scholarly journals In vitro callus initiation and regeneration of potato

1970 ◽  
Vol 34 (3) ◽  
pp. 449-456 ◽  
Author(s):  
AU Haque ◽  
MA Ehsanul Samad ◽  
TL Shapla
Keyword(s):  
Key Words ◽  
Root Length ◽  
Shoot Length ◽  
Leaf Explant ◽  
Callus Initiation ◽  
Number Of Leaves ◽  
Interaction Term ◽  
Shoot Tip Explants ◽  
Internode Explants

The first experiment involving different explants and concentrations of 2,4-D and kinetin showed highly significant differences for length and weight of callus formed except interaction of callus weight. Leaf explant appeared to be best of all for callus length and weight when 1.0 mg/L 2,4-D + 0.25 mg/L kinetin concentration was used. Similarly, different explants versus different concentrations of BAP/GA3/IAA showed significant differences for shoot length and leaf number per plantlet and also for root length. However, interaction term confirmed node and node/internode explants produced better results in shoot length and number of leaves per plantlet when concentrations 1 .0 mg/L BAP + 0.1 mg/L GA3 and 1.0 mg/L BAP + 0.2 mg/L GA3, 1.0 mg/L BAP + 0.4 mg/L GA3, respectively, were used. Similarly, internode explants produced better results for root length after 21 days plantlet-1 when concentration of 1.0 mg/L IAA + 0.25 mg/L GA3 was used. Shoot tip explants also produced better results in root length after 28 days plantlet-1 when concentrations 1.0 mg/L IAA + 0.25 mg/L GA3 were used. Key Words: In vitro, callus initiation, regeneration, potato. DOI: 10.3329/bjar.v34i3.3971 Bangladesh J. Agril. Res. 34(3) : 449-456, September 2009

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals IDENTIFICATION OF DROUGHT TOLERANT WHEAT GENOTYPES UNDER WATER DEFICIT CONDITIONS

2016 ◽  
Vol 4 (2) ◽  
pp. 206-214 ◽  
Author(s):  
Zaid Chachar ◽  
N. A. Chachar ◽  
Q.I. Chachar ◽  
S.M Mujtaba ◽  
G.A Chachar ◽  
...  
Keyword(s):  
Water Stress ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Root Length ◽  
Dry Weight ◽  
Shoot Length ◽  
Wheat Genotypes ◽  
Maximum Reduction ◽  
Drought Tolerant

Climate change is emerging phenomena and causing frequent drought which lead to scaricity of water, which ultimately nagetively affecting wheat (Triticumaestivum L.) yield all around the world. The aim of this study was to explore the potential deought tolerant wheat genotypes for candidate genes exploration. This study was conducted during the year 2014-2015 at Plant Physiology Division, Nuclear Institute of Agriculture (NIA) Tandojam. The six wheat genotypes (cv. MT-1/13, MT-2/13, MT-3/13, MT-4/13 Chakwal-86 and Khirman) were investigated for their response at germination and seedling stage under different water stress treatments (0, -0.5, -0.75 and -1.0 MPa) in controlled conditions. The results of experiments with reference to genotypes revealed that genotype Chakwal-86 shows maximum seed germination (82.58 %), while the genotype Khirman shows maximum shoot length  (7.23 cm), root length  (15.1 cm), shoot fresh wt. (5.85 g 10-1shoots), root fresh wt.  (3.45 g 10-1roots), shoot dry wt. (1.33 g 10-1shoots), root dry wt. (0.69 g 10-1roots). Among the genotypes tested Khirman and MT-4/13 are the tolerant genotypes had the potential to perform better under drought conditions, whereas  MT-4/13 and Chakwal-86 were moderate tolerant under water stress conditions. Moreover, the genotypes i.e. MT-1/13 and MT-2/13 are the sensitive genotypes under drought environment. It is concluded from present in-vitro studies that osmotic stress significantly reduced the seed germination shoot/root length fresh and dry weight in all six wheat genotypes. The maximum reduction was found at higher osmotic stress induced by PEG-6000 (-1.0 MPa) significantly.

