scholarly journals PENGGUNAAN AIR KELAPA SEBAGAI ZAT PENGATUR TUMBUH PADA MULTIPLIKASI TUNAS TEMULAWAK (Curcuma xanthorrhiza Roxb.) IN VITRO

2020 ◽  
Vol 16 (4) ◽  
pp. 135
Author(s):  
DELIAH SESWITA
Keyword(s):  
Tissue Culture ◽  
Growth Regulator ◽  
Culture Technique ◽  
Coconut Water ◽  
Raw Material ◽  
Plant Materials ◽  
Pests And Diseases ◽  
Shoot Number ◽  
Curcuma Xanthorrhiza

<p>ABSTRAK</p><p>Tanaman temulawak (Curcuma xanthorrhiza Roxb.) merupakansalah satu tanaman obat potensial unggulan yang memiliki khasiatmultifungsi. Rimpangnya yang berkhasiat obat mampu mengobati ber-bagai penyakit seperti kelainan pada hati/lever, kantong empedu, danpankreas. Adanya kecenderungan masyarakat ingin menggunakan pengo-batan dengan bahan alami, menjadikan permintaan benih temulawaksebagai bahan baku obat maupun industri jamu di Indonesia meningkatdengan pesat. Kondisi ini memberi peluang kepada petani sebagaipenyedia bahan tanaman. Upaya penyediaan bahan tanaman secara massaldalam waktu singkat serta bebas hama dan penyakit dapat dilakukanmelalui teknik kultur jaringan. Teknik ini dibatasi oleh tingginya biayaperbanyakan, di antaranya penggunaan bahan kimia. Oleh karena itu perludikaji penggunaan zat pengatur tumbuh (ZPT) yang berasal dari bahanalami (salah satunya adalah air kelapa) sebagai substitusi ZPT sintetik.Penelitian penggunaan air kelapa sebagai ZPT dilakukan di LaboratoriumKultur Jaringan Plasma Nutfah Pemuliaan dan Perbenihan, BalaiPenelitian Tanaman Obat dan Aromatik Bogor, dari bulan Mei sampaidengan bulan Desember 2009. Eksplan berasal dari tunas temulawak sterilhasil perbanyakan sebelumnya. Media yang digunakan adalah mediaMurashige and Skoog (MS) yang dikombinasikan dengan beberapa tarafkonsentrasi air kelapa (0, 5, 10, 15, dan 20%) sebagai substitusi ZPT danair kelapa dengan memakai millipore. Media dibuat padat, sebagaipembanding pada media MS + ZPT kimia yaitu BA1,5 mg/l. Percobaanmenggunakan rancangan acak lengkap dengan 10 ulangan. Parameteryang diuji adalah jumlah tunas, jumlah daun dan jumlah akar. Hasilpenelitian menunjukkan, tanpa komponen kimia, dengan penambah airkelapa pada berbagai konsentrasi pada media dasar MS, berhasilmembentuk tunas, daun dan akar. Jumlah tunas terbanyak didapat padakombinasi media dengan penambahan air kelapa 15% sebanyak 3,4 tunas,jumlah daun 2,2 daun serta jumlah akar terbanyak yaitu sebanyak 13,2akar pada umur 2 minggu. Pada kombinasi media dengan memakaimillipore, tunas terbanyak hanya 2,6 tunas, tetapi tidak berbeda nyatadengan perlakuan kontrol MS + BA 1,5 mg/l, yaitu sama-sama memiliki2,6 tunas, 3,6 daun, dan 15,4 akar.</p><p>Kata kunci : Curcuma xanthorrhiza Roxb., in vitro, air kelapa, zatpengatur tumbuh, multiplikasi in vitro</p><p>ABSTRACT</p><p>The use of Coconut Water as Growth Regulator onMultiplication of Java Turmeric Buds (Curcumaxanthorrhiza Roxb. ) in vitro</p><p>Java turmeric (Curcuma xanthorrhiza Roxb.) is a potentialmedicinal plant which has many uses. Its rhizome has efficacy to curevarious diseases such as disorder on lever, gall bladder and pancreas.There is a tendency that people want to use therapy by natural materials,increases demand of turmeric seed as raw material of medicine industry inIndonesia. This condition provides a chance to farmers as supplier of plantmaterials. However, up to now, the high need of plant materials causes thelimitation of supply so that their alternatives are needed for providing plantmaterials in maximum number. The part of plant material provision in highnumber and in a short time and free from pests and diseases can beconducted through tissue culture technique. However, this technique islimited by the high cost of multiplication, among others the use ofchemical materials. Therefore, the use of growth regulator originated fromnatural material as substitution of synthetic growth regulator need to beassessed, one of them is coconut water. The experiment was carried out atthe laboratory of Tissue Culture, Germ Plasm, and Plant Breeding,Indonesia for Medicinal and Aromatic Crop Research Institute, Bogorfrom May to December 2009. Explants originated from sterile turmericshoots, product of previous multiplication. Media used was Murashige andSkoog (MS) combined with several concentration levels of coconut water( 0; 5; 10; 15, and 20%) as substitution of growth regulator and coconutwater by using millipore. Solid media was used, as comparison on mediaof chemical MS + was BA1.5 mg/l. The experiment was arranged incompletely randomized design with 10 replications. Parameters observedwere the numbers of shoots, leaves and roots. Results showed that withoutchemical component, by addition of coconut water on variousconcentrations on based media of MS, produced shoots, leaves and roots.The highest shoot number obtained on combination of media and additionof coconut water 15% as many as 3.4 shoots, with the number of leaves2.2 leaves at the age of 2 weeks and the highest roots formed on 15 %coconut water as many as 13.2 roots. Whereas on combination of mediawith millipore, the highest shoots were only 2.6 shoots, however it was notsignificantly different from treatment of control MS + BA 1.5 mg/l, itproduced 2.6 shoots,3.6 leaves and 15.4 roots.</p><p>Key words : Curcuma xanthorrhiza Roxb., in vitro, coconut water,growth regulator, multiplication in vitro</p>

