinasikan dengan beberapa tarafkonsentrasi air kelapa (0, 5, 10, 15, dan 20%) sebagai substitusi ZPT danair kelapa dengan memakai millipore. Media dibuat padat, sebagaipembanding pada media MS + ZPT kimia yaitu BA1,5 mg/l. Percobaanmenggunakan rancangan acak lengkap dengan 10 ulangan. Parameteryang diuji adalah jumlah tunas, jumlah daun dan jumlah akar. Hasilpenelitian menunjukkan, tanpa komponen kimia, dengan penambah airkelapa pada berbagai konsentrasi pada media dasar MS, berhasilmembentuk tunas, daun dan akar. Jumlah tunas terbanyak didapat padakombinasi media dengan penambahan air kelapa 15% sebanyak 3,4 tunas,jumlah daun 2,2 daun serta jumlah akar terbanyak yaitu sebanyak 13,2akar pada umur 2 minggu. Pada kombinasi media dengan memakaimillipore, tunas terbanyak hanya 2,6 tunas, tetapi tidak berbeda nyatadengan perlakuan kontrol MS + BA 1,5 mg/l, yaitu sama-sama memiliki2,6 tunas, 3,6 daun, dan 15,4 akar.</p><p>Kata kunci : Curcuma xanthorrhiza Roxb., in vitro, air kelapa, zatpengatur tumbuh, multiplikasi in vitro</p><p>ABSTRACT</p><p>The use of Coconut Water as Growth Regulator onMultiplication of Java Turmeric Buds (Curcumaxanthorrhiza Roxb. ) in vitro</p><p>Java turmeric (Curcuma xanthorrhiza Roxb.) is a potentialmedicinal plant which has many uses. Its rhizome has efficacy to curevarious diseases such as disorder on lever, gall bladder and pancreas.There is a tendency that people want to use therapy by natural materials,increases demand of turmeric seed as raw material of medicine industry inIndonesia. This condition provides a chance to farmers as supplier of plantmaterials. However, up to now, the high need of plant materials causes thelimitation of supply so that their alternatives are needed for providing plantmaterials in maximum number. The part of plant material provision in highnumber and in a short time and free from pests and diseases can beconducted through tissue culture technique. However, this technique islimited by the high cost of multiplication, among others the use ofchemical materials. Therefore, the use of growth regulator originated fromnatural material as substitution of synthetic growth regulator need to beassessed, one of them is coconut water. The experiment was carried out atthe laboratory of Tissue Culture, Germ Plasm, and Plant Breeding,Indonesia for Medicinal and Aromatic Crop Research Institute, Bogorfrom May to December 2009. Explants originated from sterile turmericshoots, product of previous multiplication. Media used was Murashige andSkoog (MS) combined with several concentration levels of coconut water( 0; 5; 10; 15, and 20%) as substitution of growth regulator and coconutwater by using millipore. Solid media was used, as comparison on mediaof chemical MS + was BA1.5 mg/l. The experiment was arranged incompletely randomized design with 10 replications. Parameters observedwere the numbers of shoots, leaves and roots. Results showed that withoutchemical component, by addition of coconut water on variousconcentrations on based media of MS, produced shoots, leaves and roots.The highest shoot number obtained on combination of media and additionof coconut water 15% as many as 3.4 shoots, with the number of leaves2.2 leaves at the age of 2 weeks and the highest roots formed on 15 %coconut water as many as 13.2 roots. Whereas on combination of mediawith millipore, the highest shoots were only 2.6 shoots, however it was notsignificantly different from treatment of control MS + BA 1.5 mg/l, itproduced 2.6 shoots,3.6 leaves and 15.4 roots.</p><p>Key words : Curcuma xanthorrhiza Roxb., in vitro, coconut water,growth regulator, multiplication in vitro</p>

scholarly journals Perbanyakan tanaman kina Cinchona ledgeriana Moens. dan C. succirubra Pavon melalui penggandaan tunas aksiler Propagation of cinchona plant Cinchona ledgeriana Moens. and C. succirubra Pavon through axillary buds multiplication

2016 ◽  
Vol 72 (1) ◽  
Author(s):  
. JOKO-SANTOSO ◽  
Nurita TORUAN-MATHIUS ◽  
U SASTRAPRAWIRA ◽  
G SURYATMANA ◽  
D SAODAH
Keyword(s):  
Tissue Culture ◽  
Rice Husk ◽  
Shoot Multiplication ◽  
Culture Technique ◽  
Axillary Buds ◽  
Multiplication Rate ◽  
Plant Materials ◽  
Tissue Culture Technique ◽  
Cinchona Ledgeriana

