scholarly journals Antioxidant and free radical scavenging effects of Iraqi sumac ( Rhus coriaria L)

2013 ◽  
Vol 10 (3) ◽  
pp. 921-933 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antioxidant Activity ◽  
Metal Ion ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Total Antioxidant ◽  
Rhus Coriaria ◽  
Dpph Radical Scavenging ◽  
Seed Extracts

This study was carried out to evaluate the antioxidant activity of Iraqi sumac seeds (Rhus coriaria. L) (Anacardiaceae). Total phenolic compounds and flavoniods were determined in three different sumac seed extracts (SSE) (aqueous,ethanolic and methanolic extract). For extraction Antioxidant activity of SSE were evaluated by various antioxidant assays, including total antioxidant capacity, reducing power,by using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging, nitric oxide scavenging, Hydroxyl radical scavenging, and metal ion chelating activities. These various antioxidant activities were compared with ascorbic acid as a standard antioxidant.The results showed that the three(SSE), contained large amounts of phenolic and flavoniods compounds. The results also showed that the three( SSEs ) have antioxidant activities , the methanolic (SSEs) contain larger amounts of phenolic and flavoniod compounds and have a higher antioxidant activities than that of aqueous or ethanolic (SSE).These results indicated that Iraqi aqueous and alcoholic extracts of Iraqi sumac in especial methanolic) SSE( are good scavengers for reactive oxygen species(ROS) and are a potential source of natural antioxidant, that may be used in pharmaceutical or food industry .

scholarly journals Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
K. Nagendra Prasad ◽  
Jing Hao ◽  
Chun Yi ◽  
Dandan Zhang ◽  
Shengxiang Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Scavenging Activity ◽  
Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

scholarly journals Variation in antioxidant activity at two ripening stages of wild mango, Spondias pinnata (L.f.) Kurz., an underutilized fruit

2020 ◽  
Vol 7 (4) ◽  
Author(s):  
Bince Mani ◽  
Sinjumol Thomas
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Total Antioxidant Activity ◽  
Plant Origin ◽  
Nitric Oxide Radical ◽  
Total Antioxidant

Antioxidant compounds play a significant role in preventing and scavenging free-radicals by reducing oxidative stress and providing protection to humans against degenerative diseases and infections. Obviously, antioxidant molecules of plant origin are pivotal to combat the oxidative harm in cells. Present work intended to evaluate the antioxidant capacity of the fruits of Spondias pinnata. Methanol extracts of the fruits at two stages of maturity were prepared and investigated by various antioxidants analyses such as total antioxidant activity, reducing power and radical scavenging assays (DPPH, nitric oxide and hydrogen peroxide). Its total phenol, flavonoid and tannin contents were also determined. Spondias pinnata fruit extracts exhibited effective antioxidant activity and its IC50 values of the unripe fruits were 65, 66, 72.23, 83.25, 66.75 ?g/ml and ripe fruits were 124.24, 92.50, 97.66, 144.10, 72.25 ?g/ml, for total antioxidant activity, reducing power, DPPH radical scavenging, nitric oxide radical scavenging and hydrogen peroxide scavenging assays respectively. The extracts, especially unripe fruit extract had good amounts of total phenolic and flavonoid contents which might contribute the antioxidant activities considerably. It is evident from the study that the fruits of S. pinnata possessed potent antioxidant activity and it can be considered as a good dietary choice among the underutilized fruits as well as common fruits. Therefore, wild mango may be a good choice of antioxidants of plant origin for dietary and pharmaceutical uses.

scholarly journals The Role of C-8 OH on the Antioxidant Activity of Norwogonin and Isowogonin

2020 ◽  
Vol 15 (5) ◽  
pp. 1934578X2092488
Author(s):  
Qiushan Zhang ◽  
Jin Shao ◽  
Tong Zhao ◽  
Lei He ◽  
Huiping Ma ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ferrous Iron ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Iron Chelation ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Hydroxyl Group ◽  
Total Antioxidant