scholarly journals PENGARUH AIR KELAPA TERHADAP MULTIPLIKASI TUNAS IN VITRO, PRODUKSI RIMPANG, DAN KANDUNGAN XANTHORRHIZOL TEMULAWAK DI LAPANGAN

2020 ◽  
Vol 18 (3) ◽  
pp. 125 ◽  
Author(s):  
NATALINI NOVA KRISTINA ◽  
SITTI FATIMAH SYAHID
Keyword(s):  
In Vitro Culture ◽  
Growth Medium ◽  
Growth Regulator ◽  
Block Design ◽  
Hplc Method ◽  
Coconut Water ◽  
Growth And Yield ◽  
Chemical Content ◽  
Curcuma Xanthorrhiza

<p>ABSTRAK<br />Langkah antisipatif pemenuhan kebutuhan massal benih temulawak<br />dilakukan dengan perbanyakan secara in vitro menggunakan medium<br />tumbuh yang murah mengandung air kelapa. Penelitian bertujuan untuk<br />menganalisis kandungan kimia air kelapa dan peranannya dalam multi-<br />plikasi tunas temulawak in vitro, serta pengaruhnya terhadap produksi<br />rimpang dan kandungan xanthorrizol. Penelitian dilakukan mulai Mei<br />2009 sampai Agustus 2010 di Laboratorium dan Kebun Percobaan Balai<br />Penelitian Tanaman Rempah dan Obat; serta Balai Besar Penelitian dan<br />Pengembangan Pascapanen Pertanian. Air kelapa yang digunakan berasal<br />dari kelapa muda (7-8 bulan) dan kelapa tua berumur (10-12 bulan).<br />Penelitian dilakukan secara bertahap, terdiri atas 4 kegiatan. Pertama,<br />analisis zat pengatur tumbuh, vitamin dan mineral dalam air kelapa<br />menggunakan metode HPLC. Kedua, pengaruh konsentrasi air kelapa (0,<br />5, 10, 15, 20, dan 25%) terhadap multiplikasi tunas temulawak in vitro.<br />Kegiatan dirancang secara acak kelompok, 3 ulangan. Pengamatan<br />meliputi parameter pertumbuhan. Ketiga, aklimatisasi dan kandungan<br />klorofil tanaman hasil in vitro. Keempat, pertumbuhan dan produksi<br />rimpang benih temulawak in vitro dalam pot berisi media tanah + pasir dan<br />analisis kandungan xanthorrizolnya. Rancangan penelitian acak kelompok,<br />3 ulangan, dan parameter pengamatan karakter pertumbuhan, produksi<br />rimpang, dan kandungan xanthorrizol. Hasil penelitian menunjukkan<br />bahwa air kelapa mengandung kinetin, zeatin, auksin, vitamin, mineral dan<br />sumber karbon yang berguna untuk multiplikasi tunas in vitro. Kandungan<br />kimia air kelapa muda lebih tinggi dibanding air kelapa tua. Medium<br />tumbuh mengandung air kelapa 15% terbaik dalam merangsang pertum-<br />buhan tunas in vitro (rata-rata 4,6 jumlah tunas per botol selama periode<br />awal pertumbuhan (8 minggu) sehingga dijadikan sebagai standar perba-<br />nyakan. Bibit temulawak hasil perbanyakan in vitro tumbuh baik (72%)<br />pada masa aklimatisasi, walaupun sebagian kecil ada yang menguning.<br />Kandungan klorofil a, b, dan total klorofil temulawak asal kultur in vitro<br />lebih tinggi dibandingkan dengan yang konvensional, dan bentuk<br />rimpangnya normal. Poduksi rimpang generasi awal (Vo) mencapai rata-<br />rata 320,2g, lebih rendah dibandingkan dengan rimpang konvensional<br />(800,5g). Kandungan xanthorrhizol temulawak hasil kultur in vitro lebih<br />rendah dibandingkan rimpang konvensional. Hasil penelitian mengindi-<br />kasikan potensi air kelapa sebagai zat pengatur tumbuh alami pada<br />temulawak in vitro.