SummaryCinchona ledgeriana (Ledger) and C. succirubra (Succi) were industrial commodities which their barks of the trunk  contain alkaloid   used as raw materials in pharmaceutical, food, drug and beverages and chemical industries. The problem  faced in conventional plant propagation are. incompatibilities, high numbers of death caused by transportation, limited numbers and time consume in  plant materials  production. These problems may be  overcome by axillary buds multiplication.  The aim of the experiment were to find out propagation technology of Ledger and Succi by  tissue culture technique.  Experiments were conducted in three consecutive steps, viz the effect of (i) BAP on multiplication and growth of axillary’s bud of Ledger and Succi in vitro culture, (ii) IBA on root initiation and growth, (iii)  growth medium on the growth of plantlets in  acclimatization.The design of the experiments were Complete Randomized Design with 15 (i & ii) and four (iii) replications. The treatments were (i) 0,1,2,3,4, dan 5 mg/L BAP, (ii) 0.0; 0.5; 1.0; 1.5; 2.0; dan 2.5 mg/L IBA, and (iii) mixture of soil and rice husk charcoal (1:1), mixture of soil and compost (1:1),  mixture of soil, rice husk charcoal, and  compost  (1:1:1).  Parameters measured in the experiments were (i) the initiation of buds multiplication rate twice at axillary buds at subculture.  (ii) initiation  and  roots vigor. (iii) numbers of survived  plants and plants vigor. The explant source used derived from two-month old axillary buds cultured in Murashige and Skoog (MS) medium without growth regulator. Results of the experiment showed  that the best shoot multiplication of Ledger  and Succi  was obtained from the application of 3 mg/L BAP, with buds multipli-cation rate 7 buds/explant/month for Ledger, and 3-4 buds/explants/month for succi. The best root initation and root growth were found from the application of 2 mg/L IBA. The highest percentage of survived plantlets (100%) in acclimatization was obtained from mixture of soil and rice husk charcoal (1:1) medium.  Therefore it is  concluded that tissue culture technique could be used for planlet  mass propagation    of  elite C. Ledgeriana and C. Succirubra through axillary bud multiplication.Ringkasan Tanaman kina Cinchona ledgeriana (Ledger) dan C. succirubra (Succi)  merupakan tanaman industri yang mengandung alkaloid di dalam kulit batangnya dan berguna dalam bidang industri farmasi, makanan, minuman dan kimia. Kendala yang dihadapi dalam perbanyakan tanaman kina secara konvensional dengan sistem sambung  adalah inkompatibilitas,    kematian akibat pengangkutan cukup tinggi, jumlah bahan tanam yang diproduksi sangat terbatas dan waktu penyediaan yang cukup lama. Masalah tersebut dapat diatasi dengan menggunakan teknik kultur jaringan. Penelitian ini bertujuan untuk men-dapatkan teknologi perbanyakan tanaman kina Ledger dan Succi dengan teknik kultur jaringan. Penelitian terdiri atas (i) pengaruh BAP terhadap inisiasi dan penggandaan  tunas aksilar, (ii) pengaruh IBA terhadap inisiasi serta pertum-buhan akar planlet,   dan (iii) pengaruh beberapa medium terhadap pertumbuhan planlet dalam aklimatisasi. Percobaan menggunakan Rancangan Acak Lengkap, masing-masing diulang 15 (i & ii)  dan (iii) empat kali. Peubah yang diukur untuk percobaan (i) adalah waktu inisiasi tunas dan laju penggandaan tunas aksiler pada dua  kali  subkulur. (ii)  Waktu  inisiasi  dan vigor akar. (iii) Jumlah tanaman yang bertahan hidup setelah aklimatisasi, serta vigor tanaman. Sumber eksplan yang digunakan adalah tunas aksilar dari kecambah terpilih berumur dua bulan yang dikulturkan dalam medium Murashige dan Skoog tanpa zat pengatur tumbuh. Perlakuan untuk percobaan (i) adalah 0,0; 1,0; 2,0; 3,0; 4,0 dan  5,0 mg/L BAP, (ii) adalah 0,0; 0,5; 1,0; 1,5; 2,0; dan 2,5 mg/L IBA, sedang (iii) adalah medium tanam tanah, tanah : arang sekam (1:1),  tanah : kompos (1:1), tanah : arang sekam : kompos (1:1:1). Hasil yang diperoleh menunjukkan bahwa konsentrasi BAP terbaik untuk inisiasi dan penggandaan tunas tanaman kina Ledger dan Succi adalah 3 mg/L BAP, dengan laju penggandaan tujuh tunas/eksplan/bulan untuk Ledger dan 3-4 tunas/eksplan/bulan untuk Succi. Sedang untuk perakaran diperoleh dari medium MS dengan penambahan 2 mg/L IBA. Persentase tertinggi planlet (100%) yang mampu bertahan hidup pada aklimatisasi diperoleh dari medium campuran tanah : arang sekam (1:1). Berdasarkan hasil tersebut di atas dapat disimpulkan bahwa perbanyakan tanaman kina secara in vitro untuk menghasilkan bibit bermutu dapat dilakukan melalui teknik penggandaan tunas aksiler