scholarly journals In Vitro Regeneration of Onion (Allium cepa L.) Genotypes under Salt Stress Condition

2021 ◽  
pp. 34-43
Author(s):  
Asfiqur Rahman Plabon ◽  
M. E. Hoque ◽  
Farhana Afrin Vabna ◽  
Fahima Khatun
Keyword(s):  
Salt Stress ◽  
Allium Cepa ◽  
Root Length ◽  
Stress Condition ◽  
In Vitro Regeneration ◽  
Shoot Length ◽  
Salinity Level ◽  
Salt Stress Condition ◽  
Allium Cepa L

Salinity is a major problem affecting crop production all over the world. Excessive soil salinity can reduce the productivity of many agricultural crops including many vegetables and spices. Onion is one of the most important spices in the Asiatic region which is now in high demand. The experiment was conducted to observe in vitro regeneration of onion (Allium cepa L.) under salt stress condition from September 2016 to July 2017. The experiment was conducted as two factorial (genotype and treatment) Completely Randomized Design (CRD) with 3 replications for each treatment. Shoot tip segments of three genotypes namely Faridpuri, Taherpuri and Pusa red (Indian) were cultured in MS (Murashige and skoog, 1962) media supplemented with 25, 50, 75 and 100 mM NaCl. The genotype Faridpuri gave maximum salt tolerance upto 100 mM salinity level with 10.60 cm shoot length and 1.94 cm root length having the highest relative shoot and root growth. Pusa red was found to be salinity sensitive genotype which showing lowest shoot length of 7.03 cm and root length of 0.96 cm at 100 mM NaCl treatment. However, Taherpuri was tolerant up to 100 mM salinity level with 8.14 cm shoot length and 1.25 cm root length. Both the highest fresh weight of root (54.77 mg) and dry weight of root (41.36 mg) was from the genotype Faridpuri with 25 mM NaCl treatment. However, a convenient in vitro regeneration protocol of onion genotypes under different salinity level has been developed and the genotype Faridpuri can be used for further investigation in field condition to evaluate its performance at various salinity levels.

scholarly journals Eco-friendly Management of Bacterial Wilt in Tomato Using Dried Powder of the Wild Arid-Land Medicinal Shrub Rhazya stricta

Plant Disease ◽  
2020 ◽  
Vol 104 (10) ◽  
pp. 2613-2621
Author(s):  
Raja Asad Ali Khan ◽  
Syed Sartaj Alam ◽  
Sania Hayat ◽  
Musharaf Ahmad ◽  
Asad Ali ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Bacterial Wilt ◽  
Root Length ◽  
Shoot Length ◽  
Arid Land ◽  
Effective Control ◽  
Infested Soil ◽  
Fresh Biomass ◽  
Rhazya Stricta

Bacterial wilt (BW) disease caused by Ralstonia solanacearum species complex is a devastating plant disease that inflicts heavy losses to the large number of economic host plants it infects. In this study, the potential of dried powder of the arid-land medicinal shrub Rhazya stricta to control BW of tomato was explored. Both, in vitro and in planta studies were conducted, using different concentrations of dried powder of plant parts, and applied (surface mulched or mixed) to infested soil at 0, 10, and 20 days before transplanting (DBT). Aqueous extract of leaves (16% w/v) was found to be as effective as streptomycin (100 ppm) in inhibiting the in vitro growth of R. solanacearum. As evident from the scanning electron micrograph, 16% aqueous extract of leaves produced severe morphological changes, such as rupture of the bacterial cell walls. Results from the greenhouse experiments demonstrated that the higher powder dose (succulent shoot), namely, 30 g/kg of soil mixed with infested soil 20 DBT, was found to be the most effective in controlling BW. It increased root length (cm), shoot length (cm), and plant fresh biomass (g) by 55, 42, and 40%, respectively, over control plants. Mixing of plant powder with the artificially infested (35 ml of 108 CFU/ml per kilogram of soil) pot soil was better than surface mulching. The 30 g/kg of soil dose mixed with soil increased root length (cm), shoot length (cm), and plant fresh biomass (g) of treated plants by 67, 36, and 46%, respectively, over control plants. A 37% decrease in disease severity over the control was observed with drench application of 30 g of powder per kilogram of soil applied once at 20 DBT. Our results indicated that the dried powder (30 g/kg of soil) of leaves or succulent shoots of R. stricta, thoroughly mixed with soil, 20 DBT, could act as an effective control method against BW.