<br />Kata kunci: air kelapa, Curcuma xanthorrhiza, in vitro, xanthorrhizol,<br />hasil</p><p>ABSTRACT<br />Anticipated step for Java turmeric seed massal fulfillment was<br />conducted by in vitro using cheap growth medium enriched with coconut<br />water. The aim of the research was to analyse the chemical content of<br />coconut water and its role on java turmeric micropropagation in vitro and<br />their effect on yield and xanthorrhizol content. The experiement was<br />conducted from May 2009 to August 2009 at Indonesian Spices and<br />Medicinal Research Institute and Indonsian Center for Agricultural Post<br />Harvest Research and Development. The coconut water used comes from<br />young coconut (7-8 months) and old coconut (10-12 months). The research<br />consisted of four steps. First, analysis of growth regulator, vitamin and<br />sucrose from coconut water using HPLC method. Second, the effect of<br />several concentration od water coconut: 0, 5, 10, 15, 20, and 25% on in<br />vitro multiplication. The experiment was arranged in completely block<br />design with three replicates. The parameters observed were growth of<br />culture during in vitro. Third, acclimatization and chlorophyll content of<br />plant derived from in vitro and fourth, growth, and yield of java turmeric<br />seed on pot containing soil + sand as growth medium and xanthorrhizol<br />analysis. The experiment was arranged in completely block design with<br />three replicates. The parameters observed were growth characters, yield<br />and xnthorrhizol content. Result showed that coconut water contain<br />kinetin, zeatin, auksin, vitamin, mineral and carbon source which used for<br />in vitro shoots multiplication. The chemical of young coconut water was<br />higher than old coconut. The growth medium enriched with 15 % coconut<br />water gave the best result on inducing shoots in vitro (average 4.6<br />shoots/bottle during 8 weeks culture), so it’s used as multiplication<br />standard. Java turmeric seed from in vitro culture grew well (72%) on<br />acclimatization. Although, some of them were greenish.The content of a,<br />b, and total chlorophyll of java ginger from in vitro culture was higher than<br />conventional rhizome and have a normal rhizome. The production on Vo<br />(plantlet generation) around 320.2 g/plant, is lower than conventional<br />rhyzome (800.5 g). Xanthorhizol and essential oil content of Java turmeric<br />from in vitro seed were lower than conventional rhyzome. Result research<br />indicated potency of the coconut water as a nature growth regulator in<br />vitro.<br />Key words: coconut water, Curcuma xanthorrhiza, in vitro, growth,<br />xanthorrhizol, yield</p>

scholarly journals Perbanyakan tanaman kina Cinchona ledgeriana Moens. dan C. succirubra Pavon melalui penggandaan tunas aksiler Propagation of cinchona plant Cinchona ledgeriana Moens. and C. succirubra Pavon through axillary buds multiplication

2016 ◽  
Vol 72 (1) ◽  
Author(s):  
. JOKO-SANTOSO ◽  
Nurita TORUAN-MATHIUS ◽  
U SASTRAPRAWIRA ◽  
G SURYATMANA ◽  
D SAODAH
Keyword(s):  
Tissue Culture ◽  
Rice Husk ◽  
Shoot Multiplication ◽  
Culture Technique ◽  
Axillary Buds ◽  
Multiplication Rate ◽  
Plant Materials ◽  
Tissue Culture Technique ◽  
Cinchona Ledgeriana