scholarly journals MULTIPLIKASI TUNAS DAN AKLIMATISASI PEGAGAN (Centella asiatica L.) PERIODE KULTUR LIMA TAHUN

2020 ◽  
Vol 14 (1) ◽  
pp. 30
Author(s):  
NATALINI NOVA KRISTINA ◽  
DEDI SURACHMAN
Keyword(s):  
Rice Husk ◽  
Research Result ◽  
Shoot Multiplication ◽  
Centella Asiatica ◽  
Randomized Design ◽  
Medicinal Crop ◽  
Pedicle Length ◽  
Number Of Leaves ◽  
Completely Randomized Design

ABSTRAK<br />Pegagan (Centella asiatica L.) adalah tanaman obat yang<br />mengandung zat asiaticotik sebagai obat alzaimer dan penghalus kulit.<br />Tanaman ini telah diperbanyak sejak tahun 2000. Penelitian dilakukan di<br />Laboratorium Kultur Jaringan dan Rumah Kaca Kelompok Peneliti<br />Plasma Nutfah dan Pemuliaan, Balai Penelitian Tanaman Obat dan<br />Aromatik, Bogor dari bulan Januari 2000 sampai dengan Juni 2005.<br />Penelitian ini bertujuan untuk melihat daya multiplikasi tunas setiap<br />periode subkultur dimulai dari tahun kedua sampai periode lima tahun.<br />Media yang digunakan adalah MS + BA 0,1 mg/l. Pengamatan dilakukan<br />pada jumlah tunas, jumlah daun dan visual tunas pada umur 2, 3, 4 dan 5<br />tahun pada dua periode subkultur setiap tahunnya. Penelitian disusun<br />dengan rancangan acak lengkap, masing-masing terdiri atas 10 botol yang<br />merupakan ulangan dan setiap botol terdiri atas 1 eksplan. Untuk re-media<br />terhadap tanaman yang terlihat berubah digunakan media MS + BA (0;<br />0,1; 0,2; 0,3) mg/l. Selanjutnya untuk perakaran dilakukan pada media MS<br />+ IAA (0,1 dan 0,2); MS + NAA (0,1 dan 0,2) mg/l serta MS + IBA (0,1<br />dan 0,2) mg/l. Plantlet utuh yang terbentuk selanjutnya diaklimatisasi pada<br />media tanah + pupuk kandang dan tanah + sekam dengan perbandingan 1 :<br />1. Hasil penelitian menunjukkan bahwa daya multiplikasi tunas optimum<br />terjadi pada tahun ketiga, dan memasuki tahun keempat dan kelima<br />menurun yang diiringi dengan perubahan eksplan yang terlihat pada<br />tangkai daun yang terbentuk. Akar terpanjang dan terbanyak yang<br />terbentuk didapat pada media IAA 0,2 mg/l dengan penampilan yang<br />kurus dan rapuh. Keberhasilan aklimatisasi sangat rendah, tetapi plantlet<br />mampu beregenerasi dengan baik dan terlihat tumbuh normal. Dari hasil<br />perbanyakan terlihat bahwa jumlah anakan, jumlah daun, panjang stolon<br />dan jumlah bunga lebih tinggi dibandingkan yang tumbuh pada media<br />sekam, berturut-turut : 6,77; 7,30; 46,50 cm dan 8,31. Sementara pada<br />media sekam komponen yang dominan adalah panjang tangkai daun yakni<br />9,75 cm.<br />Kata kunci : Pegagan, Centella asiatica L., multiplikasi, tunas, aklima-<br />tisasi, penyimpanan, Jawa Barat<br />ABSRACT<br />Shoot multiplication and acclimatization of gotuloca<br />(Centella asiatica L.) five years after conservation by in<br />vitro culture<br />Gotuloca (Centella asiatica L.) is a medicinal crop containing<br />asiaticotic as alzaimer and skin revitalizer. This crop has been<br />multiplicated in vitro since 2000. This research was carried out in the<br />laboratory and glasshouse of Breeding and Germplasm Group in the<br />Indonesian Medicinal and Aromatic Crops Research Institute (IMACRI)<br />from January 2000 to June 2005. The objective of the research was to find<br />out the viability of shoots multiplication after two year to five year period,<br />with media MS + BA 0.1 mg/l. The parameters observed were the number<br />of shoots, the number of leaves at the age 2, 3, 4 and 5 years from two<br />subculture periods every year. The treatments were arranged in a<br />completely randomized design, each replication consisted of 10 bottles and<br />each bottle consisted of 1 explant. After subculture the ex-plant were re-<br />media in medium MS + BA (0; 0,1; 0,2; 0,3) mg/l. The rooting media<br />before glasshouse were : MS + IAA ( 0,1 and 0,2); MS + NAA ( 0,1 and<br />0,2) mg/l; and MS + IBA ( 0,1 and 0,2) mg/l. The plantlets formed were<br />acclimatized using soil + cattle manure and soil + rice husk with<br />comparison 1:1. Research result indicated that the optimum viability<br />multiplication was achieved in the third year, and it decreased after the<br />fourth and fifth years with change in explant forming the petiole. The<br />longest and plantlet roots were formed through media IAA 0.2 mg/l with<br />brittle and thin appearance, but the plantlets were able to regenerate better<br />and grow normal. The acclimatization was not very successful but the<br />plantlets could regenerate and grew normally. The multiplication showed<br />that the number of stumps, leaves, stolons and flowers were : 6,77; 7,30;<br />46,50 cm and 8,31 respectively. In rice husk media the dominant<br />component was pedicle length 9,75 cm.<br />Key words : Gotuloca, Centella asiatica L., multiplication, shoot,<br />acclimatization, conservation, East Jav