In the present study, the antioxidant property of 4 flavones (moslosooflavone, wogonin, isowogonin, and norwogonin) was evaluated using 6 different assays: 1,1-diphenyl-2-picrylhydrazyl (DPPH·), superoxide (O2 •−), and nitric oxide (NO) radical scavenging assays, ferrous iron chelation, reducing power, and total antioxidant capacity. The 4 flavones exhibited antioxidant activities with decreasing order as norwogonin > isowogonin >> wogonin> moslosooflavone. The present results demonstrated that norwogonin and isowogonin exhibited excellent antioxidant activity, which was mainly based on the presence of C-8 hydroxyl group.

scholarly journals In VitroAntioxidant Properties of Three Varieties ofAllium sativumL. Extracts

2010 ◽  
Vol 7 (s1) ◽  
pp. S573-S579 ◽  
Author(s):  
R. T. Narendhirakannan ◽  
K. Rajeswari
Keyword(s):  
Free Radical ◽  
Allium Sativum ◽  
Preventive Intervention ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Garlic Bulb ◽  
Total Antioxidant ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Many herbs possess antioxidant ingredients that provide efficacy by additive or synergistic activities.Allium sativumL. is a strong astringent, used for the treatment of liver and spleen diseases, rheumatism and tumors. The antioxidant activities of different concentrations of ethanolic extracts of garlic bulb of three varieties were determined by the four assaysi.e. DPPH radical scavenging assay, reducing power ability, hydrogen peroxide scavenging assay and total antioxidant capacity. Due to its natural origin and potent free-radical scavenging ability,Allium sativumL. could be used as a potential preventive intervention for free radical-mediated diseases.

scholarly journals Studies on In Vitro Antioxidant Activity of Methanolic Extract and Fractions of Ficus Hispida Lin. Fruits

2016 ◽  
Vol 8 (3) ◽  
pp. 371-380 ◽  
Author(s):  
M. S. Hossain ◽  
S. Parvin ◽  
S. Dutta ◽  
M. S. I. Mahbub ◽  
M. E. Islam
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Traditional Use ◽  
Total Antioxidant ◽  
Ficus Hispida

The present study was designed to confirm the traditional use of the fruits of Ficus hispida Linn. (Moraceae) as an antioxidant agent. Fruits of the plant extracted with methanol and crude methanol extract (CME) were further fractionated with n-hexane, chloroform, and ethyl acetate. All the fractions, n-hexane (NHF), chloroform (CHF), ethyl acetate (EAF), aqueous (AQF) and CME were preliminary screened for in vitro antioxidant activity and total phenolic and total flavonoid content. In DPPH radical scavenging assay, CME exhibited highest scavenging activity (IC50 = 11.20 µg/mL) as compared to other fractions. In this assay, IC50 of reference standard BHT was 5.10 µg/mL. The reducing power of the samples was in the order as AQF > CME > CHF > EAF > NHF. The results for hydrogen peroxide scavenging activity indicated that CME, EAF and AQF had almost the same scavenging activity except NHF. Total antioxidant capacity of CME and other fractions were ranked as CHF > AQF > CME > EAF > NHF.  In the assay of antioxidant constituents (total phenol and total flavonoids content), the CME had highest phenolic and flavonoids content. The results indicate that Ficus hispida fruits could be considered as a potential source of natural antioxidant.

scholarly journals Effect of Different Cooking Methods on Polyphenols, Carotenoids and Antioxidant Activities of Selected Edible Leaves

Antioxidants ◽  
2018 ◽  
Vol 7 (9) ◽  
pp. 117 ◽  
Author(s):  
K. Gunathilake ◽  
K. Ranaweera ◽  
H. Rupasinghe
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Leafy Vegetables ◽  
Cooking Methods ◽  
Oxygen Scavenging ◽  
Total Antioxidant ◽  
The Impact ◽  
Dpph Radical Scavenging

