scholarly journals Resistensi Wereng Cokelat terhadap Insektisida yang Beredar di Sentra Produksi Padi

2016 ◽  
Vol 35 (2) ◽  
pp. 99 ◽  
Author(s):  
Baehaki S. E. ◽  
E. H. Iswanto ◽  
D. Munawar
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Field Population ◽  
Wet Season ◽  
West Java ◽  
Low Resistance ◽  
Central Java ◽  
Nilaparvata Lugens Stål ◽  
Screen House

Research on brown planthopper (BPH), (Nilaparvata lugens (Stal.) resistant to insecticide using dipping method was carried out in the wet season (WS) of 2011/2012 at Indonesian Center for Rice Research, Sukamandi. BPH field population from Sukamandi, West Java, known as Sukamandi BPH population and Juwiring, Central Java, as Juwiring BPH population, were measured their degree of resistance to insecticides to be compared with the BPH biotype 1 of screen house population. The insecticides used were imidacloprid, ethiprole, thiamethoxam, fipronil, BPMC, MIPC, buprofezin, cypermethrin and cyhalothrin. The resistance ratios (RR) were measured by LC50 of BPH from fields/LC50 of BPH from screen house. Results showed that Sukamandi BPH population was moderately resistance to imidacloprid and cypermethrin, but was low resistance to buprofezin, and was decreasing into susceptibility to fipronil, thiamethoxam and cyhalothrin, where as to ethiprole, BPMC dan MIPC the Sukamandi BPH was still susceptible. The Juwiring BPH population was low resistance to imidacloprid, buprofezin, cypermethrin and cyhalothrin, but the Juwiring BPH was decreasing into susceptibility to BPMC, ethiprole and fipronil. The population was still susceptible to thiamethoxam and MIPC.

scholarly journals RESISTANCE EVALUATION OF MODERN RICE VARIETIES TO BROWN PLANTHOPPER Nilaparvata lugens STÅL

2020 ◽  
Vol 20 (2) ◽  
pp. 157-164
Author(s):  
Eko Hari Iswanto ◽  
Dede Munawar ◽  
Rahmini Rahmini
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Field Population ◽  
Rice Varieties ◽  
Resistance Evaluation ◽  
Resistant Varieties ◽  
Endemic Areas ◽  
Loss Index ◽  
Nilaparvata Lugens Stål

Resistance evaluation of modern rice varieties to brown planthopper Nilaparvata lugens STÅL. The research was performed to understand the resistance of modern rice varieties to the field population of brown planthopper (BPH). The research studied BPH settling preference, numbers of laid eggs, and functional plant loss index (FPLI) of resistant and susceptible rice varieties. The results showed BPH were less settle and laid fewer eggs on resistant varieties. BPH significantly settled longer on TN1 than other varieties after 24 hours. The numbers of eggs laid on rice resistant varieties (Inpari 13, Inpari 31, Inpari 33, RH, and PTB33) were fewer compared to TN1. FPLI value in TN1 was the highest among the tested rice varieties. Inpari 13 had better resistance to BPH compared to Inpari 31 and Inpari 33. In managing BPH in endemic areas, we recommend Inpari 13, Inpari 31, and Inpari 33 to be planted in rotation.

scholarly journals Stability of Resistance to Imidacloprid in the Brown Planthopper (Nilaparvata lugens Stål.) from Banyumas, Central Java

2020 ◽  
Vol 24 (1) ◽  
pp. 61
Author(s):  
Danarsi Diptaningsari ◽  
Y. Andi Trisyono ◽  
Aziz Purwantoro ◽  
Arman Wijonarko
Keyword(s):  
Brown Planthopper ◽  
Management Strategies ◽  
Resistance Management ◽  
Nilaparvata Lugens ◽  
Resistance Level ◽  
Central Java ◽  
Fifth Generation ◽  
Stability Of Resistance ◽  
The Stability ◽  
Nilaparvata Lugens Stål