scholarly journals Perbanyakan tanaman kina Cinchona ledgeriana Moens. dan C. succirubra Pavon melalui penggandaan tunas aksiler Propagation of cinchona plant Cinchona ledgeriana Moens. and C. succirubra Pavon through axillary buds multiplication

2016 ◽  
Vol 72 (1) ◽  
Author(s):  
. JOKO-SANTOSO ◽  
Nurita TORUAN-MATHIUS ◽  
U SASTRAPRAWIRA ◽  
G SURYATMANA ◽  
D SAODAH
Keyword(s):  
Tissue Culture ◽  
Rice Husk ◽  
Shoot Multiplication ◽  
Culture Technique ◽  
Axillary Buds ◽  
Multiplication Rate ◽  
Plant Materials ◽  
Tissue Culture Technique ◽  
Cinchona Ledgeriana

SummaryCinchona ledgeriana (Ledger) and C. succirubra (Succi) were industrial commodities which their barks of the trunk  contain alkaloid   used as raw materials in pharmaceutical, food, drug and beverages and chemical industries. The problem  faced in conventional plant propagation are. incompatibilities, high numbers of death caused by transportation, limited numbers and time consume in  plant materials  production. These problems may be  overcome by axillary buds multiplication.  The aim of the experiment were to find out propagation technology of Ledger and Succi by  tissue culture technique.  Experiments were conducted in three consecutive steps, viz the effect of (i) BAP on multiplication and growth of axillary’s bud of Ledger and Succi in vitro culture, (ii) IBA on root initiation and growth, (iii)  growth medium on the growth of plantlets in  acclimatization.The design of the experiments were Complete Randomized Design with 15 (i & ii) and four (iii) replications. The treatments were (i) 0,1,2,3,4, dan 5 mg/L BAP, (ii) 0.0; 0.5; 1.0; 1.5; 2.0; dan 2.5 mg/L IBA, and (iii) mixture of soil and rice husk charcoal (1:1), mixture of soil and compost (1:1),  mixture of soil, rice husk charcoal, and  compost  (1:1:1).  Parameters measured in the experiments were (i) the initiation of buds multiplication rate twice at axillary buds at subculture.  (ii) initiation  and  roots vigor. (iii) numbers of survived  plants and plants vigor. The explant source used derived from two-month old axillary buds cultured in Murashige and Skoog (MS) medium without growth regulator. Results of the experiment showed  that the best shoot multiplication of Ledger  and Succi  was obtained from the application of 3 mg/L BAP, with buds multipli-cation rate 7 buds/explant/month for Ledger, and 3-4 buds/explants/month for succi. The best root initation and root growth were found from the application of 2 mg/L IBA. The highest percentage of survived plantlets (100%) in acclimatization was obtained from mixture of soil and rice husk charcoal (1:1) medium.  