SummaryCinchona ledgeriana (Ledger) and C. succirubra (Succi) were industrial commodities which their barks of the trunk  contain alkaloid   used as raw materials in pharmaceutical, food, drug and beverages and chemical industries. The problem  faced in conventional plant propagation are. incompatibilities, high numbers of death caused by transportation, limited numbers and time consume in  plant materials  production. These problems may be  overcome by axillary buds multiplication.  The aim of the experiment were to find out propagation technology of Ledger and Succi by  tissue culture technique.  Experiments were conducted in three consecutive steps, viz the effect of (i) BAP on multiplication and growth of axillary’s bud of Ledger and Succi in vitro culture, (ii) IBA on root initiation and growth, (iii)  growth medium on the growth of plantlets in  acclimatization.The design of the experiments were Complete Randomized Design with 15 (i & ii) and four (iii) replications. The treatments were (i) 0,1,2,3,4, dan 5 mg/L BAP, (ii) 0.0; 0.5; 1.0; 1.5; 2.0; dan 2.5 mg/L IBA, and (iii) mixture of soil and rice husk charcoal (1:1), mixture of soil and compost (1:1),  mixture of soil, rice husk charcoal, and  compost  (1:1:1).  Parameters measured in the experiments were (i) the initiation of buds multiplication rate twice at axillary buds at subculture.  (ii) initiation  and  roots vigor. (iii) numbers of survived  plants and plants vigor. The explant source used derived from two-month old axillary buds cultured in Murashige and Skoog (MS) medium without growth regulator. Results of the experiment showed  that the best shoot multiplication of Ledger  and Succi  was obtained from the application of 3 mg/L BAP, with buds multipli-cation rate 7 buds/explant/month for Ledger, and 3-4 buds/explants/month for succi. The best root initation and root growth were found from the application of 2 mg/L IBA. The highest percentage of survived plantlets (100%) in acclimatization was obtained from mixture of soil and rice husk charcoal (1:1) medium.  Therefore it is  concluded that tissue culture technique could be used for planlet  mass propagation    of  elite C. Ledgeriana and C. Succirubra through axillary bud multiplication.Ringkasan Tanaman kina Cinchona ledgeriana (Ledger) dan C. succirubra (Succi)  merupakan tanaman industri yang mengandung alkaloid di dalam kulit batangnya dan berguna dalam bidang industri farmasi, makanan, minuman dan kimia. Kendala yang dihadapi dalam perbanyakan tanaman kina secara konvensional dengan sistem sambung  adalah inkompatibilitas,    kematian akibat pengangkutan cukup tinggi, jumlah bahan tanam yang diproduksi sangat terbatas dan waktu penyediaan yang cukup lama. Masalah tersebut dapat diatasi dengan menggunakan teknik kultur jaringan. Penelitian ini bertujuan untuk men-dapatkan teknologi perbanyakan tanaman kina Ledger dan Succi dengan teknik kultur jaringan. Penelitian terdiri atas (i) pengaruh BAP terhadap inisiasi dan penggandaan  tunas aksilar, (ii) pengaruh IBA terhadap inisiasi serta pertum-buhan akar planlet,   dan (iii) pengaruh beberapa medium terhadap pertumbuhan planlet dalam aklimatisasi. Percobaan menggunakan Rancangan Acak Lengkap, masing-masing diulang 15 (i & ii)  dan (iii) empat kali. Peubah yang diukur untuk percobaan (i) adalah waktu inisiasi tunas dan laju penggandaan tunas aksiler pada dua  kali  subkulur. (ii)  Waktu  inisiasi  dan vigor akar. (iii) Jumlah tanaman yang bertahan hidup setelah aklimatisasi, serta vigor tanaman. Sumber eksplan yang digunakan adalah tunas aksilar dari kecambah terpilih berumur dua bulan yang dikulturkan dalam medium Murashige dan Skoog tanpa zat pengatur tumbuh. Perlakuan untuk percobaan (i) adalah 0,0; 1,0; 2,0; 3,0; 4,0 dan  5,0 mg/L BAP, (ii) adalah 0,0; 0,5; 1,0; 1,5; 2,0; dan 2,5 mg/L IBA, sedang (iii) adalah medium tanam tanah, tanah : arang sekam (1:1),  tanah : kompos (1:1), tanah : arang sekam : kompos (1:1:1). Hasil yang diperoleh menunjukkan bahwa konsentrasi BAP terbaik untuk inisiasi dan penggandaan tunas tanaman kina Ledger dan Succi adalah 3 mg/L BAP, dengan laju penggandaan tujuh tunas/eksplan/bulan untuk Ledger dan 3-4 tunas/eksplan/bulan untuk Succi. Sedang untuk perakaran diperoleh dari medium MS dengan penambahan 2 mg/L IBA. Persentase tertinggi planlet (100%) yang mampu bertahan hidup pada aklimatisasi diperoleh dari medium campuran tanah : arang sekam (1:1). Berdasarkan hasil tersebut di atas dapat disimpulkan bahwa perbanyakan tanaman kina secara in vitro untuk menghasilkan bibit bermutu dapat dilakukan melalui teknik penggandaan tunas aksiler