Imidacloprid is one of the insecticides that has been widely used to control the brown planthopper (Nilaparvata lugens Stål.). The excessive use of insecticides led to the development of insecticide resistance in N. lugens. This study was aimed to determine the stability of resistance to imidacloprid in N. lugens from Banyumas, Central Java Province. The five generations of selection increased the resistance ratio (RR) from 46.20-fold to 150.39-fold. To assess the stability of resistance to imidacloprid in N. lugens, the fifth generation was further reared for 10 generations without exposure to insecticides. The stability studies showed that resistance to imidacloprid was unstable with a decrease of resistance from 150.39-fold to 38.14-fold after 10 generations without selections. Continuous selections with imidacloprid for three generations could increase the resistance level from 150.39-fold to 216.13-fold. The unstable resistance could be managed by removing the selection pressure for a period of time or switching to insecticides with different mode of actions. Information regarding resistance stability would be useful to determine an effective resistance management strategies in N. lugens.

scholarly journals PATOGENISITAS Beauveria bassiana STRAIN STGD 7(14)2 DAN STGD 5(14)2 TERHADAP WERENG COKLAT (Nilaparvata lugens STÅL)

2018 ◽  
Vol 11 (2) ◽  
pp. 122-132
Author(s):  
Yadi Suryadi ◽  
Wartono Wartono ◽  
Dwi Ningsih Susilowati ◽  
Puji Lestari ◽  
Cyntia Nirmalasari ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Internal Transcribed Spacer ◽  
Insect Pests ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Its Rdna ◽  
Stink Bug ◽  
West Java ◽  
Nilaparvata Lugens Stål

AbstrakBeauveria spp. merupakan salah satu jamur entomopatogen yang dapat digunakan sebagai agen biokontrol terhadap berbagai serangga hama. Tujuan penelitian ini adalah untuk menguji patogenitas Beauveria spp. terhadap serangga wereng coklat (Nilaparvata lugens Stål). Isolat-isolat Beauveria spp. diisolasi dari serangga walang sangit dari Situ Gede, Bogor, Jawa Barat. Identifikasi jamur dilakukan berdasarkan analisis data sekuen dari daerah Internal Transcribed Spacer (ITS) rDNA. Hasil uji menunjukkan bahwa patogenisitas isolat Stgd 5(14)2 dan Stgd 7(14)2 menghasilkan tingkat mortalitas 100% terhadap N. lugens. Isolat Stgd 5(14)2 dan Stgd 7(14)2 memiliki nilai LT50 yang rendah, danmenyebabkan kematian yang cepat terhadap wereng coklat. Hasil analisis filogenetik menunjukkan bahwa sekuen Stgd 5(14)2 dan Stgd 7(14)2 termasuk ke dalam spesies Beauveria bassiana s.str. Hasil studi ini merupakan kajian awal terhadap patogenisitas jamur entomopatogen B. bassiana terhadap wereng coklat, dan distribusinya di pertanaman padi Situ Gede, Jawa Barat.Abstract Beauveria spp. is one of the entomopathogenic fungi that can be used as biocontrol agents against various insect pests, including brown planthopper (Nilaparvata lugens Stal). This study aimed to test the pathogenicity of Beauveria spp. against N. lugens. Beauveria spp. were isolated from the rice stink bug insects, collected from Situ Gede, Bogor, West Java. Fungal identification was carried out based on the internal transcribed spacer (ITS) rDNA analysis. The pathogenicity assay revealed that Stgd 5(14)1 and Stgd 7(14)2 isolates were virulent against N. lugens, with a mortality of 100%. The LT50 (median lethal time) determination, indicated that Stgd 5(14)2  and Stgd 7(14)2 isolates had low value indicating a faster mortality in brown planthopper insects. Phylogenetic analysis showed that the sequences of Stgd 5(14)2 and Stgd 7(14)2 belong to Beauveria bassiana s.str. This is the preliminary pathogenicity trial of entomopathogenic fungi B. bassiana against brown planthopper and their distribution in rice-growing, Situ Gede area in West Java.

scholarly journals Resistance Evaluation of Some Rice Varieties by Feeding Activity of Brown Planthopper Population in Java

2021 ◽  
Vol 5 (2) ◽  
pp. 79-87
Author(s):  
Willing Bagariang ◽  
Wayan Murdita ◽  
Suwarman ◽  
Ahmad Imroni
Keyword(s):  
Feeding Activity ◽  
Brown Planthopper ◽  
Similarity Index ◽  
Nilaparvata Lugens ◽  
Rice Varieties ◽  
Resistance Level ◽  
Central Java ◽  
Important Pest ◽  
And Cluster Analysis ◽  
Nilaparvata Lugens Stål