Therefore it is  concluded that tissue culture technique could be used for planlet  mass propagation    of  elite C. Ledgeriana and C. Succirubra through axillary bud multiplication.Ringkasan Tanaman kina Cinchona ledgeriana (Ledger) dan C. succirubra (Succi)  merupakan tanaman industri yang mengandung alkaloid di dalam kulit batangnya dan berguna dalam bidang industri farmasi, makanan, minuman dan kimia. Kendala yang dihadapi dalam perbanyakan tanaman kina secara konvensional dengan sistem sambung  adalah inkompatibilitas,    kematian akibat pengangkutan cukup tinggi, jumlah bahan tanam yang diproduksi sangat terbatas dan waktu penyediaan yang cukup lama. Masalah tersebut dapat diatasi dengan menggunakan teknik kultur jaringan. Penelitian ini bertujuan untuk men-dapatkan teknologi perbanyakan tanaman kina Ledger dan Succi dengan teknik kultur jaringan. Penelitian terdiri atas (i) pengaruh BAP terhadap inisiasi dan penggandaan  tunas aksilar, (ii) pengaruh IBA terhadap inisiasi serta pertum-buhan akar planlet,   dan (iii) pengaruh beberapa medium terhadap pertumbuhan planlet dalam aklimatisasi. Percobaan menggunakan Rancangan Acak Lengkap, masing-masing diulang 15 (i & ii)  dan (iii) empat kali. Peubah yang diukur untuk percobaan (i) adalah waktu inisiasi tunas dan laju penggandaan tunas aksiler pada dua  kali  subkulur. (ii)  Waktu  inisiasi  dan vigor akar. (iii) Jumlah tanaman yang bertahan hidup setelah aklimatisasi, serta vigor tanaman. Sumber eksplan yang digunakan adalah tunas aksilar dari kecambah terpilih berumur dua bulan yang dikulturkan dalam medium Murashige dan Skoog tanpa zat pengatur tumbuh. Perlakuan untuk percobaan (i) adalah 0,0; 1,0; 2,0; 3,0; 4,0 dan  5,0 mg/L BAP, (ii) adalah 0,0; 0,5; 1,0; 1,5; 2,0; dan 2,5 mg/L IBA, sedang (iii) adalah medium tanam tanah, tanah : arang sekam (1:1),  tanah : kompos (1:1), tanah : arang sekam : kompos (1:1:1). Hasil yang diperoleh menunjukkan bahwa konsentrasi BAP terbaik untuk inisiasi dan penggandaan tunas tanaman kina Ledger dan Succi adalah 3 mg/L BAP, dengan laju penggandaan tujuh tunas/eksplan/bulan untuk Ledger dan 3-4 tunas/eksplan/bulan untuk Succi. Sedang untuk perakaran diperoleh dari medium MS dengan penambahan 2 mg/L IBA. Persentase tertinggi planlet (100%) yang mampu bertahan hidup pada aklimatisasi diperoleh dari medium campuran tanah : arang sekam (1:1). Berdasarkan hasil tersebut di atas dapat disimpulkan bahwa perbanyakan tanaman kina secara in vitro untuk menghasilkan bibit bermutu dapat dilakukan melalui teknik penggandaan tunas aksiler

scholarly journals Influence of Cytokinins in Combination with GA3on Shoot Multiplication and Elongation of Tea Clone Iran 100 (Camellia sinensis(L.) O. Kuntze)

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Reza Azadi Gonbad ◽  
Uma Rani Sinniah ◽  
Maheran Abdul Aziz ◽  
Rosfarizan Mohamad
Keyword(s):  
Camellia Sinensis ◽  
Woody Plants ◽  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Liquid Form ◽  
Solid Media

The use ofin vitroculture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis(L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA3) on shoot multiplication and elongation was assessed. The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated. The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA3. TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L. Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

scholarly journals Optimation of In vitro Lateral Shoots Multiplication of Papaya (Carica papaya L.) “Callina” with BAP and NAA