scholarly journals PENGARUH MEDIA DAN ZAT PENGATUR TUMBUH TERHADAP MULTIPLIKASI TUNAS SELASIH (Ocimum basilicum) IN VITRO

2020 ◽  
Vol 12 (1) ◽  
pp. 15
Author(s):  
SITTI FATIMAH SYAHID ◽  
ENDANG HADIPOENTYANTI
Keyword(s):  
Tissue Culture ◽  
Growth Regulator ◽  
Research Result ◽  
Shoot Multiplication ◽  
Ocimum Basilicum ◽  
Shoot Length ◽  
Plant Materials ◽  
Solid Media ◽  
Number Of Leaves

ABSTRAK<br />Selasih (Ocimum basilicum) merupakan salah satu tanaman<br />penghasil minyak atsiri yang berkhasiat obat maupun pestisida nabati.<br />Untuk mendukung pengembangan peningkatan ragam genetik tanaman,<br />maka dilakukan perbanyakan bahan tanaman melalui teknik kultur in vitro.<br />Penelitian dilaksanakan mulai bulan Maret sampai Desember 2004 di<br />Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Rempah dan<br />Obat Bogor. Bahan tanaman yang digunakan sebagai eksplan adalah mata<br />tunas selasih daun ungu yang berasal dari rumah kaca. Perlakuan yang<br />diuji adalah pengaruh fisik media dan beberapa taraf konsentrasi zat<br />pengatur tumbuh Benzyl Adenin (BA). Rancangan yang digunakan adalah<br />rancangan acak lengkap dalam pola faktorial (dua faktor). Faktor pertama<br />adalah fisik media (padat dan cair) dan faktor ke dua adalah konsentrasi<br />BA (0,1 ; 0,3 dan 0,5 mg/l). Setiap perlakuan terdiri dari sepuluh ulangan.<br />Parameter yang diamati adalah jumlah tunas, panjang tunas, jumlah daun<br />dan akar serta penampakan biakan secara visual, umur sembilan minggu.<br />Hasil penelitian menunjukkan bahwa penggunaan media padat yang<br />diperkaya BA 0,3 mg/l menghasilkan jumlah tunas terbanyak yang tidak<br />berbeda nyata dengan perlakuan media padat + BA 0,1 mg/l ataupun<br />media cair + BA ( 0.1 – 0.5 mg/l ). Panjang tunas hanya dipengaruhi oleh<br />perlakuan konsentrasi BA dan tunas terpanjang diperoleh pada perlakuan<br />BA 0,3 mg/l yang tidak berbeda nyata dengan BA 0,1 mg/l. Untuk<br />parameter jumlah daun dan akar tidak dipengaruhi oleh perlakuan yang<br />diuji baik fisik media maupun konsentrasi BA.<br />Kata kunci : Selasih, Ocimum basilicum, media, pengatur tumbuh,<br />multiplikasi tunas, in vitro, Jawa Barat<br />ABSTRACT<br />Effect of media and growth regulator on shoot<br />multiplication of ocimum (Ocimum basilicum) in vitro<br />Ocimum (Ocimum basilicum) is one of important essential oil plants<br />in Indonesia which is generally used as medicine or pesticide. For plant<br />development and genetic variants improvement, tissue culture propagation<br />was conducted. The studies was conducted in March to December 2004 at<br />the Tissue Culture Laboratory of the ISMECRI Bogor. Plant materials<br />used were ocimum explants apical shoot taken from a green house.<br />Treatments tested were the effect of physical media and concentration<br />level of Benzyl Adenin (BA). The experiment used a completely<br />randomized design with two factors. First factor was physical media (solid<br />and liquid) and the second factor was BA concentrations (0.1 ; 0.3 and 0.5<br />mg/l ). Each treatment consisted of ten replications. The parameters<br />observed were number of shoots, shoot length, number of leaves and roots<br />and culture performance, nine weeks after culturing. Research result<br />showed that the use of solid media in combination with 0.3 mg/l BA was<br />the best media for shoot multiplication of ocimum in vitro and it was not<br />significantly different with liquid medium enriched with BA (0.1 -0.5<br />mg/l). Shoot length was only affected by BA concentration and the longest<br />shoot was obtained by BA 0.3 mg/l but it was not significantly different<br />with BA 0.1 mg/l. Both treatments had no effect on the number of leaves<br />and roots.<br />Key words : Ocimum, Ocimum basilicum, media, growth regulator, shoot<br />multiplication, in vitro, West Java