scholarly journals Effects of lead on seedling growth of spesia populnea

2010 ◽  
Vol 56 (No. 4) ◽  
pp. 194-199 ◽  
Author(s):  
M. Kabir ◽  
M.Z. Iqbal ◽  
M. Shafiq ◽  
Z.R. Farooqi
Keyword(s):  
Leaf Area ◽  
Environmental Conditions ◽  
Root Length ◽  
Growth And Development ◽  
Strong Influence ◽  
Dry Weight ◽  
Shoot Length ◽  
Thespesia Populnea ◽  
Seedling Length ◽  
Number Of Leaves

The effects of lead on root, shoot and seedling length, leaf area, number of leaves, plant circumference, seedling dry weight, root/shoot and leaf area ratios of <I>Thespesia populnea</I> L. were determined in greenhouse under natural environmental conditions with and without phytotoxic metal ions at 5, 10, 15, 20, and 25 µmol/l. Lead treatments have a strong influence on the growth and development of <I>T</I>. <I>populnea</I> by reducing significantly (<I>P</I> &lt; 0.05) all the above parameters. Lead treatment at 5–25 µmol/l produced significant (<I>P</I> &lt; 0.05) effects on seedling and root length, plant circumference and seedling dry weight of <I>T</I>.<I> populnea,</I> while lead treatment at 10–25 µmol/l produced significant (<I>P</I> &lt; 0.05) effects on shoot length, number of leaves and leaf area as compared to control. Tolerance in <I>T</I>.<I> populnea</I> seedling at 25 µmol/l of lead treatment was lowest as compared to all other treatments.

scholarly journals Effect of some botanical fungicide and chemicals as forest seed dressing  on the quality of the seeds: تأثير بعض مبيدات الفطريات والكيمياويات النباتية في تلبيس بذور الغابات  على جودة البذور

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Shler Hassan Mahmood, Jalal Hama Salih Ismael
Keyword(s):  
Seed Germination ◽  
Root Length ◽  
Germination Percentage ◽  
Shoot Length ◽  
Salix Alba ◽  
Seed Dressing ◽  
Number Of Leaves ◽  
Petri Plate ◽  
Local Plants ◽  
The University

An experiment was conducted to study the antifungal activity of different concentrations aqueous extracts from three local plants, Hypericum triquetrifolium, Salix alba, Salvia rosmarinus and also different concentrations of fungicide (Captan) and Salicylic acid were evaluated for their efficacy as seed dressing on Catalpa and Albizia that seeds were soaked separately for 30 mints in various concentrations of aqueous extract and fungicide. Forest seeds were grown in pots which were inoculated by Sclerotinia sclerotiorum and Fusarium oxysporum that one petri plate/ pot in 2018 in a greenhouse at the University of Sulaimani–College of Agricultural Sciences Engineering. The results showed that seed germination percentage, shoot length, root length and number of leaves was significantly (p≤0.05) increased with both the treatments. Captan (0.5%) was more effective in comparison with Captan (0.3%) and seed germination, shoot length, root length and number of leaves was gradually increased with increase of the doses of fungicides. It can be suggested that fungicide Captan have antifungal effect and can be used to treat seed against seed borne pathogens of forest, increase seed germination and shoot length, root length and number of leaves.

scholarly journals IN VITRO PROPAGATION OF ALGERIAN IVY (HEDERA CANARIENSIS L.)