2017 ◽  
Vol 4 (3) ◽  
pp. 102-107
Author(s):  
Darda Efendi ◽  
Mirza R Putra
Keyword(s):  
Carica Papaya ◽  
Clonal Propagation ◽  
Block Design ◽  
Shoot Multiplication ◽  
Plant Materials ◽  
Randomized Block Design ◽  
Lateral Shoots ◽  
Shoot Production ◽  
Set Up

Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.

scholarly journals Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

2015 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Erna Sinaga ◽  
Megayani Sri Rahayu ◽  
Awang Maharijaya
Keyword(s):  
Tissue Culture ◽  
Drought Tolerance ◽  
In Vitro Selection ◽  
Solanum Melongena ◽  
Survival Percentage ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>

scholarly journals Mass Propagation of Nauclea diderrichii (de Willd. & Th. Dur) Merr. Seedlings through Tissue Culture Technique

2021 ◽  
Vol 31 (1) ◽  
pp. 51-60
Author(s):  
RI Oyediran ◽  
JO Afolabi ◽  
DB Olomola ◽  
FO Akanni
Keyword(s):  
Tissue Culture ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Culture Technique ◽  
Seedling Production ◽  
Mass Propagation ◽  
In Vitro Plantlets ◽  
Number Of Leaves ◽  
Nauclea Diderrichii

Nauclea diderrichii is a tree species of economic importance. However, its plantation establishment is limited by inadequate seedling production. Hence, there is ample scope of tissue culture for its mass propagation. Its in vitro plantlets development as affected by media strengths indicated that 100 % seed germination was obtained in full MS basal medium while the least (3.35 %) was from quarter-strength at 8 Weeks after inoculation (WAI). The effects of BAP and NAA assessed on the growth of its sub-cultured plantlets showed that highest number of leaves (17) and adventitious shoots (3) were obtained from MS basal medium supplemented with 0.1 mg/l BAP only. Whereas, highest shoot length (3.61 cm) and average number of roots (5/plantlet) were obtained from the same medium without hormone(s) at 8 WAI. Further sub-culturing into MS with 0.05 mg/l NAA resulted into plantlets having optimum shoot and massive root growth ready for acclimatization in 6 WAI. The plantlets were successfully acclimatized using coconuthusk/ topsoil mixture with 90 % survival. Plant Tissue Cult. & Biotech. 31(1): 51-60, 2021 (June)

Effects of Different Plant Growth Regulator on in vitro Shoot Multiplication of Chrysanthemum morifolium

2003 ◽  
Vol 3 (6) ◽  
pp. 553-560 ◽  
Author(s):  
M.Z. Karim . ◽  
M.N. Amin . ◽  
M.A.K. Azad . ◽  
F. Begum . ◽  
M.M. Rahman . ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Shoot Multiplication ◽  
Chrysanthemum Morifolium

scholarly journals In vitro callus initiation and regeneration of potato

1970 ◽  
Vol 34 (3) ◽  
pp. 449-456 ◽  
Author(s):  
AU Haque ◽  
MA Ehsanul Samad ◽  
TL Shapla
Keyword(s):  
Key Words ◽  
Root Length ◽  
Shoot Length ◽  
Leaf Explant ◽  
Callus Initiation ◽  
Number Of Leaves ◽  
Interaction Term ◽  
Shoot Tip Explants ◽  
Internode Explants

The first experiment involving different explants and concentrations of 2,4-D and kinetin showed highly significant differences for length and weight of callus formed except interaction of callus weight. Leaf explant appeared to be best of all for callus length and weight when 1.0 mg/L 2,4-D + 0.25 mg/L kinetin concentration was used. Similarly, different explants versus different concentrations of BAP/GA3/IAA showed significant differences for shoot length and leaf number per plantlet and also for root length. However, interaction term confirmed node and node/internode explants produced better results in shoot length and number of leaves per plantlet when concentrations 1 .0 mg/L BAP + 0.1 mg/L GA3 and 1.0 mg/L BAP + 0.2 mg/L GA3, 1.0 mg/L BAP + 0.4 mg/L GA3, respectively, were used. Similarly, internode explants produced better results for root length after 21 days plantlet-1 when concentration of 1.0 mg/L IAA + 0.25 mg/L GA3 was used. Shoot tip explants also produced better results in root length after 28 days plantlet-1 when concentrations 1.0 mg/L IAA + 0.25 mg/L GA3 were used. Key Words: In vitro, callus initiation, regeneration, potato. DOI: 10.3329/bjar.v34i3.3971 Bangladesh J. Agril. Res. 34(3) : 449-456, September 2009

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