This study aimed to evaluate the effect of cooking (boiling, steaming, and frying) on polyphenols, flavonoids, carotenoids and antioxidant activity of six edible leaves. The total antioxidant capacity of the fresh and cooked leaves was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and singlet oxygen scavenging assays. The results revealed that frying caused a reduction in major bioactives and antioxidant activities in all leafy vegetables tested. However, steamed and boiled leaves of C. auriculata and C. asiatica have shown greater levels of polyphenols, flavonoids, and antioxidant capacity compared with fresh leaves. Polyphenol and flavonoid contents of boiled S. grandiflora and G. lactiferum were higher than that of their fresh form. Boiled and steamed O. zeylanica and S. grandiflora have shown higher carotenoids. Boiled and steamed leaves of P. edulis have shown higher antioxidant activity. The impact of cooking on the changes in bioactive concentrations and antioxidant capacities are dependent on the species and the method of cooking.

scholarly journals A New Natural Antioxidant Biomaterial from Cinnamomum osmophloeum Kanehira Leaves Represses Melanogenesis and Protects against DNA Damage

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 474 ◽  
Author(s):  
Yung-Shu Ho ◽  
Jane-Yii Wu ◽  
Chi-Yue Chang
Keyword(s):  
Dna Damage ◽  
Metal Ion ◽  
Steam Distillation ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Chemical Compositions ◽  
Melanin Synthesis

Cinnamomoum osmophloeum Kanehira (COK) is an indigenous tree species in Taiwan. Chemical compositions, antioxidant activity, mushroom tyrosinase inhibition, melanin synthesis repression, and protection against DNA damage of hydrosol from the COK leaves by steam distillation were examined. We performed 1,1-diphenyl-2-picrylhydrazyl radical scavenging, metal ion chelating, reducing power, and Trolox equivalent antioxidant capacity (TEAC) assays and determined the correlations between total phenolic contents and antioxidant activities. The findings showed that the anti-oxidative properties of COK hydrosol are closely correlated with their phenol contents. Additionally, the major constituents of hydrosol, i.e., cinnamaldehyde and benzaldehyde, had dose-dependent anti-tyrosinase effects against both monophenolase and diphenolase activities. GC-MS analysis revealed that the major bioactive components of hydrosol were trans-cinnamaldehyde (87.7%), benzaldehyde (7.0%), and cinnamyl acetate (5.3%). Moreover, we found that the hydrosol with the presence of benzaldehyde is more potent than pure cinnamaldehyde, and enhances the tyrosinase inhibitory activity of hydrosol. In kinetic analyses, Lineweaver–Burk plots and replots showed that COK hydrosol is a mixed-type inhibitor. Additionally, we found that very low doses of COK hydrosol repressed α-melanocyte-stimulating hormone-induced synthesis of microphthalmia-associated transcription factor, leading to decreased melanin synthesis in B16-F10 melanoma cells. These results demonstrated that production of hydrosol from COK leaves using steam distillation may provide a safe and efficacious source of skin-whitening agents for cosmetic and pharmaceutical applications, with antioxidant, anti-tyrosinase, anti-melanogenesis, and DNA protective activities.

scholarly journals Phenolic Compounds and Antioxidant Activity of Castanopsis phuthoensis and Castanopsis grandicicatricata

2016 ◽  
Vol 55 ◽  
pp. 77-87 ◽  
Author(s):  
Phung Thi Tuyen ◽  
Do Tan Khang ◽  
Luong The Minh ◽  
Truong Ngoc Minh ◽  
Pham Thi Thu Ha ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Phenolic Extracts

In this study, total phenolic, flavonoid contents, antioxidant capacity, and phenolic compositions of Castanopsis phuthoensis and Castanopsis grandicicatricata (Fagaceae family) were investigated. It was found that bark extracts were rich of phenolic contents, whereas leaf extracts were abundant of flavonoids. The total phenolics varied from 11.20 to 35.47 mg gallic acid equivalent g-1 dry weight (DW), and the total flavonoids were from 2.24 to 12.55 mg rutin equivalent g-1 DW. The results of antioxidant activity showed that the DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity of the free phenolic extracts were higher than the bound phenolic extracts. Regarding the reducing power and β-carotene bleaching assays, the free phenolic extracts showed remarkably strong antioxidant capacity that were similar to the levels of the standard BHT (dibutyl hydroxytoluene) did. It could be concluded that free phenolic extracts were more effective in antioxidant activities than bound phenolic extracts. A highly significant correlation between phenolic contents and antioxidant activity in extracts were observed. By HPLC analysis, seven phenolic acids were detected including gallic, p-hydroxybenzoic, vanillic, sinapic, p-coumaric, ellagic, and vanillin. Of which, gallic, ellagic, and sinapic acids were the most abundant compounds in the two species. The results suggest C. phuthoensis and C. grandicicatricata contain rich sources of natural antioxidants and phenolic compounds which are probably considered in pharmaceutical use.