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1123g-1124
Author(s):  
Karim H. Al-Juboory ◽  
David J. Williams
Keyword(s):  
Root Length ◽  
In Vitro Propagation ◽  
Inverse Relationship ◽  
Basal Medium ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Tip Explants ◽  
Strength Medium

Shoot tip explants of Algerian Ivy Heder a canariensis were cultured on MS basal medium supplemented with a combination of salt strength and NAA and IBA. More roots per explant developed on full salt strength medium combined with NAA. The most roots per explant were obtained with a combination of IBA and 1/4 MS salt. There was an inverse relationship between an increase in IBA or NAA concentration and root length and number. Shoots proliferated better on full MS salt combined with NAA and IBA. The highest level of NAA (40 uM) and 0.1 uM TDZ produced the most shoots and roots, the longest roots, the highest rooting percentage, the largest plants with the most leaves and the best callus quality per explant. The leaves from in vitro were cultured on MS medium with varying levels of Thidiazuron (TDZ) and NAA in the presence of light produced the highest number of roots.

scholarly journals Salinity-Induced Alterations in the Growth, Membranes and Osmolytes of Musa spp. cv. Caipira (AAA) During Micropropagation

2019 ◽  
Vol 11 (6) ◽  
pp. 171
Author(s):  
Yuri Lima Melo ◽  
Isabele Aragão Gomes Trindade ◽  
Monique Cristina Simão Lopes ◽  
Cibelley Vanúcia Santana Dantas ◽  
Josemir Moura Maia ◽  
...  
Keyword(s):  
Survival Rate ◽  
Plant Height ◽  
Exposure Time ◽  
Root Length ◽  
Growth Traits ◽  
In Vitro Rooting ◽  
Ms Medium ◽  
Musa Spp ◽  
Number Of Leaves

The aim of this study was to determine the concentration and exposure time to NaCl suitable for the micropropagation of banana, through the analysis of growth traits. Banana propagules were inoculated in MS medium with different concentrations of NaCl (0; 50; 75 and 100 mM) for 120 days (multiplication and rooting, 60 days each), with monthly subcultures. These propagules were measured for plant height, number of leaves, sprouting rate, average number of formed propagules, rooting rate, root length and survival rate. After 30 days, NaCl reduced sprouting rate at multiplication; the number of leaves, rooting rate and root length in rooting; and the height and propagules number in both phases. After 60 days, the NaCl affected the sprouting rate and propagules number in the multiplication; length of root in rooting; and the height and number of leaves in both phases. After 120 days, the reduction in the survival rate was proportional to the increase of NaCl in the medium. Thus, it is concluded that NaCl reduces most of the growth traits and the treatments with 75 and 100 mM NaCl affected multiplication and in vitro rooting more intensely.

scholarly journals The Growth Improvement of Grammatophyllum scriptum (Lindl.) Bl. In Vitro Plantlet using Photoautotrophic Micropropagation System

2017 ◽  
Vol 21 (2) ◽  
pp. 109 ◽  
Author(s):  
Aries Bagus Sasongko ◽  
Asruwaidah Fatumi ◽  
Ari Indrianto
Keyword(s):  
Carbon Dioxide ◽  
Root Length ◽  
Unit Area ◽  
Leaf Length ◽  
Growth Increase ◽  
Photoautotrophic Micropropagation ◽  
Average Quantity ◽  
Chlorophyll A And B ◽  
Number Of Leaves

To improve the growth of Grammatophyllum scriptum (Lindl.) Bl. in vitro plantlet, a photoautotrophic micropropagation system (PMS) was developed by growing in vitro plantlet on VW medium with varying concentration of sucrose (0, 5, 10, and 20 g/L) and additional carbon dioxide from the air (bottle covered with cap or filter). The result showed that the leaf length would increase up to 6.5 cm with PMS and it would keep growing by the adding of 5 g/L sucrose. Average number of leaves increased by 6.7 strands with PMS and the addition of sucrose increased the average quantity of leaves up to 7.7 strands. Average number and root length would increase with PMS and would even increase more with 5 g/L sucrose addition. PMS with 5 g/L sucrose can increase chlorophyll a and b concentration. The number of stomata per unit area in PMS was lower than closed culture. This shows that PMS can increase the growth of G. scriptum in vitro plantlet and the growth increase would be effective if it is combined with sucrose addition.

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