scholarly journals RESPON EKSPLAN PISANG KLUTUK (Musa paradisiaca L.) TERHADAP KONSENTRASI EKSTRAK BIJI PINANG MUDA DAN AIR KELAPA MUDA SECARA IN VITRO

2021 ◽  
Vol 35 (3) ◽  
pp. 135-142
Author(s):  
Zulkifli ◽  
Selvia Sutriana
Keyword(s):  
Tissue Culture ◽  
Culture Technique ◽  
Coconut Water ◽  
Areca Nut ◽  
Planting Material ◽  
Randomized Design ◽  
Significant Difference ◽  
Single Response ◽  
Number Of Shoots

Nurseries play an important role in banana development. The tissue culture technique is an alternative to produce quality seeds in large quantities, uniform and produced in a short time and free of pathogens. Areca nut extract is an organic material that contains tannin and can replace bicycling used as a disinfectant in tissue culture activities. Coconut water is a food reserve that contains vitamins and growth substances, so it can stimulate germination. The purpose of the study was to examine the interaction and single response of klutuk banana explants to the concentration of areca nut extract and young coconut water in vitro. This research was carried out at the Biotechnology Laboratory, Faculty of Agriculture, the Islamic University of Riau with the planting material used Pisang Klutuk weevil with the method used in a factorial Completely Randomized Design (CRD) with 4 x 4.  The first factor was seed extract Young areca nut and the second factor was Young Coconut Water. The parameters observed were the percentage of live explants, the percentage of contaminated explants, the age of shoot emergence, and the number of shoots. The observation results were analyzed statistically and continued with the further test of Honest Significant Difference (HSD) at the 5% level. It was found that the best treatment was P2K2 (20% young betel nut extract (200 ml + 800 ml distilled water) and young coconut water 20 ml/liter media), where the percentage of life was 100%, the percentage of contamination was 0%, the age of shoots appeared was 37.67 days, and the number of shoots was 5 strands.

scholarly journals Perbanyakan tanaman kina Cinchona ledgeriana Moens. dan C. succirubra Pavon melalui penggandaan tunas aksiler Propagation of cinchona plant Cinchona ledgeriana Moens. and C. succirubra Pavon through axillary buds multiplication

2016 ◽  
Vol 72 (1) ◽  
Author(s):  
. JOKO-SANTOSO ◽  
Nurita TORUAN-MATHIUS ◽  
U SASTRAPRAWIRA ◽  
G SURYATMANA ◽  
D SAODAH
Keyword(s):  
Tissue Culture ◽  
Rice Husk ◽  
Shoot Multiplication ◽  
Culture Technique ◽  
Axillary Buds ◽  
Multiplication Rate ◽  
Plant Materials ◽  
Tissue Culture Technique ◽  
Cinchona Ledgeriana