Brown Planthopper (BPH), Nilaparvata lugens Stal (Hemiptera: Delpachidae) is the important pest of rice. The infestation of BPH reduced the rice yield on rice producer countries in Asia. In Indonesia, the area of BPH infestation frequently fluctuated over the year. BPH directly caused hopper burn and also vector of rice grassy stunt virus (RGSV) and rice ragged stunt virus (RRSV). This study aimed to evaluate the resistance of some rice varieties to different BPH population in Java. Three BPH populations collected from Karawang (West Java), Pekalongan (Central Java) and Kendal (Central Java) were used in this study. The rice resistance was evaluated by conducting honeydew test. There were eight rice varieties tested i.e Sembada 168, Ciherang, Inpari 32, Inpari 33, IR 64, TN1, Ratu Heenati and PTB 33 with five replications. A number of 200 female adults (five females per plot) were starved for 2 hours before inserting to pot containing one seedling.  The BPH adults were allowed to feed 40-day-old plants for 48 hours. The area of honeydew excreted by BPH was measured using software Image J. We used analysis of variance (ANOVA) and cluster analysis with ward method and similarity index of Euclidean distance to analyze the rice resistance to BPH. The study indicated three groups of rice resistance level. The variety of Sembada 168 and TN1 were susceptible to BPH. Variety of Ciherang, Inpari 32, Inpari 33 and IR 64 were moderately resistant to BPH. However, PTB 33 and Ratu Heenati were strongly resistant to BPH population of Java.

scholarly journals Heat Stress Impedes Development and Lowers Fecundity of the Brown Planthopper Nilaparvata lugens (Stål)

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47413 ◽  
Author(s):  
Jiranan Piyaphongkul ◽  
Jeremy Pritchard ◽  
Jeff Bale
Keyword(s):  
Heat Stress ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Nilaparvata Lugens Stål

RNA interference-aided knockdown of a putative saccharopine dehydrogenase leads to abnormal ecdysis in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

2015 ◽  
Vol 105 (4) ◽  
pp. 390-398 ◽  
Author(s):  
P.-J. Wan ◽  
L. Yang ◽  
S.-Y. Yuan ◽  
Y.-H. Tang ◽  
Q. Fu ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Transcript Level ◽  
Nilaparvata Lugens ◽  
The Body ◽  
Double Stranded Rna ◽  
Saccharopine Dehydrogenase ◽  
Body Weights ◽  
Nilaparvata Lugens Stål ◽  
Phloem Feeding ◽  
Wing Deformation

AbstractThe brown planthopper Nilaparvata lugens is a serious phloem-feeding pest of rice in China. The current study focuses on a saccharopine dehydrogenase (SDH) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (Lys), which plays a role in insect growth and carnitine production (as a substrate). The protein, provisionally designated as NlylsSDH [a SDH derived from yeast-like symbiont (YLS) in N. lugens], had a higher transcript level in abdomens, compared with heads, wings, legs and thoraces, which agrees with YLS distribution in N. lugens. Ingestion of Nlylssdh targeted double-stranded RNA (dsNlylssdh) for 5, 10 and 15 days decreased the mRNA abundance in the hoppers by 47, 70 and 31%, respectively, comparing with those ingesting normal or dsegfp diets. Nlylssdh knockdown slightly decreased the body weights, significantly delayed the development of females, and killed approximately 30% of the nymphs. Moreover, some surviving adults showed two apparent phenotypic defects: wing deformation and nymphal cuticles remained on tips of the legs and abdomens. The brachypterours/macropterours and sex ratios (female/male) of the adults on the dsRNA diet were lowered compared with the adults on diets without dsRNA. These results suggest that Nlylssdh encodes a functional SDH protein. The adverse effect of Nlylssdh knockdown on N. lugens implies the importance of Lys in hopper development. This study provides a proof of concept example that Nlylssdh could serve as a possible dsRNA-based pesticide for planthopper control.

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