scholarly journals Antioxidant Activity of Methanol Extract from Several Indonesian Green Teas

2018 ◽  
Vol 26 (1) ◽  
pp. 018
Author(s):  
Adelina Simamora ◽  
Michael Steven ◽  
Adit Widodo Santoso ◽  
Flora Rumiati ◽  
Kris Herawan Timotius
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Green Tea ◽  
Total Antioxidant Capacity ◽  
Health Effect ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Butylated Hydroxytoluene ◽  
Total Phenolic ◽  
Total Antioxidant

<p>As a common beverage, green tea is supposed to have beneficial health effect, such as antioxidant. At present, there are many green teas available in the market in Jakarta. Their quality, in their antioxidant activity, should be proved. This study aimed to investigate the antioxidant activity of extracts of green tea products commercially available in Jakarta. Four green tea samples from different factories (coded as A, B, C, and D) were selected and macerated using methanol. DPPH radical scavenging activity, reducing power ability and total antioxidant capacity were used to measure the antioxidant activity. The total phenolic content (TPC) was also determined. The studied green teas had varied TPC from 23.80  to  84.03 mG GAE/g extract, ranked as B &gt; D &gt; A &gt; C. All samples exhibited various but strong antioxidant activity by DPPH assay, even better than standards ascorbic acid and butylated hydroxytoluene (BHT). However, all extracts showed similar activities in their reducing power ability and total antioxidant capacity, with activities less than standards.<strong> </strong>These findings confirm that the quality of the Indonesian commercial green teas were heterogeneous both in TPC or DPPH scavenging capacity. But, there is an indication that they are good as an antioxidant containing beverage. </p>

scholarly journals In Vitro Assessment of Selected Medicinal Plants Used by the Bapedi Community in South Africa for Treatment of Bacterial Infections

2018 ◽  
Vol 23 ◽  
pp. 2515690X1876273 ◽  
Author(s):  
Refilwe Given Kudumela ◽  
Peter Masoko
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Bacterial Infections ◽  
Antioxidant Activities ◽  
Scientific Information ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Limpopo Province ◽  
Total Phenolic

There is very little scientific information on indigenous plants used for medicinal purposes. Therefore, the aim of the study was to evaluate the antibacterial and antioxidant activities of the leaves of Commelina africana, Elephantorrhiza elephantina, Dombeya rotundifolia, and the whole plant excluding the roots of Schkuhria pinnata indigenous medicinal plants from the Limpopo Province, which may be used for the treatment in humans infected with bacterial pathogens. The screening of different phytoconstituents using standard methods revealed the presence of terpenoids, flavonoids, tannins, and saponins. The total phenolic, tannin, and flavonoid content were estimated using spectrophotometric methods. D rotundifolia had the highest amounts of phenol (259.00 ± 2.65 mg of gallic acid equivalent [GAE]/g), tannin (330.33 ± 15.63 mg of GAE/g), and flavonoid (19.90 ± 0.75 mg of quercetin equivalent [QE]/g) content, while S pinnata had the least. All the plants had antibacterial activity against Escherichia coli and Pseudomonas aeruginosa with the bioautography method. The microbroth dilution assay revealed antibacterial activity with minimum inhibitory concentration values raining from 0.02 to 2.50 mg/mL. D rotundifolia had the highest antioxidant activity in both the free radical scavenging and ferric reducing power methods. S pinnata and C africana extracts had high antibacterial activity, while D rotundifolia had high antioxidant activity, which made them potential plants for isolation of bioactive compounds and possible application in the pharmaceutical industry.

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