SummaryCinchona ledgeriana (Ledger) and C. succirubra (Succi) were industrial commodities which their barks of the trunk  contain alkaloid   used as raw materials in pharmaceutical, food, drug and beverages and chemical industries. The problem  faced in conventional plant propagation are. incompatibilities, high numbers of death caused by transportation, limited numbers and time consume in  plant materials  production. These problems may be  overcome by axillary buds multiplication.  The aim of the experiment were to find out propagation technology of Ledger and Succi by  tissue culture technique.  Experiments were conducted in three consecutive steps, viz the effect of (i) BAP on multiplication and growth of axillary’s bud of Ledger and Succi in vitro culture, (ii) IBA on root initiation and growth, (iii)  growth medium on the growth of plantlets in  acclimatization.The design of the experiments were Complete Randomized Design with 15 (i & ii) and four (iii) replications. The treatments were (i) 0,1,2,3,4, dan 5 mg/L BAP, (ii) 0.0; 0.5; 1.0; 1.5; 2.0; dan 2.5 mg/L IBA, and (iii) mixture of soil and rice husk charcoal (1:1), mixture of soil and compost (1:1),  mixture of soil, rice husk charcoal, and  compost  (1:1:1).  Parameters measured in the experiments were (i) the initiation of buds multiplication rate twice at axillary buds at subculture.  (ii) initiation  and  roots vigor. (iii) numbers of survived  plants and plants vigor. The explant source used derived from two-month old axillary buds cultured in Murashige and Skoog (MS) medium without growth regulator. Results of the experiment showed  that the best shoot multiplication of Ledger  and Succi  was obtained from the application of 3 mg/L BAP, with buds multipli-cation rate 7 buds/explant/month for Ledger, and 3-4 buds/explants/month for succi. The best root initation and root growth were found from the application of 2 mg/L IBA. The highest percentage of survived plantlets (100%) in acclimatization was obtained from mixture of soil and rice husk charcoal (1:1) medium.  Therefore it is  concluded that tissue culture technique could be used for planlet  mass propagation    of  elite C. Ledgeriana and C. Succirubra through axillary bud multiplication.Ringkasan Tanaman kina Cinchona ledgeriana (Ledger) dan C. succirubra (Succi)  merupakan tanaman industri yang mengandung alkaloid di dalam kulit batangnya dan berguna dalam bidang industri farmasi, makanan, minuman dan kimia. Kendala yang dihadapi dalam perbanyakan tanaman kina secara konvensional dengan sistem sambung  adalah inkompatibilitas,    kematian akibat pengangkutan cukup tinggi, jumlah bahan tanam yang diproduksi sangat terbatas dan waktu penyediaan yang cukup lama. Masalah tersebut dapat diatasi dengan menggunakan teknik kultur jaringan. Penelitian ini bertujuan untuk men-dapatkan teknologi perbanyakan tanaman kina Ledger dan Succi dengan teknik kultur jaringan. Penelitian terdiri atas (i) pengaruh BAP terhadap inisiasi dan penggandaan  tunas aksilar, (ii) pengaruh IBA terhadap inisiasi serta pertum-buhan akar planlet,   dan (iii) pengaruh beberapa medium terhadap pertumbuhan planlet dalam aklimatisasi. Percobaan menggunakan Rancangan Acak Lengkap, masing-masing diulang 15 (i & ii)  dan (iii) empat kali. Peubah yang diukur untuk percobaan (i) adalah waktu inisiasi tunas dan laju penggandaan tunas aksiler pada dua  kali  subkulur. (ii)  Waktu  inisiasi  dan vigor akar. (iii) Jumlah tanaman yang bertahan hidup setelah aklimatisasi, serta vigor tanaman. Sumber eksplan yang digunakan adalah tunas aksilar dari kecambah terpilih berumur dua bulan yang dikulturkan dalam medium Murashige dan Skoog tanpa zat pengatur tumbuh. Perlakuan untuk percobaan (i) adalah 0,0; 1,0; 2,0; 3,0; 4,0 dan  5,0 mg/L BAP, (ii) adalah 0,0; 0,5; 1,0; 1,5; 2,0; dan 2,5 mg/L IBA, sedang (iii) adalah medium tanam tanah, tanah : arang sekam (1:1),  tanah : kompos (1:1), tanah : arang sekam : kompos (1:1:1). Hasil yang diperoleh menunjukkan bahwa konsentrasi BAP terbaik untuk inisiasi dan penggandaan tunas tanaman kina Ledger dan Succi adalah 3 mg/L BAP, dengan laju penggandaan tujuh tunas/eksplan/bulan untuk Ledger dan 3-4 tunas/eksplan/bulan untuk Succi. Sedang untuk perakaran diperoleh dari medium MS dengan penambahan 2 mg/L IBA. Persentase tertinggi planlet (100%) yang mampu bertahan hidup pada aklimatisasi diperoleh dari medium campuran tanah : arang sekam (1:1). Berdasarkan hasil tersebut di atas dapat disimpulkan bahwa perbanyakan tanaman kina secara in vitro untuk menghasilkan bibit bermutu dapat dilakukan melalui teknik penggandaan tunas aksiler

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

scholarly journals Production high class of micro tuber potato seeds (Solanum tuberosum L.). by Using tissue culture technique

2009 ◽  
Vol 3 (2) ◽  
pp. 99-106
Author(s):  
A.A. Al-jibouri ◽  
A.A. Al-salhay
Keyword(s):  
Tissue Culture ◽  
Solanum Tuberosum L ◽  
Culture Technique ◽  
Potato Cultivars ◽  
Condition Numbers ◽  
Mass Propagation ◽  
High Class ◽  
Dormancy Period ◽  
Elisa Technique

The aim of this investigation was produced micro tubers of four potato cultivars Premiere, Bintje, Estima and Escort in vitro. Apical meristems (0.2-0.4 mm) of potato cultivars were excised and cultured on nutrient medium and incubated at 24±2 Cº and 1000 lux light intensity for 16 hrs per day. The developing plantlets were examined serological by using ELISA technique to eliminate the viral infected plantlets. The virus-free plantlets were chopped into pieces with single bud and re cultured on fresh medium for mass propagation. For micro tubers formation in test tubes, the cultures were transferred to another medium containing a high percent of sucrose (60g/L) with different concentrations of kinetin; the cultures were incubated under 16±2 Cº and 8 hrs photoperiod. The plantlets formed micro tubers after 8-10 weeks from culturing. The results showed significant differences among cultivar’s in their response to in vitro culture and micro tubers formation. The results also showed that the kinetin concentration had significant effect on micro tubers, and 1mg/l kinetin concentration was the best. The micro tubers were stored for 10 week at 4Cº to break down the dormancy period, and gave 100% germination under nursery condition. Numbers of tubers derived from micro tubers and normal tubers of these cultivars were compared at the end of season.

scholarly journals Mass Propagation of Nauclea diderrichii (de Willd. & Th. Dur) Merr. Seedlings through Tissue Culture Technique

2021 ◽  
Vol 31 (1) ◽  
pp. 51-60
Author(s):  
RI Oyediran ◽  
JO Afolabi ◽  
DB Olomola ◽  
FO Akanni
Keyword(s):  
Tissue Culture ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Culture Technique ◽  
Seedling Production ◽  
Mass Propagation ◽  
In Vitro Plantlets ◽  
Number Of Leaves ◽  
Nauclea Diderrichii

Nauclea diderrichii is a tree species of economic importance. However, its plantation establishment is limited by inadequate seedling production. Hence, there is ample scope of tissue culture for its mass propagation. Its in vitro plantlets development as affected by media strengths indicated that 100 % seed germination was obtained in full MS basal medium while the least (3.35 %) was from quarter-strength at 8 Weeks after inoculation (WAI). The effects of BAP and NAA assessed on the growth of its sub-cultured plantlets showed that highest number of leaves (17) and adventitious shoots (3) were obtained from MS basal medium supplemented with 0.1 mg/l BAP only. Whereas, highest shoot length (3.61 cm) and average number of roots (5/plantlet) were obtained from the same medium without hormone(s) at 8 WAI. Further sub-culturing into MS with 0.05 mg/l NAA resulted into plantlets having optimum shoot and massive root growth ready for acclimatization in 6 WAI. The plantlets were successfully acclimatized using coconuthusk/ topsoil mixture with 90 % survival. Plant Tissue Cult. & Biotech. 31(1): 51-60, 2021 (June)

scholarly journals Influence of Indole acetic acid and Tryptophan on production of Vinblastine and Vincristine of Catharanthus roseous callus cells in the accumulation media of In Vitro tissue culture

2010 ◽  
Vol 7 (3) ◽  
pp. 1113-1119
Author(s):  
Baghdad Science Journal
Keyword(s):  
Tissue Culture ◽  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
High Performance ◽  
Indole Acetic Acid ◽  
Culture Technique

This study on the plant of Ain –AL Bason Catharanthus roseous showed the ability of callus cells that is produced by In Vitro culture technique and transformed to the accumulated media (MS 40gm/L sucrose ,2gm/L IAA Indole acetic acid , 0.5gm/L Tryptophan) to produce Vinblastine and Vincristine compounds. Extraction, purification and quantitive determination of Vinblastine and Vincristine compounds using High performance liquid chromatography technique (HPLC)were carried out. The results showed that the highest concentration of Vinblastine and Vincristine compounds were ( 4.653,12.5 (ppm /0.5 dry Wight respectively from transformed callus cells from MS 40 gm /L sucrose , 2 gm / L NAA Naphthaline acetic acid .

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